| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83256-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80826-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87204-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92506-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89776-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73921 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90132-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87125-25ul | |||
![Western Blot: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Western blot analysis of TNF RII/TNFRSF1B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Western-Blot-NBP2-67218-img0009.jpg "Western Blot: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Western blot analysis of TNF RII/TNFRSF1B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunocytochemistry-Immunofluorescence-NBP2-67218-img0008.jpg)
![Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunohistochemistry-Paraffin-NBP2-67218-img0006.jpg)
![Flow Cytometry: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Flow-Cytometry-NBP2-67218-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunocytochemistry-Immunofluorescence-NBP2-67218-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunocytochemistry-Immunofluorescence-NBP2-67218-img0003.jpg)
![Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunohistochemistry-Paraffin-NBP2-67218-img0004.jpg)
![Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TNF-RII-TNFRSF1B-Antibody-JM113-01-Immunohistochemistry-Paraffin-NBP2-67218-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83256-img0007.jpg "Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.")
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0011.jpg)
![Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/HLTF-Antibody-Western-Blot-NBP1-83256-img0006.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0008.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0009.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0015.jpg "Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: TRAF-6 Antibody [NBP1-80826] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80826-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0013.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0011.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0012.jpg)
![Western Blot: ZNF313 Antibody [NBP1-87204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ZNF313-Antibody-Western-Blot-NBP1-87204-img0006.jpg "Western Blot: ZNF313 Antibody [NBP1-87204] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: ZNF313 Antibody [NBP1-87204] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87204-img0007.jpg)
![Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunohistochemistry-Paraffin-NBP1-87204-img0010.jpg)
![Western Blot: ZNF313 Antibody [NBP1-87204] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/ZNF313-Antibody-Western-Blot-NBP1-87204-img0005.jpg)
![Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunohistochemistry-Paraffin-NBP1-87204-img0002.jpg)
![Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunohistochemistry-Paraffin-NBP1-87204-img0008.jpg)
![Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunohistochemistry-Paraffin-NBP1-87204-img0009.jpg)
![Immunohistochemistry-Paraffin: ZNF313 Antibody [NBP1-87204] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ZNF313-Antibody-Immunohistochemistry-Paraffin-NBP1-87204-img0011.jpg)
![Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TIF1 alpha Antibody [NBP1-92506] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody [NBP1-92506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92506-img0006.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0012.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts while additional moderate cytoplasmic in Leydig cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0007.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NBP1-92506-img0010.jpg)
![Immunohistochemistry-Paraffin: TIF1 alpha Antibody [NBP1-92506] - Staining of human placenta shows moderate nuclear and weak cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-92506-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: TRIM8 Antibody [NBP1-89776] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/GERP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89776-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRIM8 Antibody [NBP1-89776] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.")
![Flow Cytometry: TRIM8 Antibody [NBP1-89776] - Knockdown of TRIM8 promotes differentiation & impairs GBM stemness & self‐renewal capacity. (A) Western blot analysis shows reduced CD133, NESTIN, SOX2, & c‐MYC, & upregulated GFAP in GBM neurosphere cells following TRIM8 knockdown by TRIM8 shRNA1 or TRIM8 shRNA2. NT shRNA = nontargeting control shRNA. Western blot also shows upregulated PIAS3 & reduced phosphorylated STAT3 (Tyr705) following shRNA knockdown of TRIM8. (B) ICC shows reduced expression of TRIM8 (red), NESTIN (white), & SOX2 (red) in TRIM8 shRNA1‐knockdown cells compared to NT shRNA cells. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (C) Flow cytometric analysis & statistical analysis of SOX2 expression in TRIM8‐overexpressing neurosphere cells. SOX2 was tagged by APC (blue: 676 nm) with TRIM8 tagged by PE (red: 555 nm). ***P < 0.001, ****P < 0.0001. Statistics: Data are means ± SD (n = 3), by nonparametric t‐test. (D,E) TRIM8 knockdown impairs neurosphere formation. Representative images of neurosphere expressing NT shRNA & TRIM8 shRNA1 at specific time points are shown. Scale bar = 30 μm. Statistical analysis of average sphere size: by one‐way analysis of variance (ANOVA). Data are means ± standard deviation (SD) (n = 6). **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Western blot analysis shows reduced TRIM8 & activated STAT3 (Tyr705), with increased GFAP expression following differentiation induced by serum. (G) ICC reveals reduced TRIM8 (red) & increased GFAP (white) after four days of differentiation induced by serum. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-31020241611359.jpg)
![Flow Cytometry: TRIM8 Antibody [NBP1-89776] - Ectopic expression of TRIM8 enhances stemness & self‐renewal of GBM‐derived neurospheres. (A) Western blot analysis shows upregulation of CD133, NESTIN, SOX2, & c‐MYC following ectopic expression of TRIM8‐GFP fusion protein. WB also shows reduced PIAS3 & increased phosphorylated STAT3 (Tyr705) & total STAT3 following TRIM8 overexpression. (B) ICC staining of TRIM8 (red) with stem cell markers NESTIN (white), & SOX2 (red) in neurosphere cells overexpressing TRIM8. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (C) Flow cytometric analysis & statistical analysis showing CD133 & SOX2 upregulation in neurosphere cells with TRIM8 overexpression. CD133 was tagged by PE (red: 555 nm) with TRIM8 tagged by APC (blue: 676 nm). SOX2 was tagged by APC (blue: 676 nm) with TRIM8 tagged by PE (red: 555 nm). ***P < 0.001, ****P < 0.0001. Statistics: Data are means ± SD (n = 3), by nonparametric t‐test. (D,E) Effects of TRIM8 overexpression on neurosphere formation. Representative images of neurospheres expressing GFP vector or TRIM8‐GFP vector at specific points. Scale bar = 30 μm. Statistical analysis of average sphere size: by one‐way analysis of variance (ANOVA). Data are means ± standard deviation (SD) (n = 6). **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Extreme limiting dilution assay (ELDA) shows that TRIM8 overexpression reduces the cell dose required for colony formation, supporting enhanced stem cell properties. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-31020241611351.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM8 Antibody [NBP1-89776] - TRIM8 is expressed in GBM samples & neurosphere cells. (A–C) Gene expression correlation of TRIM8 with STAT3,SOX2, & NESTIN using U133 mRNA data from TCGA. TRIM8 vs STAT3: Pearson's correlation coefficient: r = 0.36, P < 0.00001. TRIM8 vs SOX2: Pearson's correlation coefficient: r = 0.22, P < 0.00001. TRIM8 vs NESTIN: Pearson's correlation coefficient: r = 0.22, P < 0.00001. (D) Schematic representation of TRIM8 protein with RING, B‐box, & coiled‐coil domains. (E) TRIM8 copy number variation among 577 GBM samples in TCGA dataset. TRIM8 shows hemizygous deletion in 88.04% of cases. (F) Western blot analysis of TRIM8 protein among six normal human brain tissues (N1–N6) & five GBMs (G1–G5). (G) Immunohistochemical (IHC) staining of TRIM8 in normal brain & GBM tissues. TRIM8 (+) cells (indicated by arrow) are predominantly neurons in the normal brain & show cytoplasmic staining. TRIM8 expression in GBM is predominant in nuclei of tumor cells with modest cytoplasmic staining. Sections were counterstained with hematoxylin. Scale bar = 10 μm. (H) Reverse transcription PCR analysis & western blot analysis of TRIM8 among two normal cell lines & six patient‐derived GBM neurosphere cells. NHNP = normal human neural progenitor cell. (I) Immunocytochemical (ICC) staining of TRIM8 in neurosphere cells showing nuclear expression of TRIM8. TRIM8 was labeled in GFP, & nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-31020241612219.jpg)
![Immunocytochemistry/ Immunofluorescence: TRIM8 Antibody [NBP1-89776] - Ectopic expression of TRIM8 enhances stemness & self‐renewal of GBM‐derived neurospheres. (A) Western blot analysis shows upregulation of CD133, NESTIN, SOX2, & c‐MYC following ectopic expression of TRIM8‐GFP fusion protein. WB also shows reduced PIAS3 & increased phosphorylated STAT3 (Tyr705) & total STAT3 following TRIM8 overexpression. (B) ICC staining of TRIM8 (red) with stem cell markers NESTIN (white), & SOX2 (red) in neurosphere cells overexpressing TRIM8. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (C) Flow cytometric analysis & statistical analysis showing CD133 & SOX2 upregulation in neurosphere cells with TRIM8 overexpression. CD133 was tagged by PE (red: 555 nm) with TRIM8 tagged by APC (blue: 676 nm). SOX2 was tagged by APC (blue: 676 nm) with TRIM8 tagged by PE (red: 555 nm). ***P < 0.001, ****P < 0.0001. Statistics: Data are means ± SD (n = 3), by nonparametric t‐test. (D,E) Effects of TRIM8 overexpression on neurosphere formation. Representative images of neurospheres expressing GFP vector or TRIM8‐GFP vector at specific points. Scale bar = 30 μm. Statistical analysis of average sphere size: by one‐way analysis of variance (ANOVA). Data are means ± standard deviation (SD) (n = 6). **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Extreme limiting dilution assay (ELDA) shows that TRIM8 overexpression reduces the cell dose required for colony formation, supporting enhanced stem cell properties. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-310202416121925.jpg)
![Western Blot: TRIM8 Antibody [NBP1-89776] - TRIM8 is expressed in GBM samples & neurosphere cells. (A–C) Gene expression correlation of TRIM8 with STAT3,SOX2, & NESTIN using U133 mRNA data from TCGA. TRIM8 vs STAT3: Pearson's correlation coefficient: r = 0.36, P < 0.00001. TRIM8 vs SOX2: Pearson's correlation coefficient: r = 0.22, P < 0.00001. TRIM8 vs NESTIN: Pearson's correlation coefficient: r = 0.22, P < 0.00001. (D) Schematic representation of TRIM8 protein with RING, B‐box, & coiled‐coil domains. (E) TRIM8 copy number variation among 577 GBM samples in TCGA dataset. TRIM8 shows hemizygous deletion in 88.04% of cases. (F) Western blot analysis of TRIM8 protein among six normal human brain tissues (N1–N6) & five GBMs (G1–G5). (G) Immunohistochemical (IHC) staining of TRIM8 in normal brain & GBM tissues. TRIM8 (+) cells (indicated by arrow) are predominantly neurons in the normal brain & show cytoplasmic staining. TRIM8 expression in GBM is predominant in nuclei of tumor cells with modest cytoplasmic staining. Sections were counterstained with hematoxylin. Scale bar = 10 μm. (H) Reverse transcription PCR analysis & western blot analysis of TRIM8 among two normal cell lines & six patient‐derived GBM neurosphere cells. NHNP = normal human neural progenitor cell. (I) Immunocytochemical (ICC) staining of TRIM8 in neurosphere cells showing nuclear expression of TRIM8. TRIM8 was labeled in GFP, & nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-31020241612194.jpg)
![Western Blot: TRIM8 Antibody [NBP1-89776] - Knockdown of TRIM8 promotes differentiation & impairs GBM stemness & self‐renewal capacity. (A) Western blot analysis shows reduced CD133, NESTIN, SOX2, & c‐MYC, & upregulated GFAP in GBM neurosphere cells following TRIM8 knockdown by TRIM8 shRNA1 or TRIM8 shRNA2. NT shRNA = nontargeting control shRNA. Western blot also shows upregulated PIAS3 & reduced phosphorylated STAT3 (Tyr705) following shRNA knockdown of TRIM8. (B) ICC shows reduced expression of TRIM8 (red), NESTIN (white), & SOX2 (red) in TRIM8 shRNA1‐knockdown cells compared to NT shRNA cells. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (C) Flow cytometric analysis & statistical analysis of SOX2 expression in TRIM8‐overexpressing neurosphere cells. SOX2 was tagged by APC (blue: 676 nm) with TRIM8 tagged by PE (red: 555 nm). ***P < 0.001, ****P < 0.0001. Statistics: Data are means ± SD (n = 3), by nonparametric t‐test. (D,E) TRIM8 knockdown impairs neurosphere formation. Representative images of neurosphere expressing NT shRNA & TRIM8 shRNA1 at specific time points are shown. Scale bar = 30 μm. Statistical analysis of average sphere size: by one‐way analysis of variance (ANOVA). Data are means ± standard deviation (SD) (n = 6). **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Western blot analysis shows reduced TRIM8 & activated STAT3 (Tyr705), with increased GFAP expression following differentiation induced by serum. (G) ICC reveals reduced TRIM8 (red) & increased GFAP (white) after four days of differentiation induced by serum. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-310202416114748.jpg)
![Western Blot: TRIM8 Antibody [NBP1-89776] - TRIM8 modulates GBM stemness via a positive loop of PIAS3‐STAT3 pathway. (A) Western blot analysis of PIAS3 accumulation following TRIM8 overexpression in the presence of MG132 (20 μm) for four hours. (B–E) Western blot analyses showing that the half‐life of PIAS3 was reduced by TRIM8 overexpression & increased by TRIM8 knockdown after treating cells with 100 μg·mL−1 cycloheximide (CHX) for 0, 2, 4, 6, & 8 h in two different neurosphere cell lines. (F) Western blot showing that interleukin 6 (IL‐6; 10 & 50 ng·mL−1) induced phosphorylated STAT3 (Tyr705) & also enhanced expression of STAT3, c‐MYC, SOX2, & TRIM8. (G) Western blot showing that the STAT3 Inhibitor XI (STX‐0119; 10 & 100 μm) selectively suppresses phosphorylated STAT3 (Tyr705) & reduces expression of STAT3, c‐MYC, SOX2, & TRIM8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-310202416121993.jpg)
![Western Blot: TRIM8 Antibody [NBP1-89776] - Ectopic expression of TRIM8 enhances stemness & self‐renewal of GBM‐derived neurospheres. (A) Western blot analysis shows upregulation of CD133, NESTIN, SOX2, & c‐MYC following ectopic expression of TRIM8‐GFP fusion protein. WB also shows reduced PIAS3 & increased phosphorylated STAT3 (Tyr705) & total STAT3 following TRIM8 overexpression. (B) ICC staining of TRIM8 (red) with stem cell markers NESTIN (white), & SOX2 (red) in neurosphere cells overexpressing TRIM8. Nuclei were counterstained with DAPI (blue). Scale bar = 10 μm. (C) Flow cytometric analysis & statistical analysis showing CD133 & SOX2 upregulation in neurosphere cells with TRIM8 overexpression. CD133 was tagged by PE (red: 555 nm) with TRIM8 tagged by APC (blue: 676 nm). SOX2 was tagged by APC (blue: 676 nm) with TRIM8 tagged by PE (red: 555 nm). ***P < 0.001, ****P < 0.0001. Statistics: Data are means ± SD (n = 3), by nonparametric t‐test. (D,E) Effects of TRIM8 overexpression on neurosphere formation. Representative images of neurospheres expressing GFP vector or TRIM8‐GFP vector at specific points. Scale bar = 30 μm. Statistical analysis of average sphere size: by one‐way analysis of variance (ANOVA). Data are means ± standard deviation (SD) (n = 6). **P < 0.01, ***P < 0.001, ****P < 0.0001. (F) Extreme limiting dilution assay (ELDA) shows that TRIM8 overexpression reduces the cell dose required for colony formation, supporting enhanced stem cell properties. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-310202416121954.jpg)
![Western Blot: TRIM8 Antibody [NBP1-89776] - TRIM8 modulates GBM stemness via a positive loop of PIAS3‐STAT3 pathway. (A) Western blot analysis of PIAS3 accumulation following TRIM8 overexpression in the presence of MG132 (20 μm) for four hours. (B–E) Western blot analyses showing that the half‐life of PIAS3 was reduced by TRIM8 overexpression & increased by TRIM8 knockdown after treating cells with 100 μg·mL−1 cycloheximide (CHX) for 0, 2, 4, 6, & 8 h in two different neurosphere cell lines. (F) Western blot showing that interleukin 6 (IL‐6; 10 & 50 ng·mL−1) induced phosphorylated STAT3 (Tyr705) & also enhanced expression of STAT3, c‐MYC, SOX2, & TRIM8. (G) Western blot showing that the STAT3 Inhibitor XI (STX‐0119; 10 & 100 μm) selectively suppresses phosphorylated STAT3 (Tyr705) & reduces expression of STAT3, c‐MYC, SOX2, & TRIM8. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28100038), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89776_rabbit-polyclonal-trim8-antibody-310202416114771.jpg)
![Western Blot: RNF113B Antibody (7A1) [NBP2-01644] Analysis of extracts (35ug) from 9 different cell lines by using anti-RNF113B monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Western-Blot-NBP2-01644-img0008.jpg "Western Blot: RNF113B Antibody (7A1) [NBP2-01644] Analysis of extracts (35ug) from 9 different cell lines by using anti-RNF113B monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: RNF113B Antibody (7A1) [NBP2-01644] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF113B.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunocytochemistry-Immunofluorescence-NBP2-01644-img0007.jpg)
![Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Human breast tissue using anti-RNF113B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunohistochemistry-Paraffin-NBP2-01644-img0006.jpg)
![Western Blot: RNF113B Antibody (7A1) [NBP2-01644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF113B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF113B.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Western-Blot-NBP2-01644-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RNF113B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunohistochemistry-Paraffin-NBP2-01644-img0002.jpg)
![Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RNF113B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunohistochemistry-Paraffin-NBP2-01644-img0003.jpg)
![Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RNF113B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunohistochemistry-Paraffin-NBP2-01644-img0004.jpg)
![Immunohistochemistry-Paraffin: RNF113B Antibody (7A1) [NBP2-01644] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RNF113B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RNF113B-Antibody-7A1-Immunohistochemistry-Paraffin-NBP2-01644-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/cIAP-2-HIAP-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90132-img0015.jpg "Immunocytochemistry/Immunofluorescence: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![<b>Biological Strategies Validation. </b>Western Blot: cIAP-2/HIAP-1 Antibody [NBP1-90132] - Mouse neonatal cardiac myocytes in under normal and hypoxic conidtions. Wb image submitted by verified customer review.](http://images.novusbio.com/fullsize/cIAP-2-HIAP-1-Antibody-Western-Blot-NBP1-90132-img0017.jpg)
![Western Blot: TRAF-5 Antibody [NB100-56178] - TRAF5 in normal human tissues using this antibody. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 degradation fragments.](http://images.novusbio.com/fullsize/TRAF-5-Antibody-Western-Blot-NB100-56178-img0009.jpg "Western Blot: TRAF-5 Antibody [NB100-56178] - TRAF5 in normal human tissues using this antibody. TRAF5 is observed at ~64 kDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF5 degradation fragments.")
![Immunohistochemistry-Paraffin: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse tissue stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A, thymus. B, bone marrow.](http://images.novusbio.com/fullsize/TRAF-5-Antibody-Immunohistochemistry-Paraffin-NB100-56178-img0010.jpg)
![Immunohistochemistry: TRAF-5 Antibody [NB100-56178] - Formalin-fixed, paraffin-embedded mouse spleen stained for TRAF5 expression using NB100-56178 at 1:2000. Hematoxylin-eosin counterstain. A1 is a higher magnification of A.](http://images.novusbio.com/fullsize/TRAF-5-Antibody-Immunohistochemistry-NB100-56178-img0007.jpg)
![Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-2406] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MuRF1-TRIM63-Antibody-NB100-2406-img0001.jpg "Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody [NB100-2406] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human cerebral cortex, kidney, liver and testis using Anti-TRAIP antibody NBP1-87125 (A) shows similar protein distribution across tissues to independent antibody NBP2-48655 (B).](http://images.novusbio.com/fullsize/TRAIP-Antibody-Immunohistochemistry-Paraffin-NBP1-87125-img0007.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human cerebral cortex, kidney, liver and testis using Anti-TRAIP antibody NBP1-87125 (A) shows similar protein distribution across tissues to independent antibody NBP2-48655 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: TRAIP Antibody [NBP1-87125] - TRAIP activity is required for Mcm7 ubiquitylation in mitosis. The same experiment from Fig 3B but with addition of the RNF213 blot. An asterisk](http://images.novusbio.com/fullsize/TRAIP-Antibody-Western-Blot-NBP1-87125-img0008.jpg)
![Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human liver using Anti-TRAIP antibody.](http://images.novusbio.com/fullsize/TRAIP-Antibody-Immunohistochemistry-Paraffin-NBP1-87125-img0003.jpg)
![Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human testis using Anti-TRAIP antibody.](http://images.novusbio.com/fullsize/TRAIP-Antibody-Immunohistochemistry-Paraffin-NBP1-87125-img0004.jpg)
![Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human cerebral cortex using Anti-TRAIP antibody.](http://images.novusbio.com/fullsize/TRAIP-Antibody-Immunohistochemistry-Paraffin-NBP1-87125-img0005.jpg)
![Immunohistochemistry-Paraffin: TRAIP Antibody [NBP1-87125] - Staining of human kidney using Anti-TRAIP antibody.](http://images.novusbio.com/fullsize/TRAIP-Antibody-Immunohistochemistry-Paraffin-NBP1-87125-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)