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![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-132202416102266.jpg "ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Samples were spiked with high concentrations of Human NF-L and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human NF-L ELISA Kit (Colorimetric) [NBP2-81184] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81184_human-nf-l-elisa-kit-colorimetric-432024941537.jpg)
![Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Titration: 1 ug/ml Positive Control: Fetal kidney lysate.](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-Western-Blot-NBP1-74127-img0006.jpg "Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Titration: 1 ug/ml Positive Control: Fetal kidney lysate.")
![Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Human Brain, cortex tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/Hexosaminidase-A-HEXA-Antibody-Immunohistochemistry-Paraffin-NBP1-74127-img0005.jpg)
![Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-27820241631618.jpg "Western Blot: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Western blot analysis of MDM2/HDM2 on different lysates with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 10uM NUTLIN 3A for 24 hours cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 55 kDa Observed band size: 90 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32584) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunohistochemical analysis of paraffin-embedded human embryonal carcinoma tissue with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32584) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-298202414304162.jpg)
![Immunocytochemistry/ Immunofluorescence: MDM2/HDM2 Antibody (A10E11-R) [NBP3-32584] - Immunocytochemistry analysis of U-2 OS cells treated with or without 10μM NUTLIN 3A for 24 hours labeling MDM2/HDM2 with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MDM2/HDM2 antibody (NBP3-32584) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32584_mouse-mdm2-hdm2-mab-a10e11-r-29202410484835.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [582].")
![<b>Genetic Strategies Validation. </b>Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract | D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0001.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0002.jpg)
![Western Blot: SOD2/Mn-SOD Antibody (582) [NBP2-43795] - Analysis of A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [582] dilution: 1:5000.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-582-Western-Blot-NBP2-43795-img0003.jpg)
![Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32121-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0004.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0003.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Recombinant Nicotinic Acetylcholine R alpha 3/CHRNA3, Human.](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-61220242117596.jpg "Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Recombinant Nicotinic Acetylcholine R alpha 3/CHRNA3, Human.")
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Mouse Cerebrum lysate; Primary Ab: 5ug/ml NBP3-41285; Second Ab: 0.2ug/mLÂ HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-612202421373076.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP3-41285] - Sample: Mouse Cerebellum lysate; Primary Ab: 5ug/ml NBP3-41285; Second Ab: 0.2ug/mLÂ HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41285_rabbit-nicotinic-acetylcholine-r-alpha-3-chrna3-pab-612202421262975.jpg)
![Western Blot: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Analysis of extracts (10ug) from 10 Human tissue by using anti-CDH13 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Cadherin-13-Antibody-OTI3H6-Western-Blot-NBP2-02528-img0008.jpg "Western Blot: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Analysis of extracts (10ug) from 10 Human tissue by using anti-CDH13 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).")
![Immunocytochemistry/Immunofluorescence: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cadherin-13 Antibody (3H6)](http://images.novusbio.com/fullsize/Cadherin-13-Antibody-3H6-Immunocytochemistry-Immunofluorescence-NBP2-02528-img0004.jpg)
![Western Blot: Cadherin-13 Antibody (3H6) [NBP2-02528] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin-13 Antibody (3H6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- Cadherin-13 Antibody (3H6)](http://images.novusbio.com/fullsize/Cadherin-13-Antibody-3H6-Western-Blot-NBP2-02528-img0006.jpg)
![Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815] - Analysis of ICAM5 precursor in paraffin-embedded Human Brain (Cerebral Cortex) using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ICAM-5-Antibody-Immunohistochemistry-Paraffin-NB100-53815-img0003.jpg "Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815] - Analysis of ICAM5 precursor in paraffin-embedded Human Brain (Cerebral Cortex) using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HAP1-Antibody-Western-Blot-NB100-55413-img0001.jpg "Western Blot: HAP1 Antibody [NB100-55413] - Staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: HAP1 Antibody [NB100-55413] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HAP1-Antibody-Immunohistochemistry-Paraffin-NB100-55413-img0002.jpg)
![Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Western-Blot-NBP3-16240-img0002.jpg "Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb (NBP3-16240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Immunocytochemistry-Immunofluorescence-NBP3-16240-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16240_rabbit-s5a-angiocidin-mab-5g1x6-5220251255539.jpg)
![Western Blot: IP6K1 Antibody [NBP3-36710] - Western blot analysis of various lysates using IP6K1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-36710_rabbit-ip6k1-pab-91220241418121.jpg "Western Blot: IP6K1 Antibody [NBP3-36710] - Western blot analysis of various lysates using IP6K1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunohistochemistry: IP6K1 Antibody [NBP3-36710] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using IP6K1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-36710_rabbit-ip6k1-pab-9122024159532.jpg)
![Immunohistochemistry: IP6K1 Antibody [NBP3-36710] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IP6K1 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-36710_rabbit-ip6k1-pab-9122024141813.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0003.jpg "Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of C6 cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 [ac Lys29] Antibody [NBP3-15486] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] HMGB1/HMG-1 Rabbit pAb (NBP3-15486) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-[ac-Lys29]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15486-img0004.jpg)