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NL001) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
1065-AP) was serially diluted and captured by Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB10651) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB1065) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
![Immunocytochemistry Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Adiponectin_AF1119_Immunocytochemistry_11826.jpg)
![Western Blot Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Adiponectin_AF1065_Western_Blot_16816.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Adiponectin_DRP300_ELISA_812.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Adiponectin_MRP300_ELISA_1026.jpg)
![N/A Adiponectin/Acrp30 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Adiponectin_DY1065_ELISA_1560.jpg)
![Immunocytochemistry Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Adiponectin_AF1119_Immunocytochemistry_11827.jpg)
![Western Blot Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1119_mouse-adiponectin-acrp30-affinity-purified-polyclonal-ab-8220249511422.jpg)
![Western Blot Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1119_mouse-adiponectin-acrp30-affinity-purified-polyclonal-ab-western-blot-12122025932370.jpg)
![Simple Western Adiponectin/Acrp30 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/Adiponectin_AF1065_Simple_Western_18670.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Adiponectin_DRP300_ELISA_813.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/elisa/Adiponectin_DRP300_ELISA_173.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/elisa/Adiponectin_MRP300_ELISA_316.jpg)
![N/A Adiponectin/Acrp30 [HRP]](http://images.novusbio.com/fullsize/mrp300_mouse-adiponectin-acrp30-quantikine-elisa-kit-44202415425058.jpg)
