| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44056 | |||
| BSA Free | NBP2-61628-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54544-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86990-25ul | |||
![Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Western-Blot-NBP2-61628-img0010.jpg "Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of A-431 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunocytochemistry-Immunofluorescence-NBP2-61628-img0011.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human skeletal muscle shows absence of immunoreactivity in muscle fibers (negative control).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0008.jpg)
![Western Blot: beta-Catenin Antibody (CL3689) [NBP2-61628] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Western-Blot-NBP2-61628-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of U-251 cells using the Anti-CTNNB1 monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunocytochemistry-Immunofluorescence-NBP2-61628-img0012.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human endometrium shows strong membranous immunoreactivity in glandular epithelium.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0001.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human kidney shows strong membranous positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0002.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human duodenum shows strong membranous immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human cervix shows strong membranous positivity in epithelial cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-NBP2-61628-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human breast cancer shows membranous immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human prostate cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CL3689) [NBP2-61628] - Staining of human renal cancer shows membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CL3689-Immunohistochemistry-Paraffin-NBP2-61628-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human cell line U-138MG.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Western-Blot-NBP2-88916-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of HeLa cells using the Anti-SMAD2 monoclonal antibody, showing specific staining in the cytosol and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunocytochemistry-Immunofluorescence-NBP2-88916-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human breast cancer shows moderate cytoplasmic positivity.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human Fallopian tube shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human pancreas shows low cytoplasmic positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0004.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human testis shows weak to moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/Smad2-Antibody-CL8472-Immunohistochemistry-Paraffin-NBP2-88916-img0006.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.")
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Wild-type (WT) and beta-Catenin knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-GT2169-Western-Blot-NBP3-13530-img0004.jpg)
![Western Blot: beta-Catenin Antibody (GT2169) [NBP3-13530] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta-Catenin antibody (NBP3-13530) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-91020242091497.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (GT2169) [NBP3-13530] - beta-Catenin antibody detects beta-Catenin protein at cell membrane by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta-Catenin stained by beta-Catenin antibody (NBP3-13530) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-910202420131215.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Catenin protein stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunocytochemistry-immunofluorescence-2611202518375021.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251843450.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human pancreas.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025196218.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-26112025184740.jpg)
![beta Catenin antibody [GT2169] detects beta Catenin protein by immunohistochemical analysis.Sample: Paraffin-embedded human appendix.beta Catenin stained by beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-immunohistochemistry-paraffin-261120251829266.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] (NBP3-13530) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13530_mouse-beta-catenin-mab-gt2169-western-blot-2611202518372424.jpg)
![Western Blot: Wnt-7b Antibody [NBP1-59564] - Titration: 0.2-1 ug/ml, Positive Control: Human kidney.](http://images.novusbio.com/fullsize/Wnt-7b-Antibody-Western-Blot-NBP1-59564-img0007.jpg "Western Blot: Wnt-7b Antibody [NBP1-59564] - Titration: 0.2-1 ug/ml, Positive Control: Human kidney.")
![Immunohistochemistry-Paraffin: Wnt-7b Antibody [NBP1-59564] - Human Brain, cortex tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/Wnt-7b-Antibody-Immunohistochemistry-Paraffin-NBP1-59564-img0006.jpg)
![Western Blot: Wnt-3a Antibody [NBP1-74183] - Sample Tissue: Mouse Heart, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0009.jpg "Western Blot: Wnt-3a Antibody [NBP1-74183] - Sample Tissue: Mouse Heart, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%")
![Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183] - Human placenta tissue at an antibody concentration of Dilution: 1:500.](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-Immunohistochemistry-Paraffin-NBP1-74183-img0012.jpg)
![Western Blot: Wnt-3a Antibody [NBP1-74183] - Titration: 1 ug/ml Positive Control: Mouse Heart lysate.](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0011.jpg)
![Western Blot: Wnt-3a Antibody [NBP1-74183] - Mouse Heart. Lane A: Primary Antibody. Lane B: Primary Antibody + Blocking Peptide.](http://images.novusbio.com/fullsize/Wnt-3a-Antibody-Western-Blot-NBP1-74183-img0010.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0009.jpg "Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5 Analysis of LEF1 expression in MES-SA/Dx5.")
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi ( Cat # H00051176-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody (M01), clone 3H5 (Cat # H00051176-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0006.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - LEF1 monoclonal antibody (M01), clone 3H5. Analysis of LEF1 expression in HL-60.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0008.jpg)
![Western Blot: LEF1 Antibody (3H5) [H00051176-M01] - Analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody (M01), clone 3H5.Lane 1: LEF1 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Western-Blot-H00051176-M01-img0010.jpg)
![Sandwich ELISA: LEF1 Antibody (3H5) [H00051176-M01] - Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LEF1-Antibody-3H5-Sandwich-ELISA-H00051176-M01-img0007.jpg)
![Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Western-Blot-NBP2-53358-img0004.jpg "Western Blot: beta-Catenin Antibody (15B8) [NBP2-53358] - Western Blot Analysis of HeLa cell lysate using beta-Catenin Antibody (15B8).")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (15B8) [NBP2-53358] - Confocal immunofluorescence image of HeLa cells using beta-Catenin Antibody (15B8) labeled is Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunocytochemistry-Immunofluorescence-NBP2-53358-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (15B8) [NBP2-53358] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin (p120) Monoclonal Antibody (15B8).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-15B8-Immunohistochemistry-Paraffin-NBP2-53358-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/SIAH1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-974-img0001.jpg "Immunocytochemistry/Immunofluorescence: SIAH1 Antibody [NB300-974] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing mitochondrial/cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0006.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Staining Phospho-Smad3(S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunocytochemistry-Immunofluorescence-NBP2-67513-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0009.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Phospho-Smad3(S423/S425) on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0003.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Lane 1: K562 cells, whole cell lysate, 10ug/lane, Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-Smad3(S423/S425) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 48 kDa Observed band size: 55 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0004.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on different lysates with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0008.jpg)
![Western Blot: Calcineurin B Antibody [NBP2-15664] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calcineurin-B-Antibody-Western-Blot-NBP2-15664-img0005.jpg "Western Blot: Calcineurin B Antibody [NBP2-15664] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Calcineurin B Antibody [NBP2-15664] - Calcineurin B antibody detects Calcineurin B protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcineurin B stained by Calcineurin B antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Calcineurin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15664-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcineurin B Antibody [NBP2-15664] - Analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Calcineurin-B-Antibody-Immunohistochemistry-Paraffin-NBP2-15664-img0003.jpg)
![Western Blot: Calcineurin B Antibody [NBP2-15664] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Calcineurin-B-Antibody-Western-Blot-NBP2-15664-img0001.jpg)
![Western Blot: Calcineurin B Antibody [NBP2-15664] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody diluted at 1:50000.](http://images.novusbio.com/fullsize/Calcineurin-B-Antibody-Western-Blot-NBP2-15664-img0004.jpg)
![Western Blot: Calcineurin B Antibody [NBP2-15664] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP2-15664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15664_rabbit-polyclonal-calcineurin-b-antibody-91020242021924.jpg)
![Western Blot: Calcineurin B Antibody [NBP2-15664] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calcineurin B antibody (NBP2-15664) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15664_rabbit-polyclonal-calcineurin-b-antibody-910202419593563.jpg)
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20199-img0005.jpg "Immunocytochemistry/Immunofluorescence: ROCK2 Antibody [NBP2-20199] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: ROCK2 Antibody [NBP2-20199] - Human esophagous cancer, using ROCK2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Immunohistochemistry-Paraffin-NBP2-20199-img0004.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0001.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 5% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0002.jpg)
![Western Blot: ROCK2 Antibody [NBP2-20199] - Sample (50 ug of whole cell lysate) A: Rat brain, 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NBP2-20199-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [N3C1], Internal (NBP2-20199) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20199_rabbit-polyclonal-rock2-antibody-western-blot-26112025196273.jpg)