| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48226 | |||
| Azide and BSA Free | NBP2-34705-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72642 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88661-25ul | |||
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0023.jpg "Western Blot: Pax6 Antibody [NB300-750] - Analysis of sheep retinal extract")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - ARPE-19 monolayer on glass substrate. Fixative Methanol Blocking Goat Serum. Dilution 1:100. Incubation 1h @ RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-NB300-750-img0024.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of rat whole eye extract](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0017.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of various human, mouse and non-human primate whole cell lysates.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0016.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of extracts from HeLa, U251 and mouse brain using PAX6 antibody (NB300-750, 1:100).](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0019.jpg)
![Western Blot: Pax6 Antibody [NB300-750] - Analysis of Pax6 in skate (Leucoraja erinacea) brain lysate using anti-Pax6 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB300-750-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NB300-750] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NB300-750-img0011.jpg)
![Immunoprecipitation: Pax6 Antibody [NB300-750] - Analysis of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500ug whole cell lysate with 5ug of PAX6 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunoprecipitation-NB300-750-img0020.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NB300-750] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs).(a) Schematic description of the two-stage protocol applied to iPSC-renal commitment. (b, c, d, e) Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. (b) The pluripotency markers SSEA4, TRA-1-81, Nanog, ME marker T(Bry) & IM marker Osr1. (c) IM & MM marker expression as Wt1, Pax8, Pax2, Six2 & Sall1. (d) Renal progenitor markers CD133, CD24, NCAM, glomerular epithelial marker Claudin1 & proximal tubular epithelial markers AQP1, GGT1. (e) Markers identifying endodermal AFP, ectodermal Pax6, & cardiac mesodermal Nkx2.5 derivation. Nuclei are stained with DAPI (blue). Scale bars: 20 μm (b, c, d, e). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep08826), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-750_rabbit-polyclonal-pax6-antibody-3102024161898.jpg)
![Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Frozen-NBP2-19711-img0010.jpg "Immunohistochemistry-Frozen: Pax6 Antibody [NBP2-19711] - Frozen sectioned E13.5 Rat brain. Green: PAX6 protein stained by PAX6 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunoprecipitation: PAX6 Antibody [NBP2-19711] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 ug of PAX6 antibody 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody diluted at 1:1000. anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/PAX6-Antibody-Immunoprecipitation-NBP2-19711-img0004.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420104458.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202419593540.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-910202420115034.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242075957.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-91020242001050.jpg)
![PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-immunohistochemistry-paraffin-26112025196529.jpg)
![PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19711_rabbit-polyclonal-pax6-antibody-immunohistochemistry-paraffin-26112025199112.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16679_rabbit-polyclonal-glutamate-dehydrogenase-antibody-immunohistochemistry-paraffin-261120251981014.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP2-16679] - Metabolic alterations in the MPC1-/- cells expressed significantly higher levels glutaminolysis related proteins GDH (Glutamate Dehydrogenase) and GLS (NBP2-29940), but there was no significant change in the PDHE1 protein expression in these cells as shown in representative western blots. The alpha-Tublin was used as loading control. ***PSample: Paraffin-embedded rat liver.Glutamate Dehydrogenase stained by GLUD1 + GLUD2 antibody (NBP2-16679) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0003.jpg "Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0004.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in MOLT-4 cell lysates in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP2-24691-img0002.jpg)
![Immunohistochemistry-Paraffin: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in FFPE mouse pancreas tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunohistochemistry-Paraffin-NBP2-24691-img0001.jpg)
![Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.](http://images.novusbio.com/fullsize/Pax6-Antibody-PAX6-498-Western-Blot-NBP2-32893-img0001.jpg "Western Blot: Pax6 Antibody (PAX6/498) [NBP2-32893] - Analysis of PAX6 in human cerebellum lysate.")
![Western Blot: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis homeobox 3.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Western-Blot-NBP2-03933-img0015.jpg "Western Blot: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis homeobox 3.")
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human tonsil using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0014.jpg)
![Flow Cytometry: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Meis homeobox 3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Flow-Cytometry-NBP2-03933-img0016.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0009.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0010.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human Kidney tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0011.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human pancreas tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0012.jpg)
![Immunohistochemistry-Paraffin: Meis homeobox 3 Antibody (3E12) [NBP2-03933] - Staining of paraffin-embedded Human prostate tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-3E12-Immunohistochemistry-Paraffin-NBP2-03933-img0013.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0005.jpg "Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunofluorescence analysis of NIH-3T3 cells using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Immunocytochemistry-Immunofluorescence-NBP3-16582-img0004.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Analysis of extracts of various cell lines, using Glutamate Dehydrogenase Rabbit mAb (NBP3-16582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-7L7N2-Western-Blot-NBP3-16582-img0006.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511383740.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-522025125432.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361270.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411443.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-5220251215457.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511361223.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511411490.jpg)
![Immunohistochemistry: Glutamate Dehydrogenase Antibody (7L7N2) [NBP3-16582] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Glutamate Dehydrogenase Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16582_rabbit-glutamate-dehydrogenase-mab-7l7n2-52202511365186.jpg)
![Western Blot: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis h](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Western-Blot-NBP2-72642-img0001.jpg "Western Blot: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Meis homeobox 3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Meis h")
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Human prostate tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0007.jpg)
![Flow Cytometry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Meis homeobox 3 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72642-img0002.jpg)
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0003.jpg)
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0004.jpg)
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Human Kidney tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0005.jpg)
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Human pancreas tissue using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0006.jpg)
![Immunohistochemistry: Meis homeobox 3 Antibody (OTI3E12) - Azide and BSA Free [NBP2-72642] - Staining of paraffin-embedded Human tonsil using anti-Meis homeobox 3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Meis-homeobox-3-Antibody-OTI3E12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72642-img0008.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0011.jpg "Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of NEUROD1 expression in transfected 293T cell line by NEUROD1 monoclonal antibody (M01), clone 3H8.Lane 1: NEUROD1 transfected lysate(39.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00004760-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: NeuroD1 Antibody (3H8) [H00004760-M01] - Analysis of monoclonal antibody to NEUROD1 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Immunohistochemistry-Paraffin-H00004760-M01-img0009.jpg)
![Western Blot: NeuroD1 Antibody (3H8) [H00004760-M01] - Western Blot analysis of NEUROD1 expression in IMR-32 ( Cat # L008V1 ).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-Western-Blot-H00004760-M01-img0001.jpg)
![ELISA: NeuroD1 Antibody (3H8) [H00004760-M01] - Detection limit for recombinant GST tagged NEUROD1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NeuroD1-Antibody-3H8-ELISA-H00004760-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89100-img0007.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody [NBP1-89100] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human endometrium shows no positivity as expected.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0012.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human pancreas shows strong nuclear positivity in islet cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human cerebellum shows moderate to strong nuclear positivity in cells in granular layer](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0009.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody [NBP1-89100] - Staining of human stomach shows strong nuclear positivity in a subset glandular cells.](http://images.novusbio.com/fullsize/Pax6-Antibody-Immunohistochemistry-Paraffin-NBP1-89100-img0011.jpg)
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/NeuroD1-Antibody-Western-Blot-NBP1-88661-img0006.jpg "Western Blot: NeuroD1 Antibody [NBP1-88661] - Analysis in control (vector only transfected HEK293T lysate) and NEUROD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of the four key molecules by WB in SCLC cell lines. NeuroD1 was strongly expressed in H82 cells. Beta-actin served as an internal control. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-20920231412573.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-2092023181150.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - IHC of the co-expression of key molecules other than ASCL1 in SCLC samples. Serial section immunostaining for NEUROD1 (A) & YAP1 (B): SCLC cell nuclei were positive for NEUROD1 (A), & the cytoplasm was positive for YAP1 (B). Serial section immunostaining for NEUROD1 (C) & POU2F31 (D): SCLC cell nuclei were positive for NEUROD1 (C), & the cytoplasm was positive for POU2F3 (D). The cytoplasm of SCLC cells was positive for YAP1 (E) & POU2F3 (F). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202415395953.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - IHC of the co-expression of key molecules other than ASCL1 in SCLC samples. Serial section immunostaining for NEUROD1 (A) & YAP1 (B): SCLC cell nuclei were positive for NEUROD1 (A), & the cytoplasm was positive for YAP1 (B). Serial section immunostaining for NEUROD1 (C) & POU2F31 (D): SCLC cell nuclei were positive for NEUROD1 (C), & the cytoplasm was positive for POU2F3 (D). The cytoplasm of SCLC cells was positive for YAP1 (E) & POU2F3 (F). Scale bar = 100 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-31020241534377.jpg)
![Immunohistochemistry: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of four key molecules by WB & IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells & weakly expressed in H82 & SBC3 cells. YAP1 was strongly expressed in SBC3 cells. beta -actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, & SBC3 cells. The Pou2F3 staining intensity was weak in H82 & SBC3 cells & strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202416114786.jpg)
![Western Blot: NeuroD1 Antibody [NBP1-88661] - Example of the positive control of four key molecules by WB & IHC in SCLC samples. (A) Example of the positive control of the four key molecules by WB in SCLC cell lines. ASCL1 was strongly expressed in H209 cells. NeuroD1 was strongly expressed in H82 cells. Pou2F3 was strongly expressed in H526 cells & weakly expressed in H82 & SBC3 cells. YAP1 was strongly expressed in SBC3 cells. beta -actin served as an internal control. (B) Example of the positive control of the four key molecules in xenotransplanted tumor tissues from the four cell lines in mice by IHC. ASCL1 staining was found in tumor cell nuclei of H209 cells. NeuroD1 staining was found in the tumor cell nuclei of H82 cells. Pou2F3 staining with a diffuse cytoplasmic pattern was found in H82, H526, & SBC3 cells. The Pou2F3 staining intensity was weak in H82 & SBC3 cells & strong in H526 cells. YAP1 was stained with a membranous pattern in SBC3 cells. Scale bar = 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33202998), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88661_rabbit-polyclonal-neurod1-antibody-310202416114787.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - The results of western blotting and RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1alpha, GLS1 and GLUD1 (Glutamate Dehydrogenase) is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - The results of western blotting and RT-PCR assayA. shows Western blotting results, where the PDHA1 protein expression in the parental LNCaP cells is strongly positive, but its expression in the PDHA1 KO cells is almost negative with two different PDHA1 antibodies. Comparatively, the protein expression of the MPC1 and MPC2 is comparatively decreased and the protein expression of the GLUT1, HK2, LDHA, HIF1alpha, GLS1 and GLUD1 (Glutamate Dehydrogenase) is increased in the LNCaP PDHA1 KO cells. GAPDH was added as a loading control. Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Glutamate Dehydrogenase Antibody [NBP1-54961] - GLS1 and GLUD1 (Glutamate Dehydrogenase) are over-expressed in prostate cancers and associated with poor survival. Immunohistochemical staining of GLUD1 in human prostate cancers with Gleason score 4 (D), 7 (E). and 9 (F). Image collected and cropped by CiteAb from the following publication (www.oncotarget.com/lookup/doi/10.18632/oncotarget.10782) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-NBP1-54961-img0006.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - HT1080 cell lysate, concentration 1ug/ml.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0001.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - lanes 5: rat kidney cordex lanes 6: rat kidney proximal tubules prepped from cortex lanes 7: LLCPK-F+ pig kidney proximal tubule tissue culture lysate lanes 8: rat brain supernatant](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-54961-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human colon tissue.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human kidney, renal tubular epithelium in cortex.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-54961] - Human pancreas tissue](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-54961-img0005.jpg)