DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." alt="CD5 was detected in immersion fixed paraffin-embedded sections of human mantle cell lymphoma using Mouse Anti-Human CD5 Monoclonal Antibody (Catalog # NBP3-21215) at 1:50 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21215-25ul | |||
| Azide and BSA Free | NBP3-44269 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84584-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88700-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90269-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37429-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96425-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
![Western Blot: ATP Citrate Lyase Antibody [NBP1-90267] - Analysis using Anti-ACLY antibody NBP1-90267 (A) shows similar pattern to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90267-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATP Citrate Lyase Antibody [NBP1-90267] - Analysis using Anti-ACLY antibody NBP1-90267 (A) shows similar pattern to independent antibody NBP1-90268 (B).")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0013.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows no positivity in tumor cells as expected.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0010.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human breast cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0011.jpg)
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody [NBP1-84584] - Staining of human kidney shows weak to moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-Immunohistochemistry-Paraffin-NBP1-84584-img0012.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88700 (A) shows similar protein distribution across tissues to independent antibody NBP1-88701 (B).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0009.jpg)
![Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Western-Blot-NBP1-88700-img0005.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lymph node shows moderate membranous positivity.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0011.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0012.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0013.jpg)
![ELISA: Mouse LBP ELISA Kit (Colorimetric) [NBP2-68052] - Samples were spiked with high concentrations of Mouse LBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68052_mouse-lbp-elisa-kit-colorimetric-132202416251798.jpg "ELISA: Mouse LBP ELISA Kit (Colorimetric) [NBP2-68052] - Samples were spiked with high concentrations of Mouse LBP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse LBP ELISA Kit (Colorimetric) [NBP2-68052] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68052_mouse-lbp-elisa-kit-colorimetric-432024932696.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90269 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney, pancreas, prostate and testis using Anti-ACLY antibody NBP1-90269 (A) shows similar protein distribution across tissues to independent antibody NBP1-90268 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0019.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0015.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody [NBP1-90269] - Staining of human prostate shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Immunohistochemistry-Paraffin-NBP1-90269-img0018.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ATP Citrate Lyase Antibody [NBP1-90269] - Analysis using Anti-ACLY antibody NBP1-90269 (A) shows similar pattern to independent antibody NBP1-90268 (B).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-Western-Blot-NBP1-90269-img0010.jpg)
![Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Western-Blot-NBP2-37429-img0003.jpg "Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mouse mAb against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Immunofluorescence analysis of HeLa cells using ACLY mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Immunocytochemistry-NBP2-37429-img0009.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded endometrial cancer tissues using ACLY mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Immunohistochemistry-Paraffin-NBP2-37429-img0011.jpg)
![Flow Cytometry: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Flow cytometric analysis of HeLa cells using ACLY mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Flow-Cytometry-NBP2-37429-img0005.jpg)
![Western Blot: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Western blot analysis using ACLY mAb against human ACLY recombinant protein. (Expected MW is 46.7 kDa)](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Western-Blot-NBP2-37429-img0002.jpg)
![Immunohistochemistry-Paraffin: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Analysis of paraffin-embedded esophageal cancer tissues using ACLY mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-Immunohistochemistry-Paraffin-NBP2-37429-img0010.jpg)
![ELISA: ATP Citrate Lyase Antibody (5F8D11) [NBP2-37429] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ATP-Citrate-Lyase-Antibody-5F8D11-ELISA-NBP2-37429-img0001.jpg)
![Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Western-Blot-NB110-96425-img0004.jpg "Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (NB110-96425). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96425) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-N27F3-4-Immunohistochemistry-NB110-96425-img0007.jpg)
![Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 ug per lane. Used at a dilution of 1:500 for overnight at 4C.](http://images.novusbio.com/fullsize/MARK2-[p-Thr595]-Antibody-Western-Blot-NBP1-78028-img0002.jpg "Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 ug per lane. Used at a dilution of 1:500 for overnight at 4C.")
![Western Blot: MARK2 [p Thr595] Antibody [NBP1-78028] - Lane 1: 293T cells untransfected. Lane 2: 293T cells transfected with MARK2 and PKC-zeta. Lane 3: 293T cells transfected with MARK2. Load: 35 ug per lane. Used at a dilution of 1:1000 for overnight at 4C.](http://images.novusbio.com/fullsize/MARK2-[p-Thr595]-Antibody-Western-Blot-NBP1-78028-img0001.jpg)
![Western Blot of Rabbit MARK2 [p Thr595] Antibody antibody. Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 ug per lane. Primary antibody: MARK2 pT595 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~83 kDa for MARK2pT595.](http://images.novusbio.com/fullsize/nbp1-78028_rabbit-polyclonal-mark2-p-thr595-antibody-255202313135151.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining in human thyroid gland and skeletal muscle tissues using NBP1-81802 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0022.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining in human thyroid gland and skeletal muscle tissues using NBP1-81802 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81802-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81802-img0011.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Western-Blot-NBP1-81802-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0015.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0020.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81802] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-Immunohistochemistry-Paraffin-NBP1-81802-img0021.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0007.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0002.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0006.jpg)
