| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85664-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92995SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89929-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83256-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43954 | |||
| BSA Free | NBP2-34495-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89463-25ul | |||
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0025.jpg "Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.")
![Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Immunocytochemistry-Immunofluorescence-H00000573-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Immunohistochemistry-Paraffin-H00000573-M02-img0018.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate (38.878 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0013.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3 Analysis of BAG1 expression in HeLa.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0020.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in PC-12.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0021.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in LNCaP.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0022.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0023.jpg)
![Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Jurkat.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Western-Blot-H00000573-M02-img0024.jpg)
![Sandwich ELISA: Bag-1 Antibody (2D3) [H00000573-M02] - Detection limit for recombinant GST tagged BAG1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Bag-1-Antibody-2D3-Sandwich-ELISA-H00000573-M02-img0019.jpg)
![Immunohistochemistry-Paraffin: TTC11 Antibody [NBP1-85664] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TTC11-Antibody-Immunohistochemistry-Paraffin-NBP1-85664-img0016.jpg "Immunohistochemistry-Paraffin: TTC11 Antibody [NBP1-85664] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: TTC11 Antibody [NBP1-85664] - Staining of human Fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TTC11-Antibody-Immunohistochemistry-Paraffin-NBP1-85664-img0015.jpg)
![Immunohistochemistry-Paraffin: TTC11 Antibody [NBP1-85664] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/TTC11-Antibody-Immunohistochemistry-Paraffin-NBP1-85664-img0017.jpg)
![Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Knockout-Validated-NBP2-92995-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p62/SQSTM1 knockout HeLa cell line (KO). PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # 2275-PC-100) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Western blot shows lysates of A172 human glioblastoma cell line and RT-4 human bladder carcinoma cell line. PVDF membrane was probed with 1 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for p62/SQSTM1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Western-Blot-NBP2-92995-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - p62/SQSTM1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with chloroquine (left) or untreated (right) using Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Immunocytochemistry-Immunofluorescence-NBP2-92995-img0002.jpg)
![Immunohistochemistry-Paraffin: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - p62/SQSTM1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog #NBP2-92995) at 10 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Immunohistochemistry-Paraffin-NBP2-92995-img0003.jpg)
![Simple Western: p62/SQSTM1 Antibody (2533B) [NBP2-92995] - Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for p62/SQSTM1 at approximately 64 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human p62/SQSTM1 Monoclonal Antibody (Catalog # NBP2-92995). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p62-SQSTM1-Antibody-2533B-Simple-Western-NBP2-92995-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human cell line A-431 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89929-img0003.jpg "Immunocytochemistry/Immunofluorescence: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human cell line A-431 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Blooms Syndrome Protein Blm Antibody [NBP1-89929] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Blooms-Syndrome-Protein-Blm-Antibody-Immunohistochemistry-Paraffin-NBP1-89929-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83256-img0007.jpg "Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.")
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0011.jpg)
![Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/HLTF-Antibody-Western-Blot-NBP1-83256-img0006.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0008.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0009.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining in human skin and skeletal muscle tissues. Corresponding p53 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-P53 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U2OS cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0014.jpg)
![Western Blot: p53 Antibody (CL2199) [NBP2-34495] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Western-Blot-NBP2-34495-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining of human cell line A431 showing cell cycle dependent nuclear (without nucleoli)Staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in MCF7 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (CL2199) [NBP2-34495] - Staining in U251 cell line with Anti-p53 monoclonal antibody, showing cell cycle dependent nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunocytochemistry-Immunofluorescence-NBP2-34495-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human colorectal cancer shows strong nuclear positivity in tumor cells, but not in normal mucosa.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human lung cancer (squamous cell carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0018.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skin shows moderate to strong nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0019.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (CL2199) [NBP2-34495] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Immunohistochemistry-Paraffin-NBP2-34495-img0020.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Simple Western lane view shows a specific band for P53 in 0.2 mg/ml of Hek293T lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0009.jpg)
![Simple Western: p53 Antibody (CL2199) [NBP2-34495] - Electropherogram image(s) of corresponding Simple Western lane view. p53 antibody was used at 1:50 dilution on Hek293T lysate(s).](http://images.novusbio.com/fullsize/p53-Antibody-CL2199-Simple-Western-NBP2-34495-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/XRCC4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89463-img0012.jpg "Immunocytochemistry/Immunofluorescence: XRCC4 Antibody [NBP1-89463] - Staining of human cell line U-251 MG shows localization to nucleus. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Analysis in human testis and liver tissues using NBP1-89463 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/XRCC4-Antibody-Immunohistochemistry-Paraffin-NBP1-89463-img0016.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0009.jpg "Western Blot: AIF Antibody [NBP2-42496] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0008.jpg)
![Immunohistochemistry-Paraffin: AIF Antibody [NBP2-42496] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/AIF-Antibody-Immunohistochemistry-Paraffin-NBP2-42496-img0016.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0004.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of Mouse (A) and Rat (B) Kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0005.jpg)
![Western Blot: AIF Antibody [NBP2-42496] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Western-Blot-NBP2-42496-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: AIF Antibody [NBP2-42496] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/AIF-AIFM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42496-img0007.jpg)
![Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-FC82291-Western-Blot-NB600-1030-img0001.jpg "Western Blot: Nucleophosmin Antibody (FC82291) [NB600-1030] - Monoclonal Anti-B23 antibody produced in mouse")
![Immunofluorescence: NPM1 Antibody (FC82291) [NB600-1030] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 1:500. Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunofluorescence-NB600-1030-img0002.jpg)
![Immunoblotting: NPM1 Antibody (FC82291) [NB600-1030] - Cell line lysates were separated on SDS-PAGE and probed with 0.5 ug/mL Monoclonal Anti-B23 Clone: FC82291 Lanes 1. HeLa 2. JURKAT 3. COS7 4. NIH-3T3 5. RAT2 6. CHO 7. MDBK 8. MDCK](http://images.novusbio.com/fullsize/NPM1-Antibody-FC82291-Immunoblotting-NB600-1030-img0008.jpg)
![Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NB200-210-img0012.jpg "Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: p400 Antibody [NB200-210] - Detection of human p400 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NB200-210-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: p400 Antibody [NB200-210] - Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody NB200-210 (lot 2) used for IP at 6 ug per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-p400 antibody from Company B. For blotting immunoprecipitated p400, NB200-210 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/p400-Antibody-Immunoprecipitation-NB200-210-img0013.jpg)
![Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0018.jpg "Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.")
![Immunohistochemistry-Paraffin: EWSR1 Antibody [NB200-182] - Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (NB200-182). Detection: DAB](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-NB200-182-img0014.jpg)
![Western Blot: EWSR1 Antibody [NB200-182] - Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-182 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0016.jpg)
![Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunoprecipitation-NB200-182-img0015.jpg)
![Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Simple-Western-NB200-182-img0017.jpg)
