| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93829-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35840-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02952-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35369-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37289-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37312-0.025ml | |||
![Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PDK4-Antibody-Western-Blot-NBP2-93829-img0007.jpg "Western Blot: PDK4 Antibody [NBP2-93829] - Analysis of various lysates, using PDK4 antibody at 1:2350 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Mouse heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0006.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Rat heart using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0004.jpg)
![Immunohistochemistry-Paraffin: PDK4 Antibody [NBP2-93829] - Human placenta using PDK4 Rabbit pAb (NBP2-93829) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PDK4-Antibody-Immunohistochemistry-Paraffin-NBP2-93829-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: PDK4 Antibody - Azide and BSA Free [PDK4] - Immunofluorescence analysis of U2OS cells using PDK4 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93829_rabbit-polyclonal-pdk4-antibody-5220251638091.jpg)
![Immunohistochemistry: MNK1 Antibody [NBP3-35840] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MNK1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35840_rabbit-mnk1-pab-91220241581294.jpg "Immunohistochemistry: MNK1 Antibody [NBP3-35840] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MNK1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: MNK1 Antibody [NBP3-35840] - Immunofluorescence analysis of NIH-3T3 cells using MNK1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35840_rabbit-mnk1-pab-912202415175246.jpg)
![Immunohistochemistry: MNK1 Antibody [NBP3-35840] - Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using MNK1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35840_rabbit-mnk1-pab-912202414154377.jpg)
![Western Blot: MNK1 Antibody [NBP3-35840] - Western blot analysis of various lysates, using MNK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35840_rabbit-mnk1-pab-912202414112815.jpg)
![Immunohistochemistry: MNK1 Antibody [NBP3-35840] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MNK1 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35840_rabbit-mnk1-pab-91220241410186.jpg)
![Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-10122024140443.jpg "Immunocytochemistry/ Immunofluorescence: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Confocal imaging of HeLa cells using ERK1/2 Rabbit mAb(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.")
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177017.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025177023.jpg)
![Western Blot: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Western Blot analysis of various lysates using ERK1/2 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-31120251744429.jpg)
![Immunohistochemistry: ERK1/2 Antibody (2Z8O1) [NBP3-35062] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ERK1/2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35062_rabbit-erk1-2-mab-2z8o1-3112025173186.jpg)
![Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2-Antibody-Western-Blot-NBP3-02952-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of HeLa cells, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: MEK2 Antibody [NBP3-02952] - Western blot analysis of extracts of various cell lines, using MEK2 antibody (NBP3-02952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/MEK2 Antibody-Western Blot-NBP3-02952-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519302962.jpg)
![Immunocytochemistry/ Immunofluorescence: MEK2 Antibody - Azide and BSA Free [NBP3-02952] - Immunofluorescence analysis of U2OS cells using [KO Validated] MEK2 Rabbit pAb (A14770) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02952_rabbit-polyclonal-mek2-antibody-52202519294585.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of C6 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411381284.jpg "Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of C6 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PKC beta Antibody [NBP3-35369] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411334057.jpg)
![Immunohistochemistry: PKC beta Antibody [NBP3-35369] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411433423.jpg)
![Immunohistochemistry: PKC beta Antibody [NBP3-35369] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-1012202411381212.jpg)
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-35369] - Immunofluorescence analysis of NIH-3T3 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35369_rabbit-pkc-beta-pab-3112025173159.jpg)
![Western Blot: Fyn Antibody (2A10) [NBP2-37588] - Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Western-Blot-NBP2-37588-img0002.jpg "Western Blot: Fyn Antibody (2A10) [NBP2-37588] - Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)")
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (2A10) [NBP2-37588] - Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Immunocytochemistry-Immunofluorescence-NBP2-37588-img0003.jpg)
![Immunohistochemistry: Fyn Antibody (2A10) [NBP2-37588] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Immunohistochemistry-NBP2-37588-img0005.jpg)
![Flow Cytometry: Fyn Antibody (2A10) [NBP2-37588] - Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Flow-Cytometry-NBP2-37588-img0004.jpg)
![ELISA: Fyn Antibody (2A10) [NBP2-37588] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-ELISA-NBP2-37588-img0001.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Western-Blot-NBP2-37289-img0003.jpg "Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of prostate cancer tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Immunohistochemistry-Paraffin-NBP2-37289-img0007.jpg)
![Flow Cytometry: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of HepG2 cells using CSF1R mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Flow-Cytometry-NBP2-37289-img0004.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against human CSF1R (AA: 344-497) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Western-Blot-NBP2-37289-img0002.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of cervical cancer tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Immunohistochemistry-Paraffin-NBP2-37289-img0008.jpg)
![ELISA: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-ELISA-NBP2-37289-img0001.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0004.jpg "Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.")
![Immunohistochemistry: p38 Antibody (10B11C8) [NBP2-37312] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Immunohistochemistry-NBP2-37312-img0005.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0002.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0003.jpg)
![ELISA: p38 Antibody (10B11C8) [NBP2-37312] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-ELISA-NBP2-37312-img0001.jpg)
![Immunocytochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain tissue, senile plaques caused by Alzheimer's.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunocytochemistry-NB100-59014-img0004.jpg "Immunocytochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain tissue, senile plaques caused by Alzheimer's.")
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0010.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0003.jpg)
![Immunohistochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of the brain, hypothalamus. Neurons in the adjacent basal nucleus of Meynert](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-NB100-59014-img0005.jpg)
![Immunohistochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain, substantia nigra.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-NB100-59014-img0006.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0009.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0008.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-278202415295566.jpg "Western Blot: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Western blot analysis of Casein Kinase 2 alpha on different lysates with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32228) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western Blot: RSK4 Antibody (PSH02-04) [NBP3-32920] - Western blot analysis of RSK4 on different lysates with Rabbit anti-RSK4 antibody (NBP3-32920) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Caco-2 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: MDA-MB-468 cell lysate Lane 5: Raji cell lysate Lane 6: Ramos cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32920) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32920_rabbit-rsk4-mab-psh02-04-288202412442926.jpg "Western Blot: RSK4 Antibody (PSH02-04) [NBP3-32920] - Western blot analysis of RSK4 on different lysates with Rabbit anti-RSK4 antibody (NBP3-32920) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Caco-2 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: MDA-MB-468 cell lysate Lane 5: Raji cell lysate Lane 6: Ramos cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32920) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RSK4 Antibody (PSH02-04) [NBP3-32920] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RSK4 antibody (NBP3-32920) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32920) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32920_rabbit-rsk4-mab-psh02-04-298202414333678.jpg)
![Immunocytochemistry/ Immunofluorescence: RSK4 Antibody (PSH02-04) [NBP3-32920] - Immunocytochemistry analysis of PC-12 cells labeling RSK4 with Rabbit anti-RSK4 antibody (NBP3-32920) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RSK4 antibody (NBP3-32920) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32920_rabbit-rsk4-mab-psh02-04-2920241023424.jpg)
![Flow Cytometry: RSK4 Antibody (PSH02-04) [NBP3-32920] - Flow cytometric analysis of Caco-2 cells labeling RSK4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32920, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32920_rabbit-rsk4-mab-psh02-04-392024884688.jpg)