| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44011 | |||
| BSA Free | NBP2-52925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44413 | |||
| BSA Free | NBP2-76477-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90283-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86169-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75775 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43791 | |||
| BSA Free | NBP2-46621-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44495 | |||
| BSA Free | NBP2-61632-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74344 | |||
![Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Western-Blot-NBP2-52925-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunocytochemistry-Immunofluorescence-NBP2-52925-img0009.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0006.jpg)
![Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Western-Blot-NBP2-52925-img0007.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0001.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0002.jpg)
![Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-NBP2-52925-img0003.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human pancreas shows absense of staining (negative control).](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0004.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0005.jpg)
![Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GFAP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Western-Blot-NBP2-76477-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GFAP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of human cerebral cortex shows strong cytoplasmic positivity in astrocytes.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0003.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of rat brain shows strong positivity in astrocytes in the cerebellum.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0008.jpg)
![Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Western-Blot-NBP2-76477-img0010.jpg)
![Western Blot: GFAP Antibody (CL2713) [NBP2-76477] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Western-Blot-NBP2-76477-img0002.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0004.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of mouse brain shows strong positivity in astrocytes in the cerebellum.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0005.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of mouse brain shows strong positivity in astrocytes in the brainstem.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0006.jpg)
![Immunohistochemistry-Paraffin: GFAP Antibody (CL2713) [NBP2-76477] - Staining of rat brain shows strong positivity in astrocytes in the hippocampus.](http://images.novusbio.com/fullsize/GFAP-Antibody-CL2713-Immunohistochemistry-Paraffin-NBP2-76477-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90283-img0011.jpg "Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human kidney, liver, pancreas and testis using Anti-FLNA antibody NBP1-90283 (A) shows similar protein distribution across tissues to independent antibody NBP1-90284 (B).](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0013.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis using Anti-FLNA antibody NBP1-90283.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0026.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis shows moderate cytoplasmic positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0018.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0010.jpg "Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0007.jpg)
![Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP1-86169] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SOD2-Mn-SOD-Antibody-Immunohistochemistry-Paraffin-NBP1-86169-img0009.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (rUCHL1/775)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Western-Blot-NBP2-75773-img0008.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (rUCHL1/775)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (rUCHL1/775) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Immunocytochemistry-Immunofluorescence-NBP2-75773-img0006.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Immunohistochemistry-Paraffin-NBP2-75773-img0004.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (rUCHL1/775)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Flow-Cytometry-NBP2-75773-img0005.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Analysis of Human Brain tissue lysate using Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Western-Blot-NBP2-75773-img0003.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Formalin-fixed, paraffin-embedded Human Brain stained with Pgp9.5 Mouse Recombinant Monoclonal Antibody (rUCHL1/775).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Immunohistochemistry-Paraffin-NBP2-75773-img0001.jpg)
![Protein Array: UCH-L1/PGP9.5 Antibody (rUCHL1/775) [NBP2-75773] - Analysis of Protein Array containing >19,000 full-length human proteins using UCH-L1/PGP9.5 Antibody (rUCHL1/775) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-rUCHL1-775-Protein-Array-NBP2-75773-img0007.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0008.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Staining of U-251 cells using the Anti-UCHL1 monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunocytochemistry-Immunofluorescence-NBP2-46621-img0010.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human fallopian tube shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0006.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251 AAb](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0007.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Western-Blot-NBP2-46621-img0009.jpg)
![Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of rat cerebral cortex shows moderate immunoreactivity in neurons.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0001.jpg)
![Immunohistochemistry: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of mouse trigeminal ganglion shows strong immunoreactivity in primary sensory neurons.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0002.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human cerebellum shows moderate positivity in Purkinje cells.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0003.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human prostate shows staining in peripheral nerve fibers.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0004.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (CL3210) [NBP2-46621] - Analysis of human duodenum shows staining in peripheral nerve fibers.](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-CL3210-Immunohistochemistry-NBP2-46621-img0005.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining in human heart muscle and skin tissues. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of U-251 cells using the Anti-CDH2 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunocytochemistry-Immunofluorescence-NBP2-61632-img0010.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0003.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0004.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0005.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human skin shows no positivity in epidermal cells as expected.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89682-img0013.jpg "Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89682_rabbit-polyclonal-ng2-mcsp-antibody-31020241539744.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human placenta shows moderate membranous and cytoplasmic positivity in trophoblast.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0005.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0006.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0007.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0008.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0013.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunocytochemistry-Immunofluorescence-NBP2-03256-img0012.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0011.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0002.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0003.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0004.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0005.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0006.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0007.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0008.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0009.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0010.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0001.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0002.jpg "Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74344-img0005.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0012.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0004.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0001.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0006.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0007.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0008.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0009.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0010.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0011.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0013.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)