| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45009 | |||
| Azide and BSA Free | NBP2-34577-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-1031SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93171-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95223-0.02ml | |||
![Western Blot: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Knockout-Validated-NBP2-32914-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Western blot shows lysates of HepG2 human liver carcinoma cells parental cell line and AFP knockout (KO) HepG2 cell line. PVDF membrane was probed with 1.0 ug/mL of Mouse Anti-Human AFP Monoclonal Antibody (Catalog # NBP2-32914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for AFP at approximately 74 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in the knockout HepG2 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - Formalin-fixed, paraffin-embedded human fetal liver stained with AFP MAb (C3).](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Immunohistochemistry-Paraffin-NBP2-32914-img0001.jpg)
![Flow (Intracellular): alpha-Fetoprotein/AFP Antibody (C3) [NBP2-32914] - An intracellular stain was performed on HepG2 cells with alpha-Fetoprotein/AFP Antibody [C3] NBP2-34577AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700. Image using the Alexa Fluor 700 Azide and BSA Free format of this antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-C3-Flow-Intracellular-NBP2-32914-img0010.jpg)
![Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/UNG-Antibody-Western-Blot-NB600-1031-img0003.jpg "Western Blot: UNG Antibody [NB600-1031] - Total protein from human HeLa cells, muse Neuro2A cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-UNG in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunohistochemistry-Paraffin: uracil-DNA glycosylase Antibody [NB600-1031] - Analysis of human testis using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/uracil-DNA-glycosylase-Antibody-Immunohistochemistry-Paraffin-NB600-1031-img0002.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0010.jpg "Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in Jurkat.")
![Immunohistochemistry-Paraffin: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Analysis of monoclonal antibody to RACGAP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Immunohistochemistry-Paraffin-H00029127-M01-img0007.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Western Blot analysis of RACGAP1 expression in K-562 ( Cat # L009V1 ).](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0003.jpg)
![Western Blot: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - RACGAP1 monoclonal antibody (M01), clone 1G6. Analysis of RACGAP1 expression in 293.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-Western-Blot-H00029127-M01-img0009.jpg)
![ELISA: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - Detection limit for recombinant GST tagged RACGAP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MgcRacGAP-RACGAP1-Antibody-1G6-ELISA-H00029127-M01-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: MgcRacGAP/RACGAP1 Antibody (1G6) [H00029127-M01] - iASPP-dependent localization of midbody markers.a Localization of midbody markers in control, iASPP-depleted, or CEP55-depleted HeLa cells. HeLa cells were transfected with indicated siRNAs. After 48 h, cells were immunostained for midbody markers (green), beta -tubulin (red), & DNA (blue). b Quantification of proportions of cells from a displaying defects in localization of markers at the midbody Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29743530), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00029127-m01_mouse-monoclonal-mgcracgap-racgap1-antibody-1g6-31020241535656.jpg)
![Immunocytochemistry/Immunofluorescence: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/GART-Antibody-4D6-1D5-Immunocytochemistry-Immunofluorescence-H00002618-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. Antibody concentration 2 ug/ml.](http://images.novusbio.com/fullsize/GART-Antibody-4D6-1D5-Immunohistochemistry-Paraffin-H00002618-M01-img0008.jpg)
![Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - GART monoclonal antibody (M01), clone 4D6-1D5 Analysis of GART expression in Hela S3 NE.](http://images.novusbio.com/fullsize/GART-Antibody-4D6-1D5-Western-Blot-H00002618-M01-img0010.jpg)
![Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART expression in transfected 293T cell line by GART monoclonal antibody (M01), clone 4D6-1D5.Lane 1: GART transfected lysate(46 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GART-Antibody-4D6-1D5-Western-Blot-H00002618-M01-img0011.jpg)
![Immunoprecipitation: GART Antibody (4D6-1D5) [H00002618-M01] - Analysis of GART transfected lysate using anti-GART monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GART MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/GART-Antibody-4D6-1D5-Immunoprecipitation-H00002618-M01-img0009.jpg)
![Western Blot: GART Antibody (4D6-1D5) [H00002618-M01] - The role of HIF-1 in purinosome formation.a, quantifying the number of purinosome-containing cells in normoxia or hypoxia (24 h) in purine-rich medium & normoxia in purine-depleted medium (purine -ve), cells in hypoxia transfected with siRNA to HIF-1 alpha (+ siRNA), & cells in purine-rich medium supplemented with DFX. Data shown are n = 3, mean ± S.E., total number of cells counted are shown in parentheses. b, time course of purinosome formation in hypoxia shows the number of purinosome-containing cells steadily increases after 3 h in hypoxia. Re-oxygenation of the samples after hypoxic incubation for 10 h reverts the number purinosome-containing cells back to normoxic levels. Data shown is n = 3, mean ± S.E., total number of cells counted are shown in parentheses. c, time course of HIF-1 alpha stabilization in hypoxia shows maximum HIF-1 alpha protein expression levels at 3 h in hypoxia, after which the HIF-1 alpha expression decreases. The positions of molecular markers are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. d, the effect of hypoxia on the transcription of purine biosynthesis enzymes measured by qPCR. Vascular endothelial growth factor (VEGF) & HIF-1 alpha are controls. Data shown are n = 5, mean ± S.E. e, the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, & no significant increase in the purine enzymes was detected between normoxic (21% oxygen) & hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot; uncropped blots with overlaid markers are deposited in the raw data files. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00002618-m01_mouse-monoclonal-gart-antibody-4d6-1d5-310202415485120.jpg)
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0013.jpg "Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.")
![Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Immunocytochemistry-Immunofluorescence-H00010146-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Immunohistochemistry-Paraffin-H00010146-M01-img0010.jpg)
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi ( Cat # H00010146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody (M01), clone 2F3 (Cat # H00010146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0011.jpg)
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody (M01), clone 2F3.Lane 1: G3BP transfected lysate(52.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0014.jpg)
![ELISA: G3BP1 Antibody (2F3) [H00010146-M01] - Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-ELISA-H00010146-M01-img0015.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Western-Blot-H00000174-M01-img0018.jpg "Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody (M01), clone 1G7.Lane 1: AFP transfected lysate(69 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunofluorescence of monoclonal antibody to alpha-Fetoprotein/AFP on HepG2 cell . [antibody concentration 30 ug/ml]](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000174-M01-img0019.jpg)
![Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Immunoperoxidase of monoclonal antibody to AFP ( Cat # H00000174-M01 ) on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunohistochemistry-Paraffin-H00000174-M01-img0009.jpg)
![Western Blot: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - AFP monoclonal antibody (M01), clone 1G7 Analysis of AFP expression in HepG2.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Western-Blot-H00000174-M01-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of monoclonal antibody to AFP on HepG2 cell. Antibody concentration 30 ug/ml.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunocytochemistry-Immunofluorescence-H00000174-M01-img0014.jpg)
![Immunoprecipitation: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Analysis of AFP transfected lysate using anti-AFP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AFP MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-Immunoprecipitation-H00000174-M01-img0015.jpg)
![ELISA: alpha-Fetoprotein/AFP Antibody (1G7) [H00000174-M01] - Detection limit for recombinant GST tagged AFP is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/alpha-Fetoprotein-AFP-Antibody-1G7-ELISA-H00000174-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human Skeletal Muscle, 5.0ug/ml.](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-56641-img0006.jpg)
![Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Western-Blot-NBP1-56641-img0007.jpg)
![Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-56641] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Troponin-I-Type-1-slow-skeletal-Antibody-Immunohistochemistry-Paraffin-NBP1-56641-img0005.jpg)
![Immunohistochemistry-Paraffin: Somatostatin Antibody [NBP2-93171] - Human appendix using Somatostatin (SST) antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Somatostatin-Antibody-Immunohistochemistry-Paraffin-NBP2-93171-img0002.jpg "Immunohistochemistry-Paraffin: Somatostatin Antibody [NBP2-93171] - Human appendix using Somatostatin (SST) antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Immunohistochemistry-Paraffin: Somatostatin Antibody [NBP2-93171] - Mouse pancreas using Somatostatin (SST) antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Somatostatin-Antibody-Immunohistochemistry-Paraffin-NBP2-93171-img0003.jpg)
![Western Blot: Somatostatin Antibody [NBP2-93171] - analysis of Rat brain, using Somatostatin (SST) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp2-93171_rabbit-polyclonal-somatostatin-antibody-western-blot-1511202310124..jpg)
![Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody - Azide and BSA Free [NBP2-93171] - Immunofluorescence analysis of paraffin-embedded mouse pancreas using Somatostatin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93171_rabbit-polyclonal-somatostatin-antibody-42202514275227.jpg)
![Immunocytochemistry/ Immunofluorescence: Somatostatin Antibody - Azide and BSA Free [NBP2-93171] - Immunofluorescence analysis of paraffin-embedded rat pancreas using Somatostatin Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93171_rabbit-polyclonal-somatostatin-antibody-42202514275210.jpg)
![Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Analysis of extracts of various cell lines, using MYH1 antibody at 1:410 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10038 dilution.Lysates/proteins: 63ug per lane. Blocking buffer: 41% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Myosin-Heavy-Chain-1-Antibody-Western-Blot-NBP2-95223-img0007.jpg "Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Analysis of extracts of various cell lines, using MYH1 antibody at 1:410 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10038 dilution.Lysates/proteins: 63ug per lane. Blocking buffer: 41% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] -analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time:3s.](http://images.novusbio.com/fullsize/nbp2-95223_rabbit-polyclonal-myosin-heavy-chain-1-antibody-western-blot-106202481447..jpg)
![Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Human skeletal muscle using MYH1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-95223_rabbit-polyclonal-myosin-heavy-chain-1-antibody-immunohistochemistry-paraffin-106202481839..jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Heavy Chain 1 Antibody - Azide and BSA Free [Myosin Heavy Chain 1] - Immunofluorescence analysis of paraffin-embedded mouse heart using Myosin Heavy Chain 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95223_rabbit-polyclonal-myosin-heavy-chain-1-antibody-52202516321462.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Heavy Chain 1 Antibody - Azide and BSA Free [Myosin Heavy Chain 1] - Immunofluorescence analysis of paraffin-embedded Human heart using Myosin Heavy Chain 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95223_rabbit-polyclonal-myosin-heavy-chain-1-antibody-52202516334160.jpg)
![Immunocytochemistry/ Immunofluorescence: Myosin Heavy Chain 1 Antibody - Azide and BSA Free [Myosin Heavy Chain 1] - Immunofluorescence analysis of paraffin-embedded rat heart using Myosin Heavy Chain 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95223_rabbit-polyclonal-myosin-heavy-chain-1-antibody-5220251628026.jpg)
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0003.jpg "Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - ARPE-19 monolayer on glass substrate. Fixative 4% PFA Blocking Goat Serum Dilution 1:200. Incubation 1h @RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-NBP2-37597-img0008.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunohistochemistry-Paraffin-NBP2-37597-img0007.jpg)
![Flow Cytometry: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Flow cytometric analysis of HepG2 cells using OTX2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Flow-Cytometry-NBP2-37597-img0005.jpg)
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-Immunofluorescence-NBP2-37597-img0004.jpg)
![ELISA: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-ELISA-NBP2-37597-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD19 Antibody (JF100-06) [NBP2-67229] - Staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunocytochemistry-Immunofluorescence-NBP2-67229-img0002.jpg)
![Immunohistochemistry-Paraffin: CD19 Antibody (JF100-06) [NBP2-67229] - Analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD19-Antibody-JF100-06-Immunohistochemistry-Paraffin-NBP2-67229-img0003.jpg)
![Western Blot: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of CD19 on different lysates with Rabbit anti-CD19 antibody at 1/1,000 dilution.Lane 1: Raji cell lysateLane 2: Daudi cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 61 kDaObserved band size: 90 kDaExposure time: 2 minutes;8% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-western-blot-1382024114350..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Daudi cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-138202411462..jpg)
![Flow Cytometry: CD19 Antibody (JF100-06) [NBP2-67229] -Analysis of Raji cells labeling CD19.Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-67229_rabbit-monoclonal-cd19-antibody-jf100-06-flow-cytometry-1382024114721..jpg)