Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7126-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-861AFP488 | |||
| Alexa Fluor Plus 555 | NB600-861AFP555 | |||
| Alexa Fluor Plus 594 | NB600-861AFP594 | |||
| Alexa Fluor Plus 647 | NB600-861AFP647 | |||
| Alexa Fluor Plus 680 | NB600-861AFP680 | |||
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB9626P-100UG | |||
| Carrier Free | MAB9626-SP | |||
| Carrier Free | FAB9626V-100UG | |||
| Carrier Free | FAB9626U-100UG | |||
| Carrier Free | FAB9626T-100UG | |||
| Carrier Free | FAB9626S-100UG | |||
| Carrier Free | FAB9626R-100UG | |||
| Carrier Free | FAB9626N-100UG | |||
| Carrier Free | FAB9626G-100UG | |||
![N/A Flt-3 Ligand/FLT3L [Biotin]](http://images.novusbio.com/fullsize2/DATA_Flt3_Ligand_DY427_ELISA_2021.jpg "N/A Flt-3 Ligand/FLT3L [Biotin]")
![N/A IL-17C [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17C_DY1234_ELISA_1607.jpg "N/A IL-17C [Biotin]")
![N/A u-Plasminogen Activator/Urokinase [HRP]](http://images.novusbio.com/fullsize2/DATA_uPlasminogen_Activator_DUPA00_ELISA_867.jpg "N/A u-Plasminogen Activator/Urokinase [HRP]")
![N/A u-Plasminogen Activator/Urokinase [HRP]](http://images.novusbio.com/fullsize2/u-Plasminogen_Activator_DUPA00_ELISA_204.jpg)
![N/A Cathepsin L [Biotin]](http://images.novusbio.com/fullsize2/DATA_Cathepsin_L_DY952_ELISA_2273.jpg "N/A Cathepsin L [Biotin]")
![N/A HB-EGF [HRP]](http://images.novusbio.com/fullsize2/HBEGF_DHBEG0_ELISA_1109.jpg "N/A HB-EGF [HRP]")
![N/A HB-EGF [HRP]](http://images.novusbio.com/fullsize2/HBEGF_DHBEG0_ELISA_1108.jpg)
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize2/DATA_Sonic_Hedgehog_MSHH00_ELISA_1032.jpg "N/A Sonic Hedgehog/Shh [HRP]")
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize2/Sonic_Hedgehog_MSHH00_ELISA_323.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
![N/A Oncostatin M/OSM [Biotin]](http://images.novusbio.com/fullsize2/DATA_OSM_DY49505_ELISA_2471.jpg "N/A Oncostatin M/OSM [Biotin]")
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize2/Sonic_Hedgehog_DSHH00_ELISA_1118.jpg "N/A Sonic Hedgehog/Shh [HRP]")
![N/A Sonic Hedgehog/Shh [HRP]](http://images.novusbio.com/fullsize2/Sonic_Hedgehog_DSHH00_ELISA_1116.jpg)
![N/A CXCL6/GCP-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CXCL6_DY333_ELISA_1935.jpg "N/A CXCL6/GCP-2 [Biotin]")