| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-22530-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1833SS | |||
| Azide and BSA Free | NBP2-80986 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77276SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25142SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2080SS | |||
| Azide and BSA Free | NBP2-80733 | |||
![Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Western-Blot-NBP2-22530-img0002.jpg "Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.")
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 (green) showing staining in the membrane of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Immunocytochemistry-Immunofluorescence-NBP2-22530-img0004.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0006.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0003.jpg)
![Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/CD44-Antibody-Hermes-1-Flow-Cytometry-NBP2-22530-img0005.jpg)
![Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 2 independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-16168-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPL22-Antibody-Western-Blot-NBP1-06069-img0007.jpg "Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 2 independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-16168-x) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0006.jpg)
![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0003.jpg)
![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0004.jpg)
![Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RPL22-Antibody-Immunohistochemistry-Paraffin-NBP1-06069-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0006.jpg "Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - The effect of N-BLR knockdown on invasion by specific siRNAs. E-cadherin, vimentin, and ZEB1 were identified in vitro by immunofluorescence with specific antibodies. Immunofluorescence signal of E-cadherin (green color) was markedly increased in both clones. The ZEB1 signal was present in cells with empty vector (green color) but not in clones #3-1 and #4-7. Blue color indicate nuclei. Single green, blue, and merged channel images of ZEB1 are reported in Additional file 3: Figure S9B. Image collected and cropped by CiteAb from the following publication (//genomebiology.biomedcentral.com/articles/10.1186/s13059-017-1224-0) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in a frozen tissue section of human colon. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunohistochemistry-Frozen-NBP1-97670-img0005.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - An intracellular stain was performed on Jurkat cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Flow-Cytometry-NBP1-97670-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of glomeruli and connetive tissue in human kidney with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody (V9) [NBP1-97670] - Strong specific staining of connective tissue cells in swine colon with in a 1:200 dilution. No reactivity in epithelial cells.](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Immunocytochemistry-Immunofluorescence-NBP1-97670-img0004.jpg)
![Flow Cytometry: Vimentin Antibody (V9) [NBP1-97670] - Analysis of PE conjugate of NBP1-97670. An intracellular stain was performed on THP-1 cells with Vimentin antibody (V9) NBP1-97670PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% s](http://images.novusbio.com/fullsize/Vimentin-Antibody-V9-Flow-Cytometry-NBP1-97670-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in human tonsil. NBP1-97670 was diluted to 0.5ug/mL and was left on sections for 30m at room temperature. Secondary antibody was Horse-Anti Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023111714..jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in horse intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on slides for 30m at room temperature. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023112025..jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in feline intestine. NBP1-97670 was used at a concentration of 0.5ug/mL. Secondary antibody was Horse Anti-Mouse HRP Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023112626.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (V9) [NBP1-97670] - Vimentin immunoreactivity in canine intestine. NBP1-97670 was used at a concentration of 0.5ug/mL and was left on tissue sections for 30m at room temperature. Secondary was Horse Anti-Mouse Veterinary Reagent. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1462023115136..jpg)
![Immunohistochemistry-Paraffin: Mouse Monoclonal Vimentin Antibody (V9) [NBP1-97670] - Vimentin was stained in human FFPE tonsil tissue. HIER antigen retrieval at pH 9 for 20min was performed. Concentration used: 1:100. AF750 conjugated version of the antibody was used (Catalog # NBP1-97670AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-97670_mouse-monoclonal-vimentin-antibody-v9-immunohistochemistry-paraffin-1122023155957..jpg)
![Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/CDT2-Antibody-Western-Blot-NB100-40840-img0009.jpg "Western Blot: CDT2 Antibody [NB100-40840] - Whole cell lysate (15 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DTL/CDT2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunohistochemistry-Paraffin-NB100-40840-img0011.jpg)
![Immunohistochemistry-Paraffin: CDT2 Antibody [NB100-40840] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DTL/CDT2 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunohistochemistry-Paraffin-NB100-40840-img0010.jpg)
![Immunoprecipitation: CDT2 Antibody [NB100-40840] - Detection of human DTL/CDT2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DTL/CDT2 antibody NB100-40840 (lot NB100-40840-3) used for IP at 6 ug per reaction. DTL/CDT2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-40840-2). For blotting immunoprecipitated DTL/CDT2, NB100-40840 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/CDT2-Antibody-Immunoprecipitation-NB100-40840-img0008.jpg)
![Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.](http://images.novusbio.com/fullsize/TRA-1-81-Antibody-TRA-1-81-Western-Blot-NB100-1833-img0006.jpg "Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane.](http://images.novusbio.com/fullsize/TRA-1-81-Antibody-TRA-1-81-Immunocytochemistry-Immunofluorescence-NB100-1833-img0012.jpg)
![Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.](http://images.novusbio.com/fullsize/TRA-1-81-Antibody-TRA-1-81-Flow-Cytometry-NB100-1833-img0010.jpg)
![Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Western-Blot-NB100-288-img0018.jpg "Western Blot: MCM2 Antibody [NB100-288] - Detection of human and mouse MCM2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: MCM2 Antibody [NB100-288] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunohistochemistry-NB100-288-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: MCM2 Antibody [NB100-288] - Detection of human MCM2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MCM2 antibody NB100-288 used for IP at 3 ug per reaction. MCM2 was also immunoprecipitated by rabbit anti-Phospho MCM2 (S108) antibody NB100-639. For blotting immunoprecipitated MCM2, NB100-288 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/MCM2-Antibody-Immunoprecipitation-NB100-288-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Integrin-beta-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16974-img0003.jpg "Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody [NBP2-16974] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-16974] - Human colon carcinoma, using Integrin beta 1 / CD29 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15minof paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Integrin-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16974-img0002.jpg)
![Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1/CD29 antibody (NBP2-16974) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16974_rabbit-polyclonal-integrin-beta-1-cd29-antibody-91020242011496.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Integrin beta 1/CD29 Antibody [NBP2-16974] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Integrin beta 1 / CD29 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16974_rabbit-polyclonal-integrin-beta-1-cd29-antibody-1610202419414566.jpg)
![Western Blot: PD-1 Antibody [NBP1-77276] - THP-1 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/PD-1-Antibody-Western-Blot-NBP1-77276-img0005.jpg "Western Blot: PD-1 Antibody [NBP1-77276] - THP-1 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: PD-1 Antibody [NBP1-77276] - Human Brain tissue with PD-1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/PD-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77276-img0009.jpg)
![Immunohistochemistry: PD-1 Antibody [NBP1-77276] - Staining of human tonsil tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/PD-1-Antibody-Immunohistochemistry-NBP1-77276-img0008.jpg)
![Immunohistochemistry-Paraffin: PD-1 Antibody [NBP1-77276] - Human brain tissue with PD-1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/PD-1-Antibody-Immunohistochemistry-Paraffin-NBP1-77276-img0004.jpg)
![In vitro inhibition by the CH-4 analog of the PD-1/PD-L1 interaction. (A) HEK293 cells (left-hand panel) and Jurkat cells (right-hand panel) were treated with CH-4.7 or CH-4.9 to test cytotoxicity. Cells were seeded into 96-well plates at a density of 7 × 103/well and incubated overnight, then treated with increasing concentrations of CH-4.7 or CH-4.9 (0, 10, 20, 40, 80, or 100 uM) for 48 h. At 48 h, cell cytotoxicity WST-1 assays were performed. The x-axis indicates treatment concentrations, while the y-axis indicates the percentage of cell viability (each absolute absorbance value [abs. 450 nm–630 nm] was normalized with the controls). (B) The capacities of CH-4.7 and CH-4.9 to inhibit the PD-1/PD-L1 interaction were tested by the flow cytometry assay. The graphs present the mean +/- SD (standard deviation) values from at least three independent experiments. (C) CH-4.7 cytotoxicity in KG-1 cells. (D) Flow cytometry determined binding of the Ni–NTA-l-labeled sPD-L1 (PD-L1-Atto) complex to KG-1 cells expressing PD-1. Cell staining (FITC-subset) was blocked in the presence of CH-4.7. The experimental groups are indicated as (a) KG-1/PBS, (b) KG-1/Atto dye, (c) KG-1/sPD-L1/Atto dye, (d) KG-1/sPD-L1/Atto dye/CH-4 (10 uM), (e) KG-1/sPD-L1/Atto dye/CH-4.7 (20 uM). (E) The cell staining data from (D) are normalized and quantified as relative MFI values. (F) At 48 h, PD-1 protein (20 ug/well) expression was determined by Western blotting. The bar graphs present the mean +/- SD (standard deviation) values from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34996924), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77276_rabbit-polyclonal-pd-1-antibody-western-blot-132202613302222.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0012.jpg "Western Blot: Actin Antibody (5J11) [NBP2-25142] - NBP2-25142 was used to detect GST fusion proteins expressing each of the six human actin proteins. GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with NBP2-25142. The antibody stained all six proteins indicating that it is a panspecific actin antibody.")
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - Beta actin was detected in NIH-3T3 cells fixed with methanol using mouse anti-mouse beta-Actin monoclonal antibody (NBP2-25142) at 1:2700 dilution. Cells were stained using Northern Lights 557 conjugated anti-mouse secondary antibody (NL007) and counterstained with DAPI.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0010.jpg)
![Flow Cytometry: Actin Antibody (5J11) [NBP2-25142] - analysis of HeLa cells using mouse Monoclonal Actin antibody (Orange) and Isotype control Antibody (Blue).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Flow-Cytometry-NBP2-25142-img0008.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of HeLa, A431, K562, MCF-7, NIH-3T3, C6, and Rat-2 cell lines.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0007.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.2 ug/mL mouse anti-Actin monoclonal (NBP2-25142, Novus Biologicals), followed by 1:2000 dilution of HRP- conjugated secondary antibody, donkey anti-mouse IgG (HAF018, R&D Systems).](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0009.jpg)
![Western Blot: Actin Antibody (5J11) [NBP2-25142] - Analysis of tissue and cell lysates probed with mouse mAb against actin, NBP2-25142, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells, in green.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Western-Blot-NBP2-25142-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Actin Antibody (5J11) [NBP2-25142] - HeLa cells stained with NBP2-25142 (green) and also with the chicken polyclonal antibody to Vimentin (NB300-223, red). The Actin antibody stains the submembranous Actin rich cytoskeleton and also stress fibers, bundles of Actin associated with adhesion sites. The Vimentin antibody stains a quite different cytoskeletal network, the intermediate filaments. The blue stain reveals DNA in the nuclei of these cells.](http://images.novusbio.com/fullsize/Actin-Antibody-5J11-Immunocytochemistry-Immunofluorescence-NBP2-25142-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0011.jpg "Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] conjugated to Alexa Fluor 488 (NB100-2080AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FGFR1-Antibody-M17D10-Immunocytochemistry-Immunofluorescence-NB100-2080-img0012.jpg)
![Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Western-Blot-NB100-2080-img0004.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/ml concentration. Specific membrane-cytoplasmic immunopositivity of FGF R1 protein was observed in SCC cells whereas the nuclei of SCC cells as well as the tumor stroma was largely negative for this protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0008.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/mL) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0010.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human normal skin using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. Cells of the epidermal layer showed a very strong membrane-cytoplasmic staining with relatively weak nuclear immunopositivity for for this protein. The stratum basale / bottom layer of keratinocytes in the epidermis showed a membrane-cytoplasmic stainin for FGF R1.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0006.jpg)
![Immunohistochemistry-Paraffin: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FFPE tissue section of human liver using mouse monoclonal FGF R1 antibody (clone M17D10) at 5 ug/mL. The hepatocytes depicted an expected and specific membrane-cytoplasmic-nuclear immunostaining of FGF R1 protein.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Immunohistochemistry-Paraffin-NB100-2080-img0007.jpg)
![Flow Cytometry: FGF R1 Antibody (M17D10) [NB100-2080] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2080, red) at 1:1000 dilution. Detected with FITC conjugated goat anti-mouse IgG1 isotype control (green). Two distinct samples shown.](http://images.novusbio.com/fullsize/FGF-R1-Antibody-M17D10-Flow-Cytometry-NB100-2080-img0009.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0010.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48?h, cells were immunostained for midbody markers (green), Beta-tubulin (red), and DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-0561-6) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Immunocytochemistry-Immunofluorescence-H00029127-M01-img0011.jpg)
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Analysis of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Immunohistochemistry-Paraffin-H00029127-M01-img0007.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Western Blot analysis of RACGAP1 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0003.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in 293.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0009.jpg)
![ELISA: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-ELISA-H00029127-M01-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48 h, cells were immunostained for midbody markers (green), beta -tubulin (red), & DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29743530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00029127-m01_mouse-monoclonal-mgcracgap-racgap1-antibody-1g6-31020241535656.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Western-Blot-H00000174-M01-img0018.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunofluorescence of monoclonal antibody to alpha-Fetoprotein/AFP on HepG2 cell . [antibody concentration 30 ug/ml]](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000174-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunoperoxidase of monoclonal antibody to AFP ( Cat # H00000174-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunohistochemistry-Paraffin-H00000174-M01-img0009.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - AFP monoclonal antibody (M01), clone 1G7 Analysis of AFP expression in HepG2.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Western-Blot-H00000174-M01-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of monoclonal antibody to AFP on HepG2 cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000174-M01-img0014.jpg)
![Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AFP MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunoprecipitation-H00000174-M01-img0015.jpg)
![ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-ELISA-H00000174-M01-img0016.jpg)