| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92690SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56074SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77441SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70653 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76695-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76648SS | |||
![Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MTA2-Antibody-Western-Blot-NB100-56483-img0004.jpg "Western Blot: MTA2 Antibody [NB100-56483] - Lysate from Jurkat cells, in the 1) absence and 2) presence of immunizing peptide, probed with this antibody at 0.5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: MTA2 Antibody [NB100-56483] - This antibody was tested in HEK293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/MTA2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56483-img0001.jpg)
![Immunohistochemistry: MTA2 Antibody [NB100-56483] - Analysis of Human Testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/MTA2-Antibody-Immunohistochemistry-NB100-56483-img0003.jpg)
![Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Western-Blot-NBP1-92690-img0002.jpg "Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - MDA-MB-231 cells stained for Gal-3 (red, arrows), shown with DAPI counterstained nuclei (blue). Negative control received non-specific antibody instead of primary antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to galectin-3, NBP1-92690, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The NBP1-92690 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while the MAP2 antibody labels dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0001.jpg)
![Western Blot: APAF1 Antibody [NB100-56074] - Analysis of Apaf 1 using this antibody. Jurkat: human T-cell leukemia cell line lysate. RS11-846: human B-cell leukemia cell line lysate. Human thymus: tissue lysate.](http://images.novusbio.com/fullsize/APAF1-Antibody-Western-Blot-NB100-56074-img0004.jpg "Western Blot: APAF1 Antibody [NB100-56074] - Analysis of Apaf 1 using this antibody. Jurkat: human T-cell leukemia cell line lysate. RS11-846: human B-cell leukemia cell line lysate. Human thymus: tissue lysate.")
![Immunocytochemistry/Immunofluorescence: APAF1 Antibody [NB100-56074] - Formalin-fixed, paraffin-embedded sections from a human brain tumor tissue microarray stained for Apaf 1 expression using NB100-56074 at 1:2000. A and B. Tissue microarray overview. A1 and B1, higher magnification of cores from A and B, respectively. Differential expression of Apaf 1 is seen between patient samples. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/APAF1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56074-img0002.jpg)
![Immunohistochemistry: APAF-1 Antibody [NB100-56074] - APAF1 Antibody [NB100-56074] - Formalin-fixed, paraffin-embedded tissue sections of human brain tumors stained for Apaf 1 expression using NB100-56074 at 1:2000. A. Gemistocytoma (grade II). B. Anaplastic glioma. A high level of Apaf 1 was observed in the gemistocytoma whereas a low level was seen in the more malignant anaplastic glioma. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/APAF-1-Antibody-Immunohistochemistry-NB100-56074-img0006.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Western-Blot-NBP2-67399-img0007.jpg "Western Blot: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of CD34 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: TF-1 cell lysate Lane 2: Mouse lung tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - (G) CD34 + microvascular staining in EOC tissue. Correlation between HE4 serum levels and CD34 + area in patient EOC tissue (n = 14). **p < 0.005, ****p < 0.0005. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-020-65353-x), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0008.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD34 Antibody (SI16-01) [NBP2-67399] - Staining CD34 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunocytochemistry-Immunofluorescence-NBP2-67399-img0003.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0004.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0006.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0005.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human tonsil tissue labeling CD34 with Rabbit anti-CD34 antibody washed with PBS, and then probed with the primary antibody green) at 1/50 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0009.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0010.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SI16-01) [NBP2-67399] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-CD34 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD34-Antibody-SI16-01-Immunohistochemistry-Paraffin-NBP2-67399-img0011.jpg)
![Western Blot: SGLT2/SLC5A2 Antibody [NBP2-77441] - Total protein from human Hek293 and Jurkat cells was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SLC5A2 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/SGLT2-SLC5A2-Antibody-Western-Blot-NBP2-77441-img0002.jpg "Western Blot: SGLT2/SLC5A2 Antibody [NBP2-77441] - Total protein from human Hek293 and Jurkat cells was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-SLC5A2 in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: SGLT2/SLC5A2 Antibody [NBP2-77441] - IHC staining of human kidney using SLC5A2 antibody (NBP2-77441).](http://images.novusbio.com/fullsize/SGLT2-SLC5A2-Antibody-Immunohistochemistry-Paraffin-NBP2-77441-img0001.jpg)
![Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0008.jpg "Western Blot: DDX3 Antibody [NB200-194] - Detection of human and mouse DDX3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX3 antibody NB200-194 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of human DDX3 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0010.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DDX3 Antibody [NB200-194] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-194 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-195 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).](http://images.novusbio.com/fullsize/DDX3-Antibody-Western-Blot-NB200-194-img0005.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Sample: FFPE section of human lung cancer pleural effusion (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX3 used at a dilution of 1:200 (1ug/ml). Detection: Red fluorescence (A120-601D4) and DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0006.jpg)
![Immunohistochemistry: DDX3 Antibody [NB200-194] - Detection of mouse DDX3 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DDX3 (NB200-194 lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunohistochemistry-NB200-194-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DDX3 Antibody [NB200-194] - Detection of human DDX3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX3 antibody NB200-194 (lot NB200-194-2) used for IP at 6 ug per reaction. DDX3 was also immunoprecipitated by a previous lot of this antibody (lot NB200-194-1) and rabbit anti-DDX3 antibody NB200-196. For blotting immunoprecipitated DDX3, NB200-194 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 seconds.](http://images.novusbio.com/fullsize/DDX3-Antibody-Immunoprecipitation-NB200-194-img0007.jpg)
![Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-OTI4F10-Western-Blot-NBP2-01152-img0010.jpg "Western Blot: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Analysis of extracts (35ug) from 2 different Human cell lines, dilution (1:500).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (OTI4F10) [NBP2-01152] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4F10-Immunocytochemistry-Immunofluorescence-NBP2-01152-img0008.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Human Kidney tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0005.jpg)
![Flow Cytometry: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ErbB2/HER2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Flow-Cytometry-NBP2-01152-img0001.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0002.jpg)
![Western Blot: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - 1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8:Colon.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Western-Blot-NBP2-01152-img0007.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0003.jpg)
![Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (4F10) [NBP2-01152] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ErbB2/HER2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ErbB2-HER2-Antibody-4F10-Immunohistochemistry-Paraffin-NBP2-01152-img0004.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Western-Blot-NB100-692-img0003.jpg "Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - Hydrolysis of MNNG & TMZ to active cation and deficient MMR & MGMT protein expression in cancer cells results in increased colony survival after MNNG exposure. MMR & MGMT protein expression in six cell lines by SDS PAGE and immunoblot. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0074071) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin fixed paraffin embedded human tonsil stained with MGMT antibody.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0002.jpg)
![Immunohistochemistry-Paraffin: MGMT Antibody (MT 3.1) [NB100-692] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MGMT MT 3.1 (Cat. #NB 100-692) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.](http://images.novusbio.com/fullsize/MGMT-Antibody-MT-3-1-Immunohistochemistry-Paraffin-NB100-692-img0001.jpg)
![Western Blot: MGMT Antibody (MT 3.1) [NB100-692] - MMR protein expression & hMutS alpha activity decreases while hprt mutation rates & MNNG resistance increases in MGMT & MMR negative subclones after repeated exposure to MNNG.A. MMR protein expression, each fluorescent protein band was measured against a loading control (p62) in the same lane by Alpha lnnotech Fluorochem HD2, histograms produced by Prism GraphPad software, error bars indicate SD. U251 not represented in histogram because hMSH6 & hMSH2 expression is completely absent (Figure 1). Asterisks (*) denote statistically significant differences at P < 0.05 between each subclone protein expression & HeLa MR for the designated MMR protein. Statistical significance determined by student t-test using Prism GraphPad software. Each experiment was performed a minimum of 3 times. B. hMutS alpha binding activity of equal nuclear protein concentration from nuclear extracts of each cell line by EMSA using [32P]-69mer oligomers with either G:T or 06meG:T located in the center. C. hprt mutation rates in two sequentially isolated HeLa MNNGR clones as compared to HeLa MR. D. Classic colony survival of HeLa MNNG4 & U251R subclones indicates significant resistance to 0.2 µM MNNG as compared to HeLa MR & U251 parental cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24019948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-692_mouse-monoclonal-mgmt-antibody-mt-3-1-31020241612636.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0011.jpg "Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0004.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0009.jpg)
![Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/TWEAK-TNFSF12-Antibody-Western-Blot-NBP1-76695-img0010.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-TWEAK/TNFSF12 antibody at 10 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420115594.jpg)
![Immunohistochemistry: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TWEAK/TNFSF12 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202420272466.gif)
![Immunocytochemistry/ Immunofluorescence: TWEAK/TNFSF12 Antibody - BSA Free [NBP1-76695] - Validation of TWEAK/TNFSF12 in Mouse Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TWEAK/TNFSF12 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76695_rabbit-polyclonal-tweak-tnfsf12-antibody-2711202419514021.gif)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420175183.gif "Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Rat Liver Tissue. Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419261860.gif)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in human brain tissue with Beclin-1 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0004.jpg)
![Immunohistochemistry: Beclin 1 Antibody [NBP1-76648] - Beclin-1 in mouse brain tissue with Beclin-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Beclin-1-Antibody-Immunohistochemistry-NBP1-76648-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Brain Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain cells labeling Beclin 1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419451274.gif)
![Immunohistochemistry: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in Mouse Kidney Tissue.Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beclin 1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120241958491.gif)
![Immunocytochemistry/ Immunofluorescence: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation of Beclin 1 in HELA Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling Beclin 1 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419251598.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Human Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-271120242016947.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Mouse Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202420115589.gif)
![Western Blot: Beclin 1 Antibody - BSA Free [NBP1-76648] - Validation in Rat Cell Lines.Loading: 15 ug of lysate Antibodies: Beclin 1, 1 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76648_rabbit-polyclonal-beclin-1-antibody-2711202419592041.gif)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0004.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73605-img0008.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0009.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0007.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0001.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0002.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0003.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0010.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0005.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0006.jpg)