| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71615 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73200 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61702-0.025ml | |||
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0016.jpg "Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunocytochemistry-Immunofluorescence-H00000196-M02-img0012.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0008.jpg)
![Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Western-Blot-H00000196-M02-img0014.jpg)
![Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-Immunoprecipitation-H00000196-M02-img0013.jpg)
![ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/AHR-Antibody-3B12-ELISA-H00000196-M02-img0015.jpg)
![Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Western-Blot-NBP2-76420-img0001.jpg "Western Blot: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), Jurkat, TCMK-1, and NIH 3T3 lysate. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF by Simple Western™. Samples: Whole cell lysate (2.0 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311310.jpg)
![Simple Western: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] (NBP2-76420) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image validated internally.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-simple-western-303202311923.jpg)
![Immunocytochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE MCF-7 cells by ICC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunocytochemistry-NBP2-76420-img0003.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of mouse CTCF in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0004.jpg)
![Immunohistochemistry: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunohistochemistry-NBP2-76420-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CTCF Antibody (BLR041F) [NBP2-76420] - Detection of human CTCF by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CTCF recombinant monoclonal [BLR041F] (NBP2-76420) and rabbit anti-CTCF (NB500-177).](http://images.novusbio.com/fullsize/CTCF-Antibody-BLR041F-Immunoprecipitation-NBP2-76420-img0005.jpg)
![Flow Cytometry-CTCF Antibody (BLR041F) [NBP2-76420] -Rabbit anti-CTCF recombinant monoclonal antibody [BLR041F] or isotype control (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.](http://images.novusbio.com/fullsize/nbp2-76420_rabbit-monoclonal-ctcf-antibody-blr041f-flow-cytometry-287202312246..jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0013.jpg "Western Blot: AHR Antibody (RPT1) [NB300-530] - Analysis of 20ug of HepG2, U2OS, and K562 whole cell lysates and 5uL of PageRuler Plus Prestained Protein Ladder per well.")
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0010.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:100.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0012.jpg)
![Western Blot: AHR Antibody (RPT1) [NB300-530] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Western-Blot-NB300-530-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody (RPT1) [NB300-530] - Analysis of Aryl Hydrocarbon Receptor in MCF-7 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Aryl Hydrocarbon Receptor monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Aryl Hydrocarbon Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunocytochemistry-Immunofluorescence-NB300-530-img0009.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody (RPT1) [NB300-530] - Normal biopsies of deparaffinized Human skeletal muscle tissue at a dilution of 1:20.](http://images.novusbio.com/fullsize/AHR-Antibody-RPT1-Immunohistochemistry-Paraffin-NB300-530-img0011.jpg)
![Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Western-Blot-NBP2-45872-img0011.jpg "Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.")
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0010.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0001.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0002.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0003.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0004.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0005.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0007.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0008.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0009.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0010.jpg "Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunocytochemistry-Immunofluorescence-NBP2-02627-img0009.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0008.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0002.jpg)
![Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Western-Blot-NBP2-02627-img0003.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0004.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0005.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0006.jpg)
![Immunohistochemistry-Paraffin: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Immunohistochemistry-Paraffin-NBP2-02627-img0007.jpg)
![Flow Cytometry: IGF2BP2 Antibody (3F9) [NBP2-02627] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IGF2BP2-Antibody-3F9-Flow-Cytometry-NBP2-02627-img0001.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0018.jpg "Western Blot: p53 Antibody (5E2) [NBP2-00723] - Analysis of extracts (35ug) from 9 different cell lines by using anti-p53 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: p53 Antibody (5E2) [NBP2-00723] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunocytochemistry-Immunofluorescence-NBP2-00723-img0013.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0016.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-p53 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0012.jpg)
![Western Blot: p53 Antibody (5E2) [NBP2-00723] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY p53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-p53.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Western-Blot-NBP2-00723-img0017.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0014.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (5E2) [NBP2-00723] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-p53 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Immunohistochemistry-Paraffin-NBP2-00723-img0015.jpg)
![Flow Cytometry: p53 Antibody (5E2) [NBP2-00723] - Analysis of Hela cells, using anti-p53 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Flow-Cytometry-NBP2-00723-img0011.jpg)
![Simple Western: p53 Antibody (5E2) [NBP2-00723] - Simple Western lane view shows a specific band for p53 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-5E2-Simple-Western-NBP2-00723-img0010.jpg)
![Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against human BCL11B (AA: 1-150) recombinant protein. (Expected MW is 42.3 kDa)](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Western-Blot-NBP2-61702-img0006.jpg "Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against human BCL11B (AA: 1-150) recombinant protein. (Expected MW is 42.3 kDa)")
![Immunocytochemistry/Immunofluorescence: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunocytochemistry-Immunofluorescence-NBP2-61702-img0003.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of stomach cancer tissues using BCL11B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunohistochemistry-NBP2-61702-img0005.jpg)
![Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Jurkat cells using BCL11B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Flow-Cytometry-NBP2-61702-img0010.jpg)
![Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against HEK293 (1) and BCL11B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Western-Blot-NBP2-61702-img0007.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of colon cancer tissues using BCL11B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Immunohistochemistry-NBP2-61702-img0004.jpg)
![Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-Flow-Cytometry-NBP2-61702-img0002.jpg)
![ELISA: BCL11B Antibody (1F8G8) [NBP2-61702] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BCL11B-Antibody-1F8G8-ELISA-NBP2-61702-img0001.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0002.jpg "Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71615-img0005.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0009.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0004.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0001.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0006.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0007.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0008.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0010.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0003.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NB100-81909-img0010.jpg "Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - ICC/IF showing estrogen receptor alpha (green) in human cervical stromal cells. Nuclei are stained with DAPI (blue). Antibody at 1:200 in 3% BSA. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81909-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-81909 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NB100-81909-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of rat-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241523500.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-2342024155515.jpg)
![Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of Hela cells.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414521010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of lysates from 293 cells, using NB100-81909. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414462211.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Human-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241521260.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0011.jpg "Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Western blot analysis of E2F-1 on NIH/3T3 cell lysates with Rabbit anti-E2F1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 47 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0004.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0012.jpg)
![Flow Cytometry: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Flow-Cytometry-NBP2-67899-img0001.jpg)
![Western Blot: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of E2F1 on HepG2 cells lysates using anti-E2F1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Western-Blot-NBP2-67899-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Staining E2F1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunocytochemistry-Immunofluorescence-NBP2-67899-img0003.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0005.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Analysis of paraffin-embedded mouse pancreas tissue using anti-E2F1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0006.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0008.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0009.jpg)
![Immunohistochemistry-Paraffin: E2F-1 Antibody (JJ092-02) [NBP2-67899] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-E2F-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/E2F-1-Antibody-JJ092-02-Immunohistochemistry-Paraffin-NBP2-67899-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0005.jpg "Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunocytochemistry-Immunofluorescence-NBP2-67777-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded mouse bone tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysateLane 2: U937 cell lysate Lane 3: HL-60 cell lysate](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0004.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0008.jpg)
![Western Blot: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB100-56374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Carm1-Antibody-Knockout-Validated-NB100-56374-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB100-56374] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB100-56374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Carm1 Antibody [NB100-56374] - Analysis of PRMT4 in cell lysates from HeLa cells using this antibody. 5 ug/ml. Twenty ug of HeLa cell lysate was loaded per well of mini gel.](http://images.novusbio.com/fullsize/Carm1-Antibody-Western-Blot-NB100-56374-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Carm1 Antibody [NB100-56374] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Carm1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56374-img0005.jpg)
![Immunohistochemistry-Paraffin: Carm1 Antibody [NB100-56374] - Analysis of a FFPE tissue section of the human breast carcinoma using 1:200 dilution of Carm1 antibody (NB100-56374). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated nuclear and cytoplasmic staining.](http://images.novusbio.com/fullsize/Carm1-Antibody-Immunohistochemistry-Paraffin-NB100-56374-img0004.jpg)