ICAM-5 Antibody

Immunohistochemistry-Paraffin: ICAM-5 Antibody [NB100-53815] - Analysis of ICAM5 precursor in paraffin-embedded Human Brain (Cerebral Cortex) using this antibody at 2.5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ICAM-5 Antibody Summary

• Description: Novus Biologicals Goat ICAM-5 Antibody (NB100-53815) is a polyclonal antibody validated for use in IHC and ELISA. Anti-ICAM-5 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ESPAEGEVFAIQ corresponding to C-Terminus according to NP_003250.3.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ICAM5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave an approx. 200-250 kDa band in lysates of human brain (frontal cortex and hippocampus) and mouse brain after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2 kDa band according to NP_003250.2. The 200-250 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cerebral cortex) shows axon/dendrite staining leaving cell bodies relatively unstained.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ICAM-5 Antibody

• ICAM5
• ICAM-5
• intercellular adhesion molecule 5
• intercellular adhesion molecule 5, telencephalin
• Telencephalin
• TLCNTelencephalin
• TLNtelencephalin

Background

ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-5 Antibody (NB100-53815)(3)

Publications using NB100-53815

• Apostolo N, Smukowski Sn, Vanderlinden J Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun 2020-10-15 [PMID: 33057002]
• Perez-Straziota C, Gaster RN, Rabinowitz YS. CORNEAL CROSS-LINKING FOR PEDIATRIC KERATCOCONUS REVIEW Cornea 2018-06-01 [PMID: 29601364]
• Yoshihara Y, Oka S, Nemoto Y et al. An ICAM-related neuronal glycoprotein, telencephalin, with brain segment-specific expression. Neuron 1994-03-01 [PMID: 7794412]

Bioinformatics

• Gene Symbol: ICAM5
• Entrez:
  • 7087(Human)
  • 15898(Mouse)
  • 313785(Rat)
• Uniprot:
  • NP_003250.2(Human)