| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54578-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29623SS | |||
| Azide and BSA Free | NBP2-80957 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33134 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50033SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87374-25ul | |||
![Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB300-155-img0004.jpg "Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).")
![Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-NB300-155-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).")
![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of PFA-fixed Raji cells. B220/CD45R antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg)
![Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Western-Blot-NBP2-29623-img0001.jpg "Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.")
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2 protein in brain [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0010.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0011.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunocytochemistry-Immunofluorescence-NBP2-15186-img0009.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Breast cancer tissue stained for Cytokeratin 19 (red) and counter stained with DAPI (blue). IHC-Fr image submitted by a verified customer review. Image using the Janelia Fluor 549, Azide and BSA free format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunohistochemistry-Frozen-NBP2-15186-img0013.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - analysis of Keratin-19 in 1) HepG2 and 2) MCF7 lysate using of Keratin-19 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0003.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunocytochemistry-Immunofluorescence-NBP2-15186-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunohistochemistry-Paraffin-NBP2-15186-img0005.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Simple-Western-NBP2-15186-img0006.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Simple-Western-NBP2-15186-img0007.jpg)
![Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Western-Blot-NBP2-50033-img0003.jpg "Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0003.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Mouse fore brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Rat hind brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0010.jpg)
![Immunohistochemistry-Whole-Mount: SNAP25 Antibody [NBP1-33714] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody diluted at 1:50.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70 C](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-whole-mount-127202384126.jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-12720238431..jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-127202384456..jpg)
![SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunocytochemistry-immunofluorescence-261120251832456.jpg)
![SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-frozen-261120251829497.jpg)
![SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-DRP1-Protein-SDS-Page-H00010059-P01-img0002.jpg "SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.Lane 1: HMGB1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Western-Blot-H00003146-M02-img0015.jpg "Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.Lane 1: HMGB1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-H00003146-M02-img0011.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Immunohistochemistry-Paraffin-H00003146-M02-img0013.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - HMGB1 monoclonal antibody (M02), clone 1D5 Analysis of HMGB1 expression in K-562.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Western-Blot-H00003146-M02-img0014.jpg)
![Sandwich ELISA Capture: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Detection limit for recombinant GST tagged HMGB1/HMG-1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Sandwich-ELISA-Capture-H00003146-M02-img0016.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-132202416173468.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-4320249115619.jpg)
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87374-img0010.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0015.jpg)
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0007.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0012.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0013.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0014.jpg)