Western Blot: CHD3 Antibody (2G4) [NBP1-51593] - Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
Immunocytochemistry/ Immunofluorescence: CHD3 Antibody (2G4) [NBP1-51593] - Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: CHD3 Antibody (2G4) [NBP1-51593] - CHD3 is expressed in all neuronal cell types of the hippocampus. Mouse brain sections were immunostained with antibodies against CHD3 (red), Satb2 or GABA ...read more
Flow Cytometry: CHD3 Antibody (2G4) [NBP1-51593] - Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: CHD3 Antibody (2G4) [NBP1-51593] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CHD3 mouse mAb with DAB staining.
Immunohistochemistry: CHD3 Antibody (2G4) [NBP1-51593] - CHD3 is expressed in all neuronal cell types of the hippocampus. Mouse brain sections were immunostained with antibodies against CHD3 (red), Satb2 or GABA ...read more
Immunohistochemistry: CHD3 Antibody (2G4) [NBP1-51593] - CHD3 is expressed in all neuronal cell types of the hippocampus. Mouse brain sections were immunostained with antibodies against CHD3 (red), Satb2 or GABA ...read more
226 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified
Alternate Names for CHD3 Antibody (2G4)
ATP-dependent helicase CHD3
CHD-3
chromodomain helicase DNA binding protein 3
chromodomain-helicase-DNA-binding protein 3
EC 3.6.1
EC 3.6.4.12
hZFH
Mi-2 autoantigen 240 kDa protein
Mi-2a
Mi2-ALPHA
ZFH
Zinc finger helicase
zinc-finger helicase (Snf2-like)
Background
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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