Product Search for caspase 1: Novus Biologicals

AF835

Caspase-3 Antibody [Unconjugated] - Active

(7 Reviews)

50 μg

AF835

Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for <a class=

Fluorescent ICC Staining of Cells on Coverslips." alt="Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Active Caspase-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Representative sections of the medial side of right tibial epiphysis stained with/without caspase-3. Sections stained with caspase-3 antibody at four different time points (DH: A, C, E and G; BS2: B, D, F and H); a higher magnification (I); the growth plate (J) and fibrocartilage of anterior cruciate ligament (K) show caspase-3 positive cells (red cytoplasmic staining; arrow). A negative control section is shown in (L). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/14/9/17729), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Immunohistochemical staining for caspase 3 on surgically transected tumours. A) Epo-treated tumour 24 hours after surgery. B) Placebo-treated tumour 24 hours after surgery. C) Placebo-treated tumour 48 hours after surgery Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-648), licensed under a CC-BY license. Not internally tested by R&D Systems.

LPS challenge induces cell shedding from small intestinal villi. C57 BL/6 mice were administered either (a) PBS (control) or (b) LPS by IP injection and proximal small intestines removed after 1.5 h. Processed tissue was sectioned and stained by immunohistochemistry for CC3 (i.e. brown cells indicate shedding event), also highlighted by arrows. A representative picture for each group is shown (12 mice per group, two independent experiments). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

The cytoprotective effect of B. breve is MyD88 dependent. (a,b) IEC MyD88+/+ mice and (c,d) IEC MyD88−/− mice were gavaged with PBS (control) or B. breve UCC2003 and challenged with LPS. Paraffin-embedded intestinal sections were stained with anti-CC3 and quantified using the WinCrypts and Score programs. (e) Columns shown TLR2 expression via RT-PCR. Data are mean ± s.d., n = 12 (two independent experiments) analysed with Mann–Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

Bifidobacterium breve UCC2003 protects against LPS-induced cell shedding. C57 BL/6 mice received three daily oral gavage doses of (a) PBS or (b) approximately 1 × 109B. breve UCC2003 followed by IP challenge with LPS 24 h later. Representative images are shown. Formalin-fixed, paraffin-embedded intestinal sections were sectioned and stained with anti-CC3 and (c) quantified using the WinCrypts and Score programs, 20 well-orientated hemi-villi were counted per mouse. Data are mean ± s.d., n = 12 (two independent experiments) analysed with a Mann–Whitney U-test. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28123052), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Genetic deletion of Bax prevents mutant LRRK2 induced neuronal death. Primary embryonic cortical neurons are derived from WT mice or mice lacking the pro-apoptotic protein Bax, and transiently transfected with GFP-tagged WT or mutant LRRK2 (R1441C, G2019S). (a) A representative image showing typical morphological features of apoptotic neurons expressing mutant LRRK2; that are positive for active caspase-3 (casp3) and display condensed and fragmented nuclei. (b) Quantification of apoptotic neuronal death (apoptotic nuclear profiles, “apo”; and active caspase-3 positive neurons, “casp3”) from cultures depicted in (a); **p < 0.01 in comparison to WT transfected neurons. (c) Primary neurons were prepared from WT mice or mice deficient in Bax, and treated with the topoisomerase I inhibitor camptothecin (“cam”, 5 μM, 18 hr), or doxorubicin (“doxo”, 10 ng/ml, 36 hr). Cells were fixed and processed for apoptotic nuclear counts. (d) Primary cortical neurons were prepared from WT mice or mice deficient in Bax, and transiently transfected with WT, R1441C, or G2019S LRRK2. As before, the cultures were fixed and the percentage of LRRK2-positive neurons that displayed apoptotic nuclear features was determined. The absence of Bax significantly protected neurons from death induced by mutant LRRK2. ***p < 0.001 compared to WT neurons expressing R1441C- or G2019S-LRRK2. Each data point represents the mean +/− SEM from 3–4 independent transfections from a representative culture. Cultures were repeated at least 3 times with similar differences. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29472595), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hepatocyte ARNT deletion alters fibrotic and apoptotic gene expression in the TAA model of fibrosis.Results for FC are shown in black columns and LARNT in white columns. Results are expressed relative to FC level. (A) Messenger RNA expression of ARNT and collagen isotypes. (B) Expression of genes regulating fibrosis in FC and LARNT mice. (C) F4/80 and inflammatory cytokine expression in FC and LARNT mice. (D) Apoptotic gene expression in FC and LARNT mice. (E) Representative histology of FC liver stained with anti-Caspase 3 at 100X magnification. (F) Representative histology of LARNT liver stained with anti-Caspase 3 at 100X magnification. (G) Average Caspase 3 positive cell counts per field of view at 100 X magnification. Mean±SEM, * = p<0.05 ** = p<0.01, *** = p<0.001. N = 5-6/group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0121650), licensed under a CC-BY license. Not internally tested by R&D Systems.

p140Cap expression is causative in limiting tumour growth in NeuT mice.(a) Expression cassette used for the generation of MMTV-p140Cap transgenic mice. The Myc epitope is inserted at the carboxyterminal region of the protein. (b) Extracts of tumours derived from NeuT mice (a,b) or p140-NeuT mice (c,d) were run on 6% SDS–PAGE and stained with antibodies to p140Cap, ERBB2 and tubulin for loading control. (c) Percentage of tumour free mice in NeuT (red line) and p140-NeuT (blue-dashed line) transgenic animals in both FVB (left) or BALB/c background (right). Twelve mice were analysed for each group. Fisher's exact test, Two sided, P=0,0022; P=0,0056. Error bar: s.e.m. (d) Total tumour burden in NeuT (red) and p140-NeuT (blue) mice in both the FVB (left) or BALB/c backgrounds (right) was measured. Ten mice were analysed in each group. Unpaired t test: (*P<0.05; ***P<0.001). Error bar: s.e.m. (e) Paraffin-embedded sections from three NeuT and three p140-NeuT tumours taken from mice at 33 weeks of age were analysed for hematoxylin–eosin (H&E) (a–d) and for immunohistochemistry with antibodies to NeuT (e–h), PCNA (i–l) and activated Caspase3 (m–p). Representative images are shown. Scale bar, 50 μm (first and third columns); 20μm (second and fourth columns). Histograms on the right show the percentage of PCNA+ (upper panel) and Activated Caspase3+ (lower panel) cells. Statistical significative differences were evaluated using unpaired t-tests (***P<0.001). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14797), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Microglia-derived TNF-alpha alleviates aberrant neurogenesis.(a) qRT-PCR analyses of Tnf-alpha levels in the DG of WT and TLR9 KO mice at the indicated time points after seizure. Experimental controls were KA-untreated WT and TLR9 KO mice, respectively (n=3 animals). *P<0.05 by analysis of variance (ANOVA) with Tukey post-hoc tests. (b) Experimental scheme for assessing aNS/PC proliferation in microglia culture-derived CM pretreated with TNF-alpha -neutralizing antibody or IgG control. (c) Representative images of EdU (red), active caspase3 (green) and Hoechst (blue) staining in aNS/PCs cultured with the indicated CM from microglia (n=4 experiments). Scale bar, 50 μm. (d) Quantification of EdU+ or active caspase3+ cells in c (n=4 experiments). (e) Experimental timeline for assessing aNS/PC proliferation in thalidomide (Thal)-treated mice. (f) Representative images of BrdU+DCX+ newly generated immature neurons in the DG (n=4 animals). Scale bar, 50 μm. (g,h) Quantification of the number of BrdU+DCX+ cells in f, in the DG (g) and in the hilus (h) (n=4 animals). *P<0.05 and **P<0.01 by ANOVA with Tukey post-hoc tests. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25751136), licensed under a CC-BY license. Not internally tested by R&D Systems.

Double immunofluorescent staining of FSGS kidneys to Ki-67, alpha -tubulin and DAPI or double immunofluorescent staining to caspase-3 and DAPI. A. FSGS kidneys: cysts with simple squamous epithelium (CS) filled with colloid content, interstitium (i) and collecting tubules (ct) contain Ki-67-positive cells (arrows)(square). B. alpha -tubulin visualizes short cilia (arrows) in the cysts and extremely long cilia in collecting tubules (arrows)(square). C. Nuclear DAPI stain. D. Merging of A + B + C shows relationships between proliferating cells (green) and cilia (red). Immunostaining to Ki-67, alpha -tubulin and DAPI; scale bar 10 μm. E. Distribution of Ki-67 positive cells (%) in CNF and FSGS nephrotic syndrome. Cysts of proximal tubules with simple squamous epithelium (CS) or simple cuboidal epithelium (CC), proximal tubules with apparently normal simple cuboidal/columnar epithelium (PTNC). Data are shown as mean ± SD. Significant differences (Kruskal–Wallis) indicated by ***p < 0.0001. F. FSGS kidneys: caspase-3-positive apoptotic cells (arrows) in proximal tubules (PTNC) and interstitium (i). G. Nuclear DAPI stain. H. Merging of F + G shows green caspase-3- positive apoptotic nuclei and blue non-apoptotic nuclei. Immunostaining to caspase-3 and DAPI, scale bar 10 μm. Image collected and cropped by CiteAb from the following publication (//bmcnephrol.biomedcentral.com/articles/10.1186/1471-2369-15-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Epidermis-specific deletion of Rac1 increases UV-light-induced keratinocyte apoptosis in vivo. (a) H/E staining of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Black arrows indicate sunburn cells. (b) Graph shows the percentage number of sunburn cells at 12 h with (red bars) or without (blue bars) UV-irradiation in Rac1 fl/fl (n=4) and Rac1-EKO (n=5) mice. The percentage of sunburn cells within the epidermis after UV-irradiation in Rac1 fl/fl mice was 3.4%, whereas in Rac1-EKO mice it was 8.6% of total epidermal keratinocytes. Non-irradiated samples showed <1% sunburn cells in both the genotypes. (c) Immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Nuclei are stained in blue. Scale bar=100 μm. (d) Western blot analysis of cleaved caspase-3 from epidermal lysates of untreated (no UV) and UV-light treated (UV) Rac1 fl/fl and Rac1-EKO mice. Non-irradiated controls showed no bands for cleaved capsase-3, whereas samples of irradiated epidermis showed cleaved caspase-3-specific bands at ~25 kDa and 23 kDa. Numbers on the left denote molecular weights in kDa. (e) Graph shows densitometry analysis of western blot in (d). Error bars show S.D. Asterisks show P-value<0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

DNA damage has an important role in increased UV-light-induced keratinocyte apoptosis in Rac1-EKO epidermis in vivo. (a and c) H/E stainings and immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of CPDPL/Rac1-EKO mice kept in the dark and under the photoreactivation lamp. Nuclei are stained in blue. Dashed line in c demarcates border between epidermis and dermis. Scale bar=100 μm. (e) Western blot analysis of cleaved caspase-3 from epidermal lysates of UV-irradiated CPDPL/Rac1-EKO mice kept in the dark and under the photoreactivation lamp. GAPDH was used as a loading control. Numbers on the left denote molecular weight in kDa. (b,d, and f) Graphs show quantification of sunburn cells (n=5, 4) (b), cleaved caspase-3 positive cells (n=9, 8) (d) and densitometric analysis of cleaved caspase-3 western blots (n=4, 6) (f) from UV-irradiated CPDPL/Rac1-EKO mice kept in the dark (black bars) and under the photoreactivation (PR) lamp (gray bars). Error bars show S.D. Asterisks show P-value. **<0.01, ***<0.001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Smad1 deletion reduces oxidative stress and apoptosis following stroke.(A) Fluorescent images and quantification of in vivo HEt assay reflecting ROS levels on the ipsilateral hemisphere at 7 days post-stroke. The extent of oxidized nucleotides was measured by IHC for 8-oxo-dG levels at 24 h post-stroke. (B) IHC and quantification of activated Caspase 3 (aCasp3) and p53 on ipsilateral hemisphere at 7 days post-stroke. n = 3 for HEt, 8-oxo-dG, aCasp3, n = 4 for p53, unpaired Student’s t-test, *p<0.05; ***p<0.001. Scale, 50 μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0136967), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on IL-1 beta -induced NF-kappa B-dependent expression of pro-inflammatory, pro-apoptotic and matrix degrading gene products, primary chondrocytes were either stimulated with 10 ng/ml IL-1 beta , 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) or pre-stimulated for 12 h with 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) followed by 10 ng/ml IL-1 beta for 24. Equal amounts of total proteins were separated by SDS-PAGE and analyzed by immunoblotting using antibodies raised against COX-2, MMP-9 and MMP-13 and active caspase-3. Stimulation with IL-1 beta resulted in production of COX-2, MMP-9, MMP-13 and caspase-3 cleavage. Pre-treatment with a combination of both IGF-1 or/and PDGF-bb downregulated COX-2, MMP-9, MMP-13 and cleaved caspase-3. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0028663), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of IGF-1 or/and PDGF-bb on IL-1 beta -induced NF-kappa B-dependent pro-inflammatory, pro-apoptotic and matrix degrading gene products in chondrocytes.To determine whether IGF-1 or/and PDGF-bbexert effects on IL-1 beta -induced NF-kappa B-dependent expression of pro-inflammatory, pro-apoptotic and matrix degrading gene products, primary chondrocytes were either stimulated with 10 ng/ml IL-1 beta , 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) or pre-stimulated for 12 h with 10 ng/ml PDGF-bb, 10 ng/ml IGF-1 or combination of both growth factors (5 ng/ml each) followed by 10 ng/ml IL-1 beta for 24. Equal amounts of total proteins were separated by SDS-PAGE and analyzed by immunoblotting using antibodies raised against COX-2, MMP-9 and MMP-13 and active caspase-3. Stimulation with IL-1 beta resulted in production of COX-2, MMP-9, MMP-13 and caspase-3 cleavage. Pre-treatment with a combination of both IGF-1 or/and PDGF-bb downregulated COX-2, MMP-9, MMP-13 and cleaved caspase-3. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22194879), licensed under a CC-BY license. Not internally tested by R&D Systems.

Allelic loss of Cdh11 led to faster growing tumors due to decreased cell death. (E) Cadherin-11 was knocked down using stealth siRNA in a cadherin-11 expressing cell line derived from TAg-RB tumors, T +539. Knockdown of Cdh11 clearly decreased caspase-3 expression compared to control. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20421947), licensed under a CC-BY license. Not internally tested by R&D Systems.

Curcumin inhibits IL-1 beta activity, enabling co-culture induced chondrogenesis in MSCs. A: Fourteen days high-density culture. Untreated MSC cultures became apoptotic (a). In primary chondrocyte cultures (b), co-cultures (c), co-cultures treated with curcumin (e) or co-cultures pre-stimulated four hours with curcumin (f), prominent chondrogenesis was observed. Stimulation of the co-culture with IL-1 beta alone resulted in degeneration of the cell culture (d). In contrast, a four-hour pre-stimulation of the co-culture with curcumin followed by IL-1 beta incubation (g) or a four-hour pre-stimulation of the co-culture with curcumin followed by IL-1 beta and curcumin incubation (h) inhibited the adverse effects of IL-1 beta on the chondrogenic potential of the co-culture and prominent chondrogenesis was observed. Magnification, 6,000×; bar, 1 μm; C, chondrocytes, F, fibroblast-like cells; M, ECM. B-D: Immunoblots of whole cell lysates were probed with antibodies against CSPGs, collagen type II, beta 1-integrin, Shc, activated-ERK1/2, Sox-9, activated-caspase-3 and COX-2. In co-cultures pre-stimulated for four hours with curcumin followed either by incubation with IL-1 beta alone or incubation with IL-1 beta and curcumin, prominent production of chondrogenic matrix and adhesion molecules (B), activation of the chondrogenic signalling pathway (C) and down-regulation of apoptotic and inflammatory markers (D) was observed. Each experiment was performed in triplicate. Expression of the housekeeping gene beta -actin was not affected. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/20594343), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Reactivity: Hu, Mu

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Host: Rabbit Polyclonal

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NB100-56708

Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free

(2 Reviews)

(202 Publications)

0.1 mg

NB100-56708

Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.

<b>Biological Strategies Validation. </b>Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.

Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 1:50 dilution of mouse aCaspase-3 Antibody (31A1067) - (Pro and Active) (NB100-56708), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer(VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).

Caspase-3-Antibody-31A1067-Pro-and-Active-Western-Blot-NB100-56708-img0044.jpg

An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.

<b>Biological Strategies Validation. </b>Lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with VP-16. PVDF membrane was probed with 0.1 ug/mL of mouse monoclonal Caspase-3 Antibody (31A1067) - (Pro and Active) (NB100-56708, Novus Biologicals) followed by 1:2000 dilution donkey anti-mouse IgG.

An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Western Blot: Mouse Monoclonal Caspase-3 Antibody (31A1067) - (Pro and Active) [IMGENEX: IMG-144A] [NB100-56708] - Whole cell lysates from MDA-MB-231, MCF-7, SUM149 and SUM159 cells were loaded with 50 ug/lane. 10% SDS-PAGE. Caspase-3 Antibody (NB100-56708) was used for primary antibody: 1:2000, 4℃, overnight. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - rOPN administration influenced the interaction & balance between Beclin 1 & Caspase‐3 at 24 h after SAH. Double immunofluorescence staining of Caspase‐3 & Beclin 1 in Sham group, SAH + Vehicle group & SAH + rOPN group at 24 h after SAH induction. Sample size is 9, n = 3 per group. Localization of Caspase‐3 can be cytoplasmic & nuclear. Staining in the nucleus is considered to be an indication of active Caspase‐3. The dashed lines & the red box on brain slice images indicate the locations observed. Vehicle, phosphate‐buffered saline; Scale bar = 50 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - PEM induces YIPF2 upregulation & apoptosis in NSCLC cells.a, b H1792 (a) & H1299 (b) NSCLC cells were treated with PEM at various concentrations (0–10.0 μM) for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2 & ACTB. c Overexpression of YIPF2 in H1299 cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, CASP8, CASP3, PARP1 & ACTB. d Knockdown of YIPF2 expression by YIPF2–1 siRNA in A549 NSCLC cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, CASP8, CASP3, PARP1 & ACTB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32303681), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - Lipofection of top broccoletti-miR candidates does not influence basal & induced apoptosis.(A) BxPc-3 & Bx-Gem cells were transfected as described in Figure 3. Seventy-two hours later, the cells were stained with Annexin V-PE & 7-AAD, followed by FACS analysis. The percentage of Annexin V- & 7-AAD-positive cells is shown. (B) Lipofected BxPc-3 & Bx-Gem cells were stained with an antibody specific for the proliferation marker Ki-67 (green) or the apoptosis marker cleaved fragment of caspase-3 (red), which indicates apoptosis. Representative images at ×100 magnification are shown. The percentage of Ki-67- or caspase-3-positive cells was counted in 18 visual fields, & the means ± SD are shown in the diagram below. (C) BxPc-3 & Bx-Gem were lipofected as described above, & at 24 h later, the cells were treated with gemcitabine (10 nM) or were left untreated. Ninety-six hours after gemcitabine treatment, viability was determined by MTT assay. The data are presented as the means ± SD (**P < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32292571), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - PEM induces apoptosis of NSCLC cells via YIPF2-TNFRSF10B axis.a, b A549 (a) & H1792 (b) cells were treated with PEM at 5.0 μM for the indicated times (0, 6, 12, 24, 36 & 48 h). Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B & ACTB. c A549 cells were treated with doxorubicin (DOX) at various concentrations (0–2.0 μM) for 18 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B & ACTB. d Overexpression of YIPF2 in H1792 & H1299 cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B & ACTB. e Knockdown of YIPF2 expression by YIPF2–1 siRNA in A549 & H1792 cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B & ACTB. f Overexpression of YIPF2 in H1299 cells (left) or Knockdown of YIPF2 expression by YIPF2–1 siRNA in H1792 cells (right) in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10A & ACTB. g Three A549 cell lines (Ctrl, YIPF2, YIPF2 + siTNFRSF10B) in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B, CASP8, CASP3 & ACTB. h Three H1299 cell lines (Ctrl, siYIPF2–1, siYIPF2–1 + TNFRSF10B (short isoform)) in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against YIPF2, TNFRSF10B, CASP8, CASP3 & ACTB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32303681), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - Cell numbers positively stained for NeuN, DCX, PCNA & caspase-3 in the subgranular region of the dentate gyrus in WT & RanBP9−/− (KO) mice.(A), DAPI-stained brain sections to show the highlighted subgranular zone within the dentate gyrus region of the hippocampus used for cell counts shown in B. (B), Representative brain sections stained with anti-NeuN, anti-DCX, anti-PCNA, anti-capsase-3 & counter stained with DAPI. Cell counting revealed significantly decreased numbers of NeuN positive cells in RanBP9−/− (KO) brains (22%) compared to WT controls. However DCX, PCNA & caspase positive cell numbers were not significantly altered. In each group, n = 3, data presented as mean± SEM. **, p<0.01 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23840553), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - NFATc1 regulates DcR3 expression at a transcriptional level. (A,B) Immunoblot analysis of whole-cell lysates & quantitative real-time-PCR assaying relative DcR3 mRNA expression of ACHN & 769-P cells 24 h after treatment with cyclosporin A (CsA, 25 μM) or Tacrolimus (FK-506, 50 μM) (A); 48 h post transfection with NFATc1 or an empty vector control (neo) (B). Expression data were normalized to internal 18S rRNA expression (mean ± SEM; n=3; *p<0.05, **p<0.01, ***p<0.001; T-test). (C) Immunoblot analysis of cytoplasmic & nuclear fractions of ACHN & 769-P cells after treatment with LY294002 (50 μM), Everolimus (1 μM), or Cyclosporine A (25 μM). (D) Relative NFATc1–luciferase reporter activity of ACHN & 769-P cells 24 h post transfection with myrAkt or an empty vector control (neo) (mean ± SEM; n=3; *p<0.05, **p<0.01; T-test). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24107265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - rOPN administration elevated the expression of autophagy‐related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of (A) Beclin 1, mean ± SD is 1.016 ± 0.2262 in Sham group, 2.874 ± 1.147 in SAH + Vehicle group, 4.963 ± 2.05 in SAH + rOPN group, F = 15.52, (B) ATG5, mean ± SD is 0.8908 ± 0.2545 in Sham group, 2.332 ± 0.6431 in SAH + Vehicle group, 4.364 ± 1.309 in SAH + rOPN group, F = 25.02, (C) LC3, mean ± SD is 1 ± 0.1845 in Sham group, 1.755 ± 0.3017 in SAH + Vehicle group, 2.7 ± 0.7957 in SAH + rOPN group, F = 17.23, (D) Cleaved Caspase‐3, mean ± SD is 1.008 ± 0.186 in Sham group, 4.112 ± 1.528 in SAH + Vehicle group, 2.291 ± 0.6268 in SAH + rOPN group, F = 15.86, (E) Bax, mean ± SD is 1.006 ± 0.321 in Sham group, 37.47 ± 10.86 in SAH + Vehicle group, 23.83 ± 8.143 in SAH + rOPN group, F = 33.13, (F) Bcl‐2, mean ± SD is 1.005 ± 0.2736 in Sham group, 0.2309 ± 0.1257 in SAH + Vehicle group, 0.7843 ± 0.2278 in SAH + rOPN group, F = 20.1, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean ± SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31436915), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free [NB100-56708] - RAB8 suppresses PEM-induced apoptosis of NSCLC cells by promoting the removing of TNFRSF10B from plasma membrane to cytoplasm.a, b Knockdown of RAB8 expression by RAB8 siRNA in H1792 (a) & A549 (b) cells in the presence or absence of PEM at 5.0 μM for 36 h. The surface expression of TNFRSF10B was confirmed by flow cytometry analyses. c Overexpression of RAB8 in H1299 cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against RAB8, TNFRSF10B, CASP8, CASP3, PARP1 & ACTB. d Knockdown of RAB8 expression by RAB8 siRNA in H1792 cells in the presence or absence of PEM at 5.0 μM for 36 h. Cell lysates were analyzed by Western blotting with antibodies against RAB8, TNFRSF10B, CASP8, CASP3, PARP1 & ACTB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32303681), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Ch, ChHa, Ma

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Ch, ChHa, Ma

Applications:

, , , , , , , , , , ,

Applications: , , , , , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-144A

Alt Catalog# IMG-144A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56708AF350
Alexa Fluor 405	NB100-56708AF405
Alexa Fluor 488	NB100-56708AF488
Alexa Fluor 532	NB100-56708AF532
Alexa Fluor 594	NB100-56708AF594
Alexa Fluor 647	NB100-56708AF647
Alexa Fluor 700	NB100-56708AF700
Alexa Fluor 750	NB100-56708AF750
Alexa Fluor Plus 405	NB100-56708AFP405
Alexa Fluor Plus 488	NB100-56708AFP488
Alexa Fluor Plus 555	NB100-56708AFP555
Alexa Fluor Plus 594	NB100-56708AFP594
Alexa Fluor Plus 647	NB100-56708AFP647
Alexa Fluor Plus 680	NB100-56708AFP680
Allophycocyanin	NB100-56708APC
Biotin	NB100-56708B
CoraFluor 1	NB100-56708CL1
DyLight 350	NB100-56708UV
DyLight 405	NB100-56708V
DyLight 488	NB100-56708G
DyLight 550	NB100-56708R
DyLight 594	NB100-56708DL594
DyLight 650	NB100-56708C
DyLight 680	NB100-56708FR
DyLight 755	NB100-56708IR
FITC	NB100-56708F
HRP	NB100-56708H
Janelia Fluor 525	NB100-56708JF525
Janelia Fluor 549	NB100-56708JF549
Janelia Fluor 646	NB100-56708JF646
Janelia Fluor 669	NB100-56708JF669
PE	NB100-56708PE
PerCP	NB100-56708PCP
mFluor Violet 450 SE	NB100-56708MFV450
mFluor Violet 500 SE	NB100-56708MFV500
mFluor Violet 610 SE	NB100-56708MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56708SS
Azide and BSA Free	NBP2-33244

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(select up to 3 products from this page to compare)

NB100-56565

Caspase-1 Antibody (14F468) - BSA Free

(73 Publications)

0.1 mg

NB100-56565

Simple Western lane view shows a specific band for Caspase 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0015.jpg

Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0017.jpg

<b>Biological Strategies Validation. </b>Caspase-1-Antibody-14F468-Immunohistochemistry-NB100-56565-img0020.jpg

Caspase-1-Antibody-14F468-Western-Blot-NB100-56565-img0016.jpg

Analysis of Caspase-1 using a Caspase-1 monoclonal antibody. Human HeLa (A) and mouse NIH3T3 lysate probed with Caspase-1 antibody at 0.5 ug/ml and 2 ug/ml, respectively.

Adenocarcinoma of the rectum stained with Caspase-1 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Detection of Caspase-1 protein in a section of normal human colon using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Distinct cytoplasmic staining along with some nuclear positivity was observed in crypts/mucosa, and staining was found to be more intense in the absorptive columnar epithelial cells. [10X Magnification]

Normal lung from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). In this representative lung section, different type of cells including pseudostratified columnar epithelium of bronchiole and the simple squamous epithelium of alveoli may be seen to develop immunoreactivity for Caspase 1. [10X Magnification]

Normal skin from human using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468). Strong cytoplasmic/nuclear staining developed in all the epidermal cells, blood vessels and some cells of the dermal connective tissues layer. [10X Magnification]

Detection of Caspase-1 in a section of human ovarian cancer using 5 ug/ml concentration of Caspase 1 antibody (clone 14F468).

Tissue section of human intestine using Caspase-1 antibody (clone 14F468) at 5ug/ml concentration (1:200 dilution). The primary antibody binding to Caspase 1 in cells was detected using HRP conjugated anti-Mouse secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This Caspase 1 antibody generated a diffused but specific cytoplasmic staining in columnar epithelia cells of villi, and a few cells depicted nuclear staining also. Only a subset of connective tissue cells in lamina propria depicted positivity (cytoplasmic) for this protein.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Glucose fluctuations promoted the activation of NLRP3 inflammasome. (A) Immunofluorescence staining of NLRP3-ASC interaction in rat hearts. The heart sections were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (B–D) The mRNA levels of NLRP3, IL-1 beta & IL-18 in rat hearts of the three groups (n = 4 per group). (E–H) The protein expressions of NLRP3, ASC & cleaved caspase-1 in rat hearts of the three groups (n = 4 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Caspase-1 expression increases with age in human fibroblasts: a Immunoblot analysis of caspase-1 & ASC in human fibroblasts obtained from a donor at the ages of 49, 52 & 64 y/o. b Immunocytochemistry image of human fibroblasts stained for caspase-1 (green) & Phalloidin (red) from a donor at three different ages (49, 52 & 64 y/o). Bar graph: 100 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Aging alters expression of NLRP1 inflammasome components. Representative immunoblots & densitometric analysis of immunoblots for caspase-1 (Casp1), caspase-11 (Casp11), NLRP1, ASC, cleaved XIAP, P2X7 receptor (P2X7R) & pannexin-1 (PanX1) in brain lysates of young (Y) & aged (A) animals. Protein levels of cleaved caspase-1 & -11 are higher in aged animals compared to young. Protein levels of NLRP1 & ASC did not change with age whereas P2X7 receptor, the pannexin-1 protein & the cleaved fragment of XIAP are higher in aged animals than in young animals. beta -Tubulin was used as an internal standard & control for protein loading. Data are presented as mean +/- SEM. *p < 0.05, **p < 0.01, ***p < 0.005 compared with young. n = 6 per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22133203), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Role of NF-kappa B in glucose fluctuation-induced inflammasome-related myocardial fibrosis. (A) Immunofluorescence staining of NF-kappa B/p65 nucleation (n = 3 per group). (B,C) The phosphorylation level of NF-kappa B/p65 in rat hearts of the three groups (n = 4 per group). (D) Immunofluorescence staining of intracellular NLRP3–ASC interaction in the GF & GF + TPCA-1 groups. The NRCFs were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (E–G) After application of TPCA-1 (0.5 μM), the protein expressions of cleaved caspase-1 & collagen I were measured by western blot (n = 3 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Inflammasome proteins are elevated in the cytosolic fraction of aged mice: a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the cortex of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (b), caspase-1 (c), ASC (d), IL-18 (e) in the cortex. a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the hippocampus of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (g), caspase-1 (h), caspase-11 (i), ASC (j), IL-1 beta (k) in the hippocampus. Data presented as mean+/-SEM. N = 5 per group. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - NLRP3 inhibition with MCC950 reversed myocardial fibrosis induced by glucose fluctuations. (A) Representative images of hematoxylin & eosin (HE) staining for myocardial tissue in the FBG + NaCl & FBG + MCC950 groups (n = 3 per group). (B–C) Representative images of myocardial fibrosis & fibrosis area (%) in the FBG + NaCl & FBG + MCC950 groups (n = 4 per group). (D–K) The protein expressions of NLRP3, cleaved caspase-1, TGF-beta 1, collagen I & collagen III in the FBG + NaCl & FBG + MCC950 groups (n = 6 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Inflammasome proteins are elevated in the cytosolic fraction of aged mice: a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the cortex of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (b), caspase-1 (c), ASC (d), IL-18 (e) in the cortex. a Representative image of immunoblot analyses of inflammasome proteins in the cytosolic fraction of the hippocampus of young (Y) & aged (A) mice. Quantification of immunoblot analysis of NLRC4 (g), caspase-1 (h), caspase-11 (i), ASC (j), IL-1 beta (k) in the hippocampus. Data presented as mean+/-SEM. N = 5 per group. *p < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30473634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Role of inflammasome in glucose fluctuation-induced myocardial fibrosis. (A) Immunofluorescence staining of intracellular NLRP3–ASC interaction examined by confocal microscopy. The NRCFs were labeled with anti-NLRP3 (red), anti-ASC (green), & DAPI (blue) (n = 3 per group). (B–G) After siRNA targeting NLRP3 transfection, the protein expressions of NLRP3, cleaved caspase-1 & collagen I were measured by western blot (n = 3 per group). (H) Representative images of immunofluorescence staining of intracellular alpha -SMA in the NG, HG, GF, & GF + siRNA groups (n = 3 per group). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35592403), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Epirubicin suppresses the NLRP3 inflammasome in presence of autophagy inhibitors. (A) LPS/ATP-induced IL-1 beta secretion from PM in the absence or presence of bafilomycin & 3-MA is shown. Means ± SEM from 10 (IL-1 beta ) independent experiments are shown. (B) Representative immunoblot of LC3b after epirubicin & LPS/ATP treatments. (C,D) Representative immunoblots of inflammasome & autophagy components after inhibition with (C) bafilomycin or (D) 3-MA in PM whole cell lysates. Cells were treated with indicated concentrations of epirubicin treatment over 24 h, followed by LPS (10 ng/mL) in the absence or presence of bafilomycin (300 nM) or 3-MA (10 mM) for 3 h & NLRP3 activation by ATP (1 mM, 1 h). Whole cell lysates were subjected to SDS-PAGE (10% gel) & processed for immunoblot analysis with specific antibodies. Here, beta -actin served as loading control. Statistical tests: one-sample t-test (A) ** p < 0.01, *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31905600), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

$Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Genetic or pharmacologic disruption of Caspase 1 blocks LukAB-induced cytokine secretion but not cell death.(A) THP1 cells were transfected with siRNA against Caspase 1, ASC or a non-targeting sequence. Cell lysates were collected & analyzed by immunoblot to confirm knockdown of pro-Caspase 1 & ASC. (B) THP1 siRNA cells were incubated with propidium iodide & intoxicated with 50 ng/mL LukAB for 1 hour then analyzed by flow cytometry. (C & D) THP1 siRNA cells were either primed with LTA (500ng/mL) (C) or untreated (D) then intoxicated with LukAB (50ng/mL) for 1 hr before culture supernatants were analyzed for release of IL-1 beta (C) or IL-18 (D). (E & F) Primary CD14+ human monocytes were incubated with the indicated concentration of z-YVAD-FMK or VX-765 for 30 minutes. Primary monocytes were incubated in the presence of propidium iodide (E) then intoxicated with LukAB (50ng/mL) & analyzed by flow cytometry. (F) Primary monocytes were intoxicated with LukAB (50ng/mL) & culture supernatants were analyzed for release of IL-18. Propidium iodide staining & IL-18 secretion are reported as a fraction of measurement in primary cells not treated with inhibitor. Bars represent the mean ± standard error of the mean for at least two independent experiments, each performed in triplicate. Asterisks indicate significance at a p-value of ≤ 0.05 by Tukey’s multiple comparisons post-test for 1-way or 2-way ANOVA, as appropriate. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1004970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Western Blot: Caspase-1 Antibody (14F468) - BSA Free [NB100-56565] - Probenecid reduces protein expression of NLRP1 inflammasome & ameliorates spatial learning deficits in aged rats. (A) Representative immunoblots of cleaved caspase-1, pannexin1 & P2X7R in hippocampal lysates of vehicle (Veh)-treated & probenecid (Pr)-treated 18-month-old rats. beta -tubulin was used as an internal control. (B) Densitometric analysis of immunoblots from brain lysates of cleaved caspase-1 (Casp1), P2X7 receptor (P2X7R), & pannexin1 (PanX1). (C-D) Aged animals underwent behavioral testing following either probenecid or vehicle treatment. (C) In a hippocampal-dependent spatial learning task via Morris water maze, latency to platform was measured on days 1-3 & 8-10. Probenecid-treatment improved latency to platform measured on the final day of testing (D) Mean path length was determined on day 10 of testing & probenecid-treated rats demonstrated significantly shorter mean path lengths than vehicle-treated controls. Drug treatment was administered twice daily for 3 days (days 7-9). Data are presented as mean +/- SEM *p < 0.05, **p < 0.005 compared to vehicle. N = 6-8/per group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22133203), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-5028

Alt Catalog# IMG-5028

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-56565AF350
Alexa Fluor 405	NB100-56565AF405
Alexa Fluor 488	NB100-56565AF488
Alexa Fluor 532	NB100-56565AF532
Alexa Fluor 594	NB100-56565AF594
Alexa Fluor 647	NB100-56565AF647
Alexa Fluor 700	NB100-56565AF700
Alexa Fluor 750	NB100-56565AF750
Alexa Fluor Plus 405	NB100-56565AFP405
Alexa Fluor Plus 488	NB100-56565AFP488
Alexa Fluor Plus 555	NB100-56565AFP555
Alexa Fluor Plus 594	NB100-56565AFP594
Alexa Fluor Plus 647	NB100-56565AFP647
Alexa Fluor Plus 680	NB100-56565AFP680
Allophycocyanin	NB100-56565APC
Biotin	NB100-56565B
CoraFluor 1	NB100-56565CL1
DyLight 350	NB100-56565UV
DyLight 405	NB100-56565V
DyLight 488	NB100-56565G
DyLight 550	NB100-56565R
DyLight 594	NB100-56565DL594
DyLight 650	NB100-56565C
DyLight 680	NB100-56565FR
DyLight 755	NB100-56565IR
FITC	NB100-56565F
HRP	NB100-56565H
Janelia Fluor 525	NB100-56565JF525
Janelia Fluor 549	NB100-56565JF549
Janelia Fluor 646	NB100-56565JF646
Janelia Fluor 669	NB100-56565JF669
PE	NB100-56565PE
PerCP	NB100-56565PCP
mFluor Violet 450 SE	NB100-56565MFV450
mFluor Violet 500 SE	NB100-56565MFV500
mFluor Violet 610 SE	NB100-56565MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56565SS
Azide and BSA Free	NBP2-33230

compare

(select up to 3 products from this page to compare)

MAB835

Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175)

(4 Reviews)

(112 Publications)

50 μg

MAB835

HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules." class="big_lightbox">

<b>Biological Strategies Validation. </b>Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # <a class=

F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Rac1 deficiency increases sensitivity towards UV-light-induced keratinocyte apoptosis in vitro. (a and b) Western blot analysis of cleaved caspase-3 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h (a) and at 12 h (b) with (UV) or without (no UV) UV-irradiation. (c) Densitometry analysis of cleaved caspase-3 at 6 h after UV-irradiation from Rac1 fl/fl and Rac1-EKO keratinocytes. (d) Quantification of CPDs by CPD ELISA carried out from genomic DNA isolated from Rac1 fl/fl and Rac1-EKO mouse primary keratinocytes immediately after UV-irradiation. Error bars show S.D. Asterisks show P-value<0.01 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Increase in UV-light-induced apoptosis in Rac1-deficient keratinocytes requires activation of caspase-8. (a) Western blot analysis of cleaved caspase-8 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (b) Densitometry analysis of fold change of cleaved caspase-8 in (a) normalized to GAPDH in Rac1 fl/fl and Rac1-EKO samples after UV-irradiation. (c) Western blot analysis of cleaved caspase-8 from CPDPL/Rac1-EKO epidermal lysates from mice kept in the dark or under the photoreactivtion lamp (PR). (d) Densitometric analysis of cleaved caspase-8 western blots in c. Error bars show S.D. (e) Western blot analysis of cleaved caspase-3 from wild-type (WT) and TNF receptor-1-deficient (TNFR-1 KO) cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (f) Western blot analysis of cleaved caspase-3 from TNFR-1 KO cultured keratinocytes incubated with DMSO or Rac1 inhibitor (EHT 1864) at 6 h with (UV) or without (no UV) UV-irradiation. GAPDH is used as a loading control. Numbers on the left denote molecular weights in kDa. * and *** represent P-value<0.05 and <0.001, respectively Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western blot analysis of high-mobility group box-1 (HMGB1), brain-derived neurotrophic factor (BDNF), synaptophysin, and cleaved caspase-3 in rat retinas. There is a significant increase in the expression of HMGB1 and cleaved caspase-3 and a significant decrease in the expression of BDNF and synaptophysin in the retinas of diabetic rats (D) compared with the nondiabetic control rats (N). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>BFSE decreases macrophage viability. THP-1 macrophage, or MDM cells, were exposure to air control or 1%, 2.5%, 5%, 10% BFSE or 10% CSE for 24 h. LDH from THP-1 macrophage or MDM cells was measured in supernatants after 24 h (n = 4) (A). THP-1 macrophage expression of Bcl-2 and PARP was assessed by western blot (B), band densitometry analysis of Bcl-2 (C) and PARP (D) was performed, data is a representation of three independent experiments, and was baselined to the air treated control sample, and normalized to beta -actin expression. Error bars represent 95% confidence intervals. Representative confocal images of active caspase-3 (E) and PAR (F), in THP-1 macrophage with quantitative MFI measurement of active caspase-3 (G) and PAR (H). (n = 4) *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30194323), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western blot analysis of rat retinas. Intravitreal administration of high-mobility group box-1 (HMGB1) induced a significant upregulation of the expression of HMGB1 and cleaved caspase-3 and a significant downregulation of the expression of brain-derived neurotrophic factor (BDNF) and synaptophysin compared with intravitreal administration of phosphate buffer saline (PBS). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.

Gamma herpesvirus 68 ( gamma HV68) replicates in alveolar epithelial cells (AECs). (A) Wild-type mice were infected with 5 × 104 PFU gamma HV68 on day 0. On day 7 after infection, frozen sections were prepared, and stained with a rabbit polyclonal antisera against gamma HV68, or with non-immune rabbit sera as control. The goat anti-rabbit secondary was linked to alkaline phosphatase. Vivid replication of gamma HV68 is visible in alveolar lining cells (original magnification × 100). Sections shown are representative of four mice examined. (B) AECs were isolated from lungs of Balb/c or TLR-9-/- mice treated with bleomycin plus gamma HV68 on day 21, and were cultured on fibronectin-coated slides (TiterTek). Sections were stained with antibodies (M30 Cytodeath), and the number of positive cells per high power field (HPF; ×400) were calculated (n = 30 HPF per genotype). (C) AECs were isolated from Balb/c or TLR-9-/- mice and cells were infected in vitro with 0.01 or 0.001 PFU gamma HV68 for 48 hours. Cell lysates were then analyzed for cleaved caspase 3 by western blotting. Data are from one experiment, representative of two. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21810214), licensed under a CC-BY license. Not internally tested by R&D Systems.

Assessment of the skin structure and apoptosis upon treatment with wound gels. Representative images and zoom-ins of the boxed areas of haematoxylin and eosin (H&E)-stained tape-stripped (TS) sections from biopsies (age 38 years) either left untreated or treated with indicated wound gels using a light microscope. The presence of caspase-3 expressing cells was evaluated using a caspase-3 (CASP3) antibody and visualisation with a secondary Alexa Fluor™ 546 AB (red). Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36261541), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC835U-100UG
Alexa Fluor 405	IC835V-100UG
Alexa Fluor 488	IC835G-025
Alexa Fluor 488	IC835G-100
Alexa Fluor 594	IC835T-100UG
Alexa Fluor 647	IC835R-100UG
Alexa Fluor 700	IC835N-100UG
Alexa Fluor 750	IC835S-100UG
Alexa Fluor Plus 405	FAB835AFP405-100UG
Alexa Fluor Plus 488	FAB835AFP488-100UG
Alexa Fluor Plus 555	FAB835AFP555-100UG
Alexa Fluor Plus 594	FAB835AFP594-100UG
Alexa Fluor Plus 647	FAB835AFP647-100UG
Alexa Fluor Plus 680	FAB835AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB835-SP
Carrier Free	IC835G-025
Carrier Free	IC835G-100
Carrier Free	IC835T-100UG
Carrier Free	IC835S-100UG
Carrier Free	IC835U-100UG
Carrier Free	IC835V-100UG
Carrier Free	IC835N-100UG
Carrier Free	IC835R-100UG

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NB100-56116

Caspase-8 Antibody - BSA Free

(2 Reviews)

(82 Publications)

0.05 ml

NB100-56116

Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tumor tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Anaplastic glioma (Grade III, left) and Gemistocytoma (Grade II, right) cores showing negative and positive staining for Caspase-8, respectively. B. Higher magnification of the Gemistocytoma tumor (from A).

Immunohistochemistry-Paraffin: Caspase-8 Antibody - (active/cleaved) [NB100-56116] - Sections from a brain tissue array stained for Caspase-8 expression using NB100-56116 at 1:2000. A. Normal brain stem (1) and cortex (2). B. Higher magnification of cortex (from A). C. Higher magnification of brain stem (from A). D. Normal cerebellum showing caspase-8 staining in the Purkinge cells.

Immunohistochemistry: Caspase-8 Antibody [NB100-56116] - Necroptosis marker p-MLKL can be detected in OA cartilage & after TNF/CHX stimulation.Exemplary images of impacted & differently stimulated cartilage explants after immunohistochemical staining of a cleaved CASP8, b RIPK3, & c p-MLKL. In addition, percentage of cleaved CASP8− (d) & p-MLKL− (e) positive cells was quantified (n = 3). Images were acquired using a 20× objective; the bars represent 200 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527653), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Caspase-8 Antibody [NB100-56116] - Expression of necroptosis- & apoptosis-related markers is elevated in OA cartilage.Necroptosis- & apoptosis-related markers in highly degenerated cartilage tissue of OA patients (ICRS grade ≥ 3) were determined by a gene expression analysis of apoptosis & necroptosis markers as well as immunohistochemical analysis (IHC) of b cleaved CASP8, c RIPK3, & d p-MLKL. Macroscopically intact cartilage (ICRS grade ≤ 1) served as control. Bars in the IHC images represent 200 µm. Statistical analysis was performed by an unpaired multiple t test; error bars indicate median & range from min to max; n = 13. Significant differences between groups were depicted as: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31527653), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NB100-56116] - Analysis of the cell type susceptible to apoptotic cell death in the hypothalamus of IRS2−/− mice. Immunoblots probed with antibodies against GFAP (A) & Tuj-1 (B) in the hypothalamus of wild-type (WT), non-diabetic IRS2-deficient (ND IRS2−/−) & diabetic IRS2-deficient (D IRS2−/−) mice. The average of three independent assays performed in duplicate is shown. Statistical significance by ANOVA: *P<0.05 vs WT mice. n=6/group. (C) Colocalization of TUNEL & GFAP in the hypothalamus of WT, ND IRS2−/− & D IRS2−/− mice. Arrows indicate astrocytes with apoptotic nuclei. (D) Colocalization of cleaved caspase-8 & GFAP, & of cleaved caspase-8 & NeuN, in the hypothalamus of WT, ND IRS2−/− & D IRS2−/− mice. Arrows indicate colocalization of GFAP & cleaved caspase-8. Scale bar: 50 µm; inset, 100 µm. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/doi/10.1242/dmm.023515/257174/Increased-oxidative-stress-and-apoptosis-in-the), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ma

Species Glossary

Reactivity: Hu, Mu, Rt, Ma

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5703

Alt Catalog# IMG-5703

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56116SS

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DCA100

Human Caspase-1/ICE Quantikine ELISA Kit

(47 Publications)

1 Kit

DCA100

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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NB100-56113

Caspase-3 Antibody - (active/cleaved) - BSA Free

(81 Publications)

0.05 ml

NB100-56113

<b>Biological Strategies Validation. </b>Caspase-3-Antibody-active-cleaved-Western-Blot-NB100-56113-img0007.jpg

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved)( NB100-56113) at 1:2000. Staining is seen in the nuclei of dying neurons (black arrow) but not in the morphologically normal nuclei (open arrows). Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using Caspase-3 Antibody - (active/cleaved) (NB100-56113) at 1:2000. Staining is seen in the nuclei of a subset of the cell population. Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

<b>Biological Strategies Validation. </b>Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 and 96 h and densitometry analysis of NF-kappa B (b), p16 (c), p21 (d), p27 (e), p53 (f), TRF1 (g), TRF2 (h), calnexin (i), NuMa (j), cleaved caspase 3 (k), Bcl-2 (l) was evaluated. Representative Western Blots are presented (a). Bars indicate SD, n = 3, ***/^^^p < 0.001, **/^^p < 0.01, */^p < 0.05, no indication—no statistical significance (one-way ANOVA and Dunnett’s a posteriori test)

Immunohistochemistry: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Pathology in DOX treated acute & recovery phase mice. Regions of myofiber loss & frank replacement fibrosis were noted, most commonly in atria (A, acute phase), & rarely in ventricles (D, recovery phase). These areas were accompanied by macrophage infiltration (B, E) & myofibroblast proliferation (C) consistent with fibroplasia. Rare myofibers were matrix metalloproteinase 2 (F, recovery phase animal) or caspase-3 positive (G, acute phase animal). Reticulin staining (A, D); Immunohistochemistry: Iba 1(B, E; macrophages), alpha SMA (C), MMP-2 (F) & cleaved caspase -3 (G) Bar = 100µm (A-C); 50µm (D-F); 20µm (G). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31263061), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Antidepressants-mediated effect on cellular protein content. HT-22 cells were treated with antidepressants for 48 & 96 h & densitometry analysis of NF-kappa B (b), p16 (c), p21 (d), p27 (e), p53 (f), TRF1 (g), TRF2 (h), calnexin (i), NuMa (j), cleaved caspase 3 (k), Bcl-2 (l) was evaluated. Representative Western Blots are presented (a). Bars indicate SD, n = 3, ***/^^^p < 0.001, **/^^p < 0.01, */^p < 0.05, no indication—no statistical significance (one-way ANOVA & Dunnett’s a posteriori test) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31278507), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Caspase-3 Antibody - (active/cleaved) [NB100-56113] - Morphological changes & expression of key apoptosis-related molecules in 4T1 cells after DE-EDCP treatment(A) Morphological changes of 4T1 cells exposed to various concentrations of DE-EDCP for 24h. (B) Immunofluorescence staining for Bcl-2 (green), Bax (green) & cleaved caspase-3 (green) together with DNA staining with DAPI (blue) in 4T1 cells incubated with DE-EDCP or cisplatin (31.25 μM) for 24h, as well as in untreated cells (magnification at x200). (C) mRNA expression of Bcl-2, Bax & caspase-3 quantified by RT-PCR in 4T1 cells after DE-EDCP 24h treatment. DE-EDCP treatment markedly increased the expression of Bax & caspase-3 mRNA & decreased the expression of Bcl-2 mRNA in 4T1 cells. beta -actin mRNA was used as an internal control. Data points are represented by the expression ratio & mean±SD fold of control in 4T1 cells. (* Bcl-2-: DE-EDCP vs. untreated p=0.03; DE-EDCP vs. cisplatin p=0.006; cislatin vs. untreated p=0.001; Bax-: DE-EDCP vs. untreated p=0.011; cislatin vs. untreated p=0.009; caspase-3-: DE-EDCP vs. untreated p=0.015; DE-EDCP vs. cisplatin p=0.021) Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25610), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ma

Species Glossary

Reactivity: Hu, Mu, Rt, Ma

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5700

Alt Catalog# IMG-5700

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56113SS

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MAB6215

Caspase-1 Antibody (661228) [Unconjugated]

(20 Publications)

100 μg

MAB6215

HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB6215U-100UG
Alexa Fluor 405	FAB6215V-100UG
Alexa Fluor 488	FAB6215G-100UG
Alexa Fluor 532	FAB6215X-100UG
Alexa Fluor 594	FAB6215T-100UG
Alexa Fluor 647	FAB6215R-100UG
Alexa Fluor 700	FAB6215N-100UG
Alexa Fluor 750	FAB6215S-100UG
Alexa Fluor Plus 405	FAB6215AFP405-100UG
Alexa Fluor Plus 488	FAB6215AFP488-100UG
Alexa Fluor Plus 555	FAB6215AFP555-100UG
Alexa Fluor Plus 594	FAB6215AFP594-100UG
Alexa Fluor Plus 647	FAB6215AFP647-100UG
Alexa Fluor Plus 680	FAB6215AFP680-100UG
Allophycocyanin	FAB6215A
Biotin	FAB6215B
CoraFluor 1	FAB6215CL1
DyLight 350	FAB6215D
DyLight 405	FAB6215E
DyLight 488	FAB6215K
DyLight 550	FAB6215L
DyLight 594	FAB6215M
DyLight 650	FAB6215W
DyLight 680	FAB6215Y
DyLight 755	FAB6215Z
FITC	FAB6215F
HRP	FAB6215H
Janelia Fluor 525	FAB6215JF525
Janelia Fluor 549	FAB6215I
Janelia Fluor 646	FAB6215J
Janelia Fluor 669	FAB6215JF669
PE	FAB6215P
PerCP	FAB6215C
mFluor Violet 450 SE	FAB6215MFV450
mFluor Violet 500 SE	FAB6215MFV500
mFluor Violet 610 SE	FAB6215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB6215-SP

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NB100-56564

Caspase-1 Antibody - BSA Free

(3 Publications)

0.1 mg

NB100-56564

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NB100-56564] - Human reactive lymph node stained for Caspase-1 expression (NB100-56564) using a DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.

Sevoflurane can increase the protein expression of NLRP3 inflammasome and its downstream inflammatory factors in prefrontal cortex. A–E Expression levels of NLRP3 inflammatory factors, related components, and downstream cytokine proteins. Western blot images were cropped to remove irrelevant sections of the image and display only the proteins of interest. Loading control: protein extracted from prefrontal cortex tissue from sham subjects treated with saline (lane1). Tissue used for Western blot analysis was collected 24-h after the last drug-administration. F–I NLRP3 inflammatory factors, related components, and downstream cytokine PCR results. Data are expressed as mean +/- SD; n = 4 for each experimental group. ***Compared with the Ctrl group, ###compared with the Sev group. P < 0.05 indicated a statistically significant difference Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36709248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5025

Alt Catalog# IMG-5025

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56564SS

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AF6215

Caspase-1 Antibody [Unconjugated]

(5 Publications)

100 μg

AF6215

HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

Western blot shows lysates of A431 human epithelial carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 0.25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Simple Western lane view shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Caspase-1 at approximately 52 kDa (as indicated) using 25 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Caspase-1 knockout A431 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Caspase-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6215) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Caspase-1 at approximately 50 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6215U-100UG
Alexa Fluor 405	AF6215V-100UG
Alexa Fluor 488	AF6215G-100UG
Alexa Fluor 532	AF6215X-100UG
Alexa Fluor 594	AF6215T-100UG
Alexa Fluor 647	AF6215R-100UG
Alexa Fluor 700	AF6215N-100UG
Alexa Fluor 750	AF6215S-100UG
Alexa Fluor Plus 405	AF6215AFP405-100UG
Alexa Fluor Plus 488	AF6215AFP488-100UG
Alexa Fluor Plus 555	AF6215AFP555-100UG
Alexa Fluor Plus 594	AF6215AFP594-100UG
Alexa Fluor Plus 647	AF6215AFP647-100UG
Alexa Fluor Plus 680	AF6215AFP680-100UG
Allophycocyanin	AF6215A
Biotin	AF6215B
CoraFluor 1	AF6215CL1
DyLight 350	AF6215D
DyLight 405	AF6215E
DyLight 488	AF6215K
DyLight 550	AF6215L
DyLight 594	AF6215M
DyLight 650	AF6215W
DyLight 680	AF6215Y
DyLight 755	AF6215Z
FITC	AF6215F
HRP	AF6215H
Janelia Fluor 525	AF6215JF525
Janelia Fluor 549	AF6215I
Janelia Fluor 646	AF6215J
Janelia Fluor 669	AF6215JF669
PE	AF6215P
PerCP	AF6215C
mFluor Violet 450 SE	AF6215MFV450
mFluor Violet 500 SE	AF6215MFV500
mFluor Violet 610 SE	AF6215MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6215-SP

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NBP2-75014

Mouse Caspase-1 ELISA Kit (Colorimetric)

(6 Publications)

1 Kit

NBP2-75014

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

31.25 - 2000 pg/mL

Sensitivity:

18.75 pg/mL

Sample Type:

Serum, plasma and other biological fluids

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NBP2-15713

Caspase-1 Antibody - BSA Free

(3 Publications)

0.1 ml

NBP2-15713

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NBP2-15713] - Mouse kidney. Caspase 1 stained by Caspase 1 antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Caspase-1 Antibody [NBP2-15713] - SW480 xenograft, using Caspase 1 alpha antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Inhibitor Target

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Caspase-3 Antibody [Unconjugated] - Active

Caspase-3 Antibody (31A1067) - (Pro and Active) - BSA Free

Caspase-1 Antibody (14F468) - BSA Free

Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175)

Caspase-8 Antibody - BSA Free

Human Caspase-1/ICE Quantikine ELISA Kit

Caspase-3 Antibody - (active/cleaved) - BSA Free

Caspase-1 Antibody (661228) [Unconjugated]

Caspase-1 Antibody - BSA Free

Caspase-1 Antibody [Unconjugated]

Mouse Caspase-1 ELISA Kit (Colorimetric)

Caspase-1 Antibody - BSA Free