| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19613SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59770SS | |||
| Azide and BSA Free | NBP2-80894 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33168 | |||
| IHC-Prediluted | NBP2-44590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36573SS | |||
| Azide and BSA Free | NBP2-80951 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32932-0.1mg | |||
| BSA Free | NBP2-32932-0.02mg | |||
| Azide and BSA Free | NBP2-34713-0.2mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-137-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05423-0.025ml | |||
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0012.jpg "Western Blot: GPER/GPR30 Antibody [NBP1-31239] - The MW of GPR30 is estimated to be 42 kDa, but higher MW sizes have been reported due to glycosylation and interaction with other proteins. Bands are identified as glycosylated and nonglycosylated based on reports cited in the text, but could include interaction with other proteins. For each GPER western, the membrane was stripped and reprobed for b-actin as a loading control. Quantitation of GPER was evaluated by summing all immunoreactive bands and dividing by b-actin, then normalizing to HBEC2-KT in each blot. Image collected and cropped by CiteAb from the following publication (biomedcentral.com/1471-2407/12/624), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31239] - Detection of GPER/GPR30 in HUVEC nucleus. Image courtesy of a product review by Dr. Subhadeep Chakrabarti of University of Alberta.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31239-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GPER/GPR30 Antibody [NBP1-31239] - A. 50 ug 293T whole cell lysate/extract. B. 50 ug whole cell lysate/extract of GFP-human GPR30-transfected 293T cells (No boiling). C. 50 ug whole cell lysate/extract of GFP-human GPR30 and GPR30 siRNA-transfected 293T cells (No boiling). 5 % SDS-PAGE GPR30 antibody ) dilution: 1:1000](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Western-Blot-NBP1-31239-img0011.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - AhR & GPR30 receptors are present & functional in the MCF10AT1 cells. (A) RT-qPCR analysis of AhR & GPR30 mRNA expression levels represented in arbitrary units (a.u.) in the MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. Values represent mean ± SD of three independent experiments conducted in triplicate. (B) Representative Western blot analyses from three independent experiments of AhR & GPR30 protein expression in MCF10AT1 & MCF10CA1a.cl1 cells. MCF-7 cells were used as a control. (C) XRE-luciferase activity following 8 h exposure of MCF10AT1 cells to ITE at the indicated concentrations. TCDD 10−7 M was used as a control & results were expressed as % of TCDD 10−7 M activity. ***p < 0.001 in Student t-test. (D) XRE-luciferase activity upon 8 h of exposure to ITE 10−10 M alone or in combination with GNF351 at the indicated concentrations. TCDD 10−7 M was used as a control, & results were expressed as % of TCDD 10−7 M activity. Student t-tests revealed the statistically significant differences between unexposed & exposed cells: ***p < 0.001; & between ITE & ITE+GNF351: ###p < 0.001. Values in (C,D) represent mean ± SD of three independent experiments. (E) Representative Western blot analyses from three independent experiments of the phospho-MAPK/MAPK ratio upon exposure of MCF10AT1 cells to G1 (GPR30 agonist) for the times indicated, in the presence or absence of a 2 h pre-treatment with G15 (GPR30 antagonist). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416171440.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173515.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Human endothelial cells express GPR30 protein in the cell nucleus.(A) Confluent monolayers of HUVECs from 3 different cords were lysed & the protein lysates were immunoblotted for GPR30. alpha -tubulin was used as loading control. (B) HUVEC monolayers at 30–40% confluence were treated with 40 nM siRNA (control or GPR30_4) for 48 hours prior to lysis followed by immunoblotting of the cell lysates for GPR30. alpha -tubulin was used as loading control. Data shown are mean ± SEM of 4 independent experiments. ** & ## indicate p<0.01 compared to untreated & control siRNA-treated cells, respectively. (C) Confluent HUVECs grown on glass coverslips were fixed, permeabilized & immunostained with anti-GPR30 antibody. Nuclei were stained with Hoechst33342 dye. The merged image shows GPR30 (red) & nuclei (blue) in pseudocolor. Representative images from 3 independent experiments are shown. Bar, 20 µm. (D) Confluent HUVECs were lysed & fractionated into cytosolic (C) & nuclear (N) fractions prior to western blotting for eNOS, GPR30, p65, alpha -tubulin & c-Jun. A representative set of images (obtained from different membranes) from 3 independent experiments is shown. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23285008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416175294.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416173516.jpg)
![Western Blot: GPER/GPR30 Antibody [NBP1-31239] - Effects of short-term exposure of BPA, B[a]P, ITE & G1 10−10 M on AIG & MFE are inhibited by siRNA-AhR & siRNA-GPR30. Representative Western blot analysis from three independent experiments of AhR & GPR30 expression in transfected MCF10AT1 cells with (A) siRNA-AhR, (B) siRNA-GPR30 or their scrambled controls. Quantification of protein expression levels was normalized against tubulin expression. (C,D) Secondary mammospheres formation & (E,F) average number of colonies in soft agar, with the following treatments: BPA and/or B[a]P, G1, or ITE, 10−10 M. Cells were transfected with either siRNA-AhR, siRNA-GPR30 or their scrambled controls before being subjected to the treatments. Treatments were maintained throughout the course of experiments. (mean ± SD of 2 independent experiments, in triplicate). ***p < 0.001, *p < 0.05 vs. their respective unexposed; ###p < 0.001 siRNA vs. scrambled in Student t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32670863), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-31239_rabbit-polyclonal-gper-gpr30-antibody-310202416163721.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0010.jpg "Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17beta-estradiol. Lane 3: MCF-7 + 10 uM gamma secretase inhibitor. Load: 35 ug per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80 kDa for Notch1.")
![Immunohistochemistry: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Analysis of Tissue: Exocrine glands of human pancreas Fixation: FFPE Primary antibody: Notch1 antibody at 1:200 Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Immunohistochemistry-NB300-251-img0007.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Notch 1 Antibody [NB 300-251]- at 1:500, against myc-tagged transiently transfected mouse Notch constructs in 293 cells.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0003.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0009.jpg)
![Western Blot: Notch-1 Antibody - (Cleaved N terminal) [NB300-251] - Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Cleaved-N-terminal-Western-Blot-NB300-251-img0011.jpg)
![Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.](http://images.novusbio.com/fullsize/DEC2-SHARP1-Antibody-Western-Blot-NBP1-19613-img0006.jpg "Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.")
![Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody [NBP1-19613] - Staining of SHARP1 in mouse pancreas.](http://images.novusbio.com/fullsize/DEC2-SHARP1-Antibody-Immunohistochemistry-Paraffin-NBP1-19613-img0002.jpg)
![Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of SHARP1 in HeLa whole cell extract.](http://images.novusbio.com/fullsize/DEC2-SHARP1-Antibody-Western-Blot-NBP1-19613-img0003.jpg)
![Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of HeLa (1), Raw 264.7 (2), A431 (3), MCF7 (4), and PC12 (5) using SHARP1 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/DEC2-SHARP1-Antibody-Western-Blot-NBP1-19613-img0004.jpg)
![Simple Western: DEC2/SHARP1 Antibody [NBP1-19613] - Image shows a specific band for SHARP1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DEC2-SHARP1-Antibody-Simple-Western-NBP1-19613-img0005.jpg)
![Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Western-Blot-NBP2-59770-img0001.jpg "Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0022.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunohistochemistry-Paraffin-NBP2-59770-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0002.jpg)
![Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Western-Blot-NBP2-29403-img0001.jpg "Western Blot: S100B Antibody (4C4.9) [NBP2-29403] - Analysis of human brain lysate using S100 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal Antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunocytochemistry-Immunofluorescence-NBP2-29403-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - CODEX multiplexing image of S100b in primary melanoma of the skin. Image from verified customer review.](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0009.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human melanoma stained with S100B antibody(4C4.9).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Confocal Immunofluorescent analysis of A2058 cells using AF488-labeled S100B Monoclonal antibody (4C4.9) (Green). F-actin filaments were labeled with DyLight 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0007.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (4C4.9) [NBP2-29403] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Antibody (4C4.9)](http://images.novusbio.com/fullsize/S100B-Antibody-4C4-9-Immunohistochemistry-Paraffin-NBP2-29403-img0008.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0010.jpg "Western Blot: USP9x Antibody [NBP1-48321] - WT1 is a substrate of pUSP9X (serine 2563) in mitosis. Co-immunoprecipitation of FLAG-tagged WT1 with endogenous USP9X from HEK 293T cells (EV, expression vector). Cells were exposed to nocodazole, collected, lysed (WCE, whole cell extracts), and subjected to anti-FLAG immunoprecipitation before analysis by western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-15059-5), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - Immunocytochemical analysis of USP9X in NTERA-2 cells](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0005.jpg)
![Immunohistochemistry: USP9x Antibody [NBP1-48321] - IHC staining of Usp9x in brain tissue.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunohistochemistry-NBP1-48321-img0006.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in Caco-2 whole cell lysates.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0007.jpg)
![Western Blot: USP9x Antibody [NBP1-48321] - Analysis of USP9X in NIH/3T3 whole cell lysate.](http://images.novusbio.com/fullsize/USP9x-Antibody-Western-Blot-NBP1-48321-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: USP9x Antibody [NBP1-48321] - ICC/IC analysis of USP9x in mouse ES cell line 129. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/USP9x-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-48321-img0009.jpg)
![Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of SLC31A1/CTR1 in MCF7 and HeLa at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti SLC31A1/CTR1 (5E6.1B4) NBP2-36573 at a 1:50 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-36573-img0009.jpg)
![Immunohistochemistry: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - IL-17 induces cellular copper uptake through induction of STEAP4. Western blots analysis for copper trafficking-associated proteins from lysates of mouse colon epithelial organoids treated with different inflammatory cytokines for 8h. Three independent experiments were done and representative blots were shown. Untr untreated. IL-17 primed or unprimed mouse colon organoids were stained for STEAP4 (St4), E-cadherin (E-cad) and SLC31A1/CTR1 and integrin alpha. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14698-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-NBP2-36573-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - STEAP4 promotes colitis-associated colon tumorigenesis. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Western blot analysis of colon tissue lysates from mice treated with DSS water for indicated days. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32060280/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Western-Blot-NBP2-36573-img0011.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a formalin fixed and paraffin embedded tissue section of human urinary bladder transitional cell carcinoma (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal breast tissue from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody generated a very strong cytoplasmic staining in the myo-epithelial cells in the tested section.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0008.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Analysis of a FFPE tissue section of normal skin from a human breast cancer patient (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The antibody developed a strong cytoplasmic staining in the outer layer of stratum spinosum and the cells of stratum granulosum/ granular layer of skin epidermis. The endothelial cells in the dermis of the section also showed positivity for SLC31A1/CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody (5E6.1B4) - BSA Free [NBP2-36573] - Aanalysis of a FFPE tissue section of human small bowel malignant stromal tumor (NBP2-30234) using purified CTR1/SLC31A1 antibody clone 5E6.1B4 at 5 ug/ml concentration. The cancer cells developed moderate to strong positivity for CTR1/SLC31A1 protein.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-5E6-1B4-BSA-Free-Immunohistochemistry-Paraffin-NBP2-36573-img0007.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-paraffin human tonsil (20X) stained with CD34 MAb (QBEnd/10). Image using the standard format of this product.")
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Human placental tissue. Antigen retrieval at pH 9. IF by Cy3 conjugate. Image from verified customer review.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (QBEnd/10) - Azide and BSA Free [NBP2-34713] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD34 MAb (QBEnd/10) Image using the standard format of this product.](http://images.novusbio.com/fullsize/CD34-Antibody-QBEnd-10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34713-img0002.jpg)
![Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NB300-137-img0004.jpg "Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Analysis of the widely used rat pheochromocytoma PC12 cell line stained with rabbit polyclonal Periphren antibody, dilution 1:2000 (Green). Hoechst staining of nuclear DNA (Blue). Peripherin, one of the Class III family of intermediate filament subunit proteins, is a major component of the PC12 cell forming a perinuclear cap, with some filaments visible in the cytoplasm.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0003.jpg)
![Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.](http://images.novusbio.com/fullsize/p_4356adfdef9d7.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NB300-137] - Mixed neuron/glia cultures from newborn rat brain stained with NB300-137 antibody to peripherin (green) and chicken polyclonal antibody to phosphorylated NF-H (red channel). A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain NF-H and not peripherin. The blue channel reveals the localization of DNA.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NB300-137-img0001.jpg)
![Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Western-Blot-NBP1-05423-img0003.jpg "Western Blot: Peripherin Antibody [NBP1-05423] - Analysis of spinal cord tissue lysates (lanes 2-5) and cell lysates (lanes 6 and 7) using chicken pAb to peripherin, NBP1-05423, dilution 1:10,000 in green: [1] protein standard (red), [2] rat, [3] mouse, [4] pig, [5] cow spinal cord; [6] SH-SY5Y, and [7] PC12 cells. The band at 57kDa corresponds to peripherin protein.")
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - Analysis of mixed fibroblast and PC12 pheochromocytoma cell culture stained with chicken pAb to peripherin, NBP1-05423, dilution 1:5,000 in green, and costained with rabbit pAb to vimentin, RPCA-Vim, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. NBP1-05423 antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0005.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (red) in nerve bundles in the human larynx. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Peripherin Antibody [NBP1-05423] - shows rat mixed neuron/glial cultures stained with NBP1-05423, our chicken polyclonal antibody to peripherin (green channel) and rabbit antibody to the neurofilament subunit alpha-internexin NBP1-05207 (green channel). These cultures contain mostly neurons which are rich in alpha-internexin, and a subgroup which have a large amount of peripherin also, such as the prominent cell in the middle of the micrograph. Since this cell expresses large amounts of peripherin and alpha-internexin, the green and red signals superimpose to produce a golden cell. Blue is a DNA stain.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05423-img0001.jpg)
![Immunohistochemistry-Frozen: Peripherin Antibody [NBP1-05423] - Peripherin staining (green) in the mouse brainstem including the spinal trigeminal tract (right side of image) and arching fiber bundle to the solitary nuclear tract (center). Nissl Counterstain. Image from verified customer review.](http://images.novusbio.com/fullsize/Peripherin-Antibody-Immunohistochemistry-Frozen-NBP1-05423-img0006.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Western-Blot-NB300-560-img0004.jpg "Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.")
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-33-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)