Hu,
Mu,
Rt,
Al,
Av,
Pl,
Ze,
RMSpecies Glossary
Reactivity: Hu, Mu, Rt, Al, Av, Pl, Ze, RM
WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, Mycoplasma
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, ChIP, Mycoplasma
Host: Rabbit Polyclonal
![Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p  0.05). n = 5–6/group. * P Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-91020242021994.jpg "Hydralazine enhances NRF2 signaling in SH-SY5Y cells. c Hydralazine reduced the interaction between NRF2 and KEAP1. Interactions were measured by reciprocal Co-IPs followed by western blot analysis. *p  0.05). n = 5–6/group. * P Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Nrf2 Antibody [NBP1-32822] - Nrf2 antibody [N2C2], Internal detects Nrf2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-910202420115018.jpg)
![Immunocytochemistry/ Immunofluorescence: Nrf2 Antibody [NBP1-32822] - Nrf2 antibody [N2C2], Internal detects Nrf2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nrf2 stained by Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-9102024202465.jpg)
![<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32822_rabbit-polyclonal-nrf2-antibody-western-blot-1411202518393121.jpg)
![NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 stained by NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32553_rabbit-polyclonal-hla-drb4-antibody-western-blot-26112025185022.jpg)
![Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (NBP1-32822) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp1-32462_rabbit-polyclonal-alpha-actinin-2-antibody-western-blot-26112025185017.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0014.jpg "Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunocytochemistry-Immunofluorescence-H00007337-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunohistochemistry-Paraffin-H00007337-M01-img0012.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0008.jpg)
![ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-ELISA-H00007337-M01-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Effect of E6AP silencing on the stability of HPV E6. (A)E6AP knockdown was confirmed by immunoblotting. (B) HEK293T cells (E6AP-null) were transfected with HPV16 E6 or HPV18 E6. (C) After 48 h the cells were harvested, & protein levels analyzed by immunoblotting. beta -actin acted as a control for transfection efficiency. (D) HEK293T cells (E6AP-null) were transfected with the indicated expression plasmids; after 36 h they were treated with 10 μM MG132 & 12 h later, harvested & subjected to immunoprecipitation (IP) with anti-myc-conjugated agarose beads. Polyubiquitinated HPV E6 was then detected for IP with an antibody against HA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31749782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007337-m01_mouse-monoclonal-e6ap-ube3a-antibody-2f6-310202415371978.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Nrf2-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00004780-M02-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2G7) [H00004780-M02] - Analysis of monoclonal antibody to NFE2L2 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: Nrf2 Antibody (2G7) [H00004780-M02] - Detection limit for recombinant GST tagged NFE2L2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Nrf2-Antibody-2G7-ELISA-H00004780-M02-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. Cardiac expression of endogenous antioxidant-related genes was significantly reduced after TAC. Representative blot of NRF2 and quantitative results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/h00004780-m02_mouse-monoclonal-nrf2-antibody-2g7-20920231412572.jpg)
![Western Blot: Nrf2 Antibody (2G7) [H00004780-M02] - EMPA treatment attenuated cardiomyocyte overproduction of reactive oxygen species (ROS) induced by TAC. (A) Left, Mitosox staining of cardiomyocytes in sham, sham + EMPA, TAC & TAC + EMPA groups & Right, quantitative results. (B) Representative blots of NRF-2 & HO-1, & quantitative results. (C) The relative mRNA levels of genes related to endogenous antioxidants. Results are expressed as mean ± SEM, n = 5–7, *p < 0.05 vs. corresponding sham group, †p < 0.05 vs. corresponding TAC vehicle group. One-way ANOVA & Tukey post hoc test. EMPA, empagliflozin; SEM, standard error of the mean; TAC, transverse aortic constriction; NRF-2, the nuclear factor erythroid 2–related factor 2; HO-1, heme oxygenase-1; GCLM, glutamate-cysteine ligase modifier subunit; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35647080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004780-m02_mouse-monoclonal-nrf2-antibody-2g7-310202415482245.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Western-Blot-NBP3-13682-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunocytochemistry-Immunofluorescence-NBP3-13682-img0004.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Human breast carcinoma. Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Nrf2-Antibody-HL1021-Immunohistochemistry-Paraffin-NBP3-13682-img0003.jpg)
![Western Blot: Nrf2 Antibody (HL1021) [NBP3-13682] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-910202420122022.jpg)
![Immunohistochemistry-Paraffin: Nrf2 Antibody (HL1021) [NBP3-13682] - Nrf2 antibody [HL1021] detects Nrf2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Nrf2 stained by Nrf2 antibody [HL1021] (NBP3-13682) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-91020242011413.jpg)
![Untreated (-) and treated (+) Rat2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-261120251850308.jpg)
![NRF2 antibody [HL1021] detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse salivary gland.NRF2 stained by NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-immunohistochemistry-paraffin-2611202518484812.jpg)
![Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1020] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-2611202518484817.jpg)
![Untreated (-) and treated (+) Neuro2A whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [HL1021] (NBP3-13682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13682_rabbit-nrf2-mab-hl1021-western-blot-261120251839624.jpg)
![Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.](http://images.novusbio.com/fullsize/SRC1-Antibody-1135-H4-Western-Blot-NB200-310-img0003.jpg "Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.")
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-240-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/Nrf2-p-Ser40-Antibody-SU0334-Western-Blot-NBP2-67465-img0009.jpg "Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Western blot analysis of Nrf2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Flow Cytometry: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Analysis of RIP3 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-flow-cytometry-2572024102417..jpg)
![Immunohistochemistry-Paraffin: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] -Rat kidney tissue using anti-RIP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-immunohistochemistry-paraffin-2572024102613..jpg)
![Western Blot: Nrf2 [p Ser40] Antibody (SU0334) [NBP2-67465] - Analysis of RIP3 on HT-29 cell lysates with Rabbit anti-RIP3 antibody at 1/1,000 dilution.Lysates/proteins at 10 ug/Lane.Predicted band size: 57 kDaObserved band size: 50 kDaExposure time: 2 minutes;12% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67465_rabbit-monoclonal-nrf2-p-ser40-antibody-su0334-western-blot-2572024102740..jpg)
![Immunocytochemistry/Immunofluorescence: SRC1 Antibody [NB300-541] - Immunolocalization of HeLa cells.](http://images.novusbio.com/fullsize/SRC1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-541-img0003.jpg "Immunocytochemistry/Immunofluorescence: SRC1 Antibody [NB300-541] - Immunolocalization of HeLa cells.")
![Immunohistochemistry-Frozen: SRC1 Antibody [NB300-541] - Staining of SRC-1 in the Dentate Gyrus region of the rat brain.](http://images.novusbio.com/fullsize/SRC1-Antibody-Immunohistochemistry-Frozen-NB300-541-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89092-img0007.jpg "Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody [NBP1-89092] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0013.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human testis shows moderate nuclear and cytoplasmic postivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0011.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody [NBP1-89092] - Staining of human skeletal muscle shows moderate nuclear and cytoplasmic postivity in myocytes.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Immunohistochemistry-Paraffin-NBP1-89092-img0012.jpg)
![Western Blot: E6AP/UBE3A Antibody [NBP1-89092] -Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/antibody/nbp1-89092_rabbit-polyclonal-e6ap-ube3a-antibody-western-blot-238202383532..jpg)
![ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Samples were spiked with high concentrations of Rat Nrf2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-08161_rat-nrf2-elisa-kit-colorimetric-132202416204022.jpg "ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Samples were spiked with high concentrations of Rat Nrf2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Nrf2 ELISA Kit (Colorimetric) [NBP3-08161] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-08161_rat-nrf2-elisa-kit-colorimetric-432024915832.jpg)
![Western Blot: SRC1 Antibody [NB100-312] - Detection of Human and Mouse SRC1 by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), and (50 ug) from HeLa, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody NB100-312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SRC1-Antibody-Western-Blot-NB100-312-img0010.jpg "<b>Biological Strategies Validation. </b>Western Blot: SRC1 Antibody [NB100-312] - Detection of Human and Mouse SRC1 by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), and (50 ug) from HeLa, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody NB100-312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SRC1 Antibody [NB100-312] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/SRC1-Antibody-Immunohistochemistry-NB100-312-img0008.jpg)
![Western Blot: SRC1 Antibody [NB100-312] - Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 ug/10 mg extract.](http://images.novusbio.com/fullsize/SRC1-Antibody-Western-Blot-NB100-312-img0002.jpg)
![Immunoprecipitation: SRC1 Antibody [NB100-312] - Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody NB100-312 (lot NB100-312-2) used for IP at 6 ug per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody NB100-312-1. For blotting immunoprecipitated SRC1, NB100-312 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SRC1-Antibody-Immunoprecipitation-NB100-312-img0011.jpg)
![Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21580.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-5720231721512.jpg "Immunohistochemistry: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NBP3-21580.")
![Western Blot: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Western blot analysis using NBP3-21580 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-67202310627.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf2 [p Ser 40] Antibody (SR2277) [NBP3-21580] - Immunofluorescent analysis of HepG2 cells, using NBP3-21580.](http://images.novusbio.com/fullsize/nbp3-21580_rabbit-nrf2-p-ser-40-mab-sr2277-6720231051260.jpg)