| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77461SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47254SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89104-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24755SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44496 | |||
| BSA Free | NBP2-52887-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89842-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22391SS | |||
| Azide and BSA Free | NBP2-80934 | |||
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31388010/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0009.jpg "Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Vascularization of mineralized cell-laden collagen and interaction with prostate cancer cells. HUVECs formed endothelial networks that were supported by SMA-expressing hMSCs (scale bar: 50m) and were also positive for CD31 (scale bar: 400m). The remainder of hMSCs expressed RUNX2 as a marker for osteogenic differentiation (scale bar: 50m). Rapid fabrication of vascularized and innervated cell-laden bone models with biomimetic intrafibrillar collagen mineralization. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31388010/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77461] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with RUNX2/CBFA1 Antibody (NBP1-77461) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77461-img0010.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - RUNX2/CBFA1 Antibody [NBP1-77461] - Characterization of TLC cultured in chondrogenic pellets. Gene expression of TLC cultured four weeks in chondrogenic pellets or in cell monolayer in control medium: levels of expression of osteoblast markers (1), chondrocyte markers (2) and tenocyte markers (3) (N = 3) assessed by qPCR.* =0.05. Rotator Cuff Tenocytes Differentiate into Hypertrophic Chondrocyte-Like Cells to Produce Calcium Deposits in an Alkaline Phosphatase-Dependent Manner. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31561454/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-77461-img0008.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77461] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-NBP1-77461-img0006.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody - BSA Free [NBP1-77461] - Histological characterization of rotator cuff calcifications (N = 5). (A) Calcific deposits assessed by micro-computed tomography. (B) HE (hematoxylin & eosin) staining of decalcified samples (1 image for each patient). (C) Von Kossa staining on not decalcified samples & characterization of the fibrocartilaginous area with alcian blue staining, SOFG (Safranin O/Fast Green) staining, & immunohistochemical staining for COL2 (brown) with Gill hematoxylin counterstain. (D,E) Representative immunohistochemical staining for ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), TNAP (tissue non-specific alkaline phosphatase), Runx2, Sox9, Col10 & CD31 (brown). TF: tendon fibers; CD: calcium deposits, FM: fibrocartilaginous metaplasia; OM: osseous metaplasia; CT: connective tissue; V: vessels. Scale bar = 200 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31561454), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77461_rabbit-polyclonal-runx2-cbfa1-antibody-310202416154628.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0012.jpg "Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with conjugated to DyLight 488 (NBP1-47254G) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - The protein expression levels in QF muscle. Representative Western blot images. n = 14-15 animals/group. *=vs. LFsed (P 0.1). Black bars = sedentary, grey bars = running. Image collected and cropped by CiteAb from the following publication (//nutritionandmetabolism.biomedcentral.com/articles/10.1186/1743-7075-9-53), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0011.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. The different cell types in the cellular epidermis as well as the blood vessels in the dermal layer showed positivity for ERR alpha, and the staining was found to be more intense in the nuclei compared to cytoplasm. (10X Magnification)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0013.jpg "Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of RUNX2 expression in transfected 293T cell line by RUNX2 monoclonal antibody (M01), clone 1D8.Lane 1: RUNX2 transfected lysate (Predicted MW: 60.28 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunocytochemistry-Immunofluorescence-H00000860-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Immunohistochemistry-Paraffin-H00000860-M01-img0010.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in SJCRH30 ( Cat # L027V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0001.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Western Blot analysis of RUNX2 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - RUNX2 monoclonal antibody (M01), clone 1D8. Analysis of RUNX2 expression in Jurkat.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Western-Blot-H00000860-M01-img0012.jpg)
![Sandwich ELISA: RUNX2/CBFA1 Antibody (1D8) [H00000860-M01] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-1D8-Sandwich-ELISA-H00000860-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining in human tonsil and liver tissues . Corresponding RUNX2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89104-img0012.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human tonsil shows moderate to strong nuclear positivity in a subset lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0008.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human rectum shows moderate to strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0009.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody [NBP1-89104] - Staining of human salivary gland shows moderate to strong nuclear positivity in ductal cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-Paraffin-NBP1-89104-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: RUNX2/CBFA1 Antibody [NBP1-89104] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Knockdown-Validated-NBP1-89104-img0014.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Western-Blot-H00000860-M06-img0001.jpg "Western Blot: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Western Blot analysis of RUNX2 expression in Hela NE ( Cat # L012V1 ).")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on U-2 OS cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Analysis of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunohistochemistry-Paraffin-H00000860-M06-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell . [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-Immunocytochemistry-Immunofluorescence-H00000860-M06-img0003.jpg)
![ELISA: RUNX2/CBFA1 Antibody (3F5) [H00000860-M06] - Detection limit for recombinant GST tagged RUNX2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-3F5-ELISA-H00000860-M06-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Western-Blot-NBP2-24755-img0002.jpg "Western Blot: RUNX2/CBFA1 Antibody [NBP2-24755] - Analysis of human RUNX2 in Saos-2 cell lysate in the 1) absence and 2) presence of immunizing peptide using RUNX2 antibody at 2 ug/mL.")
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0005.jpg "Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunocytochemistry-Immunofluorescence-NBP2-67777-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded mouse bone tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysateLane 2: U937 cell lysate Lane 3: HL-60 cell lysate](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0004.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0008.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-NBP1-77462-img0005.jpg "Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77462] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77462-img0006.jpg)
![Flow Cytometry: RUNX2/CBFA1 Antibody [NBP1-77462] - An intracellular stain was performed on HeLa cells with RUNX2/CBFA1 Antibody NBP1-77462AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Flow-Cytometry-NBP1-77462-img0007.jpg)
![Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of C2C12 cells, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RCOR1-CoREST-Antibody-9B3R7-Western-Blot-NBP3-16225-img0005.jpg "Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of C2C12 cells, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of various cell lines, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RCOR1-CoREST-Antibody-9B3R7-Western-Blot-NBP3-16225-img0004.jpg)
![Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of 300ug extracts of THP-1 cells using 3ug RCOR1/CoREST antibody (NBP3-16225). Western blot was performed from the immunoprecipitate using RCOR1/CoREST antibody (NBP3-16225) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/RCOR1-CoREST-Antibody-9B3R7-Immunoprecipitation-NBP3-16225-img0006.jpg)
![Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RCOR1/CoREST antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202511392249.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-522025113893.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202511374143.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202511374119.jpg)
![Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [RCOR1/CoREST] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RCOR1/CoREST antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202517223973.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [RCOR1/CoREST] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202517152750.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [RCOR1/CoREST] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202517192930.jpg)
![Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [RCOR1/CoREST] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16225_rabbit-rcor1-corest-mab-9b3r7-52202517273556.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RUNX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of RH-30 cells using the Anti-RUNX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunocytochemistry-Immunofluorescence-NBP2-52887-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells outside the reaction centrum.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0004.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Western-Blot-NBP2-52887-img0005.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human rectum shows nuclear positivty in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human small intestine shows nuclear immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (CL0232) [NBP2-52887] - Staining of human testis shows absence of staining (negative control).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-CL0232-Immunohistochemistry-Paraffin-NBP2-52887-img0003.jpg)
![Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in human cell lines U2OS and HEK293 using anti-FOSL2 antibody. Corresponding FOSL2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/FRA2-Antibody-Western-Blot-NBP1-89842-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in human cell lines U2OS and HEK293 using anti-FOSL2 antibody. Corresponding FOSL2 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FRA2 Antibody [NBP1-89842] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89842-img0010.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human stomach shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0019.jpg)
![Western Blot: FRA2 Antibody [NBP1-89842] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FRA2-Antibody-Western-Blot-NBP1-89842-img0007.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human cervix shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0016.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0017.jpg)
![Immunohistochemistry-Paraffin: FRA2 Antibody [NBP1-89842] - Staining of human skin shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/FRA2-Antibody-Immunohistochemistry-Paraffin-NBP1-89842-img0018.jpg)
![Western Blot: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Western-Blot-NBP2-22391-img0008.jpg "Western Blot: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Staining of normal human spleen tissue stained with Runx3 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Immunohistochemistry-Paraffin-NBP2-22391-img0007.jpg)
![Flow Cytometry: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Intracellular flow analysis of human lymphocytes probed with Runx3 antibody (red) and isotype control (green) at 0.25 ug/10^6 cells.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Flow-Cytometry-NBP2-22391-img0006.jpg)