HAF008). Specific bands were detected for V5 Epitope-tagged at approximately 110 kDa, 34 kDa, 75 kDa, and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal V5-tagged CBL, N-terminal V5-tagged FSNC4, C-terminal V5-tagged LAMP2B, and C-terminal V5-tagged GALNT2. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-V5 Epitope Tag Monoclonal Antibody (Catalog # MAB8926) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for V5 Epitope-tagged at approximately 110 kDa, 34 kDa, 75 kDa, and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB8926G-100UG | |||
| Carrier Free | FAB8926V-100UG | |||
| Carrier Free | FAB8926U-100UG | |||
| Carrier Free | FAB8926T-100UG | |||
| Carrier Free | FAB8926S-100UG | |||
| Carrier Free | FAB8926R-100UG | |||
| Carrier Free | FAB8926N-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81035-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52653-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52703-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81036-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81037-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37825-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80562-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - ATG2A-V5 overexpression reduces intracellular neutral lipids in THP-1 cells. Scale bars, 10 um. Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection. <i>Nat Commun</i> (2017)](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-381-img0009.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/mL (1:25,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0002.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 ug/mL (1:5000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0001.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-381] - Analysis using the HRP conjugate of NB600-381. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/mL (1:5,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-381-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-381-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody [NB600-381] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunohistochemistry-NB600-381-img0008.jpg)
![Immunoprecipitation: V5 Epitope Tag Antibody [NB600-381] - HEK293T were transfected with V5-KU86 plasmid, followed by V5 antibody IP then Western blot with v5 antibody (NB600-381). Primary antibody at 1:1000, 4C, overnight. IP/WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Immunoprecipitation-NB600-381-img0012.jpg)
![Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - (a) Light & fluorescent micrographs of stable HN33.11 cells expressing (A)-GFP or (B)-GFP, respectively. Results from two stable clones of each transfection pool are shown. Cells are treated with vehicle (Dox−) or 1 μg/mL Dox (Dox+). (b) Western blot of homogenates of stable clones showing endogenous production of full-length p75NTR (FLp75), Dox-induced, but “leaky” production of (A) & (B) & of the V5 tag. Control values for each clone do not differ significantly between GFP(−) & GFP(+) cells (Student's t-test). ***P < 0.001 relative to GFP(−) cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21904642), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-381_rabbit-polyclonal-v5-epitope-tag-antibody-31020241539253.jpg)
![Western Blot: V5 Epitope Tag Antibody - BSA Free [NB600-381] - Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alpha A. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-381_rabbit-polyclonal-v5-epitope-tag-antibody-310202415304231.jpg)
![Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-380-img0005.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-380] - Detection of V5-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody NB600-380 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: V5 Epitope Tag Antibody [NB600-379] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-379-img0001.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-379] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")
AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # 
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunocytochemistry-Immunofluorescence-NBP2-43626-img0010.jpg "Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunoprecipitation-NBP2-43626-img0002.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] (NBP2-43626) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43626_mouse-monoclonal-v5-epitope-tag-antibody-1071-western-blot-2611202518493923.jpg)
![Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0003.jpg "Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 15ug of 293T cell lysates, from cells transfected with a V5-Wnt3a or V5-Vstm2 construct for the indicated number of hours, per well. Data courtesy of the Innovators Program.")
![Western Blot: V5 Epitope Tag Antibody (E10/V4RR) [NBP2-37825] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-E10-V4RR-Western-Blot-NBP2-37825-img0004.jpg)
![Western Blot: V5 Epitope Tag Antibody (SV5-Pk5) [NBP1-80562] - V5 tagged protein detected with Mouse anti V5-tag.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-SV5-Pk5-Western-Blot-NBP1-80562-img0001.jpg "Western Blot: V5 Epitope Tag Antibody (SV5-Pk5) [NBP1-80562] - V5 tagged protein detected with Mouse anti V5-tag.")
