| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-229SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-228SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24587-25ul | |||
| Azide and BSA Free | NBP3-43855 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37888-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24588-25ul | |||
| Azide and BSA Free | NBP3-43856 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90821-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84063-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84608-25ul | |||
![Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SCP1-Antibody-Simple-Western-NB300-229-img0010.jpg "Simple Western: SCP1 Antibody [NB300-229] - Simple Western lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: SCP1 Antibody [NB300-229] - Meiotic chromosome spreads from wild type and Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) and SYCP1 (green), which are axial element and central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) and abnormal SYCP3 structures (arrows). Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice. PLoS One (2016)](http://images.novusbio.com/fullsize/SCP1-Antibody-Western-Blot-NB300-229-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) and SCP1 (red). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0017.jpg)
![Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunohistochemistry-Paraffin-NB300-229-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-229-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SCP1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB300-229-img0016.jpg)
![Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] - Meiotic spreads.Meiotic chromosome spreads from wild type & Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) & SYCP1 (green), which are axial element & central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) & abnormal SYCP3 structures (arrows). Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0159800), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-229_rabbit-polyclonal-scp1-antibody-310202415345326.jpg)
![Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic progression & chromosome paring in XX, XO & XY oocytes.(A) Meiotic progression in XX, XO & XY oocytes. The graphs show the percentages of the meiotic stage at the day of culture indicated. L, leptotene; Z, zygotene; P, pachytene; D, diplotene. (B) Pairing of homologous chromosomes in XX, XO & XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) & SYCP1 (red), & FISH analysis of the X chromosome (green) & Y chromosome (purple). The dashed squares in the merged images are shown at high magnification (right). The numbers of samples showing the phenotype are shown with the total number tested (left). Arrowheads indicate asynapsed bivalents at the end of the chromosomes. Scale bars, 10 μm. (C) Pairing rates of autosomes & sex chromosomes. Each value was calculated from three independent experiments (see also Materials & Methods). P values were calculated by Tukey’s HSD test. ***P600 spermatocytes in each stage. b, c The immunostaining of SYCP3 in the testicular sections (b) & surface-spread chromatin preparations of Uhrf1 deletion & control mice (c); d the percentage of spermatocytes with abnormal SYCP3 location. e Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) & SYCP1 (red). f The percentage of spermatocytes with abnormal SYCP1 location. Lep leptotene, Zyg zygotene, Pac pachytene, Dip diplotene. Data are presented as mean ± SEM of three mice. ***p ≤ 0.001. Scale bar, 25 μm in b, 5 μm in c, e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-229_rabbit-polyclonal-scp1-antibody-310202416212369.jpg)
![Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).](http://images.novusbio.com/fullsize/SCP1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-228-img0003.jpg "Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-228] - SCP1 labeled in mouse pachytene preparation (red), using NB 300-228. CDK2 staining, near teleomeres, is also present (green).")
![Simple Western: SCP1 Antibody [NB300-228] - Lane view shows a specific band for SCP1 in 0.5 mg/ml of Human Testis (left) and Mouse Testis (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/SCP1-Antibody-Simple-Western-NB300-228-img0006.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Western-Blot-NBP2-19952-img0004.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - Immunoblots of parental HCT116 cells and two clonal cell lines (clone 14 and clone 13) generated by CRISPR-mediated truncation of hRpn10. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 was knocked down in HCT116 cells by four different siRNAs and the cell lysates immunoprobed as indicated. Mock and scrambled control samples are included. b-actin is used as a loading control. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15073-7), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-Knockdown-Validated-NBP2-19952-img0003.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-37888-img0006.jpg "Western Blot: S5a/Angiocidin Antibody [NBP2-37888] - Analysis in control (vector only transfected HEK293T lysate) and PSMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP2-37888] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-37888-img0005.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human liver.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0010.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PSMD4 antibody NBP2-37888 (A) shows similar protein distribution across tissues to independent antibody NBP1-90821 (B).](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0007.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0008.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human testis.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0009.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP2-37888] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP2-37888-img0011.jpg)
![Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/MSH3-Antibody-Immunohistochemistry-Paraffin-NBP2-19417-img0007.jpg "Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human lung cancer. MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - Human colon carcinoma, using MSH3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MSH3-Antibody-Immunohistochemistry-Paraffin-NBP2-19417-img0002.jpg)
![Western Blot: MSH3 Antibody [NBP2-19417] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-91020242041224.jpg)
![MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MSH3 stained by MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:500.Blue: Fluoroshield with DAPI .Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-immunocytochemistry-immunofluorescence-2611202518402224.jpg)
![A549 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MSH3 antibody [C2C3], C-term (NBP2-19417) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19417_rabbit-polyclonal-msh3-antibody-western-blot-2611202518202319.jpg)
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP1-90821] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90821-img0007.jpg "Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody [NBP1-90821] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human liver.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0015.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0010.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining in human testis and pancreas tissues using anti-PSMD4 antibody. Corresponding PSMD4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human cerebral cortex, liver, lymph node and testis using Anti-PSMD4 antibody NBP1-90821 (A) shows similar protein distribution across tissues to independent antibody NBP2-37888 (B).](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0013.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0014.jpg)
![Immunohistochemistry-Paraffin: S5a/Angiocidin Antibody [NBP1-90821] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunohistochemistry-Paraffin-NBP1-90821-img0016.jpg)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0011.jpg "Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts and leydig cells.")
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Cerebellum shows moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0008.jpg)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0009.jpg)
![Immunohistochemistry-Paraffin: SRCAP Antibody [NBP1-84063] - Staining of human Skin shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SRCAP-Antibody-Immunohistochemistry-Paraffin-NBP1-84063-img0010.jpg)
![Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RBX1-Antibody-Western-Blot-NBP2-20113-img0001.jpg "Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RBX1 Antibody [NBP2-20113] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (NBP2-20113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20113_rabbit-polyclonal-rbx1-antibody-910202420104483.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0009.jpg "Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Analysis in human testis and liver tissues using NBP1-84608 antibody. Corresponding FANCI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0006.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0007.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human Rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0008.jpg)
![Immunohistochemistry-Paraffin: FANCI Antibody [NBP1-84608] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FANCI-Antibody-Immunohistochemistry-Paraffin-NBP1-84608-img0010.jpg)
![Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Western-Blot-NBP3-16240-img0002.jpg "Western Blot: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Western blot analysis of extracts of various cell lines, using S5a/Angiocidin Rabbit mAb (NBP3-16240) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb (NBP3-16240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/S5a-Angiocidin-Antibody-5G1X6-Immunocytochemistry-Immunofluorescence-NBP3-16240-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: S5a/Angiocidin Antibody (5G1X6) [NBP3-16240] - Immunofluorescence analysis of NIH-3T3 cells using S5a/Angiocidin Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16240_rabbit-s5a-angiocidin-mab-5g1x6-5220251255539.jpg)