| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51582SS | |||
| Azide and BSA Free | NBP2-80720 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88204-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79854 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33076 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47911-0.1mg | |||
| IHC-Prediluted | NBP2-48363 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41262-0.025mg | |||
![Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0018.jpg "Western Blot: RPSA Antibody [NBP1-33002] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - CA922 xenograft, using RPSA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0012.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0013.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody diluted by 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0014.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Liver protein expression in different experimental groups of Laminin R, AdipoR2 and SIRT1.Western Blotting analysis of protein expression in the liver on different experimental groups of aminin Receptor. Image shows demonstrative bands of the analyzed proteins and respective housekeeping protein (beta-tubulin) in liver. Data are expressed in mean +/- s.e.m. *p<0.05 Control diet and EGCG (CE) group versus Control diet and Water (CW) group. #p<0.05 High-fat diet and EGCG (HFE) group versus HFW group. $p<0.05 HFW versus CW. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0141227), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0015.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - Sample (30 ug of whole cell lysate). A: Jurkat. 12% SDS PAGE. RPSA Antibody diluted at 1:1000](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0016.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/RPSA-Antibody-Western-Blot-NBP1-33002-img0017.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody [NBP1-33002] - Mouse small intestine. RPSA antibody [N1C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/RPSA-Antibody-Immunohistochemistry-Paraffin-NBP1-33002-img0009.jpg)
![Western Blot: RPSA Antibody [NBP1-33002] - RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 ug) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody (NBP1-33002) diluted by 1:1000](http://images.novusbio.com/fullsize/nbp1-33002_rabbit-polyclonal-rpsa-antibody-9102024205675.jpg)
![Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0007.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0001.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human rectum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88204-img0007.jpg "Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0008.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0014.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0011.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0012.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0013.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88204_rabbit-polyclonal-calpain-2-antibody-652025162443.jpg)
![Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Western-Blot-NBP2-79720-img0010.jpg "Western Blot: RPSA Antibody (RPSA/2699) [NBP2-79720] - Western Blot Analysis of human THP-1, U937, SK-BR3, and A431 cell lysates. RPSA Antibody (RPSA/2699).")
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of paraformaldehyde-Raji cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0003.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0008.jpg)
![Flow Cytometry: RPSA Antibody (RPSA/2699) [NBP2-79720] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Flow-Cytometry-NBP2-79720-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: RPSA Antibody (RPSA/2699) [NBP2-79720] - Immunofluorescent staining of HeLa cells using RPSA Antibody (RPSA/2699) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunocytochemistry-Immunofluorescence-NBP2-79720-img0002.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0004.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0005.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0006.jpg)
![Immunohistochemistry-Paraffin: RPSA Antibody (RPSA/2699) [NBP2-79720] - Formalin-fixed, paraffin-embedded human Tonsil stained with RPSA Antibody (RPSA/2699).](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Immunohistochemistry-Paraffin-NBP2-79720-img0007.jpg)
![Protein Array: RPSA Antibody (RPSA/2699) [NBP2-79720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RPSA Antibody (RPSA/2699) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/RPSA-Antibody-RPSA-2699-Protein-Array-NBP2-79720-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89682-img0013.jpg "Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89682_rabbit-polyclonal-ng2-mcsp-antibody-31020241539744.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human placenta shows moderate membranous and cytoplasmic positivity in trophoblast.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0005.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0006.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0007.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0008.jpg)
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0002.jpg "Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Analysis using the PE conjugate of NBP2-29455. Surface Staining of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 5 ul/0.5 ug/10^6 cells. PPI negative cell population was gated for analysis.")
![Flow Cytometry: CD34 Antibody (ICO-115) [NBP2-29455] - Surface flow cytometric analysis of CD34 on KG-1 cells using CD34 antibody (red) and isotype control (green) at 0.5 ug/10^6 cells.](http://images.novusbio.com/fullsize/CD34-Antibody-ICO-115-Flow-Cytometry-NBP2-29455-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)")
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Flow-Cytometry-NBP2-44569-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (HPCA1/1171)](http://images.novusbio.com/fullsize/CD34-Antibody-HPCA1-1171-Immunohistochemistry-Paraffin-NBP2-44569-img0002.jpg)
![Flow Cytometry: CD34 Antibody (HPCA1/1171) [NBP2-44569] - Percent of EPCs (CD133 + CD34 +) reduced in the XYK group detected by flow cytometry. Data are mean +/- SD analyzed by one-way analysis of variance. **P < 0.01. CSDH, chronic subdural hematoma; EPC, endothelial progenitor cell; XYK, Xiaoyukang Jiaonang. PE conjugated CD34 antibody used (NBP2-47911PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32328124/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-44569_mouse-monoclonal-cd34-antibody-hpca1-1171-177202495947.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0005.jpg "Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NB110-74960-img0003.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP2-41262-img0005.jpg "Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.")
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553050.jpg)
![Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-2711202419553036.gif)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004123.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 ug/ml.Red: TRPV4 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41262_rabbit-polyclonal-trpv4-antibody-271120242004135.gif)
