| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92684SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38326-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86088-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37476-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97306SS | |||
![Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Affinity purified rabbit anti-BICD1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. BICD1 was also immunoprecipitated by rabbit anti-BICD1 antibody NBP1-78734 which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF-1 alpha and BICD1. Cells were pretreated with MG132 (1 uM) for 30 min prior to hypoxia treatment for 24 h. Co-immunoprecipitation of HIF-1 alpha with IgG and BICD1 were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41418-018-0241-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BICD1-Antibody-Western-Blot-NBP1-78735-img0004.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Role of BICD1 in UCB-MSCs survival under hypoxia. a, b The UCB-MSCs were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment. a Cells were exposed to various durations of hypoxia (0–72 h). Cell viability of UCB-MSCs was measured by WST-1 cell viability assay. n = 8. b Cells were incubated in normoxia or hypoxia conditions for 72 h. Representative images of experimental groups at 0, 24, 48, & 72 h of normoxia or hypoxia incubation are presented. Red-marked cells in representative images indicate PI-positive cells. n = 4. Scale bars are 50 μm (Magnification, × 200). cBICD1 or NT siRNA-transfected cells were incubated in hypoxia for 24 h. Cleaved caspase-9, cleaved caspase-3, & beta -Actin protein expressions were detected by western blot analysis. n = 4. d Cells were transfected with BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 48 h. The percentages of apoptotic cells were analyzed by Annexin V/PI analysis, measured by flowcytometer. Annexin V-positive cells were considered as apoptotic cells. n = 4. Quantitative data are presented as a mean ± S.E.M. All blot images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection. e The UCB-MSCs were transfected with pcDNA3.1/BICD1-cEGFP vector, pcDNA3.1/cEGFP vector, HIF1A siRNA or NT siRNA for 24 h prior to hypoxia treatment for 72 h. Cell viability data are presented as a mean ± S.E.M. n = 5. *p < 0.05 vs. normoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, #p < 0.05 vs. hypoxia control with pcDNA3.1/cEGFP vector & NT siRNA cotransfection, @p < 0.05 vs. hypoxia control with pcDNA3.1/BICD1-cEGFP vector & NT siRNA cotransfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171413.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-310202416171451.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Effect of hypoxia on the interaction between HIF1 alpha & BICD1. a Co-immunoprecipitation of HIF1 alpha , Dynein IC, Importin alpha 3, RanBP2 w/ IgG & BICD1 antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. b Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. Scale bars are 8 μm (Magnification, × 1,000). White arrow heads indicate co-localization of HIF1 alpha w/ BICD1. c Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). n = 6. *p < 0.05 vs. normoxia control. d–f Cells pretreated w/ MG132 (1 μM) for 30 min prior to hypoxia treatment for 24 h. d Co-immunoprecipitation of HIF1 alpha w/ IgG & BICD1 shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. e Cells immunostained w/ HIF1 alpha & BICD1-specific antibodies. White arrow heads indicate co-localization of HIF1 alpha w/ BICD1 in MG132-pretreated UCB-MSCs. Scale bars are 8 μm (Magnification, × 1,000). n = 5. f HIF1 activity measured by dual luciferase reporter assay. n = 6. *p < 0.05 vs. normoxia control w/ MG132 pretreatment. g, h Cells transfected w/ BICD1 or NT siRNA for 24 h prior to hypoxia treatment for 24 h. g Co-immunoprecipitation of Dynein IC w/ IgG & HIF1 alpha antibodies shown in left panel. Total protein expressions in lysate shown in right panel. n = 3. h Interaction between HIF1 alpha & Dynein IC (HIF1 alpha /Dynein IC, red) analyzed by PLA. Scale bars are 8 μm (Magnification, × 1,000). Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control w/ NT siRNA transfection, #p < 0.05 vs. hypoxia w/ NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616184.jpg)
![Western Blot: BICD1 Antibody [NBP1-78735] - Involvement of Akt/GSK3 beta pathway in BICD1-mediated HIF1 alpha nuclear translocation. a–e The UCB-MSCs were transfected with GSK3 beta or NT siRNA for 24 h prior to hypoxia treatment for 24 h. a Co-immunoprecipitation of HIF1 alpha & Dynein IC with IgG & BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. b Interaction between HIF1 alpha & BICD1 (HIF1 alpha /BICD1, red) was analyzed by PLA assay. Scale bars are 8 μm (Magnification, × 1,000). n = 5. c HIF1 alpha , Lamin A/C, & alpha -Tubulin in cytosolic & nuclear fractionized samples were detected by western blot. n = 4. d Cells were immunostained with HIF1 alpha -specific antibody. Scale bars are 8 μm (Magnification, × 1,000). n = 4. e HIF1 activities in NT or GSK3 beta siRNA-transfected cells were analyzed by dual luciferase reporter assay. n = 6. Quantitative data are presented as a mean ± S.E.M. All blots & immunofluorescence images are representative. *p < 0.05 vs. normoxia control with NT siRNA transfection, #p < 0.05 vs. hypoxia with NT siRNA transfection Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30464225), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78735_rabbit-polyclonal-bicd1-antibody-31020241616183.jpg)
![Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Western-Blot-NBP1-92684-img0005.jpg "Western Blot: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of rat whole brain lysates using mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in green: [1] protein standard (red), [2] adult rat brain, [3] embryonic E20 rat brain. Strong bands at 40, 45kDa correspond to DCX protein, detected only in the developing brain.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to Doublecortin (DCX), NBP1-92684, dilution 1:1,000 in red, and costained with chicken pAb to Microtubule Associated Protein2 (MAP2), dilution 1:10,000 in green. The blue is DAPI staining of nuclear DNA. DCX antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while MAP2 antibody stains dendrites and perikarya of mature neurons. Therefore DCX antibody is an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (3E1) [NBP1-92684] - Rat brain neural cultures stained with NBP1-92684 (green), a chicken polyclonal antibody to GFAP (NB300-213, red) and DNA (blue). The NBP1-92684 antibody reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for NBP1-92684. This Doublecortin antibody is therefore an excellent marker of developing neuronal cells.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-3E1-Immunocytochemistry-Immunofluorescence-NBP1-92684-img0003.jpg)
![Western Blot: Musashi-1 Antibody [NBP1-32812] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-Western-Blot-NBP1-32812-img0014.jpg "Western Blot: Musashi-1 Antibody [NBP1-32812] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - Frozen sectioned adult mouse retina. Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-Immunohistochemistry-Frozen-NBP1-32812-img0010.jpg)
![Western Blot: Musashi-1 Antibody [NBP1-32812] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Musashi 1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-Western-Blot-NBP1-32812-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody [NBP1-32812] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32812-img0006.jpg)
![Immunohistochemistry-Frozen: Musashi-1 Antibody [NBP1-32812] - MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-Immunohistochemistry-Frozen-NBP1-32812-img0007.jpg)
![MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MSI1 protein stained by MSI1 antibody [N3C3] (NBP1-32812) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32812_rabbit-polyclonal-musashi-1-antibody-immunocytochemistry-immunofluorescence-2611202518395712.jpg)
![Western Blot: Nucleostemin Antibody [NBP2-38326] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-38326-img0003.jpg "Western Blot: Nucleostemin Antibody [NBP2-38326] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-38326] - Staining of human cell line U-2 OS shows localization to nucleoli & nuclear bodies.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38326-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human testis.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0008.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NBP2-38326] - Staining of human oral mucosa shows strong nuclear/ nucleolar positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NBP2-38326-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human kidney, liver, skin and testis using Anti-GNL3 antibody NBP2-38326 (A) shows similar protein distribution across tissues to independent antibody NBP2-38325 (B).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human skin.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human kidney.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human liver.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86088-img0007.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86088-img0017.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human lung shows weak to moderate positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86088-img0011.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - staining of human cerebral cortex shows moderate to strong positivity in neuropil.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86088-img0010.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86088-img0012.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human pancreas shows weak to moderate membranous positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP1-86088-img0013.jpg)
![Western Blot: GDAP1 Antibody [NBP1-84430] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/GDAP1-Antibody-Western-Blot-NBP1-84430-img0004.jpg "Western Blot: GDAP1 Antibody [NBP1-84430] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue")
![Immunocytochemistry/Immunofluorescence: GDAP1 Antibody [NBP1-84430] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & mitochondria. Antibody staining shown in green.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84430-img0006.jpg)
![Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84430-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GDAP1 Antibody [NBP1-84430] - Staining in human cerebral cortex and pancreas tissues using anti-GDAP1 antibody. Corresponding GDAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GDAP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84430-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0007.jpg "Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed MCF7 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of K562 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0006.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed HeLa cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0001.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed K562 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0002.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed U87 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0003.jpg)
![Protein Array: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI/Quaking Antibody (PCRP-QKI-2F10).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Protein-Array-NBP3-13817-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mouse mAb against Mouse heart (1) lysate.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Western-Blot-NBP2-37476-img0003.jpg "Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mouse mAb against Mouse heart (1) lysate.")
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody (2G5) [NBP2-37476] - Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunocytochemistry-Immunofluorescence-NBP2-37476-img0004.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of kidney cancer tissues using DCX mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunohistochemistry-NBP2-37476-img0007.jpg)
![Flow Cytometry: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Flow-Cytometry-NBP2-37476-img0005.jpg)
![Western Blot: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Western-Blot-NBP2-37476-img0002.jpg)
![Immunohistochemistry-Paraffin: Doublecortin Antibody (2G5) [NBP2-37476] - Analysis of brain tissue tissues using DCX mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-Immunohistochemistry-NBP2-37476-img0006.jpg)
![ELISA: Doublecortin Antibody (2G5) [NBP2-37476] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Doublecortin-Antibody-2G5-ELISA-NBP2-37476-img0001.jpg)
![Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NB100-1569-img0005.jpg "Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0007.jpg)
![Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NB100-1569-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569] - Human skin carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NB100-1569-img0002.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0003.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0008.jpg)
![Immunoprecipitation: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody NB100-1569(lot NB100-1569-3) used for IP at 6 ug per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1569-2). For blotting immunoprecipitated GNL3, NB100-1569 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunoprecipitation-NB100-1569-img0006.jpg)
![Western Blot: PLK5 Antibody [NBP1-97306] - WB analysis of PLK5 in 1. human brain lysate and 2. mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species.](http://images.novusbio.com/fullsize/PLK5-Antibody-Western-Blot-NBP1-97306-img0002.jpg "Western Blot: PLK5 Antibody [NBP1-97306] - WB analysis of PLK5 in 1. human brain lysate and 2. mouse brain lysate. Molecular weight of PLK5 differs between human and mouse species.")
![Immunocytochemistry/Immunofluorescence: PLK5 Antibody [NBP1-97306] - PLK5 antibody was tested in HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLK5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97306-img0005.jpg)
![Immunohistochemistry: PLK5 Antibody [NBP1-97306] - IHC analysis of PLK5 in mouse salivary gland using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PLK5-Antibody-Immunohistochemistry-NBP1-97306-img0006.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0007.jpg "Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.")
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0006.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0001.jpg)
![Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Paraffin-NBP2-20029-img0002.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0005.jpg)
![QKI antibody [N2C3] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: QKI protein stained by QKI antibody [N2C3] (NBP2-20029) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20029_rabbit-polyclonal-qki-quaking-antibody-immunocytochemistry-immunofluorescence-2611202518365916.jpg)