| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91810-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80849-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85793-25ul | |||
![Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91810-img0027.jpg "Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP1-91810] - Staining of human cell line U-251 MG shows localization to nucleoli. Antibody staining is shown in green.")
![Western Blot: CRBN Antibody [NBP1-91810] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0025.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Simple Western lane view shows a specific band for CRBN in 0.2 mg/ml of RT4 (left), U-251MG (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0014.jpg)
![Simple Western: CRBN Antibody [NBP1-91810] - Electropherogram image(s) of corresponding Simple Western lane view. CRBN antibody was used at 1:60 dilution on RT4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/CRBN-Antibody-Simple-Western-NBP1-91810-img0015.jpg)
![<b>Genetic Strategies Validation. </b>Single Cell Western: CRBN Antibody [NBP1-91810] - CRBN expression after Downregulation by siRNA, dilution 1:500. Single Cell Western Image submitted by verified customer review.](http://images.novusbio.com/fullsize/CRBN-Antibody-Single-Cell-Western-NBP1-91810-img0035.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CRBN Antibody [NBP1-91810] - Validation of SALL4 asa bona fide IMiD-dependent CRL4CRBN substrate. Parental Kelly cells or two independent pools of CRBN -/- Kelly cells were treated with increasing concentrations of thalidomide. Following 24 h incubation, SALL4, CRBN, and GAPDH protein levels were assessed by western blot analysis. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30067223/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CRBN-Antibody-Western-Blot-NBP1-91810-img0036.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0005.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of rat hippocampus stained with NBP1-92683 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Western blot of rat olfactory epithelium (OE) and frontal cortex (FC). NBP1-92683 stains a band at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Mixed neuron-glial cultures stained with Adenylate Cyclase 3 antibody (NBP1-92683, red) and Alpha Fodrin antibody (NBP1-92689, green). Note the strong and clean staining of neuronal cilia. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0003.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80849 (A) shows similar protein distribution across tissues to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80849] - Analysis using Anti-RGN antibody NBP1-80849 (A) shows similar pattern to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0019.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0027.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80849] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0004.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0025.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0026.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0028.jpg)
![Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Western-Blot-NBP1-40067-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0003.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Analysis of anti-PTH1R antibody with human liver at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-Paraffin-NBP1-40067-img0001.jpg)
![Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-310202415175227.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-31020241534388.jpg)
![Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Human breast cancer cell line MDA-MB-231 was treated by Stat3 inhibitor and expression of IL-6 was detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: IL-6 Antibody [NBP2-16957] - Recombinant IL6 were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-6-Antibody-Western-Blot-NBP2-16957-img0010.jpg)
![Immunohistochemistry-Paraffin: IL-6 Antibody [NBP2-16957] - IL-6 antibody detects IL-6 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.IL-6 stained by IL-6 antibody (NBP2-16957) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16957_rabbit-polyclonal-il-6-antibody-91020242011469.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: DLX5 Antibody [NBP1-85793] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DLX5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85793-img0010.jpg "Immunocytochemistry/Immunofluorescence: DLX5 Antibody [NBP1-85793] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/DLX5-Antibody-Immunohistochemistry-Paraffin-NBP1-85793-img0014.jpg)
![Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DLX5-Antibody-Immunohistochemistry-Paraffin-NBP1-85793-img0011.jpg)
![Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DLX5-Antibody-Immunohistochemistry-Paraffin-NBP1-85793-img0012.jpg)
![Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-85793] - Staining of human placenta shows weak nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/DLX5-Antibody-Immunohistochemistry-Paraffin-NBP1-85793-img0013.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0006.jpg "Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0004.jpg)
![Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Flow-Cytometry-NBP2-67216-img0001.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0003.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80850] - Analysis using Anti-RGN antibody NBP1-80850 (A) shows similar pattern to independent antibody NBP1-80849 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80850-img0021.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80850] - Analysis using Anti-RGN antibody NBP1-80850 (A) shows similar pattern to independent antibody NBP1-80849 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Regucalcin Antibody [NBP1-80850] - Staining of human cell line Hep G2 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80850-img0008.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550351.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80850 (A) shows similar protein distribution across tissues to independent antibody NBP1-80849 (B).](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550350.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550354.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550355.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550352.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Immunohistochemistry analysis in human adrenal gland and tonsil tissues using NBP1-80850 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550353.jpg)