| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92690SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86169-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44244 | |||
| BSA Free | NBP2-61431-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43806 | |||
| BSA Free | NBP2-46644-25ul | |||
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0006.jpg "Flow Cytometry: CD45 Antibody [NBP2-15811] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50.")
![Flow Cytometry: CD45 Antibody [NBP2-15811] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4 C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody [NBP2-15811], dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488- Conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/CD45-Antibody-Flow-Cytometry-NBP2-15811-img0005.jpg)
![Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Western-Blot-NBP1-92690-img0002.jpg "Western Blot: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of different tissue and cell lysates using mouse mAb to galectin-3, NBP1-92690, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse brain lysate, rat tissue lysates: [3] brain, [4] liver, [5] heart, [6] kidney, [7] lung, and [8] HEK293 cell lysate. The band at about 30kDa mark corresponds to the galectin-3 protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:20,000, in red, which reveals HSP60 protein in all preparations.")
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - MDA-MB-231 cells stained for Gal-3 (red, arrows), shown with DAPI counterstained nuclei (blue). Negative control received non-specific antibody instead of primary antibody. Image from a verified customer review.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (5C21) [NBP1-92690] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to galectin-3, NBP1-92690, dilution 1:1,000 in red, and costained with chicken pAb to MAP2, dilution 1:10,000 in green. The blue is Hoechst staining of nuclear DNA. The NBP1-92690 antibody produces strong staining of microglia (top panel), other glial cells (bottom panel) and lymphocytic cells (small round cells), while the MAP2 antibody labels dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-5C21-Immunocytochemistry-Immunofluorescence-NBP1-92690-img0001.jpg)
![Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Western-Blot-NBP1-20149-img0013.jpg "Western Blot: KCNJ10 Antibody [NBP1-20149] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:3000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0012.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0002.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of mouse kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer from following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0006.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0007.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat kidney. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0009.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat heart. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0010.jpg)
![Immunohistochemistry-Paraffin: KCNJ10 Antibody [NBP1-20149] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen: Candela DAB chromogen from Osenses. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-Immunohistochemistry-Paraffin-NBP1-20149-img0011.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-Paraffin-NB110-93609-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.")
![Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-NB110-93609-img0001.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93609_rat-monoclonal-cd45-antibody-5c16-310202415171311.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in transfected 293T cell line by MEOX2 monoclonal antibody (M03), clone 6A5. Lane 1: MEOX2 transfected lysatE (33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0017.jpg "Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in transfected 293T cell line by MEOX2 monoclonal antibody (M03), clone 6A5. Lane 1: MEOX2 transfected lysatE (33 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of monoclonal antibody to MEOX2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Immunocytochemistry-Immunofluorescence-H00004223-M03-img0010.jpg)
![Immunohistochemistry-Paraffin: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Immunohistochemistry-Paraffin-H00004223-M03-img0011.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0012.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0013.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0014.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0015.jpg)
![Western Blot: MEOX 2 Antibody (6A5) [H00004223-M03] - Analysis of MEOX2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-Western-Blot-H00004223-M03-img0016.jpg)
![ELISA: MEOX 2 Antibody (6A5) [H00004223-M03] - Detection limit for recombinant GST tagged MEOX2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MEOX-2-Antibody-6A5-ELISA-H00004223-M03-img0009.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0010.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0007.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0009.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of A549 cells. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebellum shows strong positivity in both neuronal cell bodies and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex, colon, epididymis and testis using Anti-GSS antibody NBP2-57177 (A) shows similar protein distribution across tissues to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-57177] - Analysis using Anti-GSS antibody NBP2-57177 (A) shows similar pattern to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-57177-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57177-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human testis.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human colon.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human epididymis using Anti-GSS antibody NBP2-57177.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0013.jpg)
![Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518384124.jpg "<b>Genetic Strategies Validation. </b>Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunohistochemistry-paraffin-141120251835222.jpg)
![NDUFS3 antibody [HL2097] detects NDUFS3 protein at mitochondria by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunocytochemistry-immunofluorescence-141120251830215.jpg)
![Whole zebrafish extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-14112025183207.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518415211.jpg)
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-1411202518363616.jpg)
![NDUFS3 antibody [HL2097] detects NDUFS3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.NDUFS3 stained by NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-immunohistochemistry-paraffin-1411202518415222.jpg)
![Whole Japanese medaka extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody [HL2097] (NBP3-48671) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48671_rabbit-ndufs3-mab-hl2097-western-blot-141120251829415.jpg)
![Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-CL3004-Western-Blot-NBP2-46644-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glutamine Synthetase Antibody (CL3004) [NBP2-46644] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GLUL antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0006.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0007.jpg)
![Western Blot: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Western-Blot-NBP2-46644-img0008.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of mouse dentate gyrus shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0003.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0004.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (3004) [NBP2-46644] - Staining of human cerebellum shows positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-3004-Immunohistochemistry-NBP2-46644-img0005.jpg)