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![Immunoprecipitation:Glycogen Synthase AntibodyNBP3-29755] -Detection of human GYS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-GYS1 antibody NBP3-29755 (lot NBP3-29755-1) used for IP at 6 µg/mg lysate. GYS1 was also immunoprecipitated by rabbit anti-GYS1.. For blotting immunoprecipitated GYS1, NBP3-29755 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29755_rabbit-glycogen-synthase-pab-301220241534310.jpg "Immunoprecipitation:Glycogen Synthase AntibodyNBP3-29755] -Detection of human GYS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-GYS1 antibody NBP3-29755 (lot NBP3-29755-1) used for IP at 6 µg/mg lysate. GYS1 was also immunoprecipitated by rabbit anti-GYS1.. For blotting immunoprecipitated GYS1, NBP3-29755 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48756_rabbit-epithelial-sodium-channel-alpha-mab-hl2967-western-blot-141120251843561.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with epithelial Sodium Channel alpha antibody [HL2967] (NBP3-48756) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48756_rabbit-epithelial-sodium-channel-alpha-mab-hl2967-western-blot-141120251826020.jpg)
![Immunohistochemistry: IkB-beta [p Ser23] Antibody [NBP2-54761] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-B [P-Ser23].](http://images.novusbio.com/fullsize/IkB-beta-[p-Ser23]-Antibody-Immunohistochemistry-NBP2-54761-img0002.jpg "Immunohistochemistry: IkB-beta [p Ser23] Antibody [NBP2-54761] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-B [P-Ser23].")
![Western Blot: Cav1.2 Antibody [NBP1-20896] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Western-Blot-NBP1-20896-img0004.jpg "Western Blot: Cav1.2 Antibody [NBP1-20896] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Serum Response Factor SRF Antibody [NBP1-51976] - Analysis of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA](http://images.novusbio.com/fullsize/Serum-Response-Factor-SRF-Antibody-Western-Blot-NBP1-51976-img0001.jpg "Western Blot: Serum Response Factor SRF Antibody [NBP1-51976] - Analysis of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA")
![Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/JunB-AP-1-Antibody-Western-Blot-NBP1-51950-img0002.jpg "Western Blot: JunB/AP-1 Antibody [NBP1-51950] - Analysis of JunB in Human Breast Cancer lysate (35ug protein in RIPA buffer) using NBP1-51950 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: VASP Antibody [NBP2-42516] - Staining of Human Platelets lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/VASP-Antibody-Western-Blot-NBP2-42516-img0001.jpg "Western Blot: VASP Antibody [NBP2-42516] - Staining of Human Platelets lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Parkin Antibody [NBP1-80515] - Mouse Liver lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Western-Blot-NBP1-80515-img0003.jpg "Western Blot: Parkin Antibody [NBP1-80515] - Mouse Liver lysate, concentration 0.2-1 ug/ml.")
![Western Blot: Rabphilin 3A Antibody [NBP1-69184] - Sample Type: 1. Lamprey CNS (20ug) 2. Rat Brain Lysate (20ug) Primary Dilution: 1:1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary Dilution: 1:4000 Image Submitted by: Jennifer Morgan, PhD & David Busch University of Texas in Austin](http://images.novusbio.com/fullsize/Rabphilin-3A-Antibody-Western-Blot-NBP1-69184-img0002.jpg "Western Blot: Rabphilin 3A Antibody [NBP1-69184] - Sample Type: 1. Lamprey CNS (20ug) 2. Rat Brain Lysate (20ug) Primary Dilution: 1:1000 Secondary Antibody: Goat anti-Rabbit HRP Secondary Dilution: 1:4000 Image Submitted by: Jennifer Morgan, PhD & David Busch University of Texas in Austin")
![Western Blot: Rabphilin 3A Antibody [NBP1-69184] - Rph3a Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Mouse Muscle](http://images.novusbio.com/fullsize/Rabphilin-3A-Antibody-Western-Blot-NBP1-69184-img0001.jpg)
![Western Blot: CBL Antibody [NB100-869] - staining (2 ug/ml) of 3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/CBL-Antibody-Western-Blot-NB100-869-img0002.jpg "Western Blot: CBL Antibody [NB100-869] - staining (2 ug/ml) of 3T3 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Western Blot: Nicotinic Acetylcholine Receptor epsilon Antibody [NBP1-79951] - HepG2 cell lysate, concentration 1.25ug/ml.](http://images.novusbio.com/fullsize/Nicotinic-Acetylcholine-Receptor-epsilon-Antibody-Western-Blot-NBP1-79951-img0003.jpg "Western Blot: Nicotinic Acetylcholine Receptor epsilon Antibody [NBP1-79951] - HepG2 cell lysate, concentration 1.25ug/ml.")
![Bioactivity: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Immobilized Human CD31 at 1 ug/mL (100 uL/well) can bind Human beta-Catenin with a linear range of 0.3-4.9ug/ml.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Catenin-His-C-Term-Protein-Bioactivity-NBP3-18198-img0002.jpg "Bioactivity: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Immobilized Human CD31 at 1 ug/mL (100 uL/well) can bind Human beta-Catenin with a linear range of 0.3-4.9ug/ml.")
![SDS-Page: Recombinant Human beta-Catenin His (C-Term) Protein [NBP3-18198] - Recombinant Human beta-Catenin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100-110 kDa.](http://images.novusbio.com/fullsize/Recombinant-Human-beta-Catenin-His-C-Term-Protein-SDS-Page-NBP3-18198-img0001.jpg)