| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44244 | |||
| BSA Free | NBP2-61431-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43957 | |||
| BSA Free | NBP2-59778-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88204-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89682-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90283-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91790-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44011 | |||
| BSA Free | NBP2-52925-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44243 | |||
| BSA Free | NBP2-61430-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44495 | |||
| BSA Free | NBP2-61632-25ul | |||
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of A549 cells. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Western-Blot-NBP2-61431-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunocytochemistry-Immunofluorescence-NBP2-61431-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human cerebellum shows strong positivity in both neuronal cell bodies and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5814) [NBP2-61431] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5814-Immunohistochemistry-Paraffin-NBP2-61431-img0007.jpg)
![Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human testis shows weak immunoreactivity in basal cells of the seminiferous ducts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0005.jpg)
![Western Blot: Profilin 1 Antibody (5216) [NBP2-59778] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Western-Blot-NBP2-59778-img0006.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human fallopian tube shows cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0001.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human tonsil shows moderate to strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0002.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human liver shows strong immunoreactivity in circulating lymphoid cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human kidney shows strong membranous combined with moderate cytoplasmic immunoreactivity in renal tubules.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Lysates of HAP1 WT and PFN1 KO were prepared, and 50 ug of protein were processed for immunoblot with Profilin 1 antibody (NBP2-59778). The Ponceau stained transfer of the blot is shown. Antibody dilution used: NBP2-59778 at 1/1000. Predicted band size: 15 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-CL3524-Knockout-Validated-NBP2-59778-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88204-img0007.jpg "Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0008.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0014.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0011.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0012.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0013.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88204_rabbit-polyclonal-calpain-2-antibody-652025162443.jpg)
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89682-img0013.jpg "Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody [NBP1-89682] - Immunofluorescence (IF) analysis of NG2/CSPG4 expression in glioblastoma (GB)-derived human cell lines. IF of a GB-derived neurosphere cell line for (A) NG2/CSPG4 (green), original magnification (OM) ×200; (B) Nestin (red), OM ×200; (C) MSel1 (red), OM ×200; (D) Notch-2 (green), OM ×200. IF staining of an adherent cell line for (E) NG2/CSPG4 (green), OM ×400; (F) GFAP (green), OM ×400; (G) beta -III Tubulin (red), OM ×200; (H) Galactocerebroside C (red), OM ×200. Nuclei were counterstained with DAPI (blue). DAPI, 4′,6-diamidino-2-phenylindole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32599896), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89682_rabbit-polyclonal-ng2-mcsp-antibody-31020241539744.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human placenta shows moderate membranous and cytoplasmic positivity in trophoblast.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0005.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human prostate shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0006.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0007.jpg)
![Immunohistochemistry-Paraffin: NG2/MCSP Antibody [NBP1-89682] - Immunohistochemical staining of human duodenum shows moderate membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NG2-MCSP-Antibody-Immunohistochemistry-Paraffin-NBP1-89682-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90283-img0011.jpg "Immunocytochemistry/Immunofluorescence: Filamin A Antibody [NBP1-90283] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human kidney, liver, pancreas and testis using Anti-FLNA antibody NBP1-90283 (A) shows similar protein distribution across tissues to independent antibody NBP1-90284 (B).](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0013.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis using Anti-FLNA antibody NBP1-90283.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0026.jpg)
![Immunohistochemistry-Paraffin: Filamin A Antibody [NBP1-90283] - Staining of human testis shows moderate cytoplasmic positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-Immunohistochemistry-Paraffin-NBP1-90283-img0018.jpg)
![Immunohistochemistry-Paraffin: CLCN3 Antibody [NBP1-91790] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/CLCN3-Antibody-Immunohistochemistry-Paraffin-NBP1-91790-img0002.jpg "Immunohistochemistry-Paraffin: CLCN3 Antibody [NBP1-91790] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.")
![Western Blot: CLCN3 Antibody [NBP1-91790] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500](http://images.novusbio.com/fullsize/CLCN3-Antibody-Western-Blot-NBP1-91790-img0005.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp1-91790_rabbit-polyclonal-clcn3-antibody-295202516272519.jpg)
![Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Western-Blot-NBP2-52925-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunocytochemistry-Immunofluorescence-NBP2-52925-img0009.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0006.jpg)
![Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Western-Blot-NBP2-52925-img0007.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0001.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0002.jpg)
![Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-NBP2-52925-img0003.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human pancreas shows absense of staining (negative control).](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0004.jpg)
![Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.](http://images.novusbio.com/fullsize/MEF2C-Antibody-CL0368-Immunohistochemistry-Paraffin-NBP2-52925-img0005.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0010.jpg "Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cerebral cortex tissue.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of A549 cells showing specific staining on microtubules in green. Nuclear probes are visualized in blue. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0012.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0008.jpg)
![Western Blot: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Western-Blot-NBP2-61430-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of SH-SY5Y cells using the Anti-TUBB3 monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunocytochemistry-Immunofluorescence-NBP2-61430-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human cerebellum shows strong positivity in both neurons and neuropil.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human prostate shows strong positivity in the peripheral nerves.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (CL5813) [NBP2-61430] - Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-CL5813-Immunohistochemistry-Paraffin-NBP2-61430-img0007.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CDH2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining in human heart muscle and skin tissues. Corresponding CDH2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of U-251 cells using the Anti-CDH2 monoclonal antibody, showing specific staining in the plasma membrane and cell junctions in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunocytochemistry-Immunofluorescence-NBP2-61632-img0010.jpg)
![Western Blot: N-Cadherin Antibody (CL3716) [NBP2-61632] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Western-Blot-NBP2-61632-img0009.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human heart muscle shows strong positivity in the intercalated discs of cardiomyocytes.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0002.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0003.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0004.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human liver cancer (hepatocellular carcinoma) shows moderate to strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0005.jpg)
![Immunohistochemistry-Paraffin: N-Cadherin Antibody (CL3716) [NBP2-61632] - Staining of human skin shows no positivity in epidermal cells as expected.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-CL3716-Immunohistochemistry-Paraffin-NBP2-61632-img0006.jpg)
![Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Western-Blot-NBP2-37816-img0014.jpg "Western Blot: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of 25 ug of various brain, SHSY5Y, and HeLa cell lysates and 10ul of PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin in SHSY5Y cells (human neuroblast) either left untreated (left panel) or treated with 10uM retinoic acid (right panel) for 72 hours. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a beta-3 Tubulin monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37816-img0011.jpg)
![Immunohistochemistry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Normal biopsies of deparaffinized human brain tissues.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunohistochemistry-NBP2-37816-img0012.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin (blue histogram) on human SHSY5Y neuroblast cells. Cells were harvested, fixed with 4% formaldehyde, washed with PBS, and incubated with a beta-3 Tubulin monoclonal antibody at a 1:50 dilution or PBS alone (red histogram) for 1 hour at room temperature. For flow cytometry analysis, a 30-minute incubation with DyLight 488 goat anti-mouse IgG secondary antibody was performed and 10,000 cells were acquired for each sample.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Flow-Cytometry-NBP2-37816-img0010.jpg)
![Immunoprecipitation: beta-III Tubulin Antibody (2G10) [NBP2-37816] - Analysis of beta-3 Tubulin was performed using SHSY5Y whole cell lysate. Antigen-antibody complexes were formed by incubating 300ug of lysate with 5ug of a beta-3 Tubulin monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. SHSY5Y cell lysate (30ug) was loaded as a positive control (left lane). The sample was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a beta-3 Tubulin monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-2G10-Immunoprecipitation-NBP2-37816-img0013.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-Knockout-Validated-NBP2-24533-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP2-24533) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody [NBP2-24533] - Analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1:5000 and 2) peptide affinity purified HSP27 antibody at 2.0 ug/mL. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Western-Blot-NBP2-24533-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NBP2-24533] - FFPE human small intestine probed with HSP27 antibody at 10 ug/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Hsp27-Antibody-Immunohistochemistry-Paraffin-NBP2-24533-img0002.jpg)