| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62555-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29626SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87466-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38790-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87758-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90010-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76454SS | |||
| Azide and BSA Free | NBP2-80767 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-220SS | |||
![Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Western-Blot-NBP2-62555-img0003.jpg "Western Blot: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Western Blot using anti-p53 antibody PAb421 A431 cell nuclear (A) and cytoplasmic (B) extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of PAb421 (NBP2-62555) at 0.1 ug/ml before detection by an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for p53 is 43.7 kDa, though due to the high number of proline residues in this protein runs at a size of ~53kDa (c.f. Ziemer et al., PMID: 7107651). NBP2-62555 successfully detected both human nuclear and cytoplasmic p53.")
![Flow Cytometry: p53 Antibody (PAb421) - Chimeric [NBP2-62555] - Flow-cytometry using the anti-p53 antibody PAb421. Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of PAb421 (NBP2-62555, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/p53-Antibody-PAb421-Chimeric-Flow-Cytometry-NBP2-62555-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: p53 Antibody (PAb421) - Chimeric - Azide and BSA Free [NBP2-62555] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton and stained with NBP2-62555 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62555, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62555_rabbit-monoclonal-p53-antibody-pab421-chimeric-7420252226522.png)
![Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.](http://images.novusbio.com/fullsize/SNAIL-Antibody-Western-Blot-NBP2-29626-img0001.jpg "Western Blot: SNAIL Antibody [NBP2-29626] - Western blot analysis of SNAIL in HeLa whole cell extract.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of dissociated rat hippocampal culture. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-NBP1-87466-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0008.jpg)
![Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Western-Blot-NB200-335-img0006.jpg "Western Blot: NCOA6 Antibody [NB200-335] - Detection of Human ASC2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody NB200-335 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: NCOA6 Antibody [NB200-335] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunohistochemistry-Paraffin-NB200-335-img0008.jpg)
![Immunoprecipitation: NCOA6 Antibody [NB200-335] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody used for IP at 6 ug per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-ASC2 antibody. For blotting immunoprecipitated ASC2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NCOA6-Antibody-Immunoprecipitation-NB200-335-img0013.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).](http://images.novusbio.com/fullsize/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (4C4) [H00006935-M01] - Analysis of monoclonal antibody to ZEB1 on U-2 OS cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZEB1-Antibody-4C4-Immunocytochemistry-Immunofluorescence-H00006935-M01-img0003.jpg)
![ELISA: ZEB1 Antibody (4C4) [H00006935-M01] - Detection limit for recombinant GST tagged ZEB1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-4C4-ELISA-H00006935-M01-img0006.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0014.jpg "Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.")
![Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunocytochemistry-Immunofluorescence-H00007337-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Immunohistochemistry-Paraffin-H00007337-M01-img0012.jpg)
![Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-Western-Blot-H00007337-M01-img0008.jpg)
![ELISA: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Detection limit for recombinant GST tagged UBE3A is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-2F6-ELISA-H00007337-M01-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Effect of E6AP silencing on the stability of HPV E6. (A)E6AP knockdown was confirmed by immunoblotting. (B) HEK293T cells (E6AP-null) were transfected with HPV16 E6 or HPV18 E6. (C) After 48 h the cells were harvested, & protein levels analyzed by immunoblotting. beta -actin acted as a control for transfection efficiency. (D) HEK293T cells (E6AP-null) were transfected with the indicated expression plasmids; after 36 h they were treated with 10 μM MG132 & 12 h later, harvested & subjected to immunoprecipitation (IP) with anti-myc-conjugated agarose beads. Polyubiquitinated HPV E6 was then detected for IP with an antibody against HA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31749782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007337-m01_mouse-monoclonal-e6ap-ube3a-antibody-2f6-310202415371978.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Western-Blot-NBP1-87758-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87758-img0008.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Western-Blot-NBP1-87758-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human colon shows moderate cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human lymph node shows strong cytoplasmic positivity in germinal- and non-germinal center cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0015.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/NFkB-p105-p50-Antibody-Immunohistochemistry-Paraffin-NBP1-87758-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: TFII-I Antibody [NBP1-90010] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TFII-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90010-img0008.jpg "Immunocytochemistry/Immunofluorescence: TFII-I Antibody [NBP1-90010] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TFII-I Antibody [NBP1-90010] - Staining of human stomach shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/TFII-I-Antibody-Immunohistochemistry-Paraffin-NBP1-90010-img0002.jpg)
![Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Total protein from human U87 cells treated for 4 hours with and without 100 uM cobalt chloride (CoCl2) and under normoxic and hypoxic conditions, was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HIF-2 alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Human MDA MB 231 cells. 50 ug cell lysate per lane. 10% SDS-PAGE. HIF-2 alpha antibody at 1:1000 in 5% milk. HRP-conjugated donkey anti-rabbit IgG polyclonal secondary antibody at 1:2000 in 5% milk. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Western-Blot-NBP2-76454-img0003.jpg)
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2444A) [NBP2-76454] - Analysis of an FFPE tissue section of the human renal carcinoma using 1:100 dilution of HIF-2 alpha antibody (NBP2-76454). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the nuclear with minor cytoplasmic staining.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2444A-Immunohistochemistry-Paraffin-NBP2-76454-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TAZ/WWTR1 Antibody [NB600-220] - Western blot analysis of TAZ in MDCK cells using NB600-220 at 1:250 dilution.](http://images.novusbio.com/fullsize/TAZ-WWTR1-Antibody-Western-Blot-NB600-220-img0004.jpg)
![Western Blot: TAZ/WWTR1 Antibody - BSA Free [NB600-220] - Pharmacologic targeting the beta 1-integrin-FAK-YAP1/TAZ signaling suppresses cancer spheroids & VASP is required for RhoA-mediated YAP1/TAZ dephosphorylation. a Upper, 3D cancer spheroids were harvested for WB for CTGF. VASP knockdown reduced CTGF protein levels in KM12L4 & L3.6 cells. *p 50 per group. Bar: 100 µm. d HCT116 cells expressing LacZ (control) or RhoAQ63L were seeded on Matrigel to induce cancer spheroids. Overexpression of RhoAQ63L increased the size of cancer spheroids. *p 50 per group. e Control & VASP knockdown cancer spheroids were harvested for regular WB & Phos-tagTM gel-based WB. RhoAQ63L increased YAP1/TAZ protein levels & YAP1/TAZ dephosphorylation in control cells & these RhoAQ63L effects on YAP1/TAZ were abrogated by VASP knockdown. Densitometry data are shown on the right. *p 50 per group; Bar, 100 µm. b VASP knockdown in two PDAC cancer cell lines significantly reduced the size of cancer spheroids. *p 50 per group; Bar, 100 µm. c Cancer spheroids were harvested for WB. VASP knockdown in 4 cell lines consistently reduced phosphorylation of FAK & Src & protein levels of YAP1/TAZ. The effect of VASP knockdown on phosphorylation of Akt or Erk was not consistent & it did not influence the total FAK & Src protein levels. Quantitative data of HCT116 are shown on the bottom. *p < 0.05, by t-test, n = 3. Samples derived from the same experiment & gels/blots were processed in parallel. Error bar: S.D Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29872721), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-220_rabbit-polyclonal-taz-wwtr1-antibody-310202416163758.jpg)