| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58346SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44999SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23489SS | |||
| Azide and BSA Free | NBP2-80702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33186 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25146SS | |||
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0018.jpg "Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/RelA/NFkB p65 p65 antibody (27F9.G4) was used to detect p65 by Western blot. Samples were prepared in RIPA lysis buffer, boiled with NuPage 4x LDS Sample Buffer and run on NuPage 4-12% Bis-Tris Gels. Blot was incubated with primary antibody at a dilution of 1:500 and detected with HRP conjugated anti-mouse antibody at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Tissue: Human Fibroblasts Top: Before activation Bottom: After activation with poly IC Left: anti p65 NLS specific Right: Monoclonal antibody C-Term The two antibodies that are shown target different regions of the p65 protein. The different staining patterns are thought to correspond with different functional regions of the protein.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0020.jpg)
![Immunohistochemistry: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. Showed moderate to strong staining within many tissues, including epithelium of the breast, small intestine, kidney, pancreas, prostate, skin, placenta, and uterus, as well as within neurons and lymphoid tissues such as spleen, thymus, and tonsil. The antibody produced an excellent signal with almost no background staining at a concentration of 2.5ug/ml. The image displayed shows specific staining in colon carcinoma as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunohistochemistry-NBP1-77815-img0019.jpg)
![Western Blot: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TBST for 30 min at RT. Blot was probed with monoclonal anti-NFkB p65 at 1:1000 in 1% BSA-TBST at 4C and detected with HRP conjugated Rb-anti-Mouse antibody at 1:40,000 in for 30 min at RT.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Western-Blot-NBP1-77815-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Used at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - Mouse endothelial cells. Unstimulated control cells (left) show cytoplasmic staining, TNF-alpha stimulated cells (right) show nuclear staining. For staining, cells were washed with PBS to remove all traces of culture media and fixed with paraformaldehyde 4 % (45 min). Slides were washed with PBT (phosphate buffer 0.1M + Triton-X-100 0.1 %), 3 times 10 min each, then, blocked with PBT + 5 % normal goat serum, 1 hour. Sample was Incubated overnight in primary antibody (1:600 in blocking buffer). After 3X wash in PBT for 10 min, slides were incubated 1 hour with secondary antibody (1:1000) and mounted in 1:1 PB glycerol.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody (27F9.G4) [NBP1-77815] - RelA/NFkB p65 antibody (27F9.G4) was used to detect p65 by immunofluorescence at a dilution of 1:5000. Hela cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and TritonX 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in widefiled, confocal and STED CW imaging on the same system.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-Antibody-27F9-G4-Immunocytochemistry-Immunofluorescence-NBP1-77815-img0015.jpg)
![Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart](http://images.novusbio.com/fullsize/UCP3-Antibody-Western-Blot-NBP2-24608-img0001.jpg "Western Blot: UCP3 Antibody [NBP2-24608] - Analysis of UCP3 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart and 4) rat heart")
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0010.jpg "Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42847-img0011.jpg)
![Immunohistochemistry-Frozen: GAP-43 Antibody [NBP2-42847] - GAP-43 antibody detects GAP-43 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: GAP-43 protein stained by GAP-43 antibody diluted at 1:250. Red: NeuN, stained by NeuN antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Frozen-NBP2-42847-img0007.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0006.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0008.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody diluted at 1:30000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Western-Blot-NBP2-42847-img0009.jpg)
![Immunohistochemistry-Paraffin: GAP-43 Antibody [NBP2-42847] - Cal27 xenograft. GAP43 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAP-43-Antibody-Immunohistochemistry-Paraffin-NBP2-42847-img0002.jpg)
![Western Blot: GAP-43 Antibody [NBP2-42847] - Human tissue extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GAP-43 antibody (NBP2-42847) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42847_rabbit-polyclonal-gap-43-antibody-91020242021945.jpg)
![Western Blot: Spectrin beta 3 Antibody [NB110-58346] - Detection of Human Spectrin beta 3 on HeLa whole cell lysates using NB110-58346. Spectrin beta 3 was also immunoprecipitated by rabbit anti-Spectrin beta 3 antibody NB110-58783.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Western-Blot-NB110-58346-img0003.jpg "Western Blot: Spectrin beta 3 Antibody [NB110-58346] - Detection of Human Spectrin beta 3 on HeLa whole cell lysates using NB110-58346. Spectrin beta 3 was also immunoprecipitated by rabbit anti-Spectrin beta 3 antibody NB110-58783.")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NB110-58346] - Detection of Spectrin beta 3 in human prostate epithelium.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NB110-58346-img0002.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Western-Blot-NBP1-44999-img0009.jpg "Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on human brain lysate.")
![Immunocytochemistry/Immunofluorescence: HOMER1 Antibody [NBP1-44999] - Detection of HOMER1 (Green) in Hela cells using NBP1-44999. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-44999-img0007.jpg)
![Western Blot: HOMER1 Antibody [NBP1-44999] - Analysis of HOMER1 on mouse brain lysate.](http://images.novusbio.com/fullsize/HOMER1-Antibody-Western-Blot-NBP1-44999-img0008.jpg)
![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0006.jpg "Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE gel, diluted at 1:500.")
![Immunohistochemistry-Frozen: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Representative dual-immunofluorescence staining of rat endothelial cell antigen-1 (RECA-1) and carboxypeptidase E (CPE) in rat heart frozen sections. Image from verified customer review.](http://images.novusbio.com/fullsize/Carboxypeptidase-E-CPE-Antibody-Immunohistochemistry-Frozen-NBP2-15699-img0008.jpg)
![Western Blot: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Carboxypeptidase-E-CPE-Antibody-Western-Blot-NBP2-15699-img0007.jpg)
![Immunohistochemistry-Paraffin: Carboxypeptidase E/CPE Antibody [NBP2-15699] - Human breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Carboxypeptidase-E-CPE-Antibody-Immunohistochemistry-Paraffin-NBP2-15699-img0004.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0003.jpg "Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mouse mAb in (1) A549 and (2) Jurkat cell lysates.")
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human cerebellum tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0005.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in human DRP1 recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0001.jpg)
![Western Blot: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis using DRP1 mAb in (1) HEK293 and (2) DRP1 hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Western-Blot-NBP2-23489-img0002.jpg)
![Immunohistochemistry: DRP1 Antibody (4E11B11) [NBP2-23489] - Analysis of paraffin-embedded human ovarian cancer tissues using DRP1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/DRP1-Antibody-4E11B11-Immunohistochemistry-NBP2-23489-img0004.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Western-Blot-NB110-58874-img0006.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of different tissue and cell lysates using rabbit pAb to UCHL1, NB110-58874, dilution 1:2,000 in red, and mouse mAb to HSP60, dilution 1:10,000, in green: [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The single band at 24kDa corresponds to the UCHL1 protein, while the 60kDa band represents HSP60 protein. UCHL1 is detectable in CNS extracts and cells with neuronal properties but not in HeLa, NIH-3T3 and other non-neuronal cells.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB110-58874] - Analysis of SH-SY5Y cells stained with rabbit pAb to UCHL1, NB110-58874, dilution 1:1,000 in green, and costained with mouse mAb to fibrillarin, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The UCHL1 antibody produces strong staining of the cellular cytoplasm of these cells which share many properties with neurons, while the fibrilarin antibody specifically labels nucleoli.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0005.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Blot of whole bovine brain extract stained with RPCA-UCHL1 showing a strong and clean band at about 24kDa.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB110-58874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Shows rat mixed neuron/glial cultures stained with Rabbit anti-UCHL1 antibody (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58874-img0003.jpg)
![Simple Western: PGP9.5 / UCHL-1 Antibody [NB110-58874] - Simple Western lane view shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB110-58874-img0004.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Western-Blot-NBP2-29420-img0007.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (13C4)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Immunocytochemistry-Immunofluorescence-NBP2-29420-img0006.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0001.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody (13C4) [NBP2-29420] - Electropherogram image of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-13C4-Simple-Western-NBP2-29420-img0004.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Immunofluorescence-NBP2-25146-img0010.jpg "Immunohistochemistry: alpha-Synuclein Antibody [NBP2-25146] - Immunofluorescent analysis of a section of rat cerebellum stained with chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:3,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while GFAP antibody stains the network of Bergmann and astroglial cells.")
![Western Blot: alpha-Synuclein Antibody [NBP2-25146] - Western blot analysis of different tissue lysates using chicken pAb to alpha-synuclein, NBP2-25146, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. The strong band at about 15kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-Western-Blot-NBP2-25146-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 (NBP2-25156, green). The alpha-Synuclein antibody stains vesicular structures, the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-Synuclein than others. The blue channel shows the localization of DNA.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 (1:1000) secondary antibody. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics). Image from verified customer review.](http://images.novusbio.com/fullsize/Synuclein-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-25146-img0006.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0006.jpg "Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - analysis of CACNB2 in HEK293 and CHO whole cell lysates using anti-CACNB2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0004.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0005.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Primary antibody dilution-1:1000 in blocking buffer, 5% Milk in TBS-0.05% Tween for 2 hours at RT. Blocking Overnightat +4 degreeC 2 Hours at RT. Secondary antibody: 1:2000 in blocking buffer, 1 hourat RT. Image from verified customer review.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-Western-Blot-H00000783-M05-img0007.jpg)
![ELISA: CACNB2 Antibody (6C4) [H00000783-M05] - Detection limit for recombinant GST tagged CACNB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CACNB2-Antibody-6C4-ELISA-H00000783-M05-img0003.jpg)