| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97485-0.2mg | |||
| BSA Free | NBP1-97485-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87842-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59770SS | |||
| Azide and BSA Free | NBP2-80894 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33132 | |||
| IHC-Prediluted | NBP2-44711 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-167SS | |||
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0009.jpg "Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-324] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-324-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-324-img0010.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0004.jpg)
![Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-324-img0007.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97485-img0003.jpg "Western Blot: Calnexin Antibody [NBP1-97485] - Analysis: Lane 1: HeLa (heat shocked), Lane 2: Vero (heat shocked), Lane 3: Rat-2 (heat shocked), Lane 4: L-929 (heat shocked).")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97485] - Analysis of human spleen tissue stained with Calnexin, pAb at 10ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97485-img0002.jpg)
![Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0004.jpg "Western Blot: NF-M Antibody [NB300-222] - Western blot analysis of different neuronal tissue and cell lysates using chicken pAb to NF-M, NB300-222, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH/3T3 cells, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. Strong band at 145kDa corresponds to rodent NF-M, and about 160kDa band corresponds to human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties. NF-M is not expressed in HeLa and other cell lines tested.")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody [NB300-222] - 160kDa Neurofilament Medium Antibody [NB300-222] - View of mixed neuron/glial cultures stained with NB300-222 (red). The NF-M protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.](http://images.novusbio.com/fullsize/160kDa-Neurofilament-Medium-Antibody-Immunocytochemistry-Immunofluorescence-NB300-222-img0002.jpg)
![Immunohistochemistry Free-Floating: NF-M Antibody [NB300-222] - Immunofluorescent analysis of rat cerebellum section stained with chicken pAb to NF-M, NB300-222, dilution 1:1,000 in red, and costained with mouse mAb to CNPase, dilution 1:500, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free floating sections were stained with above antibodies. The NF-M antibody labels the axons of basket cells and other neurons, while the CNP antibody stains oligodendrocytes, cells that form the myelin sheathes around axons.](http://images.novusbio.com/fullsize/NF-M-Antibody-NB300-222-img0003.jpg)
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)
![Western Blot: Synaptojanin 1 Antibody [NBP1-87842] - (A) The levels of synj1 protein in N2a cells after synj1 siRNA. Results are presented as % of levels in controls +/- SEM. (B) A representative example of western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-11654-7) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Knockdown-Validated-NBP1-87842-img0023.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Synaptojanin 1 Antibody [NBP1-87842] - (A) The levels of synj1 protein in N2a cells after synj1 siRNA. Results are presented as % of levels in controls +/- SEM. (B) A representative example of western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-11654-7) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87842-img0022.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0020.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebellum shows moderate positivity in neuropil as well as in a subset of neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0017.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebral cortex shows positivity in neuropil as well as moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0018.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human rectum shows moderate cytoplasmic positivity in neurons in peripheral ganglia.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0019.jpg)
![Western Blot: Synaptojanin 1 Antibody [NBP1-87842] - Reduction of pTau with synj1 knockdown in vitro. (A) The levels of pTau, Tau & synj1 protein in N2a cells after synj1 siRNA. Results are presented as % of levels in controls ± SEM. (B) A representative example of western blot analysis. (C) PIP2 levels. (D) Immunofluorescence staining of pTau (red) & Tau (green) with (E) quantification. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28900205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87842_rabbit-polyclonal-synaptojanin-1-antibody-310202415392594.jpg)
![Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Western-Blot-NBP2-59770-img0001.jpg "Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0022.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunohistochemistry-Paraffin-NBP2-59770-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0002.jpg)
![Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-561-img0023.jpg "Western Blot: FUS Antibody [NB100-561] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-FUS antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.")
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse teratoma Antibody: Affinity purified rabbit anti-FUS (NB100-561) used at 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-561-img0024.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-561] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (NB100-561). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-NB100-561-img0020.jpg)
![Immunoprecipitation: FUS Antibody [NB100-561] - FUS immunoprecipitation in human fibroblast cell lysates. Image from verified customer review.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: FUS Antibody [NB100-561] - Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-561 used for IP at 3 ug per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (NBP2-76415) and rabbit anti-FUS antibody NB100-565. For blotting immunoprecipitated FUS, NBP2-76415 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/FUS-Antibody-Immunoprecipitation-NB100-561-img0021.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0019.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis of NCAM-1/CD56 antibody (123C3.D5) on Human colon tissue. Incubation: 1 ug/ml for 30 minutes at room temperature. Image from verified customer review.")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) orstained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0020.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Immunohistochemistry-Paraffin-NBP2-15184-img0016.jpg)
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Analysis using the Azide and BSA Free version of NBP2-15184. Staining of AF700 conjugated CD56 in human PBMC using anti-CD56 antibody. The primary antibody was used at a dilution of 1:100, incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Flow-Cytometry-NBP2-15184-img0014.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Simple Western lane view shows a specific band for NCAM-1/CD56 in 0.2 mg/ml of IMR-32 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0007.jpg)
![Simple Western: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Electropherogram image of the corresponding Simple Western lane. NCAM-1/CD56 antibody was used at 10 ug/ml dilution of IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-Simple-Western-NBP2-15184-img0012.jpg)
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0012.jpg "Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.")
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0005.jpg)
![Western Blot: SHANK1 Antibody [NB300-167] - Shank1a in rat brain lysate using NB300-167 at 1:500 dilution.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Western-Blot-NB300-167-img0007.jpg)
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT2 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3. Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK1 Antibody [NB300-167] - An ASD biometal profile affects cell health and synapse numbers in vitro. Immunocytochemistry of hippocampal neurons DIV 14 grown under control, ASD1, and ASD2 conditions. The fluorescence intensity of Shank positive puncta was measured using antibodies specific for Shank1, Shank2, and Shank3. Exemplary images (upper panel) and quantification of average puncta signal intensity of 10 cells per condition (lower panel). Merged images show additional DAPI staining of the nucleus. Neurons grown under ASD1 conditions show a significant decrease of synaptic Shank proteins, while neurons under ASD2 conditions did not show a reduction. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/np/2015/985083/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0010.jpg)
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers and the stratum moleculare of the dentate gyrus. Confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 um), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) and SM (3, bottom row) (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK1 Antibody [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white;red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 um), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-167-img0011.jpg)
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1 (white; green in merge) and VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 um), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 um). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0017.jpg)
![Immunohistochemistry: SHANK1 Antibody [NB300-167] - Retinal tissue using NB300-167 at 1:800 dilution. Images are 2048 X 2048, 12 bit, collected with a 40X water objective, 10 stacks 0.55 um per stack.](http://images.novusbio.com/fullsize/SHANK1-Antibody-Immunohistochemistry-NB300-167-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202415291988.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Synaptic distribution of Shank1/2 & somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. Top box: 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar = 300 μm. Bottom box: same stainings as top box but with the VGLUT2 antibody (white; red in merge). Red arrow points toward the VGLUT2-band of the DG; scale bar = 300 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202415304214.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank puncta with VGLUT2 puncta in the VGLUT2-band nor in the intragranular mossy fibers of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge), & VGLUT2 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the outer part of the SG (2, top row) & in the PL (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202416205165.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the stratum radiatum of the CA1. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA1 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR; I./II./III. indicates uppermost/middle/bottom figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202416212374.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank puncta with VGLUT1 puncta in the intragranular mossy fibers & the stratum moleculare of the dentate gyrus. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: dentate Gyrus = DG, containing stratum granulare = SG, stratum moleculare = SM, & polymorphic layer = PL; I. indicates uppermost row/figure in bottom box). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper rows (large squares) show the enlarged region (3 = SM; 2 = SG; 1 = PL; scale bar = 100 μm), the bottom rows (small rectangles) show further enlargements (indicated in upper row by white rectangles) in the PL (1, top row) & SM (3, bottom row) (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202416203722.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - No colocalization of Shank immunoreactivity with VGLUT2 puncta in the VGLUT2-band of the stratum pyramidale nor in the mossy fibers of the CA3. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT2 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows further enlargements (indicated in upper row by white rectangles) in the lateral part of the SP (2, top row) & the SR (1, bottom row) (scale bar = 5 μm). Red arrows indicate VGLUT2-band. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202416205156.jpg)
![Immunocytochemistry/ Immunofluorescence: SHANK1 Antibody - BSA Free [NB300-167] - Colocalization of Shank immunoreactivity with VGLUT1 in the stratum radiatum/stratum lucidum of the CA3 region. Top box: Schematic overview of hippocampus with enlarged region (yellow square; here: CA3 containing stratum pyramidale = SP, stratum oriens = SO & stratum radiatum = SR). Bottom box: confocal immunofluorescence stainings of coronal sections from wild-type mice probed with the Shank1-3 (white; green in merge) & VGLUT1 (white; red in merge) antibodies. The upper row (large squares) shows the enlarged region (3 = SO; 2 = SP; 1 = SR; scale bar = 100 μm), the bottom row (small rectangles) shows a further enlargement (indicated in upper row by white rectangle) in the SR (scale bar = 5 μm). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-167_rabbit-polyclonal-shank1-antibody-310202416203718.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Western-Blot-NBP2-67017-img0007.jpg "Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0004.jpg)
![Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Flow-Cytometry-NBP2-67017-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0003.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67017_rabbit-monoclonal-parkin-antibody-jf82-09-western-blot-1962024104114..jpg)