| Formulation | Catalog # | Availability | Price | |
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| BSA Free | BC100-494SS | |||
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| BSA Free | NB600-101SS | |||
| Formulation | Catalog # | Availability | Price | |
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| BSA Free | NBP1-47518SS | |||
| Azide and BSA Free | NBP2-80610 | |||
| Formulation | Catalog # | Availability | Price | |
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| BSA Free | NB600-103SS | |||
| Formulation | Catalog # | Availability | Price | |
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| BSA Free | NB100-493SS | |||
| Formulation | Catalog # | Availability | Price | |
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| BSA Free | NBP1-49678SS | |||
| Formulation | Catalog # | Availability | Price | |
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| Azide and BSA Free | NBP2-73645 | |||
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - PPM1H does not dephosphorylate Ser111 of Rab8A or key phosphorylation sites of AMPK & Akt signaling pathways.(A) HEK293 cells were transiently transfected with constructs expressing the indicated components. 24 hr post-transfection, cells were treated ±10 µM CCCP (Carbonyl cyanide m-chlorophenyl hydrazine) for 3 hr to induce activation of the PINK1 kinase & trigger Rab8A phosphorylation at Ser111 (Lai et al., 2015). (B) As in (A) except cells were immunoblotted with the indicated antibodies that recognize key phosphorylation sites of AMPK & Akt signaling pathways. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31663853), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-31020241534312.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - PINK1 & Parkin are phosphorylated after a decrease in delta Ψm in mouse primary neurons. Neurons were infected with lentivirus encoding PINK1-Flag (A), wild-type HA-Parkin (B) or HA-Parkin with either the S65A or S65E mutation (C). Cells were treated with the mitochondrial uncoupler CCCP (30 μm) for 1–3 h & subjected to SDS-PAGE in the absence or presence of 50 μm phos-tag. Note that mobility does not reflect the molecular weight of proteins in phos-tag PAGE (Kinoshita et al. 15), & thus, molecular weight markers are not shown in the bottom gels. The red & black asterisks in (C) indicate phosphorylation of Parkin at Ser65 & an additional minor phosphorylation site, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23751051), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415384188.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - PINK1 translation is critical for the accumulation of PINK1 upon mitochondrial damage. aPINK1 mRNA levels were not altered by mitochondrial damage. HeLa cells were incubated with 10 μM CCCP or combination of 10 μM oligomycin & 4 μM antimycin for 2 h & then, PINK1 mRNA levels were measured by qRT-PCR. PINK1 mRNA level was normalized to the corresponding GAPDH levels & quantified as a percentage of control (n = 8, t-test). b, c Translation is involved in PINK1 accumulation upon CCCP treatment. HeLa cells were first pre-incubated with 100 μM cycloheximide (CHX) for 2 h & then washed. Cells were further incubated with 10 μM CCCP with or without CHX for 2 h (n = 4, two-way ANOVA). Values are mean ± SEM (n.s. = non-significant, ***p 1000 cells) & are shown as a percentage of control. Values are mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27456084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415483619.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Inhibition of endogenous miR-27a/b function increases human PINK1 expression. a Schematic diagram of sequence alignment of miR-27a/b & anti-miR-27a/b. Asterisks (*) indicate conserved nucleotides. b Inhibition of endogenous miR-27a/b function increased luciferase activities in HeLa cells. Cells were transfected with the reporter constructs containing the full-length 3′UTR of human PINK1 mRNA with wild-type (WT) or mutated (Mut) seed match sites downstream of Renilla luciferase gene. 48 h post-transfection, luciferase activities were measured (n = 4, t-test). c-f Inhibition of endogenous miR-27a/b increased PINK1 protein levels in HeLa (C-D) & M17 cells (E-F). Cells were transfected with 150 nM of anti-miR-27a/b or anti-control (anti-Ctl). 48 h post-transfection, cells were harvested for Western blot. PINK1 protein level was normalized to corresponding GAPDH level & quantified as a percentage of control (n = 4, t-test). Data are shown as a percentage of control. Values are mean ± SEM (n.s. = non-significant, **p < 0.01, ***p < 0.00) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27456084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482287.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Regulation of PINK1 by miRNA. a Schematic diagram of AGO-specific RNA immunoprecipitation assay. b, cPINK1 mRNAs are associated with miRNA/RISC complex. HeLa cells were transfected with 50 nM of AGO2 siRNA or negative control. 48 h post-transfection, AGO2-bound mRNAs were pulled down from cell lysates with 2A8 anti-AGO2 antibody or non-specific IgG antibody. PINK1 mRNA levels were then measured by qRT-PCR. siRNA against AGO2 served as a quality control for AGO-specific RNA immunoprecipitation assay. PINK1 mRNA levels were quantified as a percentage of group 1 (n = 6, two-way ANOVA). d, e Knock-down of DICER1 increased PINK1 protein levels. HeLa cells were transfected with 50 nM DICER1 siRNA (DICER1 KD) or negative control (Ctl). 48 h post-transfection, cells were harvested for Western blot. The arrowhead indicates a full-length PINK1 band & the asterisk indicates a non-specific band. Each protein level was normalized to corresponding GAPDH level & quantified as a percentage of control (n = 4, t-test). Values are mean ± SEM (n.s. = non-significant, **p < 0.01, ***p < 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27456084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482296.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON) or mechanical ventilation at high tidal volume (HTV) with saline, antimycin A (AmA) or cyclosporine A (CsA). (A) Levels of PINK1, Parkin, & Mfn1 mRNA. (B) Levels of LC3B, PINK1, Parkin, & Mfn1 protein by Western blot. (C) Relative expression of LC3B-II/LC3B-I & PINK1 protein. (D) Relative expression of Parkin & Mfn1 protein. (E) Levels of TLR9 & COX4 mRNA. (F) Levels of MyD88 & nuclear factor-kappa B (NF-kappa B) mRNA. (G) Levels of TLR9, COX4, MyD88, & NF-kappa B protein by Western blot. (H) Relative expression of TLR9 & COX4 protein. (I) Relative expression of MyD88 & NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group;bP < 0.05 vs. HTV group; & cP < 0.05 vs. AmA group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415485118.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Mitophagy contributes to removal of excess mitochondrial fragments in preeclampsia.(a, left panel) Representative TEM image of a cytotrophoblast from PTC & PE placenta collected at 29 weeks. Mitochondrial proximity to ER (MAM) is indicated by white arrows, mitochondrial fission events are depicted by white stars (scale bar: 500 nm; n = 8 separate PE placentae). (a, right panel) percentage of mitochondrial tethering to the ER in in PE vs. PTC (PE placentae, n = 8; PTC placentae, n = 7; unpaired Student’s t-test *P < 0.05). b Representative western blots for MFN2 & calreticulin in ER isolated from PE & PTC placentae (PE & PTC, n = 3 separate samples). c WB & associated densitometry of ASAH2 (normalized to TOM20) in mitochondria from PTC & PE placentae. d Representative TEM depicting mitophagy (white arrow) in cytotrophoblast cell from PE placenta (scale bar: 500 nm, n = 8 separate PE placentae). e Western blot & associated densitometry of PINK1 & Parkin in PE vs. PTC mitochondrial isolates. Densitometry for PINK1 blot was used to calculate the ratio of full-length PINK63kDa to cleaved PINK153kDa (PE & PTC, n = 4 separate placentae, *P < 0.05) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-018-0360-0), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482279.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482299.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Polyphyllin I suppressed, & PINK1 knockdown further suppressed, tumor growth in a MDA-MB-231 xenograft modelA. Tumor volumes were measured every week & differed at the end of the treatment period (*P < 0.01 compared to shCon cells treated with PPI, ##P< 0.01 compared to shPINK1 cells treated with PPI). B. Representative image of tumors from each group. C. Body weight changes in mice during the 6 weeks of PPI treatment. There were no differences in body weights between the PPI-treated shCon & vehicle-treated shCon groups or between the PPI-treated shPINK1 group & shCon groups. D. Representative tumor tissues were sectioned & subjected to H&E staining, TUNEL assay, & immunohistochemistry staining for C-CASP3. Scale bars: 50 μm. E. Representative tumor tissues from each group were prepared & subjected to western blot using anti-DRP1, -PARK2, -LC3B, -PINK1, & -C-CASP3 antibodies. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.14413), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415483648.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Corticosterone affects NIX-dependent mitophagy through decreasing PGC1 alpha in vivo.a–f Mice exposed to vehicle, corticosterone (10 mg/kg), corticosterone w/ phorbol 12-myristate 13-acetate (PMA pretreatment, 200 μg/kg), or PMA alone for 7 days. a Slide samples for IHC immunostained w/ LAMP1 (green), TOMM20 (red), & DAPI (blue). Scale bars, 100 μm (magnification, ×200). n = 5. b The expressions of NIX, PTEN-induced kinase 1 (PINK1), & BCL2 interacting protein 3 (BNIP3) detected w/ WB where beta -actin used as a loading control. n = 5. c Slide samples for IHC immunostained w/ synpatophysin (green), PSD95 (red), & DAPI (blue). Scale bars, 100 μm (magnification, ×200). n = 5. d Synaptophysin & PSD95 detected by WB. Loading control is beta -actin. n = 5. e The mice subjected to Y-maze test to evaluate spatial memory function. n = 6. f The mice subjected to forced swim test to evaluate depression-like behavior. n = 5. g Vehicle or RU 486 (5 mg/kg) injected mice presented w//w/out corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha ) & NIX visualized via WB. Loading control is beta -actin. n = 5. h The schematic model for mechanisms of inhibition in NIX-dependent mitophagy by glucocorticoid shown. All blots & IF images representative. n = 5 or 6 from each animal w/ two technical replicates each in results of IHC & WB. Quantitative data presented as a mean ± S.E.M. The representative images acquired by SRRF imaging system. Two-sided two-way ANOVA conducted except Fig. 8b, data of which analyzed by two-sided unpaired student’s t test. ** indicates p < 0.01 versus control & ## indicates p < 0.01 versus corticosterone, respectively. Data provided as a Source data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482218.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - G-TPP activity is conserved in primary fibroblasts(A, C) Fibroblasts were treated with 15 µM G-TPP for the indicated time points. Cells were harvested & western blots were probed with antibodies against (A) PINK1, pS65-Ub & total Ub or (C) autophagy adapter proteins. GAPDH & Vinculin served as loading control. G-TPP treatment led to PINK1 stabilization & pS65-Ub induction in primary skin fibroblasts. p62 levels were induced upon G-TPP treatment, while other adapters seemed decreased. (B, D) Human fibroblasts were treated with 15 µM G-TPP for 16 h & fixed & stained with antibodies against (B) pS65-Ub (green) or (D) the autophagy adapters NBR1, NDP52, p62, OPTN & TAX1BP1 (green). Mitochondria were stained with antibodies against TOM20 (red), nuclei were visualized with Hoechst (blue). Scale bars indicate 10 µM. A magnified image of the boxed region, the fluorescence profile along the arrow & the Pearson’s correlation coefficient of adapter protein & mitochondrial stainingare shown to the right. Shown is the mean ± SEM of at least five randomly selected images (unpaired, two-sided t-test, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482286.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Polyphyllin I triggers PINK1/PARK2-dependent mitophagyA. MDA-MB-231 cells were treated with 8 μM PPI for different periods of time as indicated, & PARK2, P62, & ubiquitin (UB) levels in mitochondrial fractions were determined by western blot. B. Cells were cotransfected with GFP-UB & RFP-mito & treated with 8 μM PPI for 9 h, after which PARK2 (Alexa Fluor 405, pink) & P62 (Alexa Fluor 647, blue) immunostaining was detected using confocal microscopy. C. Cells cotransfected with GFP-UB & RFP-LC3 were treated with 8 μM PPI for 9 h, after which PARK2 (Alexa Fluor 405, pink) & TOMM20 (Alexa Fluor 647, blue) immunostaining was detected using confocal microscopy. Scale bars: 10 μm. D-E. MDA-MB-231 cells were treated with 8 μM PPI for different periods of time as indicated; whole-cell lysates were then separated on 8% SDS-PAGE gels & analyzed by western blot using the anti-PINK1 antibody. Relative full-length (∼63 kDa) & cleaved (∼52 kDa) PINK1 levels were quantified by densitometry & normalized to Tubulin. The results were expressed as a percentage of control, which was set at 100%. Data are presented as mean ± SD (*P< 0.01 vs. the control). F. Cells were treated with 8 μM PPI for different periods of time as indicated, & whole-cell lysates were then subjected to western blot analysis. G. Cells were treated with 8 μM PPI for 9 h, after which mitochondrial fractions were prepared & subjected to immunoprecipitation using anti-PINK1 antibody; associated PARK2 was detected using immunoblotting. H. RFP-mito-expressing MDA-MB-231 cells were treated with 8 μM PPI for 9 h, & PINK1 (Alexa Fluor 488, green) & PARK2 (Alexa Fluor 405, pink) immunostaining were evaluated using confocal microscopy. Scale bars: 10 μm. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.14413), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482214.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - miR-27a/b suppress human PINK1 expression. a Schematic diagram of the conserved target sites of miR-27a/b in the 3′UTR of human PINK1 mRNA. Seed sequences were indicated in red. Asterisks (*) indicate the conserved nucleotides. b Schematic diagram of the luciferase reporter plasmids. The reporter constructs contain the full-length 3′UTR of human PINK1 mRNA with wild-type (WT) or mutated (Mut) seed match sites downstream of Renilla luciferase gene. c Overexpression of miR-27a/b decreased luciferase activities in HeLa cells. Cells were transfected with miR-27a/b or negative control (Ctl) along with reporter constructs as indicated. 48 h post-transfection, luciferase activities were measured (n = 4, one-way ANOVA). Renilla luciferase activity was normalized to the corresponding firefly luciferase activity. d-g Overexpression of miR-27a/b decreased PINK1 protein levels in HeLa (D-E) & M17 cells (F-G). Cells were transfected with miR-27a/b or negative control (Ctl). 48 h post-transfection, cells were harvested for Western blot (n = 4, t-test). PINK1 protein level was normalized to corresponding GAPDH level & quantified as a percentage of control. Data are shown as a percentage of control. Values are mean ± SEM (n.s. = non-significant, **p < 0.01, ***p < 0.001) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27456084), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415483618.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - Increased p62 expression is prevented in PINK1 knock‐down cells. (a) SH‐SY5Y cells expressing scrambled shRNA or PINK1 shRNA (117‐2, 118‐3, & 118‐4) were treated with 10 μM carbonyl cyanide m‐chlorophenylhydrazone (CCCP) for 24 h & full‐length (FL) PINK1 protein levels measured by western blot. No accumulation of FL PINK1 was observed in 118‐3 & 118‐4 PINK1 shRNA clones. (b) SH‐SY5Y cells constitutively expressing scrambled (scram) or PINK1 shRNA (clone 118‐3) were treated with vehicle or 10 μM CCCP for 24 h & p62 protein expression measured by western blotting. **p < 0.05 versus scram CCCP; n = 9. (c) SH‐SY5Y cells were incubated in serum‐free culture media for up to 24 h to induce starvation‐mediated macroautophagy. p62 protein levels were measured by western blotting. *p < 0.05 versus 0 h; **p < 0.01 versus 0 h; n = 4. (d) SH‐SY5Y cells were transfected with scrambled (scram) or p62 siRNA for 72 h. For the last 24 h, cells were treated with vehicle (ethanol) or 10 μM CCCP. p62 protein expression was then measured by western blotting. p62 protein was significantly decreased in p62 siRNA cells under basal conditions & following CCCP treatment, when compared to scram cells. **p < 0.01 versus scram vehicle or scram CCCP; n = 3. (e) Mitochondrial content was measured in SH‐SY5Y cells treated with scram or p62 siRNA for 72 h by measuring citrate synthase (CS) activity. For the last 24 h, cells were treated with vehicle or 10 μM CCCP & data are expressed as % of vehicle. *p < 0.05 versus scram CCCP treatment; n = 5. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26509433), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482278.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - NF kappa B upregulates human PINK1 gene expression. (A) NF kappa B increases the endogenous human PINK1 mRNA level. HEK293 cells were transfected with either the NF kappa B expression vector or empty vector pMTF. RT-PCRs were performed using either primers specific to the human PINK1 coding sequence or the human beta -actin coding sequence. (B) NF kappa B increases the endogenous human PINK1 protein levels. HEK293 cells transfected with NF kappa B were harvested 48 h after transfection for protein detection. Cell lysates were run on 10% glycine gel & images were collected by Licor. A significant increase of endogenous PINK1 was observed. (C) The endogenous human PINK1 protein level was dramatically increased by NF kappa B in SH-SY5Y cells. SH-SY5Y cells were transfected with NF kappa B p65 plasmids or the control vector pMTF & then harvested for protein detections. The human PINK1 protein & its proteolysis products ∆1-PINK1 (55kD) & ∆2-PINK1 (45kD) were all increased. beta -actin acted as the internal control. (D) LPS treatment facilitated PINK1 expression in SH-SY5Y cells. Cells were harvested after being treated for 16 h & then subjected to Western blot. The human beta -actin level was served as a control. Quantification was performed by Image J software. Values indicate means ± SEM. n = 3, *p <0.01 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25108683), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415485116.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - G-TPP induces PINK1 stabilization & kinase activity in HeLa cells(A) G-TPP treatment leads to PINK1 stabilization & pS65-Ub induction in HeLa cells. HeLa cells stably expressing untagged Parkin were treated with 10 µM G-TPP for the indicated times. Western blots were prepared with cell lysates & probed with antibodies against PINK1 & pS65-Ub. GAPDH served as a loading control. (B) pS65-Ub is induced in G-TPP treated cells & co-localizes with EGFP-Parkin & mitochondria. HeLa cells stably expressing EGFP-Parkin (green) were treated with 10 µM G-TPP for the indicated times & fixed. Cells were stained with antibodies against pS65-Ub (red) & the mitochondrial marker TOM20 (cyan). Scale bars correspond to 10 µM. (C) Quantification of Parkin translocation using High Content Imaging. HeLa EGFP-Parkin cells were treated for 4 or 8 h with or without 10 µM G-TPP. CCCP treatment (10 µM for 2 h) was used as a positive control. Cells were fixed, counterstained with Hoechst dye to visualize nuclei, imaged & analyzed using the ratio of cytoplasmic to nuclear EGFP signal [21]. Data was normalized to positive (2 h 10 µM CCCP treatment) & negative (2 h DMSO) controls. G-TPP significantly induced Parkin re-localization to levels similar to or beyond 2 h CCCP treatment. Shown are the mean values of three independent experiments with triplicate wells each ± SEM (one-way ANOVA with Tukey’s posthoc, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-31020241548369.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - NF kappa B upregulates human PINK1 gene expression. (A) NF kappa B increases the endogenous human PINK1 mRNA level. HEK293 cells were transfected with either the NF kappa B expression vector or empty vector pMTF. RT-PCRs were performed using either primers specific to the human PINK1 coding sequence or the human beta -actin coding sequence. (B) NF kappa B increases the endogenous human PINK1 protein levels. HEK293 cells transfected with NF kappa B were harvested 48 h after transfection for protein detection. Cell lysates were run on 10% glycine gel & images were collected by Licor. A significant increase of endogenous PINK1 was observed. (C) The endogenous human PINK1 protein level was dramatically increased by NF kappa B in SH-SY5Y cells. SH-SY5Y cells were transfected with NF kappa B p65 plasmids or the control vector pMTF & then harvested for protein detections. The human PINK1 protein & its proteolysis products ∆1-PINK1 (55kD) & ∆2-PINK1 (45kD) were all increased. beta -actin acted as the internal control. (D) LPS treatment facilitated PINK1 expression in SH-SY5Y cells. Cells were harvested after being treated for 16 h & then subjected to Western blot. The human beta -actin level was served as a control. Quantification was performed by Image J software. Values indicate means ± SEM. n = 3, *p <0.01 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25108683), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482277.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - NF kappa B upregulates human PINK1 gene expression. (A) NF kappa B increases the endogenous human PINK1 mRNA level. HEK293 cells were transfected with either the NF kappa B expression vector or empty vector pMTF. RT-PCRs were performed using either primers specific to the human PINK1 coding sequence or the human beta -actin coding sequence. (B) NF kappa B increases the endogenous human PINK1 protein levels. HEK293 cells transfected with NF kappa B were harvested 48 h after transfection for protein detection. Cell lysates were run on 10% glycine gel & images were collected by Licor. A significant increase of endogenous PINK1 was observed. (C) The endogenous human PINK1 protein level was dramatically increased by NF kappa B in SH-SY5Y cells. SH-SY5Y cells were transfected with NF kappa B p65 plasmids or the control vector pMTF & then harvested for protein detections. The human PINK1 protein & its proteolysis products ∆1-PINK1 (55kD) & ∆2-PINK1 (45kD) were all increased. beta -actin acted as the internal control. (D) LPS treatment facilitated PINK1 expression in SH-SY5Y cells. Cells were harvested after being treated for 16 h & then subjected to Western blot. The human beta -actin level was served as a control. Quantification was performed by Image J software. Values indicate means ± SEM. n = 3, *p <0.01 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25108683), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-31020241548363.jpg)
![Western Blot: PINK1 Antibody - BSA Free [BC100-494] - G-TPP leads to recruitment of autophagy adapters & degradation of mitochondria(A) HeLa cells stably expressing untagged Parkin were treated with 10 µM G-TPP for 8 h. Western blots were prepared from cell lysates & probed with antibodies against LC3, phospho-TBK1 (Ser172) & TBK1. GAPDH was used as a loading control. Upon 8 h the levels of LC3-I & LC3-II were both increased. At 8 h after treatment with G-TPP but not at 4 or 24 h, TBK1 was phosphorylated. (B) HeLa cells stably expressing EGFP-Parkin were treated with 10 µM G-TPP & fixed 8 h after treatment. Cells were stained with antibodies against the autophagy adapter proteins NBR1, NDP52, OPTN, p62, & TAX1BP1 (red). Mitochondria were counterstained with TOM20 antibodies (cyan), nuclei with Hoechst (blue). EGFP-Parkin epifluorescence is shown in green. Scale bar corresponds to 10 µM. (C) HeLa cells stably expressing EGFP-Parkin & the reporter protein mitoKeima were treated with 10 µM CCCP or G-TPP & imaged over time. The ratio of ‘neutral’ mitoKeima to ‘acidic’ mitoKeima was calculated as readout for mitophagy. Parkin translocation was monitored at the same time. Values for Parkin translocation & mitophagy were normalized to 12 h treatment with 10 µM CCCP as positive control & DMSO as negative control (two-way ANOVA with Tukey’s post-hoc test, **p < 0.005, ***p < 0.0005). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22287), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/bc100-494_rabbit-polyclonal-pink1-antibody-310202415482225.jpg)
![Western Blot: Rabbit Polyclonal PINK1 Antibody [BC100-494] - PINK1 detection in mouse and postmortem human cortex samples. 1:1000 dilution at 4 degrees overnight to detect endogenous PINK1 from mouse and postmortem human cortex samples (10 ug total protein). Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/bc100-494_rabbit-polyclonal-pink1-antibody-western-blot-231202510057..jpg)
![Western Blot: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Knockdown-Validated-NB600-101-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was conjugated with Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhus University Hospital.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells of blood vessels (not the RBCs though) and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunohistochemistry-Paraffin-NB600-101-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - The Calreticulin antibody NB600-101 was tested in HeLa cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0007.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunohistochemistry-Paraffin-NB600-101-img0009.jpg)
![Simple Western: Calreticulin Antibody [NB600-101] - Lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Simple-Western-NB600-101-img0008.jpg)
![Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of whole cell lysates from HeLa, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated a specific band of Calreticulin protein at ~48 kDa molecular weight position.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Western-Blot-NBP1-47518-img0017.jpg "Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of whole cell lysates from HeLa, A549, NTERA2 and MCF-7 using Calreticulin antibody clone 1G6A7. The antibody generated a specific band of Calreticulin protein at ~48 kDa molecular weight position.")
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - Intracellular staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Calreticulin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Antibody was tested in HeLa cells at a 1:250 against DyLight 488 (Green). Actin was counterstained against Phalloidin 568 (Red) and cells were mounted in DAPI Flouromount (Blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0008.jpg)
![Western Blot: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis using Calreticulin mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Western-Blot-NBP1-47518-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of paraffin-embedded human placenta tissues using anti-Calreticulin mAb.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunohistochemistry-Paraffin-NBP1-47518-img0002.jpg)
![Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - An intracellular stain was performed on A431 cells with Calreticulin (1G6A7) Antibody NBP1-47518 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of 3T3-L1 cells using anti-Calreticulin mAb (green). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (1G6A7) [NBP1-47518] - Analysis of SKBR-3 (left) and A549 (right) cells using anti-Calreticulin mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Immunocytochemistry-Immunofluorescence-NBP1-47518-img0007.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - A549 cells stained with NBP1-47518PCP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to PerCP.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0026.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - U-937 cells stained with NBP1-47518F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0027.jpg)
![Flow (Intracellular): Calreticulin Antibody (1G6A7) [NBP1-47518] - Jurkat cells stained with NBP1-47518APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0028.jpg)
![Flow Cytometry: Calreticulin Antibody (1G6A7) [NBP1-47518] - An intracellular stain was performed on HeLa cells with Calreticulin Antibody [1G6A7] NBP1-47518AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-1G6A7-Flow-Cytometry-NBP1-47518-img0034.jpg)
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0001.jpg "Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - 10 ug of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.")
![Western Blot: NMDAR1 Antibody (R1JHL) [NB300-118] - The blots show the examples of immunobands against NMDAR1 and beta-actin antibodies on the membrane, which was performed on the membrane protein prepared from the lumbar spinal cords of the offspring (P14) rats in different groups. Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/18/1/64) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Western-Blot-NB300-118-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - NR1 surface staining of mouse corical neruons. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mouse neuron stained with anti-NMDAR1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR1 Antibody (R1JHL) [NB300-118] - Mixed rat cortical cultures (21 DIV) were labelled with anti-NMDAR1 (1:200) and anti-mouse CF568 (1:250). The antibody labels small punctae along the neuronal processes. No distinct features such as spines are visible. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/NMDAR1-Antibody-R1JHL-Immunocytochemistry-Immunofluorescence-NB300-118-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: NMDAR1 Antibody (R1JHL) - Azide and BSA Free [NB300-118] - Notch1 colocalizes postsynaptically with Reelin signaling components. (A) Representative IEM images from hippocampal slices using an antibody specific for Notch1 show that gold particles are localized at postsynaptic as well as presynaptic membrane terminals. (B) Bar graph summarizing the counting of gold particles on the length of presynaptic & postsynaptic membranes indicates that the majority of the Notch1 gold particles are localized postsynaptically (5*10−3 ± 0.8*10−3 vs. 2*10−3 ± 0.4*10−3, n = 3 mice; Student’s t-test, p < 0.01). (C) Fluorescent immunolabeling on 14 days primary neuronal WT cultures shows colocalization of Notch1 & ApoER2 in soma & processes of pyramidal neurons. Both Notch1 & ApoER2 are similarly co-expressed in NMDAR1 positive puncta (R = 0.92 ± 0.03 & R = 0.86 ± 0.08, respectively; Student’s t-test, p = 0.23). (C′) Close up of a dendrite displaying Notch1, ApoER2 & NMDAR1 expression & “zoom in” captions of puncta showing clustering of the three receptors in teal. (D) Fluorescent immunolabeling on primary neuronal cultures shows that Notch1 & Dab1 localize in the same pyramidal neuron’s soma & processes labeled by phalloidin (R = 0.87 ± 0.06 & R = 0.74 ± 0.07 respectively; Student’s t-test, p = 0.5). (D′) Close up of a dendrite with Notch1, Dab1 & F-actin labeling & “zoom in” captions of dendritic puncta showing clustering of Notch1, Dab1 & F-actin in teal. **p < 0.01. Error bars are SEM & scale bar in (A) is 200 nm for all IEM panels, in (C,D) 50 μm & in (C′,D′) 10 μm & 500 nm in zoom in captions. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-118_mouse-monoclonal-nmdar1-antibody-r1jhl-imgenex-img-5000-31020241620518.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Knockdown-Validated-NB600-103-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Calreticulin Antibody [NB600-103] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-103) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Simple Western: Calreticulin Antibody [NB600-103] - Lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Simple-Western-NB600-103-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-103] - IF Confocal analysis of 3T3 cells using Calreticulin antibody (NB600-103, 1:20). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-103-img0005.jpg)
![Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - Analysis of FFPE human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in glands was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Immunohistochemistry-Paraffin-NB600-103-img0007.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB600-103 at 1:1,000. ECL exposure, 10 seconds.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0003.jpg)
![Western Blot: Calreticulin Antibody [NB600-103] - Analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.](http://images.novusbio.com/fullsize/Calreticulin-Antibody-Western-Blot-NB600-103-img0004.jpg)
![Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB100-493-img0003.jpg "Western Blot: PINK1 Antibody [NB100-493] - Detection of murine PINK1 using NB100-493. Lane 1: molecular weight marker. Lane 2. MES cell Mitochondria (20 ug) with a band at the observed molecular weight of 63 kDa. Lane 3. MES cytosol (20 ug). Lane 4. MES nuclear (20 ug) as negative control.Lane 5. Purified human cytochrome C (0.1 ug) as PINK1 negative control.")
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Western-Blot-NBP2-67017-img0007.jpg "Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of Parkin on 293T cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded rat brain tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0004.jpg)
![Flow Cytometry: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of SH-SY-5Y cells with Parkin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Flow-Cytometry-NBP2-67017-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Parkin Antibody (JF82-09) [NBP2-67017] - Staining Parkin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunocytochemistry-Immunofluorescence-NBP2-67017-img0006.jpg)
![Immunohistochemistry-Paraffin: Parkin Antibody (JF82-09) [NBP2-67017] - Analysis of paraffin-embedded mouse testis tissue using anti-Parkin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Parkin-Antibody-JF82-09-Immunohistochemistry-Paraffin-NBP2-67017-img0003.jpg)
![Western Blot: Parkin Antibody (JF82-09) [NBP2-67017] -Analysis of Parkin on different lysates with Rabbit anti-Parkin antibody at 1/1,000 dilution.Lane 1: 293 cell lysateLane 2: Jurkat cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 52 kDaObserved band size: 52 kDaExposure time: 1 minutes;10% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67017_rabbit-monoclonal-parkin-antibody-jf82-09-western-blot-1962024104114..jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0002.jpg "Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Simple Western: PINK1 Antibody [NBP1-49678] - Lane view shows a specific band for PINK1 at a dilution of 1:50 in 1.0 mg/ml of HeLa lysate. Molecular weight ~61kDa. This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PINK1-Antibody-Simple-Western-NBP1-49678-img0006.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NBP1-49678] - Stain in paraffin embedded mouse brain.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NBP1-49678-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NBP1-49678] - PINK1 antibody was tested at 1:50 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49678-img0005.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0014.jpg "Western Blot: Caveolin-2 Antibody [NBP1-31116] - Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Caveolin-2 Antibody [NBP1-31116] - FaDu xenograft, using Caveolin 2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-31116-img0006.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0008.jpg)
![Western Blot: Caveolin-2 Antibody [NBP1-31116] - Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Caveolin 2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Western-Blot-NBP1-31116-img0013.jpg)
![Immunoprecipitation: Caveolin-2 Antibody [NBP1-31116] - IP samples: A549 whole cell extract A. 30 ug A549 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of caveolin 2 protein by 4 ug Caveolin 2 antibody 15 % SDS-PAGE The immunoprecipitated caveolin 2 protein was detected by Caveolin 2 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/Caveolin-2-Antibody-Immunoprecipitation-NBP1-31116-img0012.jpg)
![Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.](http://images.novusbio.com/fullsize/PINK1-Antibody-Western-Blot-NB600-973-img0001.jpg "Western Blot: PINK1 Antibody [NB600-973] - Lane 1: The primary band with the observed molecular weight of 66 kDa was detected in mouse liver (30ug). Also, a truncated form of the protein at about 33 kDa was detected.")
![Immunocytochemistry/Immunofluorescence: PINK1 Antibody [NB600-973] - LRRK2 accumulates in globules in alphaS tg mice. Double immunofluorescence for alphaS with parkin, PINK1, DJ-1, LRRK2, or negative control (the immunopeptide-preabsorbed anti-LRRK2 antibody) in alphaS tg mice. Scale bar = 5 um for all panels. Image collected and cropped by CiteAb from the following publication (//molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-34) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-973-img0003.jpg)
![Immunohistochemistry-Paraffin: PINK1 Antibody [NB600-973] - Staining in human liver tissue using a 1:200 dilution.](http://images.novusbio.com/fullsize/PINK1-Antibody-Immunohistochemistry-Paraffin-NB600-973-img0002.jpg)
![Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).](http://images.novusbio.com/fullsize/Parkin-Antibody-PRK8-Western-Blot-NBP2-29838-img0001.jpg "Western Blot: Parkin Antibody (PRK8) [NBP2-29838] - HEK293 cell lysate was probed with anti- Parkin, clone PRK8 (1:2000 dilution). Arrow indicates Parkin (~52kDa).")
![Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S beta 7.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Western-Blot-NBP2-01832-img0025.jpg "Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S beta 7.")
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0023.jpg)
![Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 20S beta 7 monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Western-Blot-NBP2-01832-img0013.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0014.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0015.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0017.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0019.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0020.jpg)