| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84827-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41304-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33060 | |||
| IHC-Prediluted | NBP2-44830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85802-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87499-25ul | |||
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84827 (A) shows similar protein distribution across tissues to independent antibody NBP1-84826 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cell line MCF7 shows localization to nucleus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84827-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0024.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0022.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84827] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84827-img0023.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0005.jpg "Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Western blotting analysis detects Cx26 using R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. (a) R(alpha)Cx26-Ct (A) and R(alpha)Cx26-cl (B) antibodies detected a specific 26kDa band on pig heart tissue lysates (lanes A and B are cropped blots obtained from the same gel). R(alpha)Cx26-cl recognized the 26kDa band on human and rat heart (H) lysates and on rat liver (L) sample (positive control) although two unspecific bands at molecular weights lower than 26kDa were detected. No bands were detected on smooth muscle (SM) tissue lysates (negative control) by R(alpha)Cx26-cl. R(alpha)GAPDH was used as protein loading control for rat tissue samples run on the same gel. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41304-img0002.jpg)
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Human and rat cardiomyocytes immunoreact with R(alpha)Cx26 antibodies. Representative images of immunohistochemical analysis for Cx26 by R(alpha)Cx26-Ct or R(alpha)Cx26-cl antibodies. Longitudinal (c and d) sections showing positivity in cardiomyocytes and in smooth muscle cells of vessel (arrow); Rat (d) immunoperoxidase shows brown immunoreaction in the cell cytoplasm of working cardiomyocytes and in smooth muscle cells of vessels (arrows); positivity of cardiomyocytes and smooth muscle cells has shown, at higher magnification, in longitudinal (d) and cross sections (c); Negative controls are shown in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0004.jpg)
![Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0001.jpg)
![Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0003.jpg)
![Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NB110-75039-img0001.jpg "Western Blot: c-Fos Antibody [NB110-75039] - Western blot on marmoset brain lysate prepared in RIPA buffer containing 1% Triton-X100; 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0003.jpg)
![Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC-P on paraffin sections of mouse brain. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 1: 1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Immunohistochemistry-Paraffin-NB110-75039-img0002.jpg)
![Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Knockout-Validated-NBP2-29433-img0003.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Vimentin Antibody (VM452) [NBP2-29433] - Western blot shows lysates of K562 human Chronic Myelogenous Leukemia parental cell line and Vimentin knockout (KO) K562 cell line. PVDF membrane was probed with 0.7 ug/mL of Mouse Anti-Human Vimentin Monoclonal Antibody (Catalog # NBP2-29433) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Vimentin at approximately 55 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0008.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VM452) [NBP2-29433] - Formalin-paraffin human melanoma stained with Vimentin Ab (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Immunohistochemistry-Paraffin-NBP2-29433-img0002.jpg)
![Flow Cytometry: Vimentin Antibody (VM452) [NBP2-29433] - Flow Cytometry of human Vimentin on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Flow-Cytometry-NBP2-29433-img0004.jpg)
![Western Blot: Vimentin Antibody (VM452) [NBP2-29433] - Western Blot Analysis Raji cell lysate Vimentin Antibody (VM452).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Western-Blot-NBP2-29433-img0007.jpg)
![Protein Array: Vimentin Antibody (VM452) [NBP2-29433] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin Antibody (VM452). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Vimentin-Antibody-VM452-Protein-Array-NBP2-29433-img0006.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NB100-81909-img0010.jpg "Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of extracts from MCF7 cells using NB100-81909 and the same antibody preincubated with blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - ICC/IF showing estrogen receptor alpha (green) in human cervical stromal cells. Nuclei are stained with DAPI (blue). Antibody at 1:200 in 3% BSA. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81909-img0009.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-81909 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NB100-81909-img0006.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of rat-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241523500.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-2342024155515.jpg)
![Immunocytochemistry/ Immunofluorescence: ER alpha/NR3A1 Antibody [NB100-81909] - Immunofluorescence analysis of Hela cells.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414521010.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NB100-81909] - Western blot analysis of lysates from 293 cells, using NB100-81909. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-234202414462211.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NB100-81909] - Immunohistochemical analysis of paraffin-embedded Human-lung tissue.](http://images.novusbio.com/fullsize/nb100-81909_rabbit-polyclonal-er-alpha-nr3a1-antibody-23420241521260.jpg)
![Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-47365-img0003.jpg "Western Blot: MT-ND4 Antibody [NBP2-47365] - Analysis in human cell line HeLa.")
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Stomach shows strong granular cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0011.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Lymph node shows very weak granular cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0009.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-47365] - Staining of human Salivary gland shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunohistochemistry-Paraffin-NBP2-47365-img0010.jpg)
![Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-85519-img0018.jpg "Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-85519] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85519-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0017.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Western-Blot-H00002744-M01-img0001.jpg "Western Blot: Glutaminase Antibody (5C4) [H00002744-M01] - Western Blot analysis of GLS expression in Hela NE ( Cat # L013V3 ).")
![Immunocytochemistry/Immunofluorescence: Glutaminase Antibody (5C4) [H00002744-M01] - Analysis of monoclonal antibody to GLS on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-Immunocytochemistry-Immunofluorescence-H00002744-M01-img0004.jpg)
![ELISA: Glutaminase Antibody (5C4) [H00002744-M01] - Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glutaminase-Antibody-5C4-ELISA-H00002744-M01-img0006.jpg)
![Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Western-Blot-NBP1-87499-img0011.jpg "Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87499-img0015.jpg)
![Western Blot: GABA-AR alpha 3 Antibody [NBP1-87499] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Western-Blot-NBP1-87499-img0010.jpg)
![Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse ventral pallidum shows strong labeling of synaptic fields.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-NBP1-87499-img0004.jpg)
![Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse olfactory bulb shows labelling of both neurons and synapses.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-NBP1-87499-img0005.jpg)
![Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse insular cortex shows strong immunoreactivity in synapses.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-NBP1-87499-img0006.jpg)
![Immunohistochemistry: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of mouse thalamus shows strong positivity in the paraventricular thalamic nucleus.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-NBP1-87499-img0007.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in neuropil.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87499-img0013.jpg)
![Immunohistochemistry-Paraffin: GABA-AR alpha 3 Antibody [NBP1-87499] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GABA-AR-alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP1-87499-img0014.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432833.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249415999.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-212024943553.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374992.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374949.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432886.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249342758.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded A375 xenograft . MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249362712.jpg)
![Frozen-sectioned mouse cerebellum.Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] diluted at 1:100.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-immunohistochemistry-frozen-119202513498.png)
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-141120251843244.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-1411202518433316.jpg)
![<b>Independent Antibodies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-14112025183376.jpg)