| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61845-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37270-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61893-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98810-50ul | |||
![Western Blot: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Analysis using ALK mAb against human ALK (AA: 1366-1468) recombinant protein. (Expected MW is 36.9 kDa)](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-7A11A4-Western-Blot-NBP2-61845-img0004.jpg "Western Blot: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Analysis using ALK mAb against human ALK (AA: 1366-1468) recombinant protein. (Expected MW is 36.9 kDa)")
![Immunocytochemistry/Immunofluorescence: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Analysis of Hela cells using ALK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-7A11A4-Immunocytochemistry-Immunofluorescence-NBP2-61845-img0003.jpg)
![Western Blot: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Analysis using ALK mAb against HEK293 (1) and ALK (AA: 1366-1468)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-7A11A4-Western-Blot-NBP2-61845-img0005.jpg)
![ELISA: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-7A11A4-ELISA-NBP2-61845-img0001.jpg)
![ELISA: ALK/CD246 Antibody (7A11A4) [NBP2-61845] - Analysis of Hela cells using ALK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ALK-CD246-Antibody-7A11A4-ELISA-NBP2-61845-img0002.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0004.jpg "Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mouse mAb against HepG2 cell lysate.")
![Immunohistochemistry: Tie-1 Antibody (8D12B10) [NBP2-37270] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TIE1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Immunohistochemistry-NBP2-37270-img0005.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against human TIE1 (AA: 385-607) recombinant protein. (Expected MW is 50.6 kDa)](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0002.jpg)
![Western Blot: Tie-1 Antibody (8D12B10) [NBP2-37270] - Western blot analysis using TIE1 mAb against HEK293 (1) and TIE1 (AA: 385-607)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-Western-Blot-NBP2-37270-img0003.jpg)
![ELISA: Tie-1 Antibody (8D12B10) [NBP2-37270] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Tie-1-Antibody-8D12B10-ELISA-NBP2-37270-img0001.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0004.jpg "Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against human SYK (AA: 217-356) recombinant protein. (Expected MW is 41.4 kDa)")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Immunocytochemistry-Immunofluorescence-NBP2-61893-img0003.jpg)
![Flow Cytometry: SYK Antibody (2G11E6) [NBP2-61893] - Analysis of Hela cells using SYK mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Flow-Cytometry-NBP2-61893-img0002.jpg)
![Western Blot: SYK Antibody (2G11E6) [NBP2-61893] - Analysis using SYK mAb against HEK293 (1) and SYK (AA: 217-356)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-Western-Blot-NBP2-61893-img0005.jpg)
![ELISA: SYK Antibody (2G11E6) [NBP2-61893] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/SYK-Antibody-2G11E6-ELISA-NBP2-61893-img0001.jpg)
![Western Blot: TrkB Antibody (OTI2E1) - Azide and BSA Free [NBP2-74624] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiNTRK2.](http://images.novusbio.com/fullsize/TrkB-Antibody-OTI2E1-Azide-and-BSA-Free-Western-Blot-NBP2-74624-img0001.jpg "Western Blot: TrkB Antibody (OTI2E1) - Azide and BSA Free [NBP2-74624] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY NTRK2 (RC205129, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiNTRK2.")
![Immunocytochemistry/Immunofluorescence: TrkB Antibody (OTI2E1) - Azide and BSA Free [NBP2-74624] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TrkB.](http://images.novusbio.com/fullsize/TrkB-Antibody-OTI2E1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74624-img0002.jpg)
![Immunohistochemistry: TrkB Antibody (OTI2E1) - Azide and BSA Free [NBP2-74624] - Staining of paraffin-embedded Human lung tissue using anti-TrkB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/TrkB-Antibody-OTI2E1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74624-img0003.jpg)
![Western Blot: PRPK Antibody (OTI5B2) - Azide and BSA Free [NBP2-73675] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPK.](http://images.novusbio.com/fullsize/PRPK-Antibody-OTI5B2-Azide-and-BSA-Free-Western-Blot-NBP2-73675-img0001.jpg "Western Blot: PRPK Antibody (OTI5B2) - Azide and BSA Free [NBP2-73675] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPK.")
![Immunohistochemistry: PRPK Antibody (OTI5B2) - Azide and BSA Free [NBP2-73675] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PRPK-Antibody-OTI5B2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73675-img0003.jpg)
![Immunohistochemistry: PRPK Antibody (OTI5B2) - Azide and BSA Free [NBP2-73675] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PRPK-Antibody-OTI5B2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73675-img0002.jpg)
![Immunohistochemistry: PRPK Antibody (OTI5B2) - Azide and BSA Free [NBP2-73675] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PRPK-Antibody-OTI5B2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73675-img0004.jpg)
![Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-288202412461668.jpg "Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-298202414262644.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-29202410334061.jpg)
![Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Western-Blot-NBP2-67207-img0001.jpg "Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0004.jpg)
![Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Flow cytometric analysis of CaM Kinase II alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Flow-Cytometry-NBP2-67207-img0002.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaM Kinase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0003.jpg)
![Western Blot: p38 beta/MAPK11 Antibody [NBP2-98810] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Western-Blot-NBP2-98810-img0004.jpg "Western Blot: p38 beta/MAPK11 Antibody [NBP2-98810] - Lane A: K562 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:36 kDa")
![Immunohistochemistry-Paraffin: p38 beta/MAPK11 Antibody [NBP2-98810] - Immunochemical staining of human p38 beta/MAPK11 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/p38-beta-MAPK11-Antibody-Immunohistochemistry-Paraffin-NBP2-98810-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: p38 beta/MAPK11 Antibody [NBP2-98810] - Staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-98810 antibody (dilution ratio: 1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98810_rabbit-polyclonal-p38-beta-mapk11-antibody-235202521451726.png)
![Immunohistochemistry-Paraffin: p38 beta/p38 beta/MAPK11 Antibody [NBP2-98810] - Immunochemical staining of human p38 beta/MAPK11 in human liver with NBP2-98810 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-98810_rabbit-polyclonal-p38-beta-mapk11-antibody-23520251129357.png)
![Western Blot: KSR1 Antibody (JE57-75) [NBP3-32512] - Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32512) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-27820241631620.jpg "Western Blot: KSR1 Antibody (JE57-75) [NBP3-32512] - Western blot analysis of KSR1 on different lysates with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: HT-29 cell lysate Lane 4: HDLM-2 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 120/100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32512) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: KSR1 Antibody (JE57-75) [NBP3-32512] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue with Rabbit anti-KSR1 antibody (NBP3-32512) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32512) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-29820241435283.jpg)
![Flow Cytometry: KSR1 Antibody (JE57-75) [NBP3-32512] - Flow cytometric analysis of C6 cells labeling KSR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32512, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32512_rabbit-ksr1-mab-je57-75-29202416372119.jpg)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: ACTR2 Antibody (JE40-62) [NBP3-32048] - Western blot analysis of ACTR2 on different lysates with Rabbit anti-ACTR2 antibody (NBP3-32048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32048) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32048_rabbit-actr2-mab-je40-62-278202416285242.jpg "Western Blot: ACTR2 Antibody (JE40-62) [NBP3-32048] - Western blot analysis of ACTR2 on different lysates with Rabbit anti-ACTR2 antibody (NBP3-32048) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32048) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ACTR2 Antibody (JE40-62) [NBP3-32048] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-ACTR2 antibody (NBP3-32048) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32048) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32048_rabbit-actr2-mab-je40-62-298202414161881.jpg)
![Immunocytochemistry/ Immunofluorescence: ACTR2 Antibody (JE40-62) [NBP3-32048] - Immunocytochemistry analysis of SH-SY5Y cells labeling ACTR2 with Rabbit anti-ACTR2 antibody (NBP3-32048) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ACTR2 antibody (NBP3-32048) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32048_rabbit-actr2-mab-je40-62-2920241192328.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)