| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-266SS | |||
| Azide and BSA Free | NBP2-80869 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83349-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47440-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04383-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38300-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45337 | |||
| Azide and BSA Free | NBP2-34716-0.1mg | |||
![Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-NB300-266-img0020.jpg "Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.")
![Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Western-Blot-NB300-266-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0015.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-Paraffin-NB300-266-img0013.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0006.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0017.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0016.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth and vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun-eosin (median panel) or with nestin antibody (upper and lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, and each surface was measured in mm^2. The red rectangles indicate the zone of the tumour shown in median (hemalun-eosin staining) and lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median and lower panel 100 um. The graph shows the tumour size in mm^2Â (expressed as the mean +/- SEM of 4 mice). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-20920231412574.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth & vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. (A) Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun–eosin (median panel) or with nestin antibody (upper & lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, & each surface was measured in mm2. The red rectangles indicate the zone of the tumour shown in median (hemalun–eosin staining) & lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median & lower panel 100 µm. The graph shows the tumour size in mm2 (expressed as the mean ± SEM of 4 mice). (B) Representative immunohistochemistry photographs of CD31+ vessels in brain tumour areas of 3 different mice treated with regorafenib (right panel) or without regorafenib (control, left panel). Arrows indicate CD31+ blood vessels. Scale bars, 20 µm. The graph shows the number of CD31+ vessels in tumours per mm2 (expressed as mean ± SEM of 4 mice). * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-310202416212379.jpg)
![Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Western-Blot-NBP1-92717-img0006.jpg "Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0005.jpg)
![Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunohistochemistry-NBP1-92717-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for Vimentin and so appear red. Others contain Vimentin and not Nestin and so appear green - these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0004.jpg)
![Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-83349-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-83349] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83349-img0007.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-47477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47477-img0004.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0010.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0011.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human cerebral cortex, colon, kidney and testis using Anti-AKAP8L antibody NBP2-47440 (A) shows similar protein distribution across tissues to independent antibody NBP2-47441 (B).](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0008.jpg)
![Western Blot: HA95/AKAP8L Antibody [NBP2-47440] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Western-Blot-NBP2-47440-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HA95/AKAP8L Antibody [NBP2-47440] - Immunofluorescent staining of human cell line MCF7 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47440-img0003.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0010.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0004.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0005.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human colon.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0009.jpg)
![Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human kidney.](http://images.novusbio.com/fullsize/HA95-AKAP8L-Antibody-Immunohistochemistry-Paraffin-NBP2-47440-img0011.jpg)
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0006.jpg "Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0005.jpg)
![Western Blot: SUMO1 Antibody (6F8B6) [NBP3-15676] - Western blot analysis of extracts of various cell lines, using SUMO1 antibody (NBP3-15676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/SUMO1-Antibody-6F8B6-Western-Blot-NBP3-15676-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: SUMO1 Antibody (6F8B6) [SUMO1] - Confocal imaging of NIH/3T3 cells using [KO Validated] SUMO1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251733572.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251734543.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251734513.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-52202517315566.jpg)
![Immunohistochemistry: SUMO1 Antibody (6F8B6) [SUMO1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KO Validated] SUMO1 Rabbit mAb at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-5220251733570.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SUMO1 Antibody (6F8B6) [SUMO1] - Western blot analysis of lysates from wild type(WT) and SUMO1 knockout (KO) 293T cells, using [KO Validated] SUMO1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15676_rabbit-sumo1-mab-6f8b6-52202517361210.jpg)
![Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody - BSA Free [LRPPRC] - Immunofluorescence analysis of A-549 cells using LRPPRC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-52202516334136.jpg "Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody - BSA Free [LRPPRC] - Immunofluorescence analysis of A-549 cells using LRPPRC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody - BSA Free [LRPPRC] - Immunofluorescence analysis of HepG2 cells using LRPPRC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-52202516334116.jpg)
![Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody - BSA Free [LRPPRC] - Immunofluorescence analysis of NIH/3T3 cells using LRPPRC Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-52202516283275.jpg)
![Immunohistochemistry: LRPPRC Antibody - BSA Free [LRPPRC] - Immunohistochemistry analysis of paraffin-embedded human breast cancer using LRPPRC Rabbit pAb at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-5220251640296.jpg)
![Immunohistochemistry: LRPPRC Antibody - BSA Free [LRPPRC] - Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LRPPRC Rabbit pAb at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-52202516334121.jpg)
![Western Blot: LRPPRC Antibody - BSA Free [LRPPRC] - Western blot analysis of various lysates, using LRPPRC Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-5220251626464.jpg)
![Western Blot: LRPPRC Antibody - BSA Free [LRPPRC] - Western blot analysis of various lysates, using LRPPRC Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-5220251628016.jpg)
![Immunocytochemistry/ Immunofluorescence: LRPPRC Antibody - BSA Free [LRPPRC] - Confocal immunofluorescence analysis of Hela cells using LRPPRC Rabbit pAb at dilution of 1:400. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04383_rabbit-polyclonal-lrpprc-antibody-5220251640276.jpg)
![HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (NBP2-19767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19767_rabbit-polyclonal-serpin-f1-pedf-antibody-western-blot-261120251839577.jpg "HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PEDF antibody [N1C2] (NBP2-19767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CLE7-homolog-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84498-img0008.jpg "Immunocytochemistry/Immunofluorescence: CLE7 homolog Antibody [NBP1-84498] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum, colon, kidney and liver using Anti-LRPPRC antibody NBP2-38300 (A) shows similar protein distribution across tissues to independent antibody NBP1-83349 (B).](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum, colon, kidney and liver using Anti-LRPPRC antibody NBP2-38300 (A) shows similar protein distribution across tissues to independent antibody NBP1-83349 (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: LRPPRC Antibody [NBP2-38300] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP2-38300-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP2-38300] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38300-img0004.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human liver.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0011.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human colon.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0009.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human cerebellum.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0010.jpg)
![Immunohistochemistry-Paraffin: LRPPRC Antibody [NBP2-38300] - Staining of human kidney.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunohistochemistry-Paraffin-NBP2-38300-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Western-Blot-NBP2-32901-img0004.jpg "Western Blot: SUMO1 Antibody (SM1/495) [NBP2-32901] - Analysis using the Azide and BSA Free version of NBP2-32901. Detection of SUMO1 in human HeLa Cell lysate.")
![Immunocytochemistry/Immunofluorescence: SUMO1 Antibody (SM1/495) [NBP2-32901] - Paraformaldehyde-fixed HepG2 cells stained with SUMO1 Antibody (SM1/495)followed by goat anti-Mouse IgG-CF488 (Green). Cell membranes are stained with Phalloidin (red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunocytochemistry-Immunofluorescence-NBP2-32901-img0007.jpg)
![Immunohistochemistry-Paraffin: SUMO1 Antibody (SM1/495) [NBP2-32901] - Formalin-fixed, paraffin-embedded human tonsil stained with SUMO-1 MAb (SM1/495)](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Immunohistochemistry-Paraffin-NBP2-32901-img0001.jpg)
![Flow Cytometry: SUMO1 Antibody (SM1/495) [NBP2-32901] - Flow Cytometric Analysis of paraformaldehyde-fixed HepG2 cells using SUMO1 Antibody (SM1/495) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/SUMO1-Antibody-SM1-495-Flow-Cytometry-NBP2-32901-img0006.jpg)