| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05215-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91271SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47834-0.1mg | |||
| IHC-Prediluted | NBP2-48252 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84883-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-48318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
![Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0006.jpg "Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NBP1-05215] - Shows mixed neuron/glial rat brain cultures stained with NBP1-05215 (green) and chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) NBP1-05198 (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05215-img0004.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody [NBP1-05215] - Analysis of a mouse hippocampus section stained with NF-H antibody, dilution 1:2,000 (Red), and costained with mouse myelin basic protein mAb (MBP), dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Free-Floating-NBP1-05215-img0007.jpg)
![Western Blot: NF-H Antibody [NBP1-05215] - Blot of rat spinal cord extract blotted with NBP1-05215. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated dendritic and perilaryal form of this protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP1-91271] - Zfp521 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91271-img0003.jpg "Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP1-91271] - Zfp521 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: ZNF521 Antibody [NBP1-91271] - IHC analysis of ZNF521 in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-NBP1-91271-img0004.jpg)
![Western Blot: NF-H Antibody (NE14) [NBP2-44728] - Western Blot Analysis of human Brain tissue lysate using NF-H Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Western-Blot-NBP2-44728-img0005.jpg "Western Blot: NF-H Antibody (NE14) [NBP2-44728] - Western Blot Analysis of human Brain tissue lysate using NF-H Antibody (NE14).")
![Immunohistochemistry-Paraffin: NF-H Antibody (NE14) [NBP2-44728] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Immunohistochemistry-Paraffin-NBP2-44728-img0002.jpg)
![Flow Cytometry: NF-H Antibody (NE14) [NBP2-44728] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NE14).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Flow-Cytometry-NBP2-44728-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NE14) [NBP2-44728] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Immunohistochemistry-Paraffin-NBP2-44728-img0001.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Non-transfected (-) and transfected (+) U87-MG whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0004.jpg)
![Western Blot: Huntingtin Antibody [NBP3-13106] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Huntingtin Antibody [NBP3-13106] - Wild-type (WT) and HTT knockout (KO) 293T cell extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13106_rabbit-huntingtin-pab-161020241940576.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human colon, kidney, testis and vagina using Anti-ZNF521 antibody NBP1-84883 (A) shows similar protein distribution across tissues to independent antibody NBP1-84882 (B).](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84883-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human colon, kidney, testis and vagina using Anti-ZNF521 antibody NBP1-84883 (A) shows similar protein distribution across tissues to independent antibody NBP1-84882 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP1-84883] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84883-img0007.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84883-img0009.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84883-img0011.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human colon.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84883-img0014.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Analysis in human placenta and liver tissues using antibody. Corresponding ZNF521 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-84883_rabbit-polyclonal-znf521-antibody-immunohistochemistry-paraffin-9102024115619.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13158-img0005.jpg "Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunohistochemistry-Frozen-NBP3-13158-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody [NBP3-13158] - NMDAR1 antibody detects NMDAR1 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR1 protein stained by NMDAR1 antibody (NBP3-13158) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13158-img0003.jpg)
![Immunohistochemistry-Paraffin: NMDAR1 Antibody [NBP3-13158] - Rat brain. NMDAR1 antibody (NBP3-13158) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/NMDAR1-Antibody-Immunohistochemistry-Paraffin-NBP3-13158-img0001.jpg)
![Western Blot: NMDAR1 Antibody [NBP3-13158] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NMDAR1 antibody (NBP3-13158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13158_rabbit-nmdar1-pab-91020242041289.jpg)
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024936274.jpg "Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422681.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425469.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425455.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422643.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024940886.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0001.jpg "USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE prostate carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.")
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of mouse USP7/HAUSP in FFPE renal cell carcinoma by IHC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0002.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP in FFPE OVCAR-4 cells by ICC using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB100-2332). Substrate: DAB.](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0003.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human and mouse USP7/HAUSP by WB of RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 lysate using this antibody. Secondary: HRP-conjugated goat anti-rabbit IgG (NB7160). Lower Panel: Rabbit anti-eEF2 (NB100-87020).](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0004.jpg)
![USP7 Antibody (BLR072G) [NBP2-78709] - Detection of human USP7/HAUSP by WB of immunoprecipitates from HEK293T lysate using this antibody and rabbit anti-USP7 (NB100-2296).](http://images.novusbio.com/fullsize/USP7-Antibody-BLR072G-NBP2-78709-img0005.jpg)
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Rab7a-Antibody-4X3P6-Western-Blot-NBP3-15444-img0002.jpg "Western Blot: Rab7a Antibody (4X3P6) [NBP3-15444] - Western blot analysis of extracts of various cell lines, using Rab7a Rabbit mAb (NBP3-15444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Rab7a Antibody (5A10A4) [Rab7a] - Western blot analysis of various lysates using Rab7a Rabbit mAb at 1:55000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-15444_rabbit-rab7a-mab-5a10a4-52202517152713.jpg)
![Immunocytochemistry/ Immunofluorescence: Rab7a Antibody (5A10A4) [Rab7a] - Confocal imaging of NIH/3T3 cells using Rab7a Rabbit mAb followed by a further incubation with ABflo 488-conjugated Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with Cy3 Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15444_rabbit-rab7a-mab-5a10a4-52202517213545.jpg)
![Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Western-Blot-NBP1-48318-img0005.jpg "Western Blot: TRP7 Antibody (N64A/36) [NBP1-48318] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpC7 protein using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0007.jpg)
![Immunohistochemistry: TRP7 Antibody (N64A/36) [NBP1-48318] - Immunohistochemistry analysis using Mouse Anti-TrpC7 Monoclonal Antibody, Clone N64A/36 (NBP1-48318). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody (NBP1-48318) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-NBP1-48318-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: TRP7 Antibody (N64A/36) [NBP1-48318] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpC7 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear staining .](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunocytochemistry-Immunofluorescence-NBP1-48318-img0004.jpg)
![Immunohistochemistry-Paraffin: TRP7 Antibody (N64A/36) [NBP1-48318] - Mouse backskin sections. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/TRP7-Antibody-N64A-36-Immunohistochemistry-Paraffin-NBP1-48318-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)