| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-68136SS | |||
| Azide and BSA Free | NBP2-80973 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-892SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13169SS | |||
| Azide and BSA Free | NBP2-80553 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71965 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90304-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74520 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89639-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79836 | |||
![Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Intracellular-NB110-68136-img0010.jpg "<b>Biological Strategies Validation. </b>Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].")
![<b>Biological Strategies Validation. </b>Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Western-Blot-NB110-68136-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunocytochemistry-Immunofluorescence-NB110-68136-img0012.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0009.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-BSA-Free-Flow-Cytometry-NB110-68136-img0016.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Immunohistochemistry-Paraffin-NB110-68136-img0008.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0007.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0011.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0013.jpg)
![Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-4C8MS-Flow-Cytometry-NB110-68136-img0014.jpg)
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0006.jpg "Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: Mouse Pancreas Whole Cell Lysate. Lane 2: HUVEK Whole Cell Lysate.Lane 3: Human rDLL4.Lane 4: HEK293 Whole Cell Lysate + Human rDLL4. Lane 5: HEK293 Whole Cell Lysate.Load: 10 ug per lane for Whole Cell Lysates or 50 ng for recombinant protein.Primary antibody: DLL4 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 74 kDa, 74 kDa for DLL4. Other band(s): Not Identified.")
![Immunohistochemistry: DLL4 Antibody [NB600-892] - Affinity Purified DLL4 antibody was used at 20 ug/ml to detect DLL4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows DLL4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/DLL4-Antibody-Immunohistochemistry-NB600-892-img0007.jpg)
![Western Blot: DLL4 Antibody [NB600-892] - Western Blot of DLL4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 ug per lane.Primary antibody: DLL4 antibody at 1:500 for overnight at 4C.Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time.Block: 5% Goat Serum overnight at 4C.Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NB600-892-img0005.jpg)
![ELISA: DLL4 Antibody [NB600-892] - ELISA results of purified DLL4 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/DLL4-Antibody-ELISA-NB600-892-img0008.jpg)
![Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Western-Blot-NBP2-13169-img0003.jpg "Western Blot: A2BP1 Antibody (D8H8) [NBP2-13169] - Western blot analysis of A2BP1 expression in 1) human brain and 2) mouse brain tissue lysates.")
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody (D8H8) [NBP2-13169] - A2BP1 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Immunocytochemistry-Immunofluorescence-NBP2-13169-img0004.jpg)
![Immunohistochemistry: A2BP1 Antibody (D8H8) [NBP2-13169] - IHC staining of A2BP1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Immunohistochemistry-NBP2-13169-img0005.jpg)
![Simple Western: A2BP1 Antibody (D8H8) [NBP2-13169] - Simple Western lane view shows a specific band for A2BP1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/A2BP1-Antibody-D8H8-Simple-Western-NBP2-13169-img0006.jpg)
![Western Blot: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A2BP1.](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Western-Blot-NBP2-45532-img0015.jpg "Western Blot: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A2BP1.")
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0014.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0001.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0002.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0003.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0004.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Carcinoma of Human liver tissue .(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0005.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0006.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0007.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0008.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0009.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0010.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0011.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0012.jpg)
![Immunohistochemistry: A2BP1 Antibody (4F9) [NBP2-45532] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/A2BP1-Antibody-4F9-Immunohistochemistry-NBP2-45532-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: A2BP1 Antibody [NBP1-90304] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/A2BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90304-img0003.jpg "Immunocytochemistry/Immunofluorescence: A2BP1 Antibody [NBP1-90304] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.")
![Immunohistochemistry-Paraffin: A2BP1 Antibody [NBP1-90304] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/A2BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-90304-img0004.jpg)
![Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Western-Blot-NBP2-45933-img0009.jpg "Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)")
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0005.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0001.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0002.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0003.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0004.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0006.jpg)
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)
![Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.](http://images.novusbio.com/fullsize/Tenascin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-89639-img0002.jpg "Immunohistochemistry-Paraffin: Tenascin C Antibody [NBP1-89639] - Staining of human hippocampus shows distinct positivity in neuropil.")
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0003.jpg "Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).")
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Adrenal stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0001.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0002.jpg)
![Protein Array: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIM-3 Antibody (TIM3/3113) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Protein-Array-NBP2-79702-img0004.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - TIM-3 was stained in human FFPE head and neck squamous cell carcinoma tissue. HIER antigen retrieval for 20min at pH 9. AF750 conjugated version of antibody was used (Catalog # NBP2-79836AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-79702_mouse-monoclonal-tim-3-antibody-tim3-3113-17720241044615.jpg)
![Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/LRFN2-Antibody-Western-Blot-NBP2-81731-img0004.jpg "Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.")
![Western Blot: LRFN2 Antibody [NBP2-81731] - Western blot analysis of LRFN2 expression in mouse brain tissue lysate with LRFN2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/LRFN2-Antibody-Western-Blot-NBP2-81731-img0001.jpg)
![Immunohistochemistry: LRFN2 Antibody - BSA Free [NBP2-81731] - Immunohistochemistry of LRFN2 in mouse brain tissue with LRFN2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81731_rabbit-polyclonal-lrfn2-antibody-2711202420285822.jpg)
![Immunocytochemistry/ Immunofluorescence: LRFN2 Antibody - BSA Free [NBP2-81731] - Immunofluorescence of LRFN2 in mouse brain tissue with LRFN2 antibody at 20 ugmL.](http://images.novusbio.com/fullsize/nbp2-81731_rabbit-polyclonal-lrfn2-antibody-2711202419353857.jpg)
![Western Blot: TIM-3 Antibody [NB100-56422] - Analysis of TIM-3 in (A) mouse and (B) rat kidney cell lysate using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/TIM-3-Antibody-Western-Blot-NB100-56422-img0002.jpg "Western Blot: TIM-3 Antibody [NB100-56422] - Analysis of TIM-3 in (A) mouse and (B) rat kidney cell lysate using this antibody at 2 ug/ml.")
![Western Blot: DLL4 Antibody [NBP2-27407] - Analysis of DLL4 in HUVEC cell lysate using 1) preimmune sera at 1:5000 and 2) NBP2-27407 at 3 ug/ml, and 3) mouse embryo body lysate at 6 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/DLL4-Antibody-Western-Blot-NBP2-27407-img0001.jpg "Western Blot: DLL4 Antibody [NBP2-27407] - Analysis of DLL4 in HUVEC cell lysate using 1) preimmune sera at 1:5000 and 2) NBP2-27407 at 3 ug/ml, and 3) mouse embryo body lysate at 6 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")