| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56047SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44979 | |||
| Azide and BSA Free | NBP2-34434-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50032SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47910-0.1mg | |||
| IHC-Prediluted | NBP2-48332 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1013-0.02mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81803-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43784 | |||
| BSA Free | NBP2-34490-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24559-25ul | |||
| Azide and BSA Free | NBP3-43869 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28406 | |||
| FITC | NBP1-28405 | |||
| PE | NBP1-28407 | |||
![Western Blot: Sars Spike Protein Antibody [NB100-56047] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Sars-Spike-Protein-Antibody-Western-Blot-NB100-56047-img0003.jpg "Western Blot: Sars Spike Protein Antibody [NB100-56047] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 5 ug/ml.")
![Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Western-Blot-NBP2-34387-img0003.jpg "Western Blot: p53 Antibody (SPM590) [NBP2-34387] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (SPM590).")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM590) [NBP2-34387] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with P53 Monoclonal antibody (SPM590)](http://images.novusbio.com/fullsize/p53-Antibody-SPM590-Immunohistochemistry-Paraffin-NBP2-34387-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Mixed neuron and glia cultures stained with NBP2-50032 (red) and chicken antibody to Vimentin (green) and DNA (blue). NBP2-50032 reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while Vimentin was visualized in astrocytes and fibroblasts.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of adult rat hippocampus section stained with mouse mAb to MAP2A/B/C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to FOX2, dilution 1:2,000, in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. NBP2-50032 antibody labels all isotypes of the MAP2 protein which are found in the perikarya and dendrites of neurons. The FOX2 antibody stains the nuclei of a subset of neuronal cells.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Immunocytochemistry-Immunofluorescence-NBP2-50032-img0006.jpg)
![Western Blot: MAP2 Antibody (2C4) [NBP2-50032] - Analysis of tissue and cell lysates using mouse mAb to MAP2C/D, NBP2-50032, dilution 1:5,000 in green, and chicken pAb to Vimentin, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH/3T3 cell lysates. A band at about 280 kDa corresponds to full length intact MAP2A/2B isotypes. The bands that appear at about 70kDa represent MAP2C/D isotypes. Multiple bands in between are likely to be in vivo fragments of MAP2A/B. Only the SH-SY5Y cells, which have neuronal properties express MAP2 protein. Vimentin antibody binds to vimentin protein showing a single band at ~50 kDa, which is present in all preparations.](http://images.novusbio.com/fullsize/MAP2-Antibody-2C4-Western-Blot-NBP2-50032-img0005.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0026.jpg "Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human ovarian carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0023.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug K562 whole cell lysate/extract B. 30 ug THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0019.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug C8D30 whole cell lysate/extract. B. 30 ug BCL-1 whole cell lysate/extract. C. 30 ug Raw264.7 whole cell lysate/extract. 10 % SDS-PAGE. Dilution: 1:1000.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0020.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract.10 % SDS-PAGE. Dilution: 1:1000](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP1-33748-img0021.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - Paraffin-embedded human breast carcinoma. Bmi1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33748-img0022.jpg)
![Immunoprecipitation: BMI-1 Antibody [NBP1-33748] - Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of BMI-1 protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of BMI-1 protein by 2.5 ug of BMI-1antibody 10% SDS-PAGE The immunoprecipitated BMI-1protein was detected by BMI-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunoprecipitation-NBP1-33748-img0018.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-9102024209145.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog lung.BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420115062.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202419593519.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420131285.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242021927.jpg)
![Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody [NBP1-33748] - BMI-1 antibody detects BMI-1 protein at nucleus by immunofluorescent analysis.Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BMI-1 stained by BMI-1 antibody (NBP1-33748) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-910202420134048.jpg)
![Western Blot: BMI-1 Antibody [NBP1-33748] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BMI-1 antibody (NBP1-33748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-91020242063358.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: BMI-1 Antibody [NBP1-33748] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-1610202419414590.jpg)
![BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (NBP1-33748) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33748_rabbit-polyclonal-bmi-1-antibody-immunocytochemistry-immunofluorescence-261120251844101.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Placenta stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0002.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/763) [NBP2-44568] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/763)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunohistochemistry-Paraffin-NBP2-44568-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - This image was submitted via customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-763-Immunofluorescence-NBP2-44568-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (HPCA1/763) [NBP2-44568] - Immunofluorescence staining of retained human SVF adjacent to the site of cell-seeded scaffold transplantation. (A) Human SVF are visualized using human specific nuclear matrix antigen (NuMA). Immunofluorescence imaging of human-specific antibodies targeting CD34 (B) & CD31 (C). Scale bar: 50 μm (A), 100 μm (B,C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32766213), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-44568_mouse-monoclonal-cd34-antibody-hpca1-763-31020241535640.jpg)
![Western Blot: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-18H5-Western-Blot-NB600-1013-img0002.jpg "Western Blot: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis using our monoclonal and polyclonal antibodies. Both antibodies recognize a protein of about 36 kDa in total lysate from LECs but not from HUVEC.")
![Immunocytochemistry/Immunofluorescence: Podoplanin Antibody (18H5) [NB600-1013] - staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Podoplanin (0,5ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 was used 1:400.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-18H5-Immunocytochemistry-Immunofluorescence-NB600-1013-img0006.jpg)
![Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - analysis with human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-mouse IgG-PE was used.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-18H5-Flow-Cytometry-NB600-1013-img0005.jpg)
![Flow Cytometry: Podoplanin Antibody (18H5) [NB600-1013] - Fig. 1: Flow analysis of Podoplanin expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). As expected there is no expression of Podoplanin detectable in the HUVECs but a strong one in the HDMVEC.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-18H5-Flow-Cytometry-NB600-1013-img0001.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/Mouse-alpha-Smooth-Muscle-Actin-ELISA-Kit-Colorimetric-ELISA-NBP2-66429-img0002.jpg "ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.")
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - DRC image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-166202313361..jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-elisa-137202316216.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Samples were spiked with high concentrations of Mouse alpha-Smooth Muscle Actin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-132202416225244.jpg)
![ELISA: Mouse alpha-Smooth Muscle Actin ELISA Kit (Colorimetric) [NBP2-66429] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66429_mouse-alpha-smooth-muscle-actin-elisa-kit-colorimetric-4320249133249.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81803-img0026.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81803-img0012.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0025.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0021.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0022.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0023.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81803-img0024.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human tonsil and skeletal muscle tissues using [NBP2-34490]. Corresponding ITGAM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Immunohistochemistry-Paraffin-NBP2-34490-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human tonsil and skeletal muscle tissues using [NBP2-34490]. Corresponding ITGAM RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD11b Antibody (CL1719) [NBP2-34490] - Western Blot analysis using CD11b Antibody (CL1719) [NBP2-34490]. Lane 1: Marker [kDa] Lane 2: Human tonsil. Detection at ~190 kDa.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Western-Blot-NBP2-34490-img0008.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human cerebral cortex using CD11b Antibody (CL1719) [NBP2-34490] shows moderate membranous positivity in microglia.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Immunohistochemistry-Paraffin-NBP2-34490-img0018.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining in paraffin-embedded sections of human tonsil using CD11b Antibody (CL1719) [NBP2-34490] shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Immunohistochemistry-Paraffin-NBP2-34490-img0012.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining of human colon using CD11b Antibody (CL1719) [NBP2-34490] shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Immunohistochemistry-Paraffin-NBP2-34490-img0013.jpg)
![Immunohistochemistry-Paraffin: CD11b Antibody (CL1719) [NBP2-34490] - Immunohistochemical staining of Paraffin-embedded sections of human skeletal muscle using CD11b Antibody (CL1719) [NBP2-34490] shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/CD11b-Antibody-CL1719-Immunohistochemistry-Paraffin-NBP2-34490-img0014.jpg)
![Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of various cell lines, using beta-Actin antibody (NBP3-16886) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-4C4-Western-Blot-NBP3-16886-img0001.jpg "Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of various cell lines, using beta-Actin antibody (NBP3-16886) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of HeLa cells, using beta-Actin antibody (NBP3-16886) at 1:10000-1:320000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-4C4-Western-Blot-NBP3-16886-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Actin Antibody (4C4) [NBP3-16886] - Human liver cancer tissue using beta -Actin Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16886_rabbit-beta-actin-mab-4c4-immunohistochemistry-paraffin-11120249437..png)
![Western Blot: beta-Actin Antibody (4C4) [beta-Actin] - Western blot analysis of various lysates using beta-Actin Rabbit mAb at 1:50000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-16886_rabbit-beta-actin-mab-4c4-52202517311097.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Veins and venules in the interalveolar stroma of porcine mammary gland are positive for the human VCAM-1. Antigen retrieval by Proteinase K. AlexaFluor 488 anti-mouse antibody was used as a secondary detection antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Immunohistochemistry-Paraffin-NBP1-28404-img0005.jpg)
![Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - TNF alpha stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD106-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Flow-Cytometry-NBP1-28404-img0006.jpg)
![Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Analysis using the FITC conjugate of NBP1-28404. Staining of TNF alpha stimulated endothelial cell line HUV-EC-C.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Flow-Cytometry-NBP1-28404-img0003.jpg)
![Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of RPN2 decreased luminal B BC cell dissemination in vivo. Luminal B ER+ T47D cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting RPN2 (siRNA-RPN2) were injected + estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos with green fluorescent blood vessels & analyzed as described in materials & methods. (A) Migration in vitro (n = 6). (B)In vivo dissemination of transfected T47D in presence of E2 ± Neu (n = 23–26). Scale bar = 100 µm. (n = 23–26). (C) Western blot analysis for confirmation of siRNA-RPN2 transfection & VCAM-1, ICAM-1, & MUC-1 expression. (D) Focal adhesions area (n = 5–6). Scale bar = 10 µm. (E) Proliferation (n = 6). Representative images of zebrafish embryos with disseminated luminal B T47D BC cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated T47D & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, ***P < 0.001, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28404_mouse-monoclonal-vcam-1-cd106-antibody-1-g11b1-310202416154617.jpg)
![Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of U2AF1 decreased luminal A BC cell dissemination in vivo. Luminal A ER+ MCF-7 cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting U2AF1 (siRNA-U2AF1) were injected in presence of estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos, with green fluorescent blood vessels, & analyzed as described in materials & methods. (A) Migration in vitro (n = 6–12). (B)In vivo dissemination in presence of E2 ± Neu (n = 38–41). Scale bar = 100 µm. (C) Western blot analysis for confirmation of siRNA-U2AF1 transfection & ICAM-1, VCAM-1, & MUC-1 expression. (D) Focal adhesion area (n = 7). Scale bar = 10 µm. (E) Proliferation in vitro (n = 12). Representative images of zebrafish embryos with disseminated MCF-7 cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated MCF-7 cells & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, **P < 0.01, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28404_mouse-monoclonal-vcam-1-cd106-antibody-1-g11b1-31020241617356.jpg)