Sars Spike Protein Antibody

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Western Blot: Sars Spike Protein Antibody [NB100-56047] - Analysis of SARS Spike in (A) transfected mouse melanoma cell lysate and (B) untransfected cell lysate using this antibody. 5 ug/ml.

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Sars Spike Protein Antibody Summary

Immunogen
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 19-35 (CTTFDDVQAPNYTQHTS) from the S (Spike glycoprotein)(Spike Protein 1) for the Human SARS coronavirus (Genbank accession no. NP_828851.1)
Specificity
The was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • ELISA 0.5-2 ug/ml
Publications
Read Publications using NB100-56047.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Sars Spike Protein Antibody

  • E2 glycoprotein
  • E2
  • Human coronavirus spike glycoprotein
  • Peplomer protein
  • S glycoprotein
  • S
  • SARS coronavirus S protein
  • SARS coronavirus Spike Protein
  • SARS CoV S glycoprotein
  • SARS CoV Spike protein
  • SARS CoV
  • SARS-CoV S protein
  • SARS-COV Spike
  • SARSCoV
  • Severe acute respiratory syndrome spike glycoprotein
  • Severe acute respiratory syndrome virus spike glycoprotein
  • Spike glycoprotein
  • VGL2

Background

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Sars Spike Protein Antibody (NB100-56047)(10)

We have publications tested in 1 application: WB.


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WB
(5)
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Showing Publications 1 - 10 of 10.
Publications using NB100-56047 Applications Species
Narayanan K, Ramirez Si, Lokugamage Kg et al. Coronavirus nonstructural protein 1: Common and distinct functions in the regulation of host and viral gene expression Virus Res. 2014 Nov 26 [PMID: 25432065] (WB)

Details:
Sars Spike Protein antibody used for WB on Vero E6 cells which were either mock-infected (Mock) or infected with wt SARS-CoV (WT) or SARS-CoV-mt (mt), carrying the mutations K164A and H165A in nsp1, at an MOI of 5.
WB
Shulla A, Heald-Sargent T, Subramanya G et al. A transmembrane serine protease is linked to the severe acute respiratory syndrome coronavirus receptor and activates virus entry. J Virol. 2011 Jan [PMID: 21068237] (WB)

Details:
WB: SARS-Nucleocapsid protein (IMG-549) & SARS-Spike protein (IMG-541). 293T cells harvested 24 hours post infection with the SARS Coronavirus, Fig 9B.
WB
Lokugamage Kumari G, Yoshikawa-Iwata Naoko, Ito Naoto et al. Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV. Vaccine. 2008 Feb 6 [PMID: 18191004] (WB) WB
Huang C, Peters CJ, Makino S. Severe acute respiratory syndrome coronavirus accessory protein 6 is a virion-associated protein and is released from 6 protein-expressing cells. J Virol. 2007 May [PMID: 17344286] (WB)

Details:
SARS-Spike Protein (IMG-541) [WB, Fig1 (viral model)].
WB
Huang C, Ito N, Tseng CT, Makino S. Severe acute respiratory syndrome coronavirus 7a accessory protein is a viral structural protein. J Virol. 2006 Aug [PMID: 16840309]
Huang C, Narayanan K, Ito N et al. Severe acute respiratory syndrome coronavirus 3a protein is released in membranous structures from 3a protein-expressing cells and infected cells. J Virol. 2006 Jan [PMID: 16352545]
Bisht H, Roberts A, Vogel L et al. Neutralizing antibody and protective immunity to SARS coronavirus infection of mice induced by a soluble recombinant polypeptide containing an N-terminal segment of the spike glycoprotein. Virology. 2005 Apr 10 [PMID: 15780866]
Yang ZY, Werner HC, Kong WP et al. Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses. Proc Natl Acad Sci U S A. 2005 Jan 18 [PMID: 15642942]
Ho Y, Lin PH, Liu CY et al. Assembly of human severe acute respiratory syndrome coronavirus-like particles. Biochem Biophys Res Commun. 2004 Jun 11 [PMID: 15147946]
Bisht Himani, Roberts Anjeanette, Vogel Leatrice et al. Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice. Proc Natl Acad Sci U S A. 2004 Apr 27 [PMID: 15096611] (WB) WB

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Product General Protocols

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Video Protocols

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FAQs for Sars Spike Protein Antibody (NB100-56047). (Showing 1 - 3 of 3 FAQs).

  1. Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
    • NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
  2. I am looking for a primary antibody to sars spike protein to the new COVID-19.
    • NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
  3. Hi! does NB100-56047 cross-react with Sars-2?
    • The immunogen for NB100-56047 has no similarity to SARS-CoV-2. The immunogen for NB100-56562 one is 100% homologous.
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