| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47970-0.1mg | |||
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50053SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47833-0.1mg | |||
| IHC-Prediluted | NBP2-48236 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78965SS | |||
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0003.jpg "Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-18258-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) alpha-Synuclein Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunohistochemistry-NBP3-18258-img0002.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0008.jpg "Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0007.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: NDUFA5 Antibody [NBP1-33504] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Western-Blot-NBP1-33504-img0004.jpg "Western Blot: NDUFA5 Antibody [NBP1-33504] - Sample (30 ug of whole cell lysate) A: Hep G2 15% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP1-33504] - NDUFA5 antibody detects NDUFA5 protein at mitochondria in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. NDUFA5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Immunohistochemistry-Paraffin-NBP1-33504-img0005.jpg)
![Immunohistochemistry-Paraffin: NDUFA5 Antibody [NBP1-33504] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NDUFA5-Antibody-Immunohistochemistry-Paraffin-NBP1-33504-img0002.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (NBP1-33504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33504_rabbit-polyclonal-ndufa5-antibody-western-blot-261120251933912.jpg)
![Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with NDUFA5 antibody [N1C3] (NBP1-33504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33504_rabbit-polyclonal-ndufa5-antibody-western-blot-2611202518475711.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Immunohistochemistry-Paraffin-NBP2-44893-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) [NBP2-44893] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) [NBP2-44893] - Flow Cytometric Analysis of HEK293 cells using NF-L Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Flow-Cytometry-NBP2-44893-img0002.jpg)
![Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Western-Blot-NBP2-50053-img0002.jpg "Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.")
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, NBP2-50053, dilution 1:500 in green and costained with chicken pAb to lamin A/C, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin AC antibody stains the nuclear lamina and membrane.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - HeLa cell cultures were stained with NBP2-50053 (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, green). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-6C6-Immunocytochemistry-Immunofluorescence-NBP2-50053-img0003.jpg)
![Western Blot: NF-H Antibody (SPM203) [NBP2-44727] - Western Blot Analysis of Human Brain tissue lysate using NF-H MAb (SPM203).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM203-Western-Blot-NBP2-44727-img0002.jpg "Western Blot: NF-H Antibody (SPM203) [NBP2-44727] - Western Blot Analysis of Human Brain tissue lysate using NF-H MAb (SPM203).")
![Immunohistochemistry-Paraffin: NF-H Antibody (SPM203) [NBP2-44727] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (SPM203).](http://images.novusbio.com/fullsize/NF-H-Antibody-SPM203-Immunohistochemistry-Paraffin-NBP2-44727-img0001.jpg)
![Immunohistochemistry: TET1 Antibody [NBP1-78965] - IHC analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TET1-Antibody-Immunohistochemistry-NBP1-78965-img0001.jpg "Immunohistochemistry: TET1 Antibody [NBP1-78965] - IHC analysis of TET1 in mouse spleen using DAB with hematoxylin counterstain.")