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![Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Western-Blot-NB500-141-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.](http://images.novusbio.com/fullsize/ERK1-[p-Thr202--p-Tyr204]-Antibody-Immunohistochemistry-NB500-141-img0004.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0006.jpg "Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with kidney at 2 ug/ml.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0002.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Analysis of anti-TNIK antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0003.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Prostate, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0004.jpg)
![Immunohistochemistry-Paraffin: TNIK Antibody [NLS7401] - Anti-TNIK antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/TNIK-Antibody-Immunohistochemistry-Paraffin-NLS7401-img0005.jpg)
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-168202375610..jpg "Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of paraffin-embedded human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.")
![Immunohistochemistry-Paraffin-NBP3-21480-AKT1 [p Ser473] Antibody (0113)- Human breast cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-AKT (Ser473) Antibody, Rabbit Monoclonal at 1:200 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-immunohistochemistry-paraffin-16820237586..jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3 treated with PDGFA 5 ug/mL, 5 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-168202375944.jpg)
![<b>Biological Strategies Validation. </b>Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved A431, untreated (-); treated with EGF (200 ng/mL, 15 min; +), using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution (upper) or anti-Akt antibody (lower).](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238120.jpg)
![Western Blot-NBP3-21480-AKT1 [p Ser473] Antibody (0113)-Analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5 ug/mL, 5 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-AKT (Ser473) rabbit monoclonal Antibody at 1:2000 dilution.](http://images.novusbio.com/fullsize/antibody/nbp3-21480_rabbit-akt1-p-ser-473-mab-0113-western-blot-16820238442..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3 treated with PDGFA (5 ug/mL, 5 min; +), using NBP3-21480 at 1:1000, 1:50000, 1:200000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-136202422345072.png)
![Western Blot: AKT1 [p Ser473] Antibody (0113) [NBP3-21480] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with PDGFA (5000 ng/mL, 5min; +) (line B); treated with PDGFA and -phosphatase (line C) using NBP3-21480 at 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp3-21480_rabbit-akt1-p-ser473-mab-0113-23520251628115.png)
![Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.](http://images.novusbio.com/fullsize/Sin1-MAPKAP1-Antibody-Western-Blot-NB100-56482-img0003.jpg "Western Blot: Sin1/MAPKAP1 Antibody [NB100-56482] - Analysis of Mip1 in human heart lysate in the 1) absence and 2) presence of immunizing peptide and in human skeletal muscle lysate in the 3) absence and 4) presence of immunizing peptide using this antibody. 5 ug/ml.")
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0007.jpg "Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Polycystin 2 Antibody [NBP1-30117] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Polycystin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-30117-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Polycystin 2 Antibody [NBP1-30117] - Detection of human Polycystin-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Polycystin-2 antibody NBP1-30117 used for IP at 3 ug per reaction. Polycystin-2 was also immunoprecipitated by another rabbit anti-Polycystin-2 antibody. For blotting immunoprecipitated Polycystin-2, NBP1-30117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Immunoprecipitation-NBP1-30117-img0005.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0007.jpg "Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Immunohistochemistry-Paraffin-NBP2-15553-img0003.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/BCKDK-Antibody-Western-Blot-NBP2-15553-img0002.jpg)
![Western Blot: BCKDK Antibody [NBP2-15553] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (NBP2-15553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15553_rabbit-polyclonal-bckdk-antibody-910202420104442.jpg)
![Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Western-Blot-NB100-97852-img0006.jpg "Western Blot: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of Phospho Human Histone H3 (pS10) by Western Blot. A549, HeLa, K562, and Jurkat cell lines were stimulated with 0.5 ug/ml of Calyculin A at 37 degrees C, 5% CO2 for 15 minutes. Cells were subsequently lysed and 1 ug of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody NB100-97852 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 ug/ml; Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: HIST1H3A [p Ser10] Antibody [NB100-97852] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:2,500.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-97852-img0004.jpg)
![Immunohistochemistry-Paraffin: HIST1H3A [p Ser10] Antibody [NB100-97852] - Section of human colon carcinoma (left) and mouse squamous cell carcinoma(right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) used at a dilution of 1:2,500 (0.08 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB100-97852-img0003.jpg)
![Flow Cytometry: HIST1H3A [p Ser10] Antibody [NB100-97852] - Detection of phospho Histone H3 Ser10. Asynchronous Jurkat cells were fixed in 1.5% PFA and permeabilized in 90% methanol. 1 million cells were stained with 0.4 ug anti-phospho Histone H3 (S10) NB100-97852 in a 150 ul reaction.](http://images.novusbio.com/fullsize/HIST1H3A-[p-Ser10]-Antibody-Flow-Cytometry-NB100-97852-img0002.jpg)
![Western Blot: ABL2 Antibody [NBP1-18875] - Whole cell lysate from HeLa cells. ARG was also immunoprecipitated by rabbit anti-ARG antibodies NBP1-18876 and NBP1-18877.](http://images.novusbio.com/fullsize/ABL2-Antibody-Western-Blot-NBP1-18875-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ABL2 Antibody [NBP1-18875] - Whole cell lysate from HeLa cells. ARG was also immunoprecipitated by rabbit anti-ARG antibodies NBP1-18876 and NBP1-18877.")
![Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0010.jpg "Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Yes Antibody [NBP1-31297] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Yes-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31297-img0006.jpg)
![Western Blot: Yes Antibody [NBP1-31297] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0008.jpg)
![Western Blot: Yes Antibody [NBP1-31297] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with c-Yes antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Yes-Antibody-Western-Blot-NBP1-31297-img0009.jpg)
![Western Blot: MAP4K5 Antibody [NBP1-31234] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, diluted at 1:5000.](http://images.novusbio.com/fullsize/MAP4K5-Antibody-Western-Blot-NBP1-31234-img0005.jpg "Western Blot: MAP4K5 Antibody [NBP1-31234] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: MAP4K5 Antibody [NBP1-31234] - Analysis of A431, using MAP4K5 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MAP4K5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31234-img0003.jpg)
![Immunohistochemistry-Paraffin: MAP4K5 Antibody [NBP1-31234] - SAS xenograft, using KHS antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MAP4K5-Antibody-Immunohistochemistry-Paraffin-NBP1-31234-img0004.jpg)
![Immunoprecipitation: MAP4K5 Antibody [NBP1-31234] - Immunoprecipitation of KHS protein from 293T whole cell extracts using 5 ug of KHS antibody. Western blot analysis was performed using KHS antibody.EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAP4K5-Antibody-Immunoprecipitation-NBP1-31234-img0006.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other Casein Kinase 1 alpha isotypes.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-Western-Blot-NBP2-50030-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody [NBP2-50030] - Analysis of whole rat spinal cord homogenate stained with NBP2-50030, at dilution of 1:10,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~38kDa and a less prominent band at ~34kDa corresponds to other Casein Kinase 1 alpha isotypes.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody [NBP2-50030] - HeLa cells stained with NBP2-50030 (red) and panspecific mouse monoclonal antibody to nuclear pore complexes NBP1-05390 (green). Casein Kinase 1 alpha has a particulate distribution both in the nucleus and the cytoplasm. Blue is the Hoechst DNA stain.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50030-img0003.jpg)
![Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27370-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27370-img0002.jpg)