| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74048SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18575-25ul | |||
| Azide and BSA Free | NBP3-44169 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18545-25ul | |||
| Azide and BSA Free | NBP3-44167 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18058SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72156 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73812 | |||
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser529] Antibody-Western Blot-NBP1-77808-img0005.jpg "Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG used to develop blot using a chemiluminescent detection method. Other detection methods will yield similar results.")
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - pS529 shows phospho p65 staining in carcinoma cells. western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS529 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0003.jpg)
![Western Blot: RelA/NFkB p65 [p Ser529] Antibody [NBP1-77808] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 (NBP1-77808) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529]-Antibody-Western-Blot-NBP1-77808-img0004.jpg)
![Western Blot: LSP1 Antibody [NBP1-74048] - Western blot analysis of LSP1 in Ramos (1) and Daudi (2) cell lysates.](http://images.novusbio.com/fullsize/LSP1-Antibody-Western-Blot-NBP1-74048-img0003.jpg "Western Blot: LSP1 Antibody [NBP1-74048] - Western blot analysis of LSP1 in Ramos (1) and Daudi (2) cell lysates.")
![Immunohistochemistry-Paraffin: LSP1 Antibody [NBP1-74048] - IHC analysis of LSP1 in mouse colon tissue. Abundant membrane staining is visible in the epithelial cells.](http://images.novusbio.com/fullsize/LSP1-Antibody-Immunohistochemistry-Paraffin-NBP1-74048-img0002.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0029.jpg "Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Immunohistochemistry-NB100-2339-img0028.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0003.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0016.jpg "Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in NIH/3T3.")
![Immunohistochemistry-Paraffin: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of monoclonal antibody to STXBP1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Immunohistochemistry-Paraffin-H00006812-M01-img0012.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1 Analysis of STXBP1 expression in HeLa.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0014.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - STXBP1 monoclonal antibody (M01), clone 6D1. Analysis of STXBP1 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0015.jpg)
![Western Blot: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Analysis of STXBP1 expression in transfected 293T cell line by STXBP1 monoclonal antibody (M01), clone 6D1.Lane 1: STXBP1 transfected lysate (Predicted MW: 68.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Western-Blot-H00006812-M01-img0017.jpg)
![Sandwich ELISA: Syntaxin-BP1 Antibody (6D1) [H00006812-M01] - Detection limit for recombinant GST tagged STXBP1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-BP1-Antibody-6D1-Sandwich-ELISA-H00006812-M01-img0013.jpg)
![Western Blot: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Western-Blot-NBP3-18575-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of mouse cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0007.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Analysis in human cerebral cortex and liver tissues using NBP3-18575 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0001.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0002.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0003.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human cerebellum shows moderate to strong positivity in granular layer cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0004.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human testis shows moderate to strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12836) [NBP3-18575] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12836-Immunohistochemistry-Paraffin-NBP3-18575-img0006.jpg)
![Western Blot: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Western-Blot-NBP3-18545-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cell line U-2 OS and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of mouse brain shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0007.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Analysis in human cerebral cortex and liver tissues using NBP3-18545 antibody. Corresponding CALB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0001.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0002.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0003.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0004.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0005.jpg)
![Immunohistochemistry-Paraffin: Calretinin Antibody (CL12842) [NBP3-18545] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calretinin-Antibody-CL12842-Immunohistochemistry-Paraffin-NBP3-18545-img0006.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:3000.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0001.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0003.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0004.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0005.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0006.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-91020242041236.jpg)
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-9102024205680.jpg)
![Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] - FAK antibody detects FAK protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.FAK stained by FAK antibody (NBP3-13071) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-910202420115066.jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025124758..jpg "<b>Genetic Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein by immunofluorescent analysis.Sample: DIV11 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: MAP2, a microtubule marker, stained by MAP2 antibody [HM-2] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunohistochemistry-3042025123354..jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Tau mAb (HL2736) [NBP3-45160] - Tau antibody [HL2736] detects Tau protein at cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Tau stained by Tau antibody [HL2736] diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-immunocytochemistry-immunofluorescence-3042025125627..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025125836..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513045..jpg)
![Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513345..jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-304202513540..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Rabbit Tau mAb (HL2736) [NBP3-45160] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-3042025132854..jpg)
![<b>Orthogonal Strategies Validation. </b>Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody [HL2736] (NBP3-45160) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.](http://images.novusbio.com/fullsize/nbp3-45160_rabbit-tau-mab-hl2736-azide-and-bsa-free-western-blot-141120251831211.jpg)
![Tau antibody [HL2736] detects Tau protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse eye.Green: Tau stained by Tau antibody [HL2736] (NBP3-45160) diluted at 1:250.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-45161_rabbit-tau-mab-hl2802-azide-and-bsa-free-immunohistochemistry-frozen-14112025185543.jpg)
![Immunocytochemistry/Immunofluorescence: Nephrin Antibody [NBP3-18058] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Nephrin Antibody (NBP3-18058) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18058-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nephrin Antibody [NBP3-18058] - RCC4 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Nephrin Antibody (NBP3-18058) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of mouse kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0002.jpg)
![Immunohistochemistry-Paraffin: Nephrin Antibody [NBP3-18058] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of Nephrin antibody (NBP3-18058). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Nephrin-Antibody-Immunohistochemistry-Paraffin-NBP3-18058-img0001.jpg)
![Western Blot: CDCP1 Antibody (2C1) [NBP2-03459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Western-Blot-NBP2-03459-img0002.jpg "Western Blot: CDCP1 Antibody (2C1) [NBP2-03459] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1.")
![Immunocytochemistry/Immunofluorescence: CDCP1 Antibody (2C1) [NBP2-03459] Staining of COS7 cells transiently transfected by pCMV6-ENTRY CDCP1.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Immunocytochemistry-Immunofluorescence-NBP2-03459-img0007.jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody (2C1) [NBP2-03459] - Staining of paraffin-embedded Human prostate tissue using anti-CDCP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-03459-img0006.jpg)
![Flow Cytometry: CDCP1 Antibody (2C1) [NBP2-03459] - Analysis of Jurkat cells, using anti-CDCP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Flow-Cytometry-NBP2-03459-img0001.jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody (2C1) [NBP2-03459] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDCP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-03459-img0003.jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody (2C1) [NBP2-03459] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDCP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-03459-img0004.jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody (2C1) [NBP2-03459] - Staining of paraffin-embedded Human lymphoma tissue using anti-CDCP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CDCP1-Antibody-2C1-Immunohistochemistry-Paraffin-NBP2-03459-img0005.jpg)
![Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Western-Blot-NB100-74357-img0002.jpg "Western Blot: epithelial Sodium Channel alpha Antibody [NB100-74357] - Analysis of 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: epithelial Sodium Channel alpha Antibody [NB100-74357] - performed using 70% confluent log phase HEK 293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(tm) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with alpha ENaC Rabbit Polyclonal Antibody at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(tm) Secondary Antibody, Alexa Fluor(R) 488 conjugate) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.](http://images.novusbio.com/fullsize/epithelial-Sodium-Channel-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74357-img0003.jpg)
![Western Blot: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRP3 .](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Western-Blot-NBP2-45528-img0008.jpg "Western Blot: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRP3 .")
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0007.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0001.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0002.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0003.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0004.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0005.jpg)
![Immunohistochemistry: RASGRP3 Antibody (2C11) [NBP2-45528] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/RASGRP3-Antibody-2C11-Immunohistochemistry-NBP2-45528-img0006.jpg)