| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80755 | |||
| BSA Free | NBP1-19015SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4959-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08555 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59369-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08556 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98271-50ul | |||
![Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. Image from verified customer review.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Glo1a-Western-Blot-NBP1-19015-img0007.jpg "Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma cell line resistant to trabectedine. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with Glo1a mAb using anti mouse IgG Texas Red as secondary Ab. The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain. No reactivity was detected in control cells, stained without Go1a mAb. Figure is a representative of 3 independent experiments. Photo courtesy of Dr. Nagaraj, Case Western Reserve University.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Glo1a-Immunocytochemistry-Immunofluorescence-NBP1-19015-img0004.jpg)
![Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Glo1a-Western-Blot-NBP1-19015-img0003.jpg)
![Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Glo1a-Simple-Western-NBP1-19015-img0006.jpg)
![Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Glo1a-Simple-Western-NBP1-19015-img0005.jpg)
![Western Blot: Glyoxalase I Antibody [NBP1-31466] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP1-31466-img0022.jpg "Western Blot: Glyoxalase I Antibody [NBP1-31466] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody [NBP1-31466] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I protein stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31466-img0014.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody [NBP1-31466] - Human ovarian cancer. Glyoxalase I stained by Glyoxalase I antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunohistochemistry-Paraffin-NBP1-31466-img0020.jpg)
![Western Blot: Glyoxalase I Antibody [NBP1-31466] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP1-31466-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glyoxalase I Antibody [NBP1-31466] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31466_rabbit-polyclonal-glyoxalase-i-antibody-1610202419405751.jpg)
![Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Western Blot Analysis of HeLa cell lysate using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-1-Western-Blot-NBP3-07311-img0004.jpg "Western Blot: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Western Blot Analysis of HeLa cell lysate using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).")
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-1-Immunohistochemistry-Paraffin-NBP3-07311-img0003.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-1).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-1-Immunohistochemistry-Paraffin-NBP3-07311-img0002.jpg)
![Protein Array: Glyoxalase I Antibody (CPTC-GLO1-1) [NBP3-07311] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Glyoxalase I Monoclonal Antibody (CPTC-GLO1-1).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-1-Protein-Array-NBP3-07311-img0001.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:200.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-266-Immunohistochemistry-Paraffin-NBP2-43618-img0004.jpg "Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:200.")
![Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract D. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Glyoxalase I antibody [266] dilution: 1:1000.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-266-Western-Blot-NBP2-43618-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [GT266] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43618_mouse-monoclonal-glyoxalase-i-antibody-266-1610202419414592.jpg)
![Glyoxalase I antibody [GT266] detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glyoxalase I stained by Glyoxalase I antibody [GT266] (NBP2-43618) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-43618_mouse-monoclonal-glyoxalase-i-antibody-266-immunocytochemistry-immunofluorescence-2611202518261310.jpg)
![Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Western Blot analysis of Glyoxalase I-BSA Conjugate showing detection of 67 kDa Glyoxalase I protein using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Lane 1: Molecular Weight Ladder (MW). Lane 2: Glyoxalase I-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Western-Blot-NBP2-59369-img0007.jpg "Western Blot: Glyoxalase I Antibody (9F11) [NBP2-59369] - Western Blot analysis of Glyoxalase I-BSA Conjugate showing detection of 67 kDa Glyoxalase I protein using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Lane 1: Molecular Weight Ladder (MW). Lane 2: Glyoxalase I-BSA. Lane 3: BSA. Load: 0.5 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (9F11) [NBP2-59369] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom Courtesy of: Dr. Robert Burke, University of Victoria.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Immunocytochemistry-Immunofluorescence-NBP2-59369-img0002.jpg)
![Flow Cytometry: Glyoxalase I Antibody (9F11) [NBP2-59369] - Flow Cytometry analysis using Mouse Anti-Glyoxalase I Monoclonal Antibody, Clone 9F11 (NBP2-59369). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Glyoxalase I Monoclonal Antibody (NBP2-59369) at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 M H2O2 for 24 hours.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-9F11-Flow-Cytometry-NBP2-59369-img0008.jpg)
![Western Blot: Glyoxalase I Antibody [NBP2-56695] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP2-56695-img0002.jpg "Western Blot: Glyoxalase I Antibody [NBP2-56695] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody [NBP2-56695] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & cytosol.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56695-img0001.jpg)
![Western Blot: Glyoxalase I Antibody (CPTC-GLO1-3) [NBP3-07312] - Western Blot Analysis of human HeLa and HePG2 cell lysates using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-3).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-3-Western-Blot-NBP3-07312-img0003.jpg "Western Blot: Glyoxalase I Antibody (CPTC-GLO1-3) [NBP3-07312] - Western Blot Analysis of human HeLa and HePG2 cell lysates using Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-3).")
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-3) [NBP3-07312] - Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-3).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-3-Immunohistochemistry-Paraffin-NBP3-07312-img0002.jpg)
![Immunohistochemistry-Paraffin: Glyoxalase I Antibody (CPTC-GLO1-3) [NBP3-07312] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Glyoxalase I Mouse Monoclonal Antibody (CPTC-GLO1-3).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-CPTC-GLO1-3-Immunohistochemistry-Paraffin-NBP3-07312-img0001.jpg)
![Immunoprecipitation:Glyoxalase I AntibodyNBP3-29592] -Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody NBP3-29592 (lot NBP3-29592-1) used for IP at 6 µg per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I.. For blotting immunoprecipitated GLO1/Glyoxalase I, NBP3-29592 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29592_rabbit-glyoxalase-i-pab-3012202412262520.jpg "Immunoprecipitation:Glyoxalase I AntibodyNBP3-29592] -Detection of human GLO1/Glyoxalase I by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLO1/Glyoxalase Iantibody NBP3-29592 (lot NBP3-29592-1) used for IP at 6 µg per reaction. GLO1/Glyoxalase I was also immunoprecipitated by rabbit anti-GLO1/Glyoxalase I.. For blotting immunoprecipitated GLO1/Glyoxalase I, NBP3-29592 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Western Blot: Glyoxalase I Antibody (3G6Q1) [NBP3-16375] - Western blot analysis of extracts of various cell lines, using Glyoxalase I Rabbit mAb (NBP3-16375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-3G6Q1-Western-Blot-NBP3-16375-img0001.jpg "Western Blot: Glyoxalase I Antibody (3G6Q1) [NBP3-16375] - Western blot analysis of extracts of various cell lines, using Glyoxalase I Rabbit mAb (NBP3-16375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Glyoxalase I Antibody [NBP2-98271] - Anti-Glyoxalase I rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Western-Blot-NBP2-98271-img0004.jpg "Western Blot: Glyoxalase I Antibody [NBP2-98271] - Anti-Glyoxalase I rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Glyoxalase I Antibody [NBP2-98271] - Mouse Glyoxalase I was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-Mouse Glyoxalase I rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse Glyoxalase I rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 24 kDa](http://images.novusbio.com/fullsize/Glyoxalase-I-Antibody-Immunoprecipitation-NBP2-98271-img0003.jpg)