| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85795-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97476-0.2mg | |||
| BSA Free | NBP1-97476-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74048SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18545-25ul | |||
| Azide and BSA Free | NBP3-44167 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18575-25ul | |||
| Azide and BSA Free | NBP3-44169 | |||
![Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85795] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85795-img0006.jpg "Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85795] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.")
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Analysis in human skeletal muscle and liver tissues. Corresponding MEF2D RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney, liver, placenta and skeletal muscle using Anti-MEF2D antibody NBP1-85795 (A) shows similar protein distribution across tissues to independent antibody NBP1-85788 (B).](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0015.jpg)
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0016.jpg)
![Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518373813.jpg "<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Rhodopsin antibody [HL2668] (NBP3-48717) detects Rhodopsin protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4C for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4C for 1 hour.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-flow-cytometry-1411202518365924.jpg)
![Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518324111.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518424110.jpg)
![Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-14112025184336.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518401615.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518415219.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-141120251840548.jpg)
![Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.](http://images.novusbio.com/fullsize/MEK1-[p-Thr292]-Antibody-Western-Blot-NB500-145-img0001.jpg "Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.")
![Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97476-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97476-img0001.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0006.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Staining Phospho-Smad3(S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunocytochemistry-Immunofluorescence-NBP2-67513-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0009.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Phospho-Smad3(S423/S425) on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0003.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Lane 1: K562 cells, whole cell lysate, 10ug/lane, Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-Smad3(S423/S425) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 48 kDa Observed band size: 55 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0004.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on different lysates with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0008.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0029.jpg "Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Immunohistochemistry-NB100-2339-img0028.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0003.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-42862-img0007.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000")
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0005.jpg)
![Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (NBP2-42862) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42862_rabbit-polyclonal-histone-h4-trimethyl-lys20-antibody-immunocytochemistry-immunofluorescence-26112025182906.jpg)
![Western Blot: LSP1 Antibody [NBP1-74048] - Western blot analysis of LSP1 in Ramos (1) and Daudi (2) cell lysates.](http://images.novusbio.com/fullsize/LSP1-Antibody-Western-Blot-NBP1-74048-img0003.jpg "Western Blot: LSP1 Antibody [NBP1-74048] - Western blot analysis of LSP1 in Ramos (1) and Daudi (2) cell lysates.")
![Immunohistochemistry-Paraffin: LSP1 Antibody [NBP1-74048] - IHC analysis of LSP1 in mouse colon tissue. Abundant membrane staining is visible in the epithelial cells.](http://images.novusbio.com/fullsize/LSP1-Antibody-Immunohistochemistry-Paraffin-NBP1-74048-img0002.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0016.jpg "Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in NIH/3T3.")
![Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of monoclonal antibody to STXBP1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Immunohistochemistry-Paraffin-H00006812-M01-img0012.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1 Analysis of STXBP1 expression in HeLa.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0014.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0015.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of STXBP1 expression in transfected 293T cell line by STXBP1 monoclonal antibody (M01), clone 6D1.Lane 1: STXBP1 transfected lysate (Predicted MW: 68.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0017.jpg)
![Sandwich ELISA: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Detection limit for recombinant GST tagged STXBP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Sandwich-ELISA-H00006812-M01-img0013.jpg)
![Western Blot: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Western-Blot-NBP3-18545-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of mouse brain shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0007.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cerebral cortex and liver tissues using NBP3-18545 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0001.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0002.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0003.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0004.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0006.jpg)
![Western Blot: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Western-Blot-NBP3-18575-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of mouse cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0007.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cerebral cortex and liver tissues using NBP3-18575 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0001.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0002.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0003.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human cerebellum shows moderate to strong positivity in granular layer cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0004.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0006.jpg)