| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21204-25ul | |||
| Azide and BSA Free | NBP3-44326 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44033 | |||
| BSA Free | NBP2-30458-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90327-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85795-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44034 | |||
| BSA Free | NBP2-52929-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88700-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97476-0.2mg | |||
| BSA Free | NBP1-97476-0.025mg | |||
![Western Blot: MEF2A Antibody (1C8) [H00004205-M15] - Analysis of MEF2A expression in MCF-7 (Cat # L046V1).](http://images.novusbio.com/fullsize/MEF2A-Antibody-1C8-Western-Blot-H00004205-M15-img0008.jpg "Western Blot: MEF2A Antibody (1C8) [H00004205-M15] - Analysis of MEF2A expression in MCF-7 (Cat # L046V1).")
![Immunocytochemistry/Immunofluorescence: MEF2A Antibody (1C8) [H00004205-M15] - Analysis of monoclonal antibody to MEF2A on MCF-7 cell. Image from verified customer review](http://images.novusbio.com/fullsize/MEF2A-Antibody-1C8-Immunocytochemistry-Immunofluorescence-H00004205-M15-img0009.jpg)
![Western Blot: MEF2A Antibody (1C8) [H00004205-M15] - Analysis of MEF2A expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/MEF2A-Antibody-1C8-Western-Blot-H00004205-M15-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2A Antibody (1C8) [H00004205-M15] - Analysis of monoclonal antibody to MEF2A on MCF-7 cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MEF2A-Antibody-1C8-Immunocytochemistry-Immunofluorescence-H00004205-M15-img0006.jpg)
![ELISA: MEF2A Antibody (1C8) [H00004205-M15] - Detection limit for recombinant GST tagged MEF2A is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MEF2A-Antibody-1C8-ELISA-H00004205-M15-img0004.jpg)
![Western Blot: LYRIC Antibody (CL0401) [NBP2-30458] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Western-Blot-NBP2-30458-img0008.jpg "Western Blot: LYRIC Antibody (CL0401) [NBP2-30458] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of U-251 cells using the Anti-MTDH monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunocytochemistry-Immunofluorescence-NBP2-30458-img0016.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human placenta shows absence of staining in the trophoblast layer (negative control).](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0015.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0009.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human testis shows strong cytoplasmic immunoreactivity in the seminiferous tubules.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0010.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human colon shows moderate cytoplasmic positivity in the glandular cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0011.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human colorectal cancer shows moderate cytoplasmic immunoreactivity in cancer cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0012.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human breast cancer (ductal carcinoma) shows strong cytoplasmic positivity in cancer cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0013.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0401) [NBP2-30458] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate cytoplasmic immunoreactivity in cancer cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0401-Immunohistochemistry-Paraffin-NBP2-30458-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: LYRIC Antibody [NBP1-90327] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/LYRIC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90327-img0007.jpg "Immunocytochemistry/Immunofluorescence: LYRIC Antibody [NBP1-90327] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: LYRIC Antibody [NBP1-90327] - Staining of human placenta shows moderate to strong cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-Immunohistochemistry-Paraffin-NBP1-90327-img0011.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody [NBP1-90327] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-Immunohistochemistry-Paraffin-NBP1-90327-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: LYRIC Antibody [NBP1-90327] - Analysis using Anti-MTDH antibody NBP1-90327 (A) shows similar pattern to independent antibody NBP1-90328 (B).](http://images.novusbio.com/fullsize/LYRIC-Antibody-Western-Blot-NBP1-90327-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85795] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85795-img0006.jpg "Immunocytochemistry/Immunofluorescence: MEF2D Antibody [NBP1-85795] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.")
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Analysis in human skeletal muscle and liver tissues. Corresponding MEF2D RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney, liver, placenta and skeletal muscle using Anti-MEF2D antibody NBP1-85795 (A) shows similar protein distribution across tissues to independent antibody NBP1-85788 (B).](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0015.jpg)
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-85795] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-85795-img0016.jpg)
![Western Blot: LYRIC Antibody (CL0397) [NBP2-52929] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Western-Blot-NBP2-52929-img0008.jpg "Western Blot: LYRIC Antibody (CL0397) [NBP2-52929] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of U-251 cells using the Anti-MTDH monoclonal antibody, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunocytochemistry-Immunofluorescence-NBP2-52929-img0009.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human testis shows strong cytoplasmic positivity in seminiferous tubules.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0007.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human breast cancer shows strong cytoplasmic immunoreactivity in cancer cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0001.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human colorectal cancer shows cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0002.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human liver cancer shows granular cytoplasmic immunoreactivity in tumor cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0003.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human placenta shows absence of staining in the placental trophoblast (negative control).](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0004.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human prostate cancer shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0005.jpg)
![Immunohistochemistry-Paraffin: LYRIC Antibody (CL0397) [NBP2-52929] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LYRIC-Antibody-CL0397-Immunohistochemistry-Paraffin-NBP2-52929-img0006.jpg)
![Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518373813.jpg "<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Rhodopsin antibody [HL2668] (NBP3-48717) detects Rhodopsin protein by flow cytometry analysis.Sample: Non-transfected and transfected 293T cells were fixed in 4% paraformaldehyde at 4C for 15 min and washed with 0.1% saponin buffer. The cells were following stained with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:50 or a Rabbit monoclonal IgG isotype control at 4C for 1 hour.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-flow-cytometry-1411202518365924.jpg)
![Human tissue extract was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518324111.jpg)
![<b>Orthogonal Strategies Validation. </b>Unboiled Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-1411202518424110.jpg)
![Rat tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-14112025184336.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518401615.jpg)
![Rhodopsin antibody [HL2668] detects Rhodopsin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Rhodopsin stained by Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-immunohistochemistry-paraffin-1411202518415219.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody [HL2668] (NBP3-48717) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48717_rabbit-rhodopsin-mab-hl2668-western-blot-141120251840548.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung and skeletal muscle tissues. Corresponding ICAM1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney, lung, lymph node and skeletal muscle using Anti-ICAM1 antibody NBP1-88700 (A) shows similar protein distribution across tissues to independent antibody NBP1-88701 (B).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0009.jpg)
![Western Blot: ICAM-1/CD54 Antibody [NBP1-88700] - Analysis in human lung tissue.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Western-Blot-NBP1-88700-img0005.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0014.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lymph node shows moderate membranous positivity.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0011.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0012.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody [NBP1-88700] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Immunohistochemistry-Paraffin-NBP1-88700-img0013.jpg)
![Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.](http://images.novusbio.com/fullsize/MEK1-[p-Thr292]-Antibody-Western-Blot-NB500-145-img0001.jpg "Western Blot: MEK1 [p Thr292] Antibody [NB500-145] - Recombinant wild type and mutant MEK 1 showing immunolabeling of the ~45k MEK-1 protein phosphorylated at Thr292. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T292A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.")
![Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97476-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97476-img0001.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0006.jpg "Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on A549 cell lysates. Lane 1 : A549 cells, whole cell lysate, 10 ug /lane. Lane 2 : A549 cells were treated with 5 ng/ml TGF beta1 for 24 hours, whole cell lysates, 10 ug/lane. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody Anti-Smad3 [p Ser423, p Ser425] (1/500) , Anti-Smad3 antibody (1/500) and Anti-GAPDH antibody (1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 3 seconds")
![Immunocytochemistry/Immunofluorescence: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Staining Phospho-Smad3(S423/S425) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunocytochemistry-Immunofluorescence-NBP2-67513-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0009.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Phospho-Smad3(S423/S425) on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0003.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Lane 1: K562 cells, whole cell lysate, 10ug/lane, Lane 2: K562 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane. All lanes : Anti-Phospho-Smad3(S423/S425) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 48 kDa Observed band size: 55 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 3 minutes 43 seconds](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0004.jpg)
![Western Blot: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of Smad3 [p Ser423, p Ser425] on different lysates with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Western-Blot-NBP2-67513-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue using anti-Phospho-Smad3(S423/S425) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded human large intestine tissue with Rabbit anti-Phospho-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0007.jpg)
![Immunohistochemistry-Paraffin: Smad3 [p Ser423, p Ser425] Antibody (ST0493) [NBP2-67513] - Analysis of paraffin-embedded mouse skin tissue with Rabbit anti-Smad3 [p Ser423, p Ser425] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Smad3-p-Ser423-p-Ser425-Antibody-ST0493-Immunohistochemistry-Paraffin-NBP2-67513-img0008.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0029.jpg "Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Detection of Human Phospho MCM2 (S53) by Western Blot. Samples: Whole cell lysate (5 and 15 ug for WB; 1 mg for IP, 20% of IP loaded) from asynchronous 293T cells. For IP/WB, MCM2 was immunoprecipitated using A300-191A, which recognizes total MCM2. The immunoprecipitate was mock treated (-) or treated (+) with phosphatase (PPase). Antibody: Affinity purified rabbit anti-phospho MCM2 (S53) antibody NB100-2339 used at 0.1 ug/ml. To examine total MCM2, the membrane was stripped and blotted with A300-191A at 0.1 ug/ml. For blotting immunoprecipitated MCM2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunohistochemistry: MCM2 [p Ser53] Antibody [NB100-2339] - Sample: FFPE sections of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 phospho MCM2 (S53) fs20 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Immunohistochemistry-NB100-2339-img0028.jpg)
![Western Blot: MCM2 [p Ser53] Antibody [NB100-2339] - Whole cell lysate (50 ug) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody used at 0.1 ug/ml. To examine total MCM2, the blot was stripped and then blotted with rabbit anti-MCM2 antibody at 0.1 ug/ml.](http://images.novusbio.com/fullsize/MCM2-[p-Ser53]-Antibody-Western-Blot-NB100-2339-img0003.jpg)