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![Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48688_rabbit-huntingtin-mab-hl2483-western-blot-141120251840523.jpg "<b>Genetic Strategies Validation. </b>Wild-type (WT) and HTT knockout (KO) 293T cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody [HL2483] (NBP3-48688) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48688_rabbit-huntingtin-mab-hl2483-western-blot-1411202518365910.jpg)
![Immunohistochemistry-Paraffin: RB1 Antibody (rRB1/8060) [NBP3-23828] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with RB1 Antibody (rRB1/8060). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23828_mouse-rb1-mab-rrb1-8060-2412025863671.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (rRB1/8060) [NBP3-23828] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with RB1 Antibody (rRB1/8060). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Western Blot:[NBP3-30366]-Detection of human pBCKDHA by western blot. Samples: Whole cell lysate from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30366_rabbit-bckdha-pab-231202511585516.jpg "Western Blot:[NBP3-30366]-Detection of human pBCKDHA by western blot. Samples: Whole cell lysate from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antibody used for WB at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-1411202518392011.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-14112025184051.jpg)
![Western Blot:[NBP3-30338]-Detection of human and mouse Stathmin by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Stathmin antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30338_rabbit-stathmin-1-pab-23120251252860.jpg "Western Blot:[NBP3-30338]-Detection of human and mouse Stathmin by western blot. Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Stathmin antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Wild-type (WT) and 4EBP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/4EBP1-[p-Ser65]-Antibody-Western-Blot-NBP3-13171-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Wild-type (WT) and 4EBP1 (phospho Ser65) knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: 4EBP1 [p Ser65] Antibody [NBP3-13171] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with 4EBP1 (phospho Ser65) antibody (NBP3-13171) diluted at 1:500.](http://images.novusbio.com/fullsize/4EBP1-[p-Ser65]-Antibody-Western-Blot-NBP3-13171-img0001.jpg)
![Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30522_rabbit-blooms-syndrome-protein-blm-pab-2312025129511.jpg "Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0001.jpg)
![Western Blot: LRP-6 [p Ser1490] Antibody [NBP3-12921] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP-6 (phospho Ser1490) antibody (NBP3-12921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/LRP-6-[p-Ser1490]-Antibody-Western-Blot-NBP3-12921-img0002.jpg "Western Blot: LRP-6 [p Ser1490] Antibody [NBP3-12921] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP-6 (phospho Ser1490) antibody (NBP3-12921) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: LRP-6 [p Ser1490] Antibody [NBP3-12921] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP-6 phospho S1490 antibody (NBP3-12921) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)](http://images.novusbio.com/fullsize/LRP-6-[p-Ser1490]-Antibody-Western-Blot-NBP3-12921-img0001.jpg)
![KISS1 antibody [HL3043] detects secreted KISS1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human placenta.KISS1 stained by KISS1 antibody [HL3043] (NBP3-48764) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48764_rabbit-kiss1-mab-hl3043-immunohistochemistry-paraffin-1411202518345917.jpg "KISS1 antibody [HL3043] detects secreted KISS1 protein by immunohistochemical analysis.Sample: Paraffin-embedded human placenta.KISS1 stained by KISS1 antibody [HL3043] (NBP3-48764) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: PKA R2 [p Ser99] Antibody [NBP3-12928] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody (NBP3-12928) diluted at 1:3000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/GTX130457_41920_20161013_WB_CIP_w_23060523_861_2.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKA R2 [p Ser99] Antibody [NBP3-12928] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKA R2 (phospho Ser99) antibody (NBP3-12928) diluted at 1:3000. The signal was developed with Trident ECL plus-Enhanced.")
