| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74240 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88247-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90322-25ul | |||
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0015.jpg "Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0010.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Protein expression analysis of normal and NPC-deficient cells after HIV-1 infection. Cells were uninfected or infected with VSVG-HIV-1 and harvested 96 h post-infection. NPC2, NPC1, and beta-actin protein expression was detected via Western blotting in uninfected and infected cells. Gag expression was also detected in the infected cells. Image collected and cropped by CiteAb from the following publication (//virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-31), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0016.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Immunocytochemical staining with corresponding colocalization analysis of ALIX (green) and NPC-1 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) and merged images show colocalization in yellow (n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick C1 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockout-Validated-NB400-148-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - HeLa lysate, BHK lysate, A431 lysate tested at 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockdown-Validated-NB400-148-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - ESCRT complex recruited to LLOMe-damaged lysosomes promotes cell survival.Human fibroblasts were exposed to 1 mM LLOMe & when indicated, the cell permeable calcium chelator BAPTA-AM (1 µM) was added 10 min prior to LLOMe exposure. Immunocytochemical staining with corresponding colocalization analysis of a CHMP4B (green) & LAMP2 (red), b ALIX (green) & NPC-1 (red), & c CHMP4B (green) & LAMP2 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) & merged images show colocalization in yellow (n = 5). d Caspase-3 like activity in cells exposed to LLOMe for 8 h (n = 3). e Quantification of apoptotic & necrotic cells using Annexin V/PI staining in fibroblasts exposed to LLOMe for 6 h (n = 3). f Immunoblot of LAMP2 in digitonin-extracted cytosolic fractions from cells exposed to LLOMe for 2 h (n = 8) with corresponding quantitative analysis. *p < 0.05, **p < 0.01 & ***p < 0.001 compared to control (a–b) or LLOMe only (c–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416165630.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Expression of proteins involved in extracellular lipid uptake & intracellular cholesterol trafficking following JQ1 (0.4 µM) administration to HepG2 cells for 48 hours. (A) Representative Western blot (left panel) & densitometric analysis (right panel) of SR-B1. n = 6 independent experiments. (B) Immunofluorescence analysis of SR-B1 (green). Nuclei were counterstained with DAPI. n = 3 different experiments. Scale bar: 50 µm. (C) Representative Western blot (left panel) & densitometric analysis (right panel) of LDLr. n = 5 independent experiments. (D) LDLr immunofluorescence (green). Nuclei were counterstained with DAPI. n = 3 different experiments. (E–F) Representative Western blots & densitometric analysis of NPC1 & TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t test. ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32075110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416163786.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Effects of p75NTR modulation by LM11A-31 on cholesterol metabolism & secretion. (A–D) Representative Western blot & densitometric analysis of HMGCR, ABCA1, NPC1, & ApoE in U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 4 different experiments. Vinculin served as a housekeeping protein to normalize protein loading. (E) Quantification of ApoE amount (µg/mL) by ELISA assay in culture medium from vehicle- & LM11A-31-treated U373 cells. n = 3 different experiments. (F) Cholesterol quantification (total cholesterol, free cholesterol, & cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was carried out by using unpaired Student’s t test. *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-31020241616058.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1.](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Western-Blot-NBP2-45889-img0022.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0021.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0001.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0002.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0003.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0004.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0005.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0006.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0007.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0008.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0009.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0010.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0011.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0012.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0013.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0014.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0015.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0016.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0017.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0018.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0019.jpg)
![Immunohistochemistry: SMPD1 Antibody (3H7) [NBP2-45889] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/SMPD1-Antibody-3H7-Immunohistochemistry-NBP2-45889-img0020.jpg)
![SMPD1 Antibody (OTI3H7)[NBP2-45889]Simple Western� analysis of endogenous protein RIOK2 from HELA lysates (0.2 mg/mL) using RIOK2 Mouse Monoclonal Antibody . The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.](http://images.novusbio.com/fullsize/nbp2-45889_mouse-smpd1-mab-oti3h7-simple-western-131120251236221.jpg%253fstore=america&image-type=image)
![Immunocytochemistry/Immunofluorescence: SPFH2 Antibody [NBP1-88247] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88247-img0010.jpg "Immunocytochemistry/Immunofluorescence: SPFH2 Antibody [NBP1-88247] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247] - Staining of human testis shows moderate granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunohistochemistry-Paraffin-NBP1-88247-img0017.jpg)
![Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247] - Staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunohistochemistry-Paraffin-NBP1-88247-img0014.jpg)
![Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunohistochemistry-Paraffin-NBP1-88247-img0015.jpg)
![Immunohistochemistry-Paraffin: SPFH2 Antibody [NBP1-88247] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunohistochemistry-Paraffin-NBP1-88247-img0016.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with Adipose tissue.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0008.jpg "Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with Adipose tissue.")
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of anti-ADRB3 antibody with human adipose.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Brain, Glioblastoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Ovary, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human small intestine tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human prostate tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with adrenal tissue.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0007.jpg)
![Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Western-Blot-H00000155-B01P-img0005.jpg "Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of negative control 293 cells (Black) and ADRB3 expressing 293 cells (Green) using ADRB3 purified MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Flow-Cytometry-H00000155-B01P-img0003.jpg)
![Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Western-Blot-NB100-1884-img0002.jpg "Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SPFH2-Antibody-Immunohistochemistry-Paraffin-NB100-1884-img0003.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0004.jpg "Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.")
![Immunocytochemistry/Immunofluorescence: 5-HT2B Antibody [NBP1-55429] - Spinal Cord, ventral horns of mouse, 1.3ug/mL Image Submitted By: Timur Mavlyutov, PhD Department of Pharmacology University of Wisconsin Medical School](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-55429-img0002.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0001.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0003.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0005.jpg)
![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0004.jpg "Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.")
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0006.jpg)
![Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Flow-Cytometry-NBP2-22365-img0007.jpg)
![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 70kD |Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0002.jpg)
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0008.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0009.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0006.jpg "Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Analysis of anti-5HT2B Receptor antibody with human brain, cortex.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0004.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0005.jpg)