| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80849-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86922-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87374-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44055 | |||
| BSA Free | NBP2-52874-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-79127-25ug | |||
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80849 (A) shows similar protein distribution across tissues to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80849] - Analysis using Anti-RGN antibody NBP1-80849 (A) shows similar pattern to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0019.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0027.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80849] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0004.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0025.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0026.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0028.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining in human small intestine and liver tissues . Corresponding TPH1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Human melanoma cell line A375 and 5 patient melanoma tissue samples. 15 ug cell lysate per lane. TPH1 antibody at 1:400, 4C O/N. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Western-Blot-NBP1-86922-img0020.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0019.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0011.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0013.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0015.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody [NBP1-86922] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-Immunohistochemistry-Paraffin-NBP1-86922-img0018.jpg)
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-STX4 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody [NBP1-87374] - Staining of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87374-img0010.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0015.jpg)
![Western Blot: Syntaxin 4 Antibody [NBP1-87374] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Western-Blot-NBP1-87374-img0007.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0012.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0013.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 4 Antibody [NBP1-87374] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-87374-img0014.jpg)
![Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0021.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Western blot using lysates of HeLa parental cell line (WT) and FUS knockout HeLa cell line (KO), collected in RIPA Buffer. Lysates were prepared using 30 ug of protein and the nitrocellulose membrane was probed with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:5000 O/N at 4C, followed by peroxidase-conjugated Anti-Mouse Secondary Antibody and ECL detection. A specific band at ~70 kDa was detected in the FUS WT cell line but is not observed in the FUS KO cell line. The Ponceau stained transfer of the blot is shown to confirm equal protein loading. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0023.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining in human cerebral cortex and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: FUS Antibody (CL0190) [NBP2-52874] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-FUS monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0014.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U2OS cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0005.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa]Lane 2: Human cell line HeLaLane 3: Human cell line A-431Lane 4: Human cell line MCF-7Lane 5: Human cell line U2-OSLane 6: Human cell line Hep-G2](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in A431 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in MCF7 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U251 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0004.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0016.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0017.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0018.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human liver shows only very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0019.jpg)
![Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Western-Blot-NB300-749-img0007.jpg "Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).")
![Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunocytochemistry-Immunofluorescence-NB300-749-img0004.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0006.jpg)
![Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-APS-11-Immunohistochemistry-Paraffin-NB300-749-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0007.jpg "Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific BlueTM.")
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - hPBMC cells were stained with CD45 antibody (HI30) [NBP1-79127] (blue) and a matched isotype control [NBP2-27287] (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Flour 488.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0005.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] Pacific OrangeTM.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0008.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Surface staining of human peripheral blood cells with anti-human CD45 antibody (HI30) [NBP1-79127] purified, GAM-APC.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0009.jpg)
![Flow Cytometry: CD45 Antibody (HI30) [NBP1-79127] - Analysis of PE/Cy7 conjugate of CD45 antibody (HI30) [NBP1-79127]. Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cy7 (blue histogram) or Anti-Human CD45 PE-Cy7 (purple histogram). Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cytometry-NBP1-79127-img0010.jpg)
![Flow (Cell Surface): CD45 Antibody (HI30) [NBP1-79127] - Flow (Cell Surface): Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue). Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/CD45-Antibody-HI30-Flow-Cell-Surface-NBP1-79127-img0011.jpg)
![Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NB110-41528-img0005.jpg "Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control")
![Immunohistochemistry: GAD2/GAD65 Antibody [NB110-41528] - Formalin fixed, paraffin-embedded Rat pancreas tissue sections were stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit followed by 20 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 20 ug/mL ExtrAvidin-Peroxidase.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-NB110-41528-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB110-41528] - GAD2/GAD65 labelling in Golden hamster hippocampal fomation. Inset: hilus & dentate gyrus. Image from verified customer review.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NB110-41528-img0003.jpg)
![Western Blot: TRPA1 Antibody [NB100-91319] - Tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Western-Blot-NB100-91319-img0001.jpg "Western Blot: TRPA1 Antibody [NB100-91319] - Tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:6000 incubated overnight at 4C.")
![Western Blot: TRPA1 Antibody [NB100-91319] - TRPA1 inhibitor HC030031 significantly ameliorated the oxidative stress & apoptosis levels of Pg.LPS-induced PDLCs. (a) P.g.LPS increased intracellular calcium ion level in a dose-dependent manner (n = 3). (b) Lower HC030031 concentration significantly reversed the increase of intracellular calcium ions, & 10 μM HC030031 was used in the subsequent experiment (n = 3). (c) Flow cytometry analysis of the control group (PDLCs only, C), L group (PDLCs treated by LPS, L), & LH group (PDLCs treated by 10 μM HC030031 & LPS, LH) (n = 4). (d, e) Western blot analysis & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 proteins in in C, L, & LH groups (n = 3). (f) EM images showing endoplasmic reticulum (yellow arrows) & mitochondrial morphology (red arrows) of PDLCs in Ctr, LPS, & LH groups (white stars represent cell nuclei) (n = 4). (g) Quantification of endoplasmic reticulum size, mitochondrial size, mitochondrial number per cell (n = 4), & mitochondrial crista density was analyzed (>100 mitochondria). Data analysis was performed by using one-way ANOVA (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). Data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-91319_rabbit-polyclonal-trpa1-antibody-31020241628499.jpg)
![Western Blot: TRPA1 Antibody [NB100-91319] - Periodontitis-derived periodontal ligament cells & tissues were at high levels of oxidative stress & apoptosis. (a) The related gene expressions of oxidative stress, apoptosis, & some of the TRP families in healthy & periodontitis-derived periodontal ligament cells (PDLCs & P-PDLCs) (n = 3). (b, c) Western blot & semiquantitative statistical analysis of oxidative stress, apoptosis, & TRPA1 in PDLCs & P-PDLCs. (n = 3). d, Flow cytometry analysis of PDLCs & P-PDLCs (n = 4). (e, f) H&E staining (white star represent immune cell infiltration), immunohistochemistry & immunofluorescence staining, & semiquantitative statistical analysis of periodontitis & healthy derived periodontal ligament tissues (PDLTs & P-PDLTs) (n = 3). Data analysis was performed by using Student's t-test (∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001). The data are presented as the mean ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35720191), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-91319_rabbit-polyclonal-trpa1-antibody-310202416284922.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Western blot analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Western-Blot-NBP2-66972-img0009.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Western blot analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - ICC staining of Neutrophil Elastase in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0010.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using anti-Neutrophil Elastase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0011.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of HepG2 cells with Neutrophil Elastase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibod](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Flow-Cytometry-NBP2-66972-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of Neutrophil Elastase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysateLane 2: Mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Western-Blot-NBP2-66972-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Staining Neutrophil Elastase in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunocytochemistry-Immunofluorescence-NBP2-66972-img0004.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of paraffin-embedded human spleen tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0005.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Analysis of paraffin-embedded human tonsil tissue using anti-Neutrophil Elastase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0006.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (JF098-6) [NBP2-66972] - Immunohistochemical analysis of paraffin-embedded mouse bone tissue using anti-Neutrophil Elastase/ELA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neutrophil-Elastase-ELA2-Antibody-JF098-6-Immunohistochemistry-Paraffin-NBP2-66972-img0007.jpg)
![Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Western-Blot-NBP1-40067-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0003.jpg)
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Analysis of anti-PTH1R antibody with human liver at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-Paraffin-NBP1-40067-img0001.jpg)
![Immunohistochemistry: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0181597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-3D1-1-Immunohistochemistry-NBP1-40067-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-310202415175227.jpg)
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (3D1.1) [NBP1-40067] - Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type & Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type & Ramp2-/- placentas. (C & D), Immunofluorescence for PTH1R in the labyrinth of wild type & Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28727763), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-40067_mouse-pth1r-pthr1-mab-3d1-1-31020241534388.jpg)