| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05529-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05549-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05528-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05556-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05550-50ul | |||
![Western Blot: beta-Synuclein Antibody [NBP3-05529] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a at a dilution of of 1:1,000 in green: [1] protein standard (red), [2] rat cortex [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 17 kDa corresponds to beta-Synuclein protein.](http://images.novusbio.com/fullsize/beta-Synuclein-Antibody-Western-Blot-NBP3-05529-img0003.jpg "Western Blot: beta-Synuclein Antibody [NBP3-05529] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a at a dilution of of 1:1,000 in green: [1] protein standard (red), [2] rat cortex [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 17 kDa corresponds to beta-Synuclein protein.")
![Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/beta-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05529-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05529] - Immunofluorescent analysis of mouse hippocampus section stained with beta-Synuclein Antibody at a at a dilution of of 1:500 in green, and costained with rabbit polyclonal antibody to C-terminal peptide of rat NF-L at a at a dilution of of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein Antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/beta-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05529-img0001.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0005.jpg "Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (NB100-40828). Detection: DAB](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-NB100-40828-img0006.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Spectrin beta 2 Antibody [NB100-40828] - Detection of human Spectrin beta 2 (SPTBN1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Spectrin beta 2 antibody NB100-40828 used for IP at 3 ug per reaction. SPTBN1 was also immunoprecipitated by rabbit anti-Spectrin beta 2 antibody NB100-40829. For blotting immunoprecipitated SPTBN1, NB100-40828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunoprecipitation-NB100-40828-img0007.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05549-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05549] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody at a dilution of 1:3,000, in green. [1, 6] Protein standard, [2] Rat brain, [3] Cow cortex, [4] HEK293 cells, [5] COS-1 cells, [7] Rat cortex, [8] rat cortex membrane enriched fraction, [9] mouse cortex, [10] mouse cortex membrane enriched fraction. Bands at 60-190kDa represent Ankyrin 3 splice variants and proteolytic fragments. Larger Ankyrin 3 isoforms of 270kDa and 480kDa can be seen on longer exposure of the blots.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05549] - Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with Ankyrin 3 Antibody, CPCA-ANK3, at a dilution of 1:2,000 in green, and costained with mouse mAb to RBFOX3/NeuN at a dilution of 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The Ankyrin 3 antibody stains the axonal initial segments, while the RBFOX3/NeuN antibody reveals perikarya and nuclei of neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05549-img0001.jpg)
![Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Western-Blot-NBP3-05528-img0002.jpg "Western Blot: Ankyrin 3 Antibody [NBP3-05528] - Western blot analysis of different tissue and cell lysates using Ankyrin 3 Antibody, at a at a dilution of of 1:2,000 in green: [1] protein standard (red), [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, [6] SH-SY5Y cells, [7] rat brain, and [8] mouse brain lysates. Bands at ~190kDa represent ankyrin 3 splice variants, higher molecular weight bands at 270kDa and 480kDa can be seen on longer exposure. Lower molecular weight bands are likely proteolytic fragments.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05528] - Immunofluorescent analysis of cortex section of rat brain stained with Ankyrin 3 Antibody at a at a dilution of of 1:2,000 in green and costained with rabbit polyclonal antibody to NF-L at a at a dilution of of 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 Antibody stains the axonal initial segments, while the NF-L antibody labels dendrites and axons of neuronal cells.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05528-img0001.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40829] - Detection of Human SPTBN1 on HeLa whole cell lysate using NB100-40829. SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 antibody NB100-40828.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40829-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Spectrin beta 2 Antibody [NB100-40829] - Detection of Human SPTBN1 on HeLa whole cell lysate using NB100-40829. SPTBN1 was also immunoprecipitated by rabbit anti-SPTBN1 antibody NB100-40828.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NB100-40829] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-Paraffin-NB100-40829-img0005.jpg)
![Western Blot: beta-Synuclein Antibody [NBP3-05556] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] mouse cerebellum [3] mouse hippocampus, [4] rat cerebellum, [5] rat hippocampus, and [6] cow cerebellum. Strong band at about 17kDa corresponds to the beta-Synuclein protein.](http://images.novusbio.com/fullsize/beta-Synuclein-Antibody-Western-Blot-NBP3-05556-img0002.jpg "Western Blot: beta-Synuclein Antibody [NBP3-05556] - Western blot analysis of different tissue lysates using beta-Synuclein Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] mouse cerebellum [3] mouse hippocampus, [4] rat cerebellum, [5] rat hippocampus, and [6] cow cerebellum. Strong band at about 17kDa corresponds to the beta-Synuclein protein.")
![Immunocytochemistry/Immunofluorescence: beta-Synuclein Antibody [NBP3-05556] - Immunofluorescent analysis of rat cerebellum section stained with beta-Synuclein Antibody at a dilution of 1:1,000 in red, and costained with chicken pAb to parvalbumin at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The beta-Synuclein antibody detects protein concentrated in synaptic regions, and parvalbumin antibody labels the perikarya and dendrites of Purkinje cells, and interneurons in the molecular layer of the cerebellum.](http://images.novusbio.com/fullsize/beta-Synuclein-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05556-img0001.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-20871-img0001.jpg "Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: PMP70 Antibody [NBP1-20871] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PMP70-Antibody-Flow-Cytometry-NBP1-20871-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ATG16L1-Antibody-Immunohistochemistry-Paraffin-NBP2-15504-img0002.jpg "Immunohistochemistry-Paraffin: ATG16L1 Antibody [NBP2-15504] - Immunohistochemical analysis of paraffin-embedded U251 xenograft, using antibody at 1:500 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG16L1 Antibody [NBP2-15504] - Wild-type (WT) and ATG16L1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG16L1 antibody [N2C1], Internal diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15504_rabbit-polyclonal-atg16l1-antibody-161020241940578.jpg)
![Immunohistochemistry-Paraffin: MOBP Antibody [NBP1-89430] - Staining of human cerebral cortex shows moderate dot-like positivity.](http://images.novusbio.com/fullsize/MOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-89430-img0008.jpg "Immunohistochemistry-Paraffin: MOBP Antibody [NBP1-89430] - Staining of human cerebral cortex shows moderate dot-like positivity.")
![Immunohistochemistry-Paraffin: MOBP Antibody [NBP1-89430] - Staining of human duodenum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/MOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-89430-img0009.jpg)
![Immunohistochemistry-Paraffin: MOBP Antibody [NBP1-89430] - Staining of human kidney shows no positivity as expected.](http://images.novusbio.com/fullsize/MOBP-Antibody-Immunohistochemistry-Paraffin-NBP1-89430-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0002.jpg "Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Western blot analysis of different tissue lysates using Ankyrin 3 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cortex membrane enriched fraction, [4] mouse cortex and [5] mouse cortex membrane enriched fraction. The band at ~190kDa correspond to one of the three high molecular weight forms of Ankyrin 3, the 270kDa and 480kDa isoforms can be seen on longer exposure of the blot.")
![Immunocytochemistry/Immunofluorescence: Ankyrin 3 Antibody [NBP3-05550] - Immunofluorescent analysis of a rat brain section stained with Ankyrin 3 Antibody at a dilution of 1:1,000, in red and costained with mouse mAb to MAP2 at a dilution of 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The Ankyrin 3 antibody stains axonal initial segments, while the MAP2 antibody labels MAP2 protein expressed in the perikarya and dendrites of most neurons.](http://images.novusbio.com/fullsize/Ankyrin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05550-img0001.jpg)
![Immunoprecipitation: Spectrin beta 2 Antibody - BSA Free [NBP3-45826] - IP Result of anti-Spectrin beta 2 (IP:, 5ug; Detection: 1:600) with HEK-293 cells lysate 1000ug.](http://images.novusbio.com/fullsize/nbp3-45826_rabbit-spectrin-beta-2-pab-bsa-free-284202512593082.jpg "Immunoprecipitation: Spectrin beta 2 Antibody - BSA Free [NBP3-45826] - IP Result of anti-Spectrin beta 2 (IP:, 5ug; Detection: 1:600) with HEK-293 cells lysate 1000ug.")
![Western Blot: Spectrin beta 2 Antibody - BSA Free [NBP3-45826] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (Spectrin beta 2 antibody) at dilution of 1:300](http://images.novusbio.com/fullsize/nbp3-45826_rabbit-spectrin-beta-2-pab-bsa-free-2842025138757.jpg)
![Immunohistochemistry: Spectrin beta 2 Antibody - BSA Free [NBP3-45826] - Immunohistochemistry of paraffin-embedded human lung using (Spectrin beta 2 antibody) at dilution of 1:100](http://images.novusbio.com/fullsize/nbp3-45826_rabbit-spectrin-beta-2-pab-bsa-free-28420251258716.jpg)
![Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-288202412461622.jpg "Western Blot: PMP70 Antibody (JE39-00) [NBP3-32833] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32833) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (JE39-00) [NBP3-32833] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody (NBP3-32833) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32833) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32833_rabbit-pmp70-mab-je39-00-298202414304140.jpg)