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493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse VEGF164(5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471). The ND50 is typically 2-8 µg/mL." class="big_lightbox" target="_blank">
471-F1) was serially diluted and captured by Goat Anti-Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
471-F1) was serially diluted and captured by Goat Anti-Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF471) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.