| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-44270-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73587 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50031SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86376-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05551-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50058SS | |||
![Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Western-Blot-NBP1-44270-img0009.jpg "Western Blot: SLC12A3 Antibody [NBP1-44270] - Western blot analysis of Rat tissue lysates showing detection of SLC12A3 protein using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:1000.")
![Immunohistochemistry: SLC12A3 Antibody [NBP1-44270] - Immunohistochemistry analysis using Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-SLC12A3 Polyclonal Antibody (NBP1-44270) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-NBP1-44270-img0010.jpg)
![Western Blot: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D .](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Western-Blot-NBP2-45387-img0005.jpg "Western Blot: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R5D .")
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0004.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0001.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0002.jpg)
![Immunohistochemistry: PPP2R5D Antibody (5E7) [NBP2-45387] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-5E7-Immunohistochemistry-NBP2-45387-img0003.jpg)
![Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-NB100-1935-img0001.jpg "Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.")
![CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.](http://images.novusbio.com/fullsize/CNPase-Antibody-NB100-1935-img0002.jpg)
![Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Western-Blot-NBP2-50031-img0004.jpg "Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (1H10) [NBP2-50031] - Mixed neuron-glial cell cultures stained with NBP2-50031 (red) and polyclonal antibody against GFAP (green, NB300-141). NBP2-50031 stains strongly in oligodendrocytes, whereas GFAP labels only the intermediate filaments in astrocytes. Blue is DNA staining.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Immunocytochemistry-Immunofluorescence-NBP2-50031-img0003.jpg)
![Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031] - Analysis of rat cerebellum section stained with mouse mAb to CNP, NBP2-50031, dilution 1:500 in green and costained with chicken pAb to NF-M, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. NF-M antibody labels axons of neuronal cells.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Immunocytochemistry-Immunofluorescence-NBP2-50031-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Analysis in human kidney and liver tissues using NBP1-86376 antibody. Corresponding SLC12A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Analysis in human kidney and liver tissues using NBP1-86376 antibody. Corresponding SLC12A3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0012.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0006.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0010.jpg)
![Immunohistochemistry-Paraffin: SLC12A3 Antibody [NBP1-86376] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SLC12A3-Antibody-Immunohistochemistry-Paraffin-NBP1-86376-img0011.jpg)
![Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NBP3-05551-img0002.jpg "Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP3-05551] - Immunofluorescent analysis of a section of mouse striatum stained with CNPase Antibody at a dilution of 1:3,000 in green, and costained with rabbit pAb to NF-L at a dilution of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNPase antibody stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The NF-L antibody labels axons of neuronal cells enclosed by the myelin.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05551-img0001.jpg)
![Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NBP2-50058-img0004.jpg "Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50058-img0003.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88960] - Analysis using Anti-PPP2R5D antibody NBP1-88960 (A) shows similar pattern to independent antibody NBP1-88959 (B).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88960-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PPP2R5D Antibody [NBP1-88960] - Analysis using Anti-PPP2R5D antibody NBP1-88960 (A) shows similar pattern to independent antibody NBP1-88959 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PPP2R5D Antibody [NBP1-88960] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Immunohistochemistry-Paraffin-NBP1-88960-img0007.jpg)
![Western Blot: PPP2R5D Antibody [NBP1-88960] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5D-Antibody-Western-Blot-NBP1-88960-img0006.jpg)
![Western Blot: CNPase Antibody [NBP1-85998] - Analysis using Anti-CNP antibody NBP1-85998 (A) shows similar pattern to independent antibody NBP1-85999 (B).](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NBP1-85998-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CNPase Antibody [NBP1-85998] - Analysis using Anti-CNP antibody NBP1-85998 (A) shows similar pattern to independent antibody NBP1-85999 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85998] - Staining in human cerebral cortex and pancreas tissues using anti-CNP antibody. Corresponding CNP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85998-img0007.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85998] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85998-img0005.jpg)
![Immunohistochemistry-Paraffin: CNPase Antibody [NBP1-85998] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Paraffin-NBP1-85998-img0006.jpg)
![Western Blot: CNPase Antibody [NB110-93362] - Rat brain lysate showing the specific immunolabeling of the ~ 46k CNP protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NB110-93362-img0003.jpg "Western Blot: CNPase Antibody [NB110-93362] - Rat brain lysate showing the specific immunolabeling of the ~ 46k CNP protein.")
![Immunohistochemistry-Frozen: CNPase Antibody [NB110-93362] - Staining of saline treated mouse cortex cryosections with CNPase antibody (green). Cell nuclei are visualized with DAPI DNA stain (blue).](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-Frozen-NB110-93362-img0004.jpg)
![Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)](http://images.novusbio.com/fullsize/SLC12A3-[p-Thr53]-Antibody-Western-Blot-NBP2-60775-img0001.jpg "Western Blot: SLC12A3 [p Thr53] Antibody [NBP2-60775] - Western blot of mouse kidney lysate showing specificimmunolabeling of the ~160 kDa NCC protein phosphorylatedat Thr53 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where immunolabeling is completelyeliminated by blot treatment with lambda phosphatase (Lambda-Ptase, 1200 units for 30 min)")
![Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-288202412461614.jpg "Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-298202414304124.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32847, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-392024884664.jpg)