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6-His Epitope Tag: You're It

The 6-His antibody recognizes a very short amino acid sequence epitope that is widely and commonly used as a protein fusion tag, often at the N- or C-terminus of protein constructs. It is a very powerful investigative research toolfor applications such as immunochemistry, protein purification, ELISA purification, and protein localization.

Luciferase: Shining a Light to See Inside Living Animal Models

The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bioluminescence imaging (BLI) technology. Further detail can be revealed through the use of luciferase antibodies.

The Myc Epitope Tags Along...

Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them by immnoflorescence, immunoprecipitation and by Western blotting assays.

Time to Start Actin Like a Reliable 'Housekeeper'!

A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properties of smooth muscle tissues.

Customer Experience Using Novus' PBP Antibody

I first tried the PBP antibody (NB110-93495) in June of 2010 and it worked well. I picked this antibody because it had been tested in rat tissue, so I was confident it would work for my rat samples. I stored the PBP antibody in 20ul aliquots after it arrived then stored it at -20C and used it over a 3 month period. I ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from the S1 proximal tubule segment.

A Multicolored Approach to CD4 Antibody Assays

Novus Biologicals offers the widest selection of conjugated CD4 antibody products, with a database that's continually expanding to take into account the latest developments. This includes CD4 antibodies conjugated to Dynomics and PE fluorescent dyes, for FACS analysis.

Beta Actin and GAPDH: The Importance of Loading Controls

Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control antibody helps determine if samples have been loaded equally across all wells and confirms effective protein transfer during the western blot protocol.

Epitope Tagging: From His to OLLAS

Epitope tagging is a procedure that inserts a short amino acid sequence into a protein within an expression vector via genetic engineering. Antibodies that recognize the tag can then be used to detect the protein when no antibody to the target protein exists or when the target protein shows low immunogenicity.  Such tags can be inserted at the C-terminus, N-terminus or even within the protein itself.  Terminal insertion is preferred as internal tags may alter protein function or become buried within the tertiary structure of the protein.

The GAPDH Antibody in Western blot Assays

The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for checking the even loading of gel samples, and the even transfer of proteins at the blotting stage. They also serve a purpose in quality control, by verifying reagents are working correctly, and in the standardization of experimental results.

New Primers Available for ChIP Procedures

Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins.

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